LOCUS       MR215935                7662 bp    DNA     circular  SYN 
DEFINITION  Dstyk (Myc-DDK-tagged) - Mouse dual serine/threonine and tyrosine protein kinase (Dstyk)
ACCESSION   NM_172516
VERSION     NM_172516.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3819..3910 
                     /label="Myc-DDK"  
     misc_feature    3940..4525 
                     /label="PolyA signal"  
     misc_feature    3997..4016 
                     /label="Restriction Enzyme"  
     misc_feature    4801..5473 
                     /label="ColE1"  
     misc_feature    5903..6697 
                     /label="Kan/Neo"  
     misc_feature    6732..7034 
                     /label="SV40 ori"  
     misc_feature    7120..7597 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3801..3807 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3801 
                     /label="Dstyk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCCGAC GGGCAGTCAT GGGCCGGCGA GTCCGTGTCG GGCCCGGGTC 
     1081 CCGGAGGCGG CGGAATGATC CGCGAGCTGT GCCGGGGCTT CAGCCGCTAC CGCCGCTACC 
     1141 TGGGACGGCT GCGGCAGAAC CTGCGAGAGA CCCAGAAGTT CTTCCGCGAC ATCAAGTGCT 
     1201 CCCACAGCCA CAGTTGTCCC TCCTCCCCCG CGGGCGGCGG AGCGGCCGAG CTTGGCCCTG 
     1261 CCGGCGACGT CGCCGAAGCC CCGCTGCCGG CGGGCCAGCT GAGCTGCATT TCCTTCCCAC 
     1321 CCATGGAAGA GACGTATCTG CAGCAGCTTG TGGACCGCCT TCCCTGTATA CTGATCCTCG 
     1381 GCCAGGACTG CAACGCCAAG TGCCAGCTGC TGAACCTGCT GTTGGGGGTG CAGGTGCTTC 
     1441 CCACCCTCAA GCTGGACAGT GATGAGAGCT GTAAGCTTCG TCGCCTCCGC TTCACCTATG 
     1501 GGACTCGGAC TCGGGTCAGC TTGGCGCTCC CCGGTCAGTA TGAACTAGTG CACACGCTAG 
     1561 CTTCACATCA GGACAACTGG GAGACTATCC CTGAGGAGGA CCTGGAGGTT CAAGAAGACA 
     1621 GTGAGGACGC TGCTCATGTG CTAGCTGACC TCGAGGTGAC AATGCATCAT GCTCTCCTGC 
     1681 AGGAAGTGGA CATTGTGGTG GCACCGTGCC CGAGCCACCG GCCCTCAGTA GATGTTCTGA 
     1741 GTGACTTGGC AAATGACTTC TTGCCTGTGA TAACCTATGC ACTTCACAAG GATGAACTCT 
     1801 CTGAGCGGGG TGAGCAAGAG CTTCGGGAAG TTAGACAGTA TTTCTCCTTC CCCATGTTCT 
     1861 TTTTCAAAGT ACCCAAGCTG GAGATAATCA GCTCTTCCAG TGGGAGAGCA GAGAGTGAAA 
     1921 GGTCACCACT GTATGGACAG CTGGTGGACC TGGGCTACCT GAGCAGCAGT CACAGGAACT 
     1981 GTGTGCCCTC TGACCAGGAC TGTAAAGCTC AGAGCATGTT GGTGGAGCAA AGTGAGAAGC 
     2041 TGAAACAACT GAGTACATTT TCTCACCAGC TGCTACAGAA CCGCCTAGTG GATGCAGCCA 
     2101 AGGCCCTGAA TGTGGTGCAC AGCCACTGCC TTGACATCTT TATTAACCAG GCTTTTGACA 
     2161 TGCAGCGGGA CCTGCAGATT ACTCCCAAAC GCCTAGAATA TACTAGAAAA AAGGAGAATG 
     2221 AATTGTATGA ATCGTTGATG AATATTGCCA ACCGAAAGCA AGAGGAAATG AAGGATATGA 
     2281 TTGTTGAGAC ACTTAACACC ATGAAAGAGG AACTTCTGGA TGATGCTGCC AACATGGAGT 
     2341 TTAAAGATGT CATTGTCCCT GAGAATGGAG AAACAATCGG GACCAGGGAG ATCAAATCCT 
     2401 GCATCCGCCA GATCCAGGAG CTCATCATCT CCAGGCTCAA TCAGGCCGTG GCCAACAAGC 
     2461 TGATCAGCTC AGTGGACTAC CTGCGGGAAA GCTTTGTTGG GACCCTGGAG CGGTGTCTGC 
     2521 AGAGCCTTGA AAAATCACAG GATGTCTCAG TTCATATTAC CAGCAACTAT CTCAAACAGA 
     2581 TCTTAAATGC CGCTTACCAT GTTGAGGTCA CTTTTCACTC GGGATCCTCA GTTACTCGGA 
     2641 TGCTTTGGGA ACAAATCAAG CAGATCATCC AGCGTATCAC ATGGGTGAAC CCACCCACCA 
     2701 TCACTCTAGA GTGGAAGAGG AAGGTGGCCC AGGAAGCCAT CGACAGCCTC AGCGCCTCCA 
     2761 AGTTGGCCAA GAGCATTTGC AGCCAGTTCC GCACGCGGCT CAATAGTTCT CATGAGGCGT 
     2821 TTGCAGCCTC CTTGAGGCAG CTGGAAGCTG GCCATTCTGG CCGTCTGGAG AAAACTGAAG 
     2881 ATCTGTGGCT AAAGGTTCGA AAAGATCACG CCCCTCGTCT GGCCCGCCTT TCGCTAGAGA 
     2941 GCCGGTCTTT ACAGGATGTC TTGCTTCACC GTAAACCTAA ATTAGGACAG GAACTAGGCC 
     3001 GGGGCCAATA TGGTGTGGTA TACCTGTGTG ACAACTGGGG GGGACACTTC CCTTGTGCCC 
     3061 TCAAATCAGT TGTTCCTCCA GATGAGAAGC ACTGGAACGA TCTGGCATTG GAGTTTCATT 
     3121 ACATGAGGTC TCTGCCAAAG CATGAGCGGC TGGTCGATCT TCATGGCTCA GTCATTGACT 
     3181 ACAACTATGG TGGCGGCTCC AGCGTTGCTG TACTGCTTAT CATGGAGCGG CTACACCGGG 
     3241 ACCTCTACAC AGGGCTGAAG GCTGGGCTGA CCCTGGAGAC ACGTTTGCAA ATAGCTCTGG 
     3301 ATGTGGTGGA GGGAATCCGC TTTCTGCACA GCCAGGGGCT TGTTCATCGG GATATCAAAC 
     3361 TGAAAAATGT GCTGCTGGAC AAACAAAACC GTGCCAAGAT CACCGACTTA GGATTCTGTA 
     3421 AACCAGAAGC TATGATGTCA GGCAGCATTG TGGGGACACC AATCCATATG GCCCCTGAAC 
     3481 TTTTCACAGG AAAGTATGAT AACTCTGTGG ACGTCTATGC TTTTGGGATC CTTTTTTGGT 
