LOCUS       MR216024                7260 bp    DNA     circular  SYN 
DEFINITION  Vwa5a (Myc-DDK-tagged) - Mouse von Willebrand factor A domain containing 5A (Vwa5a)
ACCESSION   NM_172767
VERSION     NM_172767.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3417..3508 
                     /label="Myc-DDK"  
     misc_feature    3538..4123 
                     /label="PolyA signal"  
     misc_feature    3595..3614 
                     /label="Restriction Enzyme"  
     misc_feature    4399..5071 
                     /label="ColE1"  
     misc_feature    5501..6295 
                     /label="Kan/Neo"  
     misc_feature    6330..6632 
                     /label="SV40 ori"  
     misc_feature    6718..7195 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3399..3405 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3399 
                     /label="Vwa5a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCATCAC TGTGGTCTAA TCACTAGCAA CAAAGAGACA GTACCACTGA 
     1081 AAAATATCTC AGTAACATTG TCCATCAATG AATTTGTGGC TGCTGTGGTG GCAACCTTAA 
     1141 ATTACGAGAA TGAGGAGAAA GTCCCATTAG AGGCGACCTT TGTGTTTCCT ATGGATGAAG 
     1201 ACTCTGCTGT CTACAGCTTT GAAGCCTTGG TGGATGGGAA GAAAATTGTG GCAGAGTTGC 
     1261 AGGATAAGAT GAAGGCTCAC AGCGAGTATG AGGAAGCCCT CTCCCAGGGC CACCAAGCAT 
     1321 ACTTATTGGA AGAGGATGAC TATTCCAGAG ATGTCTTTTC TTGCAACGTG GGGAACCTGC 
     1381 AGCCTGGAGC TAAGGTTGCA GTTACCCTGA GGTATGTGCA GGAACTGCCC TTGGAAACAG 
     1441 ATGGGGCCCT GCGCTACCTG CTCCCAGCAA TCTTGAATCC AAGATATCAG CTCTCTGAAC 
     1501 AGTCAGCAAA CAGTTGCTTG AACATCCAGA AACCCACAGT CCCTTTGGAA GACCTGCCTT 
     1561 ACACACTCAA CATGACTGCC ACCATCACTT CCCAGCATGG TATTGAAAGG GTCCAGTCCA 
     1621 ACTGCTCTTT GAGCCCTATT CAGTACCTTA CAGATGACAA GACTTCTGCT CAGGTTTCCT 
     1681 TGACTGAAGG GCACAAGTTT GACCGGGATG TGGAACTCCT GATTTACTAC AATGAAGTGC 
     1741 ACAGCCCCAG TGTAGCTGTG GAAATGGGGA TGCTGGACAT GAAGCCAGAC AGTTTGATGG 
     1801 GAGCTCCTTC TGCAATGGTG AGTTTCTACC CAGATATCCC AGAAGTGGAA GCCTCAAAGG 
     1861 CCTGTGGAGA ATTTGTGTTT CTCATGGACC GTTCAGGAAG TATGGACTCC CCCATGAGCA 
     1921 CAGAGAACAA TTCTCAGCTA CGCATCGAGG CTGCCAAGGA AACTCTGTTG CTGTTGCTGA 
     1981 AGAGTTTGCC TATGGGTTGT TATTTTAATA TCTATGGATT TGGATCTTCC TATGAGAAAT 
     2041 TCTTTCCGGA GAGTGTGAAG TACACTCAGG ATACAATGGA GGACGCAGTG AAAAGAGTGA 
     2101 AGGCTTTAAA AGCCAACTTA GGGGGTACTG AAATCTTGAC ACCCCTCTGC AACATTTACA 
     2161 AGGCATCCTC CATTCCTGGT CATCCCCTAC AGCTCTTTGT CTTCACGGAT GGAGAAGTGA 
     2221 GTGACACATT TAGTGTCATT AGAGAAGTTA AGTTAAACAG CAAGAAACAC AGATGTTTCT 
     2281 CTTTTGGAAT TGGACAAGGA GCCTCGACCA GTTTAATCAA AAATATCGCC CGGGTATCAG 
     2341 GGGGTACTGC AGTGTTTATC ACAGGCAAGG ACAGGATGCA GACCAAGGCT CTTGGGTCTC 
     2401 TCAAGTTTGC TCTACAGTGT GCAGTGGATA ACATCTCTCT AAGTTGGGAT TTGCCTCCTG 
     2461 GTTTGTCTGT TAAAATGCTT TCTCCAGAGC AACTTACCAT CTTTAGGGGT CAGAGGTTAA 
     2521 TCATCTATGC ACAACTGACT GGGCTTATGC CTCAAGTGGA GAGCACAGGA GCAGTGTGCC 
     2581 TCAAGCACAT CCTCCAGGGT AGGAGTCTGG AAAACAGGGT GACATTTTCC CTGCAACCCA 
     2641 AGGCAAATGA CAATTTCACC ATTCATCGGT TGGCTGCAAA GTCCTTGATT CAGACTAAGG 
     2701 ATTTTGGATC CCAGGAGGCC TCAAAAGAAG AAAAAGAAGA TGTGATGAAC ATCAGCCTTC 
     2761 AGTCTGGAGT CCTTAGCTCC TTCACAGCCT TCATTGCCAT AAACAAGGAG CTGAACAAAC 
     2821 CAGTGCAGGG GCCTCTGGCC CACAGAGTGA TTCCTAGGCC AGTGATGGCT GGATCTAGTA 
     2881 GTATGCGGTT CTACTCCAGT TTCTCTGGTG GCTTTAAAGG CCCACAGCCA AGAAGTTTAC 
     2941 GTGTACCAGC ATATGATTTA TGTTCAGCAG AAAGTGCTAA TCTTGTCCTC AAAAAATCTG 
     3001 CGTGTTCTGC TATACAGAAG AAAAAGACAA ATTCTTCTAC CAATAAAAGC AACTTGAAAA 
     3061 AGGAACACAA AGCATTTGGA GAGAATGCTG TGGTGCAGCT GATTTCTCTC CAAAAGGCAA 
     3121 ATGGCTCCTG GGAGCTAGAT GAAGATTTGA CCAAGATCCT GGGCACTAAG TCAAAAGACA 
     3181 TCAAGGCTGC AAACCCTGCC AAGCATGAGG ACCCCTCAGC CTGGTCCACA GTGCTGGCTG 
     3241 TGGTCTGGTT GCATGCCAAC GGCACAGACT TAAAGTGTGA ATGGGAGCTT CTGGAAAGGA 
     3301 AGGCTGTGGC CTGGCTTCAT GACCATGCAG GTTCCTCCAT CCCCATGCTT GTGCAAGCTG 
     3361 CCAATAGTCT CCTGAAATTA TCTGTGAATC CTGCTGTCTT TGGCGTCACG CGTACGCGGC 
     3421 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3481 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3541 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3601 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3661 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3721 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3781 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3841 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3901 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3961 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4021 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4081 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4141 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4201 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4261 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4321 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4381 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4441 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4501 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4561 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4621 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4681 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4741 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4801 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4861 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4921 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4981 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5041 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5101 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5161 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5221 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5281 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5341 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5401 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5461 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5521 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5581 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5641 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5701 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5761 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5821 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5881 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5941 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6001 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6061 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6121 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6181 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6241 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6301 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6361 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6421 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6481 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6541 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6601 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6661 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6721 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6781 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6841 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6901 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6961 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7021 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7081 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7141 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7201 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//