LOCUS       MR215994                8646 bp    DNA     circular  SYN 
DEFINITION  Diap1 (Myc-DDK-tagged) - Mouse diaphanous homolog 1 (Drosophila) (Diap1). Note: ORF is codon optimized
ACCESSION   NM_007858
VERSION     NM_007858.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4803..4894 
                     /label="Myc-DDK"  
     misc_feature    4924..5509 
                     /label="PolyA signal"  
     misc_feature    4981..5000 
                     /label="Restriction Enzyme"  
     misc_feature    5785..6457 
                     /label="ColE1"  
     misc_feature    6887..7681 
                     /label="Kan/Neo"  
     misc_feature    7716..8018 
                     /label="SV40 ori"  
     misc_feature    8104..8581 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4785..4791 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4785 
                     /label="Diaph1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCATCC GGAGGAGGAC TTGGACCCGG TCGGGGAACA CGCGACAAGA 
     1081 AGAAAGGTCG GTCCCCTGAC GAGCTTCCTG CTACGGGAGG TGACGGTGGT AAGCATAAAA 
     1141 AATTCCTCGA GAGGTTCACT TCAATGCGGA TTAAGAAAGA AAAGGAAAAG CCCAACTCCG 
     1201 CTCACCGAAA CAGTTCTGCC AGTTACGGAG ACGACCCCAC CGCCCAAAGC CTGCAGGATA 
     1261 TTTCCGACGA GCAAGTGCTT GTGCTTTTCG AGCAGATGCT GGTTGACATG AACCTTAATG 
     1321 AAGAGAAGCA GCAGCCCCTG AGAGAGAAAG ACATCGTTAT TAAGAGAGAA ATGGTGAGTC 
     1381 AGTACCTGCA CACCAGCAAA GCTGGGATGA ACCAGAAGGA GAGCTCTAGA TCCGCTATGA 
     1441 TGTACATTCA AGAACTGAGA TCTGGCCTGC GCGATATGCA TCTCCTGTCT TGTCTGGAGA 
     1501 GCCTGAGAGT GAGCCTGAAC AACAACCCAG TAAGTTGGGT CCAAACATTC GGGGCAGAAG 
     1561 GTTTGGCTAG CCTGCTGGAC ATTCTCAAAC GGCTGCACGA CGAAAAGGAG GAAACATCCG 
     1621 GGAATTATGA TTCCCGCAAC CAGCACGAGA TCATAAGGTG TCTGAAGGCG TTCATGAACA 
     1681 ACAAGTTCGG CATAAAGACC ATGCTCGAAA CCGAGGAGGG CATTCTTTTG CTGGTCCGCG 
     1741 CCATGGATCC AGCCGTGCCT AACATGATGA TTGATGCTGC GAAACTTCTC TCTGCCCTGT 
     1801 GTATCCTGCC TCAGCCTGAG GATATGAACG AAAGGGTGCT CGAAGCTATG ACTGAACGGG 
     1861 CGGAAATGGA CGAGGTCGAG AGGTTCCAGC CCCTTCTGGA TGGGTTGAAG TCTGGAACTT 
     1921 CTATCGCACT CAAGGTCGGT TGTCTCCAAC TGATCAACGC GCTGATTACA CCCGCCGAGG 
     1981 AGCTGGATTT CCGCGTGCAC ATAAGATCAG AGCTGATGAG GCTCGGCCTG CATCAGGTAC 
     2041 TCCAAGAGCT GCGCGAGATC GAAAACGAAG ACATGAAGGT GCAGCTCTGT GTGTTCGATG 
     2101 AACAGGGCGA CGAGGACTTT TTTGATCTCA AGGGACGCCT GGACGACATC CGCATGGAAA 
     2161 TGGATGATTT TGGAGAAGTG TTCCAGATCA TCCTGAACAC AGTTAAAGAT TCTAAAGCCG 
     2221 AACCACATTT TTTGAGTATT CTGCAGCATC TGCTCCTGGT GAGGAACGAC TACGAAGCAC 
     2281 GACCCCAATA CTATAAGCTC ATTGAAGAAT GCGTTTCACA GATTGTCCTG CATAAGAATG 
     2341 GGACCGATCC GGATTTCAAG TGTAGGCACC TTCAGATCGA TATCGAGCGG CTCGTCGACC 
     2401 AAATGATTGA TAAGACCAAG GTGGAAAAGA GTGAGGCTAA GGCTACCGAA CTGGAGAAGA 
     2461 AGCTTGATAG TGAACTCACT GCCAGGCACG AGCTGCAGGT CGAGATGAAG AAGATGGAAA 
     2521 ATGACTTTGA ACAAAAACTG CAGGACTTGC AGGGGGAGAA GGATGCCCTC GACTCAGAAA 
     2581 AGCAACAGAT TACCGCTCAG AAACAAGATC TGGAAGCTGA GGTTTCCAAG CTTACGGGAG 
     2641 AAGTCGCAAA GCTGAGCAAG GAGCTTGAAG ATGCCAAGAA CGAGATGGCG AGCCTGTCAG 
     2701 CGGTGGTGGT CGCTCCTTCC GTGTCTTCTA GCGCAGCCGT GCCCCCTGCC CCCCCGCTTC 
     2761 CGGGAGATTC TGGGACCGTG ATACCGCCAC CCCCACCCCC CCCACCGCTG CCTGGCGGGG 
     2821 TCGTGCCCCC TTCACCACCA CTCCCTCCTG GTACATGCAT CCCACCTCCC CCTCCCCTTC 
     2881 CTGGCGGTGC GTGTATTCCC CCGCCACCTC AGTTGCCCGG TAGTGCTGCC ATCCCCCCAC 
     2941 CTCCGCCACT CCCCGGGGTC GCAAGCATTC CCCCTCCGCC TCCTCTCCCA GGCGCGACCG 
     3001 CAATTCCGCC TCCTCCTCCC CTGCCCGGTG CAACCGCAAT CCCACCTCCC CCCCCACTTC 
     3061 CTGGCGGTAC CGGCATCCCG CCCCCTCCAC CTCCTTTGCC CGGCAGTGTC GGAGTGCCTC 
     3121 CACCTCCGCC GCTGCCCGGA GGACCCGGGC TCCCTCCTCC GCCTCCGCCC TTTCCAGGGG 
     3181 CCCCAGGCAT TCCTCCTCCC CCTCCCGGGA TGGGAGTCCC CCCGCCTCCT CCATTCGGTT 
     3241 TCGGAGTGCC AGCAGCCCCC GTCCTGCCGT TTGGCCTCAC CCCCAAAAAA GTTTATAAGC 
     3301 CTGAGGTGCA GCTCAGAAGG CCAAATTGGT CTAAGTTTGT GGCGGAAGAT CTTAGCCAGG 
     3361 ACTGCTTCTG GACAAAGGTG AAGGAAGACC GATTTGAGAA TAACGAACTT TTCGCTAAAC 
     3421 TCACCCTGGC ATTCTCAGCA CAGACCAAGA CTTCTAAAGC AAAAAAGGAT CAGGAGGGGG 
     3481 GCGAGGAAAA AAAAAGTGTT CAAAAGAAGA AAGTTAAAGA GCTGAAAGTC TTGGATAGCA 
     3541 AAACTGCCCA GAACCTCTCT ATCTTCCTCG GGTCATTCCG CATGCCTTAC CAGGAGATCA 
     3601 AAAATGTCAT TCTTGAGGTG AATGAGGCAG TGCTGACGGA GAGCATGATC CAAAATCTTA 
     3661 TCAAGCAGAT GCCCGAGCCG GAGCAGCTCA AGATGCTCTC TGAGCTGAAG GAAGAATACG 
     3721 ACGACTTGGC CGAGTCTGAG CAATTCGGTG TGGTAATGGG GACCGTACCT CGCCTGCGAC 
     3781 CCCGACTTAA CGCGATCCTG TTTAAACTCC AGTTTTCAGA GCAAGTTGAG AATATCAAAC 
     3841 CTGAGATCGT GAGTGTGACT GCAGCTTGCG AGGAACTCAG GAAGTCCGAA AACTTCTCCA 
     3901 GCTTGCTGGA GCTCACCCTG CTTGTAGGAA ACTATATGAA TGCCGGAAGC CGGAACGCGG 
     3961 GCGCCTTTGG TTTTAATATC TCATTCCTCT GTAAACTGAG GGACACAAAG TCCGCAGACC 
