LOCUS       MR216047                7239 bp    DNA     circular  SYN 
DEFINITION  Arhgap10 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 10 (Arhgap10)
ACCESSION   NM_030113
VERSION     NM_030113.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3396..3487 
                     /label="Myc-DDK"  
     misc_feature    3517..4102 
                     /label="PolyA signal"  
     misc_feature    3574..3593 
                     /label="Restriction Enzyme"  
     misc_feature    4378..5050 
                     /label="ColE1"  
     misc_feature    5480..6274 
                     /label="Kan/Neo"  
     misc_feature    6309..6611 
                     /label="SV40 ori"  
     misc_feature    6697..7174 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3378..3384 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3378 
                     /label="Arhgap10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCTGCAG CCCCTGGAGT TTAGCGACTG CTACCTCGAC AGCCCGTGGT 
     1081 TCCGGGAGAG GATCCGCGCG CACGAAGCGG AGCTCGAGAG GACCAACAAG TTCATCAAAG 
     1141 AGCTCATCAA AGACGGCAAG AACCTCATCT CTGCCACCAA AAGTCTGTCA GCTGCCCAGA 
     1201 GGAAGTTCGC TCACTCGCTC AGAGACTTCA AGTTCGAGTT TATCGGCGAT GCTGAGACTG 
     1261 ACGATGAACG GTGCATAGAT GCCTCCTTAC GGGAATTTTC TAACTTTCTG AAGAATCTGG 
     1321 AAGAACAGAG AGAAATCATG GCATTAAGTG TGACTGAAAC CCTGATTAAA CCCTTGGAGA 
     1381 AATTCAGAAA AGAGCAGCTT GGAGCTGTAA AGGAAGAAAA AAAGAAGTTT GACAAAGAGA 
     1441 CGGAAAAGAA TTACAGTCTC ATCGACAAGC ACTTGACGCT GTCTGCCAGG AAGAAGGACT 
     1501 CACACTTGCA GGAGGCCGAC CTCCAAGTGG AGCAGAACCG GCAGCATTTC TACGAGCTCT 
     1561 CGCTTGAATA TGTGTGCAAG CTCCAGGAAA TCCAAGAAAG AAAGAAGTTC GAGTTTGTGG 
     1621 AGCCTATGCT GTCCTTCTTT CAGGGAATGT TCACTTTCTA TCATCAAGGC CACGAGCTTT 
     1681 CCAAAGACTT CAATCACTAC AAGATGGAAC TACAAATCAA CATTCAGAAT ACACGGAATC 
     1741 GATTTGAGGG AACCAGATCA GAAGTGGAGG AACTTATGAA TAAAATTAGA CAAAATCCCA 
     1801 AGGACCAGAA GCGAGCCAGC CAGTTCACAG CAGAAGGCTA CCTGTACGTC CAGGAGAAAA 
     1861 GGCCTGCTCC ATTTGGGTCC AGTTGGGTCA AACACTACTG CATGTACCGG AAAACAGCCA 
     1921 AGAAATTCAA CATGATTCCA TTTGAGCACA GATCTGGGGG GAAACTGGGG GATGGCGAGG 
     1981 CTTTCTTTTT GAAAGAATGC ACCAAAAGGC ACATGGACTC CACGGACAGA AGGTTTTGCT 
     2041 TTGACATCGA AGCTGCTGAC AGGCCTGGCG TCCCCTTGAC CGTGCAGGCG TTTTCAGAAG 
     2101 AAGAGAGGAA GCAGTGGTTG GAAGCTCTGG GTGGAAAAGA AGCTCTGTTC CACACGTTTA 
     2161 ACAGAGCCAT TGTCCCGAGA CCGGAAGGAG GTGCACAGCT GGATAAGATG GGATTCACAA 
     2221 TTCTCAGAAA ATGCATCAGC GCCGTTGAAA CCCGAGGTAT AAATGACCAA GGACTGTACA 
     2281 GAGTTGTGGG GGTGAGTTCA AAGGTCCAGA GACTTCTGAG TATGTTGATG GATGTAAAAA 
     2341 TGTGCAATGA GCTGGACCTG GAGAATTCTG CAGATTGGGA AGTGAAGACA GTAACAAGTG 
     2401 CCTTGAAGCA GTATCTGAGG AGTCTTCCAG AGCCTCTCAT GACCTATGAG TTACACAGAG 
     2461 ACTTCATTGT TCCAGCCAAG AGCGGCAGCC CGGAATCCCG AGTTAATGCC ATCCACTTCT 
     2521 TGGTACACAA ACTGCCAGAG AAGAATAAGG AGATGTTGGA TATCTTGGTG AAGCATCTAA 
     2581 CCAACGTGTC AAGTCACTCG AAGCAGAATC TAATGACAGT GGCGAATTTA GGGGTGGTGT 
     2641 TTGGACCAAC TCTGATGAGA CCACAGGAAG AGACTGTTGC CGCCATCATG GATTTGAAGT 
     2701 TTCAGAATAT CGTTGTAGAA ATCTTAATTG AAAACCATGA AAAGATTTTT CGAACCTCGC 
     2761 CTGATACCAC ATTTGCTGAG CCCACATGCC TGTCAGCATC ACCCCCAAAC GCTCCACCAC 
     2821 GACAGTCCAA GAGACAAGGG CAGAGGACGA AGAGACCAGT GGCCGTGTAC AACCTTTGTC 
     2881 TTGAGCTGGA GGAGGGTGAC AGTCCCAGCC CTCTCAAGGA GGACCCCCCC AGCAGCAGTC 
     2941 AGGACTCTCT CTCCACCCCA TCTCCCACAA CGTCAGCTGC CCATGGGCCT CCTGGACTAG 
     3001 ACGGAAACCA CCTAGCAGCA GACGGAGGGA GCTGTGGAGA TGCCACCGCC ACCACGCCAA 
     3061 GCCAGACCCG GCCATCCATG GTGCAGTGGC TAAATATGCA GTCTCCAACC ACACCAAGCT 
     3121 CCAACCCAGC TGGAACACCT CCTTCACCCA GAATGTCACC GTTCCCCCTC TCTCCTGCTG 
     3181 CCTCCATTGT AGACAAGCTG CCTGAGTGCG TTATCAATCG AAAGGCTCGG GCAGTGTACC 
     3241 CGTGTGAAGC AGAACACAGC TCGGAATTGT CATTTGAAAT AGGAGCCATT TTTGAGGATG 
     3301 TTCAGACCTC AAGGGAACCC GGATGGCTGG AAGGGACCCT GAACGGCAAG CGAGGGCTGA 
     3361 TTCCACAGAA TTATGTCAAG CTCCTGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3421 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3481 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3541 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3601 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3661 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3721 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3781 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3841 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3901 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3961 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4021 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4081 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4141 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4201 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4261 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4321 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4381 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4441 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4501 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4561 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4621 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4681 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4741 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4801 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4861 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4921 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4981 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5041 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5101 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5161 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5221 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5281 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5341 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5401 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5461 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5521 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5581 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5641 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5701 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5761 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5821 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5881 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5941 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6001 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6061 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6121 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6181 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6241 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6301 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6361 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6421 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6481 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6541 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6601 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6661 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6721 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6781 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6841 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6901 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6961 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7021 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7081 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7141 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7201 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//