LOCUS       MR216110                9192 bp    DNA     circular  SYN 
DEFINITION  Fyco1 (Myc-DDK-tagged) - Mouse FYVE and coiled-coil domain containing 1 (Fyco1), transcript variant 1
ACCESSION   NM_148925
VERSION     NM_148925.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5349..5440 
                     /label="Myc-DDK"  
     misc_feature    5470..6055 
                     /label="PolyA signal"  
     misc_feature    5527..5546 
                     /label="Restriction Enzyme"  
     misc_feature    6331..7003 
                     /label="ColE1"  
     misc_feature    7433..8227 
                     /label="Kan/Neo"  
     misc_feature    8262..8564 
                     /label="SV40 ori"  
     misc_feature    8650..9127 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5331..5337 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5331 
                     /label="Fyco1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCTAGC AGCACTGAGA CTCAGCTCCA GAGGATCATC CGTGATCTAC 
     1081 AAGATGCAGC GACAGAACTA AGCCATGAAT TTAAGGAAGG CGGAGAGCCA ATCACAGATG 
     1141 ACAGCACCAG TTTGCACAAG TTTTCTTACA AACTTGAGTA TCTTCTGCAA TTTGATCAGA 
     1201 AGGAGAAGGC GAGCCTCCTG GGAAGCAAGA AAGACTACTG GGATTACTTT TGTGCGTGCC 
     1261 TAGCCAAGGT GAAAGGAGCA AATGATGGCA TTCGATTTGT CAGGTCCATC TCTGAGCTCC 
     1321 GAACATCTCT GGGGAAAGGG AGAGCCTTCA TTCGCTACTC TTTGGTGCAC CAGAGGCTGG 
     1381 CAGACACCTT GCAGCAGTGC TTCATGAACA CTAAAGTGAC CAGTGACTGG TATTATGCAA 
     1441 GAAGCCCCTT TCTGAAGCCA AAGCTGAGCT CTGACATTGT GGGTCAACTG TATGAGCTGA 
     1501 CTGAGGTTCA GTTTGATCTA GCACCAAGAG GCTATGACTT GGATGCCGCC TGGCCAACAT 
     1561 TTGCCAGGAG GACGCTAGCC ACCAGCACGT CTGCTTACAT GTGGAAACCC CCCAGCCGAA 
     1621 GCTCCAGCAT GAGTAGTTTA GTGAGCAACT ACTTGCAGAC TCAGGAGATG GCCTCCAGTC 
     1681 TTGACCTGAA TTGTTCTCTA AACAATGAAG CACTAGAAAG CTTCGACGAG ATGCGGCTGG 
     1741 AGCTGGACCA GTTGGAGGTT CGGGAGAAAC AGCTACAAGA ACGTGTACAG CAGCTAGACA 
     1801 GGGAGAACCA AGCACTGCGA ATGTTGGTCA GCAGACAAGG GGGGCAGCTT CAGGTAGAGA 
     1861 AGGAGATGGG GTACCTTGCA GTCGAGGACA GCATTGGCCT TGTGAGCCTG GTAGCAGAAC 
     1921 TCCAGAAGCA GGGGGACGTC AGCCAGGCCA CAGTGAAGAA GCTACAGTCA TGTCTGCAGG 
     1981 CCTTGGAGCT AAATGTAGAC AAGAAAGAGT ACAGTCCCTC AGCCTTGCAG CTTGAGAACA 
     2041 TGGCGAAGGA GCTTGACACT GTGAGAGGCT CCTTGGGTAG GGAGAACCAG CTCCTGGCCA 
     2101 GCCTTTCAGA GCGCCTTGCT AGGGCAGAGA AAGGGGAAAA GACACCTCCA GACACAGAGC 
     2161 TTCATCAGGA GCCAGTTCCT GCGGATCTGG TGCTCAAGTT CCAGGAGCTG AAGGGAAAAC 
     2221 TTCAAGCCCT AGAAGGAGAG AACACCGAGG CCCAGGAGCT CAACAGGCAG CAGAGTATCA 
     2281 AGCTAGAGCA ACTGGCCAAG GAACTACAGC TGAAGGAGGA GGCCCGGGCT AGCCTAGCAC 
     2341 ACTTAGTGAA GGATGTGGTC CCACTTCAGG AAGAGCTGTC TGGAAAGAAG CAAGAGTCAG 
     2401 CTCAGCTCCG ACGACAGCTT CAGGAATCCC TGGCCCACTT GAGCTCTGTG GAGGAGGAGC 
     2461 TGGCTGAAGC CAGGCAGCAG GAAAAGCAGC ATCGAGAAGA AAAGCAGCTA CTGGAGCAGG 
     2521 AAGCCACGTC TCTTACATGG CAGCTGCAGC TCCTAGAGAC CCAGCTAGGA CAGGTGAGTC 
     2581 AGCTTGTAAG TGACCTGGAG GAGCAGAAGA AGCAACTCAT GCAGGAGAGA GACCATCTCA 
     2641 GCCAGAGAGT GGGCACACTG GAGCAGCTAG CCGAGGTGCA TGGCCCGCCA CAGTCTGCGG 
     2701 AGATGCCGGA AAAGAGGCAG CAGTGCCTCC GGGAAGAACA GGTAAACAAT AGCACAGTGT 
     2761 CAGAGGCAGA GCAGGAGGAG TTGCAGAAGG AGCTGCAGAA CATGGTTGAC CGCAACCAGC 
     2821 TCCTCGAAGG CAAACTGCAG GCCTTGCAAA CTGACTATAA GGCACTGCAG CAGCGAGAGG 
     2881 CAGCAATCCA GGGCTCTTTA GCCTCCCTGG AAGCTGAACA GGCTAGCATC CGGCACCTGG 