     4021 AGAAAATGAC ACTCCTCCAC TTCCTCGCCG AACTGTGCGA AAACGACCAT CCCGAGGTTC 
     4081 TCAAATTCCC CGACGAACTC GCCCACGTCG AGAAGGCAAG CAGAGTCTCA GCTGAGAATC 
     4141 TGCAAAAGTC CCTCGACCAG ATGAAAAAGC AGATAGCTGA TGTGGAGCGC GACGTGCAGA 
     4201 ATTTCCCCGC TGCTACCGAT GAGAAGGACA AGTTTGTGGA AAAGATGACC TCCTTCGTCA 
     4261 AGGACGCCCA GGAGCAATAC AATAAGTTGA GGATGATGCA CAGCAACATG GAAACCCTGT 
     4321 ATAAGGAACT CGGCGATTAT TTTGTGTTCG ACCCCAAGAA GCTGAGTGTC GAGGAGTTTT 
     4381 TTATGGACCT CCATAATTTT AGAAATATGT TTTTGCAAGC CGTCAAGGAG AATCAGAAAC 
     4441 GCAGGGAAAC CGAAGAGAAA ATGAGACGAG CCAAGCTCGC AAAGGAGAAG GCAGAAAAGG 
     4501 AACGCCTTGA GAAGCAGCAG AAAAGGGAAC AGCTGATTGA TATGAACGCT GAAGGCGACG 
     4561 AAACCGGAGT GATGGATTCC CTGTTGGAGG CCCTCCAAAG CGGCGCCGCA TTCCGGCGAA 
     4621 AGAGGGGCCC CAGACAGGTA AACCGCAAGG CTGGCTGCGC TGTGACTTCT CTGCTTGCCA 
     4681 GCGAGTTGAC CAAAGACGAT GCCATGGCTC CCGGACCTGT GAAAGTACCC AAAAAGTCTG 
     4741 AGGGCGTGCC GACGATTCTG GAAGAAGCCA AGGAGCTGGT TGGGAGAGCC AGCACGCGTA 
     4801 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4861 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4921 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4981 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5041 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5101 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5161 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5221 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5281 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5341 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5401 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5461 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5521 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5581 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5641 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5701 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5761 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5821 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5881 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5941 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6001 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6061 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6121 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6181 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6241 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6301 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6361 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6421 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6481 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6541 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6601 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6661 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6721 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6781 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6841 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6901 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6961 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7021 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7081 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7141 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7201 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7261 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7321 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7381 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7441 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7501 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7561 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7621 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7681 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7741 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7801 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7861 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7921 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7981 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8041 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8101 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8161 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8221 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8281 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8341 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8401 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8461 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8521 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8581 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8641 AATTTT
//