LOCUS       MR216109                8250 bp    DNA     circular  SYN 
DEFINITION  Pelp1 (Myc-DDK-tagged) - Mouse proline, glutamic acid and leucine rich protein 1 (Pelp1)
ACCESSION   NM_029231
VERSION     NM_029231.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4407..4498 
                     /label="Myc-DDK"  
     misc_feature    4528..5113 
                     /label="PolyA signal"  
     misc_feature    4585..4604 
                     /label="Restriction Enzyme"  
     misc_feature    5389..6061 
                     /label="ColE1"  
     misc_feature    6491..7285 
                     /label="Kan/Neo"  
     misc_feature    7320..7622 
                     /label="SV40 ori"  
     misc_feature    7708..8185 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4389..4395 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4389 
                     /label="Pelp1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCC GTTCTTAGTG GGGCCTCTGC GGGCTCCCCA GCTGGGGCTC 
     1081 CTGGCGGGCC CGGGGGTCTC TCTGCGGTGG GATCCGGCCC GCGTCTGCGT CTGCTGCTGT 
     1141 TAGAGAGCAT TTCCGGGTTG TTACAGCCGC GAACGGCGTC CCCCGTGGCC CCTGTGCATC 
     1201 CCCCAATCCA GTGGGCCCCG CATTTGCCCG GGCTCATGTG CTTATTGAGG CTGCATGGGA 
     1261 CGGCAGGTGG GGCGCAGAAT CTTTCAGCTC TTGGGGCATT GGTGAATCTC AGTAATGCAC 
     1321 ATCTTGGCTC CATCAAAACT CGGTTTGAAG GCCTGTGTCT GCTGTCCTTG TTGATAGGGG 
     1381 AGAGCCCCAC AGAGTTATTC CAGCAACATT GTGTTTCTTG GCTTCGGAGC ATCCAGCAGG 
     1441 TGCTGCAGTC TCAGGACTCA CCGTCCACCA TGGAGCTGGC TGTGGCTGTC CTGAGGGATC 
     1501 TGCTCCGACA TGCATCCCAG CTTCCTACCC TGTTTCGGGA CATCTCCACC AACCACCTTC 
     1561 CTGGGCTTCT TACTTCCTTG CTGGGCCTCA GACCAGAGTG TGAACAATCA GCTTTGGAGG 
     1621 GAATGAAGGC TTGTGTGACC TATTTTCCTC GGGCCTGTGG CTCTCTAAAA GGAAAGCTGG 
     1681 CTTCCTTTTT CCTGTCTCGA TTGGATTCCC TGAACCCTCA GCTCCAACAG CTGGCCTGCG 
     1741 AGTGTTACTC CAGGTTGCCC TCGTTAGGTG CTGGCTTTTC CCAGGGACTG AAGCACACAG 
     1801 AGAACTGGGA ACAGGAGCTG CACAGCCTGC TGACCTCACT GCACAGCTTA CTGGGGTCCC 
     1861 TGTTTGAGGA AACAGAGCCT GCTCCTGTGC AGAGTGAAGG CCCTGGAATA GAGATGCTGC 
     1921 TTTCCCACTC AGAAGATGGT AACACCCATG TCCTCCTCCA GCTTCGGCAG AGGTTTTCAG 
     1981 GCCTGGCCCG TTGCCTGGGG CTCATGCTCA GCTCTGAGTT TGGGGCTCCT GTGTCCGTTC 
     2041 CTGTGCAGGA AATCCTGGAC CTCATCTGCC GGATCCTTGG TATCAGTAGC AAGAATATTA 
     2101 ACTTGCTTGG AGATGGTCCC CTCAGGTTGC TGCTGCTGCC TTCTCTGCAC CTTGAGGCCT 
     2161 TGGACTTGCT CTCTGCCCTG ATCCTTGCGT GTGGAAGTCG ACTTTTGCGT TTTGGTGCCC 
     2221 TGATCAGCCG GCTGCTTCCC CAGGTTCTCA ATGCCTGGAG CACTGGGAGG GATACTCTAG 
     2281 CTCCAGGCCA GGAGAGGCCT TACAGCACCA TTCGGACCAA AGTGTATGCT ATCTTAGAGC 
     2341 TATGGGTGCA GGTTTGTGGG GCCTCAGCAG GTATGCTTCA GGGAGGAGCC TCTGGAGAAG 
     2401 CCTTGCTCAC CCACCTGCTC AGTGACATCT CCCCTCCAGC TGATGCTCTT AAGCTATGCA 
     2461 GTACCAGGGG AAGCTCTGAT GGAGGTTTGC AAAGTGGGAA GCCAAGTGCT CCTAAGAAGC 
     2521 TCAAGCTCGA TATGGGGGAA GCTTTGGCTC CACCCAGCCA AAGGAAAGGA GATAGGAATG 
     2581 CCAACAGTGA CGTGTGCGCA GCTGCACTGA GAGGCCTCAG TCGGACCATC CTTATGTGCG 
     2641 GGCCTCTCAT CAAGGAGGAG ACTCACAGGA GACTTCATGA TCTAGTCCTG CCCCTGGTCA 
     2701 TGAGTGTCCA GCAGGGTGAG GTCCTGGGCA GCTCTCCTTA CAACAGTTCC TGCTGTCGCC 
     2761 TCGGGCTCTA CCGCCTGCTG CTGGCTTTAT TGCTGGCACC TTCCCCTCGG TGCCCTCCTC 
     2821 CTCTTGCCTG TGCCCTGAAA GCTTTCTCTC TTGGCCAATG GGAAGATAGT CTTGAGGTCT 
     2881 CCTCTTTTTG CTCAGAAGCT CTGGTGACCT GTGCTGCTCT GACACACCCC CGAGTACCTC 
     2941 CTCTACAGAG CTCAGGCCCT GCCTGCCCCA CTCCTGCCCC CGTCCCTCCT CCTGAGGCCC 
     3001 CATCTTCATT CAGGGCCCCG GCCTTCCATC CTCCAGGCCC CATGCCCTCC ATAGGTGCTG 
     3061 TGCCATCCAC AGGCCCATTA CCCTCAGCAG GCCCCATTCC CACAGTAGGC TCTATGGCGT 
     3121 CCACAGGCCA AGTGCCCTCT CGCCCTGGAC CTCCAGCAAC AGCCAACCAT TTAGGCCTCT 
     3181 CTGTCCCAGG CCTGGTATCT GTTCCACCCA GGCTCCTTCC TGGCCCTGAA AACCACCGTG 
     3241 CAGGCTCCGG TGAGGATCCT GTCCTTGCTC CTAGTGGGAC CCCTCCGCCT AGCATACCTC 
     3301 CAGATGAAAC TTTTGGGGGG AGAGTACCTA GGCCAGCCTT TGTCCACTAC GATAAGGAGG 
     3361 AGGCATCTGA TGTAGAGATC TCCTTGGAAA GCGACTCTGA TGACAGTGTG GTGATTGTAC 
     3421 CTGAGGGGCT TCCCTCCCTG CCACCTGCAC CACCCTCAGG CACTCCTCCC CCTGCGGCTC 
     3481 CTGCCGGCCC ACCAACAGCC TCACCTCCTG TGCCAGCTAA GGAGGATTCT GAAGAACTGC 
     3541 CTGCAACCCC AGGGCCTCCA CCCCCACCCC CACCCCCACC CCCACCGGCC TCTGGTCCTG 
     3601 TGACACTTCC TCCACCCCAG CTGGTCCCTG AAGGGACTCC TGGAGGGGGA GGACCCACAG 
     3661 CCATGGAAGA AGATTTGACA GTTATTAATA TCAACAGCAG TGATGAAGAG GAGGAGGAGG 
     3721 AAGAGGAAGA GGAGGAAGAA GATGAAGATG AGGAAGAAGA AGACTTTGAG GAAGAGGAAG 
