LOCUS       MR215979                7533 bp    DNA     circular  SYN 
DEFINITION  Slfn5 (Myc-DDK-tagged) - Mouse schlafen 5 (Slfn5)
ACCESSION   NM_183201
VERSION     NM_183201.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3690..3781 
                     /label="Myc-DDK"  
     misc_feature    3811..4396 
                     /label="PolyA signal"  
     misc_feature    3868..3887 
                     /label="Restriction Enzyme"  
     misc_feature    4672..5344 
                     /label="ColE1"  
     misc_feature    5774..6568 
                     /label="Kan/Neo"  
     misc_feature    6603..6905 
                     /label="SV40 ori"  
     misc_feature    6991..7468 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3672..3678 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3672 
                     /label="Slfn5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTTCCTG GAGGATTTGG AACTGAACTT TGCGGAATGT ATACAAGATG 
     1081 GAGGAAAAGC GACCCTTGGG GTTAGGCAAA GGGAGGAAAT GGATACCACA CACTGCATGA 
     1141 AACAGAACGA AATCATCTCG CAAGCCGTGT GCGCTCTGCT GAATTCCGGT GGCGGAGTGG 
     1201 TCAGGGTTGA GATTGAGAAC GGAGACTATA ATTTTGAGAG GGATGGAGTT GGCTTGAATC 
     1261 TGCCACCATT GTTCAGAAAC CATTTAGACG AGATGCTGTA TGGAAAGCTC TTTTTAATAT 
     1321 ATGTGAGTTC GTGGGACGTG GCAGCCTCTC ACGTGAGGCT CGCTACCCTG TGCTCCAATT 
     1381 TGTACCACAG ATGTGGAACA TTTACTGAGG TCATGGATCC TGAAAAAGCC CTGAAATTCC 
     1441 TCAAAAGGGT CCAGGATCCT AGGATTCTTG GTGACTCTGA CTCGCTAAAT CTACAGGAAG 
     1501 CTCCTGTAGA TGACGCTCAG ATGATCTTAG CTTCTGATTT GTTTCATAGC CCGCAGCTCC 
     1561 AGTACCTGGA AAAACTCAAC TTCACAAAGT CCTCACACGT TGAATTTCAA ATGTTCTCGG 
     1621 CAGACCTTTC CCAGGGCATT AGAGAGAGAC TTCCCAAGTG TGTTTCTGCA CTTGCCAATT 
     1681 CTGAAGGGGG CTATGTGTTT TTTGGTGTGC ATGATGAGAC CCGTCATGTC ATTGGATGTG 
     1741 AAAAGGAAAA GATAAATTGT ACCAACTTGA AATCTACTAT CGATGCCTGT ATCAGGAAGA 
     1801 TGCCTGTCTA CCACTTCTGT GGACAAAACC ACAAAGTGCA ATATGAGCTT AAATTCCTTG 
     1861 AAGTGTATGA TAAGGAGGCC CTCCATGGGT ATGTCTGTGC AATCAAGGTC GAACGATTCT 
     1921 GCTGTGCAGC ATTTGCCAAA GCGCCCGATT CCTGGGAGAT AAAGGACAAT AATAAGAAGC 
     1981 CGCTAACTGC GAATGACTGG GCCTCTCGGA TGATAGAAAT CAACCCAGAC CTTTCCAGTT 
     2041 TCCCTCAGAT GATCCCCTGG AAGAGTATGT TAAACACCAC ACCCTGCAGC AAGACCGTGT 
     2101 TCACACATAA ATATTTGAAA TGTGTGGAAG ACCTGCAGAA GGATTACTTT CCAGTGTCAC 
     2161 CCAACAGGAT TACATATACT CCAGAGAGCG TCTACAAGGA CCTCTTCGCA GATTACAGAG 
     2221 GACTAAGAAA CTTAATAAAT ATGGAAATGC GCTGTTTCTC TCAAGGGATA TTGATCTTCT 
     2281 CCCACAGCTG GGCTGTAGAT CTAGGTCTTC AGAGGAGGCA AGATGTCATC TGTGACGCTC 
     2341 TTCTGATTTC CCCGAACAAC GTTCCAATCC TGTACACCAT TTGCAACAAG TGGGATCTGG 
     2401 GGAACAGGCA CTATTCCATG AAAGTTGCCC GTACCCTGAA GCAGAAGCTG GTGAACATGG 
     2461 GTGGCTACCC TGGAAGATTA GGCATCATTC CCTTGGTCTT ACCACTGGGT TCTCACCAAA 
     2521 GAGTAAGGAA TGACTTAGAA ATGCCAGTGT ACCCCGAATC CTATAACTTC ATAACCACAC 
     2581 AGCAGGTGGA AGCTCTGTTG CAATCTCTTG TGATCATCTT GTTTGGGTTC AGACCCCTGT 
     2641 TAAATGAAGA GTTAAACTTG GAGTCTGAGA CTGTGGCCCT GCTCTCAGAC CAACAGTACG 
     2701 GGTTGCTTTC AACTAACCTG AGCAAGCACA GAGAGATGTT TGTTCACGGC TTACCTGGAT 
     2761 CAGGGAAGAC CACTCTAGCT CTCATGATCG TGGGGAAGAT CAGGAATGTG TTCAACTGTC 
     2821 AAGCTGACAA CATTCTTTAC ATCTGTGAGA ACCAGTCCTT GAAGAGGTTC ATTGTGAGGA 
     2881 AAAACGTTTG CCAGGCAGTG ACACGGAAAA CCTTCATGAA AAATACCTTT GATAATGTGC 
     2941 AACATATCAT CGTGGACGAA GCTCAGAATT TCCGAACGGA GGACGGGAAC TGGTACGCAA 
     3001 AGGCCAAAGC CATCACTCAG AGAGCGAGAG ATGGCCCAGG AGTTCTCTAC ATTTTTCTGG 
     3061 ATTACTTTCA GATCAATCAC TTGTGTTGCA GTGGCCTCCC TGAGCTCCAA CACCAGAAGC 
     3121 CGTTATTGAA GCTCACCAGA ATGCTCCGCA GCGGAGACAA CATAACCAGC TACCTTCAGG 
     3181 ATATAATGCA TCAAATCAGA GAAAATCCTC CCCCAAATGT CCCCCAAGAA GCCTTGATGG 
     3241 TGGGTGAAGA ACTTGAATGG GGTCCAGATG TCACAGGCAA CTTAGAGATT ACTGATAACT 
     3301 TGAATTTGGA GCAGATGTCA GTCTATGTAG CAGAGAAATG CCAGAGTCTT TGGAGGTCTG 
     3361 GCTATTTCAC GGATGTTGCT GTTCTTTTCA CTAGAGCCAG AGATATAGAA AAATGTAGAG 
     3421 ACAAGCTTCT GCTAGCAATG AGGAGGAGGT CCATGTCTCA GCTCGCTGAG GAGCCCAGTT 
     3481 TACTAGTGCA GGTCAGGGAG GGGTTGGATA GTCTGGGTAG TCACGTCGTG TTGGAGAGTG 
     3541 TTCACCGATT TTCAGGCATG GAAAGAAGTA TTGTGTTTGG GATCATTCCA ATGGGATCTG 
     3601 AGACGGCCAT TTTCTACAAT GCTCTGCTCT GTCTGGCTTC CAGGGCAAGG ACACATCTGT 
     3661 ATATTGTAAA GGTTGTGTTT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3721 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3781 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3841 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3901 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3961 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4021 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4081 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4141 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4201 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4261 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4321 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4381 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4441 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4501 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4561 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4621 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4681 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4741 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4801 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4861 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4921 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4981 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5041 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5101 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5161 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5221 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5281 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5341 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5401 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5461 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5521 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5581 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5641 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5701 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5761 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5821 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5881 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5941 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6001 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6061 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6121 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6181 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6241 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6301 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6361 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6421 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6481 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6541 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6601 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6661 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6721 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6781 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6841 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6901 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6961 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7021 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7081 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7141 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7201 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7261 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7321 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7381 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7441 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7501 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//