LOCUS       MR216079                6873 bp    DNA     circular  SYN 
DEFINITION  Ckap2 (Myc-DDK-tagged) - Mouse cytoskeleton associated protein 2 (Ckap2)
ACCESSION   NM_001004140
VERSION     NM_001004140.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3030..3121 
                     /label="Myc-DDK"  
     misc_feature    3151..3736 
                     /label="PolyA signal"  
     misc_feature    3208..3227 
                     /label="Restriction Enzyme"  
     misc_feature    4012..4684 
                     /label="ColE1"  
     misc_feature    5114..5908 
                     /label="Kan/Neo"  
     misc_feature    5943..6245 
                     /label="SV40 ori"  
     misc_feature    6331..6808 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3012..3018 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3012 
                     /label="Ckap2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGAGTCC AGGAAACGCT TCCTTGGCAG GGCTGCGAGG AATCCCTTGC 
     1081 CGGTGACCCG GGACCTGCAG CTGCCCCCGA CCAGGCGAGA CCAGCCGGCC TTCCGAGAAC 
     1141 AAAGAAAACA AAAACTCAAG GAATATCTGT TAATACGAAA AACAGTGTTT CCATACAAAC 
     1201 AAGAAAATCA GATATCCAGA GATCAGAAAA TGATAACATC TGAGGATCGG GTCCAAGAAG 
     1261 GGAAAAAAGT TGTGAAACTC AAAACAGAAG TGGCTGATAA AGAAAATATT GAAAGTACAG 
     1321 TTGAGAAAAA CTGCATTCCA TTAAAAGCTG GAGAAGTAAC CAGCTCAGAA ATACATAATT 
     1381 CAAAGGATAA TGTTCAAGCT GTGCAGTTAT TATCTACAAG AGATGATTTG CCAGGTCAGA 
     1441 CTGTGACATT AGACCCGGCA TGTCACCATA AAGACAATAA AAAGATGCAA ATGACTGCAG 
     1501 AGAAGCCCAA ACAAGACAGT AATGTGTCTA AAAAGCGAGT GCTTGGCTAT TATCATGGCC 
     1561 AGATTGTTCA GTCTAAGATT AATTCATTTA GAAAGCTTCC CAGTGTCAAA GGCGAGAGTC 
     1621 TTACAACAAC CAAGAAACTT CCCACTACTG TGTCTAAAGC CATGAAGGCT CAGAGTGAGC 
     1681 CTGCGAACAC CGTCAGCGTA AAAGCCTCAA CTACAGCTGC TGCCACCAAG TTTGCAGACG 
     1741 CTAAACCTGT GAGCACTGCG TCGAAGGACA CACTTGTGCG ACCACCTATC CGAAGCCTCC 
     1801 ACAGTAGTTC CCACGGAGCT GCGAAACAGG GCCTCAGCAG GCCGCTTGCC AATGTTACAG 
     1861 TCAGAAAAGG GATGCTTGAC AAGGAGTCAC ATCGATCAGA ACCAGTTGTG TCCGTTGTCA 
     1921 AAGCCGGTTC TTCTCAGGCC CCATCAAGAA GCATAGCATC TAAAGATGCA GCCAGGACTG 
     1981 ATTCGTCTAA TACCAGACTG ATGGTAAAGC CAAAAGATAC TGACCAGCGC AGATACACGA 
     2041 TTGCAGGAGC AAGTGTTCAT AGATCAGCTC AGCTCAAGGA CACCACCGCA GAGAGGAAAG 
     2101 CTCAGATGAC GGAGTGGAGA ACTGGCAAAG GAAAGGGGCT AAAACGACCT CCTCATTCAG 
     2161 TAGTCACCCA GGCTGAGCCA AAGGGACAAA GTGAAAACCC AGTTGGGTCC TTTTGGACTA 
     2221 CCATGGCAGA AGAAGATGAG CAGAGATTAT TTACTGAAAA AGTAAACAAA ACTATTTCTG 
     2281 AATGCCTGAA CCTTATTAAT GAGGGATGCC CAAAAGAAGA AATTTTGGCT ACATTGAATG 
     2341 ACCTGATTCA TAATATTCCA GATGCCAAAA AACTTGTTAA ATATTGGATA TGTCTTGTAC 
     2401 GTATTGAACC AATCACAAGT CCTATTGAAA ATATTATCTC AATCTATGAG AAGGCCATCC 
     2461 TGGCAGGGGC TCAGCCTATT GAAGAGATGC GTCATATAAT CATAGATATT CTAACAACGA 
     2521 AGAGTCAAGA AAAAGTCAAT TTGGGAGAAA ATATTGAGGA GGCTCATGCA ACCAAGGAAC 
     2581 CTATCCAAGA AGTAAATGCT GATGCTAATG TAGGGTCAGG AAAACCAGGA GAGGAGAATG 
     2641 AACATCATGG CAAAGTTGAG GTGTATGAAG ATGATCAAGA CAACAAAATA AAAGATCCGG 
     2701 ATTTAACAAC TCCGGACTCA AAAACTGAAG CAGGTTGCAT AATTCGATAT AATGTATCTT 
     2761 CTACACCTCG GCTGCAAAGT ATGAAAAAGA TGCAACATGA TAAAAATTCC ACACTTAAAG 
     2821 AGCTAAAGTT CCTGACACCA GTGAGACGCT CACGGCGCAT TCAAGACAAG ACTTCACGAC 
     2881 TGCCAGCCAT GTTAAAAGAC CACGACCCTT CTGTGTCTTC CCTGGAGCAG TTGTCTGAGT 
     2941 TGGGAGGGGA TGCCTTTGTC TGCCGCCCTA ATGCAGCACT GTGCCCTCTG TTCTTTGAGA 
     3001 CCGACGTAGC GGAAGAGGAA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3061 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3121 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3181 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3241 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3301 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3361 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3421 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3481 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3541 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     3601 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     3661 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     3721 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     3781 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     3841 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     3901 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     3961 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4021 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4081 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4141 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4201 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4261 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4321 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4381 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4441 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4501 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4561 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     4621 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     4681 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     4741 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     4801 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     4861 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     4921 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     4981 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5041 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5101 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5161 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5221 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5281 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5341 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5401 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5461 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5521 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     5581 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     5641 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     5701 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     5761 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     5821 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     5881 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     5941 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6001 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6061 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6121 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6181 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6241 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6301 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6361 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6421 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6481 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6541 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     6601 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     6661 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     6721 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     6781 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     6841 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//