     2941 GAAACCAGAT GGAAGCAAGC CTCCTGGCTG TGAAGAAAGC CAAAGAGACT ATGAAAGCCC 
     3001 AGGTGGCAGA GAAGGAGGCT GCCCTGCAGA GTAAGGAGAG TGAGTGCCAG AGGCTGCAGG 
     3061 AAGAGGCAGA TCAGTGCCGG CTTCAGGCAG AGGCCCAGGC CCAGGAGCTC AGAGCTCTTG 
     3121 AGAACCAGTG CCAGCAGCAG ATTCAGCTGA TTGAGGTCCT CTCTGCAGAG AAAGGCCAAC 
     3181 AGGGACTCAG CCTGCCCCAA GTCAACACAG ACCAACTGGC CCTGTCTCAA GCACAGCTGG 
     3241 AAATCCACCA GGGGGAAGCC CAGCGGTTAC AGAATGAGGT GGTGGACCTC CAGGCCAAGC 
     3301 TCCAGGTGGC CCTAGGTGAT CGAGACAAGT TACAGAGCCA GCTGGGTGTG GCTGAGACAG 
     3361 TTCTAAGGGA GCACAAGACC CTGGTGCAAC AACTGAAAGA GCAGAATGAA GCCCTCAACA 
     3421 GGGCCCATGT TCAGGAGCTG CTTCAGTGCT CAGAGAGAGA GGGGATACTA CAGGAGGAGA 
     3481 GCATCTACAA GGCCCAGAAG CAGGAACAAG AGCTACGAGC CCTGCAGGCA GAGCTGTCAC 
     3541 AGGTGAGGTG CAGCTCTGAG GGAGCCCACC TGGAACATGC AGAGCTGCAA GACCAGCTGC 
     3601 ACCGAGCCAA CACAGACACA GCTGAGCTTG GTATACAGGT CTGTGCACTG ACAGCTGAGA 
     3661 AGGATCGAAT GGAGGAGGCC CTGGCCAGCC TAGCCCAGGA GCTCCAGGAC TCCAAAGAGG 
     3721 CAGCACTACA GGAACGAAAG GGCTTGGAGC TCCAAGTGAT GCAACTTCAG CAAGAGAAGG 
     3781 AGAAGTTGCA GGAAAAGGTG AAGGCAGCTG AGGAGGCAGC CAGTTCATTC TCTGGTCTGC 
     3841 AGGCACAGCT GGCCCAGGCT GAGCAGCTAG CCCAGAGCCT CCAAGAGACT GCACACCAGG 
     3901 AACAAGATGC CCTCAAGTTC CAACTAAGTG CTGAGATTAT GGACCACCAG AACCGATTAA 
     3961 AGACAGCCAA TGAAGAGTGT GGGCACCTCA GGGCCCAGCT AGAAGAACAA GGCCAGCAGC 
     4021 TGCAAATGAC TAAGGAGGCT GTGCAGGAAC TGGAGATCAC CAAGGCGGCC ATGGAGGAGA 
     4081 AGCTGAATTG CACCAGTAGC CACCTTGCAG AGTGCCAGGC CACTTTACTG CGCAAAGATG 
     4141 AAGAAAGCAC TATGCTTCAA ACCAGTCTAG AAAGAACACA GAAGGAACTT GAAAAGGCTA 
     4201 CATCAAAAAT TCAAGAATAT TACAACAAAC TCTGCCAAGA GGTGACAAAC AGGGAAAGGA 
     4261 ATGACCAGAA GATGCTTGCA GACCTCGATG ACCTGAACAG AACCAAGAAA TACCTTGAGG 
     4321 AGCGGCTGAT AGAGCTGCTC AGGGACAAAG ATGCTCTCTG GCAAAAATCC GATGCACTGG 
     4381 AATTCCAGCA GAAGCTCAGT GCTGAAGAGA AATGTCTTGG GGACATGGAA GTCAACCACT 
     4441 GCCATGACTG CAAGCGGGAG TTCAGCTGGA TAGTGCGACG GCACCACTGC AGGATATGTG 
     4501 GCCGTATCTT CTGTTACTAC TGCTGTAATA ACTATGTTGT GACTAAGCCC AGTGGCAAGA 
     4561 AGGAACGCTG CTGCCGAGCC TGCTTCCAGA AGTTCGGCGA AGGCTCTGGA TCCAATGATA 
     4621 GCAGTGGTTC AGGCACTAGC CAGGGAGAGC CCAGCCCCAT GGTGTCACCA GCTGAAGCAA 
     4681 GTCCCCAGTC CATAGGAAGC CAAGGGATAA ACTCAGTCTG CAGACCACCA GACGATGCTG 
     4741 TGTTTGACAT CATCACTGAT GAAGAACTGT GCCAGATCCA AGAATCTGGC TCCTCCTTGC 
     4801 CTGAAACACC CACTGAAACT GATTCAATGG ACCCGAATAC GGCTGAACAG GACACCACAT 
     4861 CAAACTCATT AACCCCTGAA GACACTGAAG ACGTGCCCAT GGGGCAAGAT GCTGAAATCT 
     4921 GCTTGCTGAA GTCAGGAGAG CTGATGATCA AATTACCCCT CACAGTAGAG GAGGTCGCCA 
     4981 GCTTCGGGGA GGGCAGCAGG GAGCTGTTTG TGAGGTCCAG TACCTACAGC CTGATCACCA 
     5041 TCACCGTGGC TGAGCCTGGC CTCACCATCA GCTGGGTCTT CTCTTCTGAT CCCAAGAGCA 
     5101 TCTCCTTCAG TGTGGTCTTC CAAGAGACAG AAGACACGCC GCTCGACCAG TGCAAGGTCC 
     5161 TCATTCCCAC CACCCGATGC AATTCCCACA AGGAGAACAT CCGGGGCCAG CTGAAGGTCC 
     5221 GAATCCCTGG CATCTACTTG CTCATCTTTG ACAACACCTT TTCAAGATTT ATCTCCAAAA 
     5281 AAGTGCTTTA CCACCTGACT GTTGACCGGC CTGTGATCTA CGATGGAAGC GATTTCCCAA 
     5341 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5401 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5461 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5521 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5581 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5641 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5701 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5761 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5821 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5881 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5941 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6001 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6061 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6121 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6181 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6241 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6301 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6361 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6421 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6481 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6541 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6601 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6661 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6721 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6781 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6841 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6901 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6961 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7021 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7081 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7141 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7201 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7261 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7321 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7381 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7441 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7501 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7561 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7621 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7681 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7741 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7801 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7861 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7921 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7981 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8041 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8101 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8161 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8221 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8281 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8341 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8401 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8461 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8521 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8581 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8641 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8701 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8761 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8821 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8881 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8941 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9001 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9061 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9121 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9181 AACGCGAATT TT
//