     3781 AAGATGAAGA AGAATATTTT GAAGAGGAAG AAGAGGAAGA AGAGTTTGAG GAGGAATTTG 
     3841 AGGAGGAAGA AGGTGAATTA GAAGAGGAAG AAGAAGAGGA GGAAGAAGAG TTAGATGAGG 
     3901 TAGAAGATGT GGAGTTTGGT TCAGCAGGGG AGGTGGAAGA AGGTGGACCT CCACCCCCAA 
     3961 CACTGCCACC TGCTCTGCCA CCTTCAGACT CGCCCAAAGT ACAGCCTGAG GCAGAACCTG 
     4021 AACCTGGGCT CTTATTGGAA GTTGAGGAGC CAGGGCCAGA GGAGGTGCCT GGGCCTGAGA 
     4081 CTGCTCCTAC CCTGGCCCCT GAAGTGCTCC CCTCCCAGGA GGAGGGGGAG CAGGAAGTGG 
     4141 GAAGCCCTGC AGCAGGGCCA CCTCAGGAGC TTGTTGAAGA GTCCTCTGCT CCCCCTGCCC 
     4201 TGCTGGAAGA GGGGACTGAG GGTGGAGGTG ACAAAGTGCC ACCCCCACCG GAGACACCTG 
     4261 CAGAAGAAAT GGAGACAGAA GCAGAAGTTC CAGCTCCTCA GGAAAAGGAG CAAGATGATA 
     4321 CAGCTGCCAT GCTGGCTGAT TTCATCGATT GTCCCCCTGA CGATGAGAAG CCACCACCTG 
     4381 CTACAGAGCC TGACTCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4441 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4501 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4561 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4621 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4681 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4741 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4801 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4861 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4921 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4981 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5041 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5101 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5161 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5221 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5281 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5341 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5401 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5461 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5521 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5581 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5641 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5701 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5761 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5821 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5881 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5941 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6001 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6061 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6121 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6181 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6241 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6301 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6361 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6421 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6481 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6541 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6601 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6661 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6721 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6781 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6841 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6901 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6961 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7021 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7081 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7141 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7201 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7261 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7321 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7381 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7441 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7501 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7561 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7681 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7741 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7801 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7861 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7921 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7981 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8041 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8101 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8161 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8221 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//