LOCUS       MR216018                7137 bp    DNA     circular  SYN 
DEFINITION  Folh1 (Myc-DDK-tagged) - Mouse folate hydrolase (Folh1), transcript variant 1
ACCESSION   NM_016770
VERSION     NM_016770.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3294..3385 
                     /label="Myc-DDK"  
     misc_feature    3415..4000 
                     /label="PolyA signal"  
     misc_feature    3472..3491 
                     /label="Restriction Enzyme"  
     misc_feature    4276..4948 
                     /label="ColE1"  
     misc_feature    5378..6172 
                     /label="Kan/Neo"  
     misc_feature    6207..6509 
                     /label="SV40 ori"  
     misc_feature    6595..7072 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3276..3282 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3276 
                     /label="Folh1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGAACGCA CTGCAGGACA GAGACTCCGC GGAGGTCCTG GGACACCGCC 
     1081 AGCGCTGGCT CCGTGTTGGG ACACTGGTGC TGGCTTTAAC CGGAACCTTC CTCATTGGCT 
     1141 TCCTCTTTGG GTGGTTTATA AAACCTTCCA ATGAAGCTAC TGGTAATGTT TCCCATTCTG 
     1201 GCATGAAGAA GGAGTTTTTG CATGAATTGA AGGCTGAGAA CATCAAAAAA TTTTTATACA 
     1261 ATTTCACACG GACACCACAC TTGGCAGGAA CACAAAATAA TTTTGAGCTT GCAAAGCAAA 
     1321 TTCATGACCA GTGGAAAGAA TTTGGCCTGG ATTTGGTTGA GTTATCCCAT TACGATGTCT 
     1381 TGCTGTCCTA TCCAAATAAA ACTCATCCTA ACTATATCTC AATAATTAAT GAAGATGGAA 
     1441 ATGAGATTTT CAAAACATCA TTATCTGAAC AGCCACCCCC AGGATATGAG AATATATCAG 
     1501 ATGTAGTGCC ACCATACAGT GCCTTCTCTC CACAAGGGAC ACCAGAGGGT GATCTAGTGT 
     1561 ATGTCAACTA TGCACGAACT GAAGACTTCT TTAAACTGGA ACGGGAAATG AAGATCAGTT 
     1621 GTTCTGGGAA GATTGTGATT GCCAGATATG GGAAAGTGTT CAGAGGAAAT ATGGTTAAAA 
     1681 ATGCTCAACT GGCAGGGGCA AAAGGAATGA TTCTGTACTC AGACCCTGCT GACTACTTTG 
     1741 TTCCTGCGGT GAAGTCCTAT CCAGATGGCT GGAACCTCCC TGGAGGTGGT GTCCAACGTG 
     1801 GAAATGTCTT AAATCTTAAT GGTGCAGGTG ACCCGCTCAC ACCAGGTTAC CCAGCAAATG 
     1861 AACATGCTTA TAGGCATGAG TTGACAAACG CTGTTGGCCT TCCAAGTATT CCTGTCCATC 
     1921 CTATTGGATA TGATGATGCA CAGAAACTCT TAGAACACAT GGGTGGTCCA GCACCCCCTG 
     1981 ACAGTAGCTG GAAGGGAGGA TTAAAAGTGC CTTACAACGT GGGACCTGGC TTTGCTGGAA 
     2041 ACTTTTCAAC ACAAAAGGTC AAGATGCATA TTCACTCTTA CACTAAAGTG ACAAGAATCT 
     2101 ATAATGTCAT TGGCACCCTC AAAGGAGCTC TGGAACCAGA CAGATATGTT ATTCTTGGAG 
     2161 GTCACCGAGA TGCTTGGGTA TTTGGTGGCA TTGACCCTCA GAGTGGAGCA GCTGTTGTTC 
     2221 ATGAAATTGT GCGGAGCTTT GGAACCCTGA AGAAGAAAGG ACGGAGGCCT AGAAGGACAA 
     2281 TTTTGTTTGC AAGCTGGGAT GCAGAAGAAT TTGGCCTTCT TGGTTCTACT GAGTGGGCAG 
     2341 AGGAACATTC AAGACTCCTA CAAGAGCGAG GTGTGGCTTA TATTAATGCT GATTCTTCCA 
     2401 TAGAAGGAAA TTACACTCTA AGAGTTGATT GCACACCACT GATGTACAGC TTAGTGTACA 
     2461 ACCTAACAAA AGAGCTGCAA AGCCCAGATG AAGGTTTTGA AGGAAAATCT CTTTATGACA 
     2521 GCTGGAAAGA AAAGAGTCCT TCACCTGAGT TCATTGGAAT GCCCAGAATT AGCAAGCTGG 
     2581 GGTCTGGCAA TGATTTTGAA GTGTTCTTCC AAAGACTTGG AATTGCTTCA GGCAGAGCCC 
     2641 GATATACTAA AAATTGGAAA ACTAACAAAG TCAGCAGCTA TCCTCTCTAT CACAGTGTCT 
     2701 ATGAAACATA TGAGCTGGTA GTAAAATTTT ATGACCCAAC ATTTAAATAC CACCTCACTG 
     2761 TGGCCCAGGT TCGAGGAGCG ATGGTATTTG AACTTGCCAA TTCTATAGTG CTTCCCTTTG 
     2821 ACTGCCAAAG TTATGCTGTA GCTCTGAAGA AGTATGCTGA CACTATCTAC AATATTTCAA 
     2881 TGAAACATCC ACAAGAAATG AAGGCTTACA TGATATCATT TGATTCACTG TTTTCTGCAG 
     2941 TCAATAATTT TACAGATGTT GCATCTAAGT TCAATCAGAG ACTGCAAGAG TTAGACAAAA 
     3001 GCAACCCCAT ATTACTGAGA ATTATGAATG ACCAGCTGAT GTATCTGGAA CGTGCATTCA 
     3061 TTGATCCTTT AGGCTTACCA GGAAGGCCTT TCTACAGGCA TATCATCTAT GCTCCAAGCA 
     3121 GCCACAACAA GTATGCAGGA GAATCATTCC CTGGGATTTA TGATGCCCTT TTTGATATAA 
     3181 GTAGCAAAGT CAATGCTTCT AAGGCCTGGA ACGAAGTGAA GAGACAGATT TCTATTGCAA 
     3241 CCTTTACAGT GCAAGCTGCA GCAGAGACTC TGAGGGAAGT AGCTACGCGT ACGCGGCCGC 
     3301 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3361 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3421 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3481 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     3541 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     3601 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     3661 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     3721 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     3781 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     3841 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     3901 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     3961 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4021 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4081 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4141 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4201 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4261 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4321 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4381 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4441 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4501 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     4561 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     4621 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     4681 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     4741 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     4801 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     4861 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     4921 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     4981 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5041 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5101 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5161 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5221 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5281 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5341 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5401 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5461 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     5521 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     5581 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     5641 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     5701 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     5761 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     5821 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     5881 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     5941 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6001 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6061 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6121 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6181 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6241 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6301 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6361 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6421 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6481 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     6541 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     6601 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     6661 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     6721 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     6781 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     6841 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     6901 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     6961 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7021 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7081 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//