     3541 ATATCTGCTC CGGCTCTATC AAGCTCCCTG AGGCATTTGA GAGGTGTGCT AGCAAAGACC 
     3601 ATCTCTGGAA TAATGTGCGC AGAGGGACCC GTCCAGAGCG TCTTCCTGTG TTTGATGAGG 
     3661 AGTGCTGGCA GTTGATGGAG GCGTGTTGGG ATGGTGACCC CTTGAAGAGG CCCCTCTTGG 
     3721 GCATCGTGCA GCCTATGCTC CGAAGCATTA TGGACCGGCT CTGCAAGTGC AGCTCTGAAC 
     3781 AACCAAACAG AGGACTAGAC GATTCTACTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3841 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3901 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3961 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4021 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4081 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4141 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4201 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4261 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4321 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4381 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4441 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4501 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4561 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4621 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4681 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4741 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4801 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4861 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4921 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4981 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5041 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5101 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5161 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5221 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5281 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5341 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5401 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5461 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5521 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5581 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5641 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5701 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5761 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5821 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5881 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5941 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6001 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6061 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6121 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6181 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6241 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6301 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6361 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6421 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6481 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6541 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6601 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6661 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6721 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6781 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6841 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6901 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6961 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7021 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7081 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7141 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7201 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7261 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7321 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7381 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7441 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7501 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7561 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7621 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//