LOCUS       MR215888                7473 bp    DNA     circular  SYN 
DEFINITION  Akap3 (Myc-DDK-tagged) - Mouse A kinase (PRKA) anchor protein 3 (Akap3)
ACCESSION   NM_009650
VERSION     NM_009650.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3630..3721 
                     /label="Myc-DDK"  
     misc_feature    3751..4336 
                     /label="PolyA signal"  
     misc_feature    3808..3827 
                     /label="Restriction Enzyme"  
     misc_feature    4612..5284 
                     /label="ColE1"  
     misc_feature    5714..6508 
                     /label="Kan/Neo"  
     misc_feature    6543..6845 
                     /label="SV40 ori"  
     misc_feature    6931..7408 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3612..3618 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3612 
                     /label="Akap3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGATAGG GTTGACTGGT TACAAAGTCA AAGTGGCGTT TGCAAAGTTG 
     1081 GTGTCTATTC ACCTGGAGAC AACCAACACC AAGACTGGAA AATGGACACA TCAACAGATC 
     1141 CTGTCCGAGT GCTCAGCTGG CTCCGCAAAG ACCTGGAGAA AAGTACAGCG GGCTTCCAGG 
     1201 ACTCGAGGTT CAAGCCTGGA GAGTCATCGT TTGTGGAGGA AGTGGCTTAC CCAGTGGACC 
     1261 AACGAAAAGG TTTCTGTGTT GATTATTACA ATACCACCAA CAAGGGCAGT CCAGGAAGAT 
     1321 TGCATTTTGA GATGTCTCAC AAGGAGAACC CTTCCCAGGG CCTCATTTCC CATGTTGGTA 
     1381 ATGGGGGTTC CATAGACGAA GTTTCCTTCT ATGCCAACCG CCTCACAAAC CTAGTGATCG 
     1441 CCATGGCCCG AAAGGAGATC AATGAGAAGA TCCACGGCGC TGAAAACAAA TGTGTCCATC 
     1501 AGTCATTGTA TATGGGGGAT GAGCCCACAC CCCACAAAAG CTTGAGTACA GTGGCCTCTG 
     1561 AGCTCGTGAA CGAGACAGTC ACCGCATGTT CCAAGAACAT TTCCAGTGAC AAAGCTCCCG 
     1621 GCTCTGGAGA CAGGGCCTCG GGGTCGTCAC AGGCCCCTGG TCTAAGATAC ACGAGCACTC 
     1681 TGAAGATCAA GGAGAGTACA AAGGAAGGCA AGTGTCCAGA CGACAAGCCC GGCACTAAGA 
     1741 AGTCTTTCTT CTATAAGGAA GTGTTTGAGT CCCGGAATGC AGGAGATGCC AAGGAGGGCG 
     1801 GGAGGTCCTT ACCAGGAGAT CAAAAACTGT TCAGGACCAG CCCCGACAAC AGGCCTGATG 
     1861 ACTTTTCAAA CTCTATCAGT CAAGGGATCA TGACCTACGC CAACAGCGTG GTGTCTGACA 
     1921 TGATGGTCTC CATCATGAAG ACGCTGAAGA TCCAGGTGAA GGACACAACC ATCGCCACGA 
     1981 TTCTGCTGAA GAAGGTGCTG ATGAAGCACG CAAAGGAGGT TGTCTCCGAC CTCATCGACT 
     2041 CCTTCATGAA GAACCTCCAC GGCGTCACGG GAAGCCTCAT GACTGACACA GACTTTGTCT 
     2101 CGGCCGTGAA ACGAAGTTTT TTTTCTCATG GAAGCCAAAA GGCCACAGAT ATCATGGATG 
     2161 CCATGCTGGG CAAGCTATAC AATGTGATGT TTGCCAAGAA ATTCCCTGAG AACATCCGGA 
     2221 GAGCCAGGGA CAAGTCGGAG AGTTACTCCC TTATCTCCAC GAAATCACGG GCTGGTGACC 
     2281 CAAAGCTCTC AAATTTGAAC TTTGCGATGA AGTCAGAATC AAAGCTGAAA GAAAATTTGT 
     2341 TTTCTACATG CAAACTAGAG AAAGAGAAGA CGTGTGCCGA AACTCTGGGT GAGCATATTA 
     2401 TTAAGGAGGG ACTGCACATG TGGCACAAGA GTCAGCAAAA ATCTCCTGGC TTGGAGCGTG 
     2461 CCGCAAAACT GGGTAACGCT CCACAGGAGG TCTCCTTTGA GTGCCCAGAT CCTTGTGAGG 
     2521 CAAACCCTCC TCACCAACCT CAGCCACCAG AGAATTTTGC AAATTTTATG TGTGACTCAG 
     2581 ACTCCTGGGC CAAGGACCTG ATTGTATCCG CCCTGCTTCT GATTCAGTAT CACCTGGCAC 
     2641 AGGGAGGAAA GATGGATGCT CAGAGCTTCC TGGAAGCTGC TGCCAGCACC AATTTTCCCA 
     2701 CCAACAAGCC ACCTCCTCCT TCTCCTGTAG TTCAGGATGA GTGCAAACTT AAGTCTCCTC 
     2761 CCCACAAGAT ATGTGACCAA GAACAAACAG AAAAGAAAGA TCTGATGAGT GTCATCTTCA 
     2821 ATTTTATCCG GAACTTACTC AGCGAGACCA TATTCAAGAG TAGCCGTAAC TGTGAATCCA 
     2881 ATGTGCATGA GCAGAACACT CAGGAAGAAG AGATACACCC GTGTGAAAGG CCTAAGACTC 
     2941 CATGTGAAAG GCCTATTACC CCGCCTGCCC CGAAATTCTG TGAGGATGAG GAGGCCACTG 
     3001 GTGGTGCCTT ATCTGGGCTA ACCAAGATGG TTGCCAACCA GCTAGACAAC TGTATGAATG 
     3061 GGCAGATGGT GGAGCACCTG ATGGACTCGG TGATGAAGTT ATGCCTCATT ATTGCCAAGT 
     3121 CCTGTGACTC TCCCCTGTCG GAGCTGGGAG AGGAAAAGTG TGGGGATGCC AGCCGGCCAA 
     3181 ATTCTGCCTT CCCAGATAAC TTATATGAGT GCCTACCAGT CAAGGGCACA GGGACAGCTG 
     3241 AAGCCCTCCT GCAGAACGCC TACCTCACCA TCCATAATGA ACTGAGAGGT TTGTCAGGAC 
     3301 AGCCCCCCGA GGGCTGTGAA ATCCCCAAGG TGATCGTCAG CAACCACAAT CTGGCTGACA 
     3361 CCGTTCAGAA CAAGCAACTG CAAGCTGTCC TTCAGTGGGT GGCTGCCTCA GAGCTCAATG 
     3421 TCCCTATTTT GTACTTTGCT GGTGACGATG AAGGAATCCA GGAGAAGCTG CTTCAGCTCT 
     3481 CAGCCACTGC CGTGGAGAAA GGCCGCAGCG TCGGGGAGGT TCTGCAGTCG GTGCTGAGGT 
     3541 ACGAGAAGGA GCGACAGCTG GATGAAGCAG TGGGAAATGT CACGCGGCTG CAGCTGCTGG 
     3601 ACTGGCTGAT GGCAAACCTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3661 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3721 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3781 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3841 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3901 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3961 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4021 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4081 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4141 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4201 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4261 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4321 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4381 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4441 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4501 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4561 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4621 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4681 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4741 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4801 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4861 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4921 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4981 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5041 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5101 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5161 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5221 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5281 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5341 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5401 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5461 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5521 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5581 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5641 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5701 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5761 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5821 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5881 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5941 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6001 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6061 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6121 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6181 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6241 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6301 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6361 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6421 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6481 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6541 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6601 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6661 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6721 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6781 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6841 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6901 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6961 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7021 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7081 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7141 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7201 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7261 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7321 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7381 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7441 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//