LOCUS       MR215873                8136 bp    DNA     circular  SYN 
DEFINITION  Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2
ACCESSION   NM_177786
VERSION     NM_177786.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4293..4384 
                     /label="Myc-DDK"  
     misc_feature    4414..4999 
                     /label="PolyA signal"  
     misc_feature    4471..4490 
                     /label="Restriction Enzyme"  
     misc_feature    5275..5947 
                     /label="ColE1"  
     misc_feature    6377..7171 
                     /label="Kan/Neo"  
     misc_feature    7206..7508 
                     /label="SV40 ori"  
     misc_feature    7594..8071 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4275..4281 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4275 
                     /label="Depdc5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAACCACA AAGGTCTACA AACTTGTCAT CCACAAGAAG GGCTTTGGGG 
     1081 GCAGTGATGA TGAGCTAGTC GTGAACCCTA AAGTATTTCC TCACATCAAA CTTGGAGACA 
     1141 TTGTGGAGAT TGCTCACCCC AATGATGAGT ACAGTCCTTT GCTTTTGCAA GTCAAGTCGC 
     1201 TTAAGGAAGA TTTACAGAAA GAAACTATCA GTGTGGACCA GACTGTGACT CAAGTATTCC 
     1261 GGCTAAGACC TTATCAAGAT GTCTATGTGA ATGTTGTAGA CCCCAAGGAT GTGACTCTTG 
     1321 ACCTGGTGGA ATTGACTTTT AAGGATCAGT ATATTGGCCG TGGGGATATG TGGCGACTAA 
     1381 AGAAAAGTTT GGTGAGCACC TGTGCCTATA TCACTCAAAA AGTGGAATTT GCTGGCATCA 
     1441 GGGCACAGGC TGGTGAGCTG TGGGTCAAGA ATGAGAAGGT CATGTGTGGT TACATTAGTG 
     1501 AAGAGACCAG GGTGGTGTTC CGTTCTACGT CGGCTATGGT TTACATATTT ATTCAGATGA 
     1561 GCTGTGAAAT GTGGGATTTT GATATTTATG GGGATCTGTA CTTTGAGAAA GCTGTGAACG 
     1621 GTTTCCTCGC CGACCTGTTT ACTAAATGGA AGGAGAAGAA CTGTAGTCAT GAAGTGACTG 
     1681 TGGTCCTGTT TTCCAGAACT TTCTATGATG CAAAATCTAT TGATGAATTT CCTGAAATAA 
     1741 ACCGAGCTTC AATTCAAGAG GATCACAAAG GGAGATTCTA TGAGGACTTT TACAAAGTGG 
     1801 TGGTGCAGAA TGAGAGAAGG GAAGAGTGGA CTTCACTCCT CGTGACCATT AAAAAACTCT 
     1861 TCATCCAGTA TCCAGTGTTG GTGCGACTGG AACAGGCAGG GGGCTTTCCT CAAGGAGACA 
     1921 ATTCTACCTC AGCACAAGGA AACTACCTAG AGGCCATCAA CTTGTCATTC AATGTGTTTG 
     1981 ACAAGCACTA CATCAACCGA AACTTTGACC GAACTGGGCA GATGTCTGTG GTGATCACGC 
     2041 CGGGGGTGGG TGTCTTTGAA GTGGACCGCC TACTCATGAT CTTGACCAAG CAGCGGATGA 
     2101 TTGATAATGG AATTGGTGTG GACTTAGTGT GCATGGGAGA GCAGCCACTA CATGCTGTGC 
     2161 CATTATTCAA GCTACATAAC CGGAGTGTTC CGAGGGATTC TCGACTGGGT GATGATTATA 
     2221 ATATTCCTCA CTGGATAAAC CATAGTTTCT ACACATCCAA AAGCCAGCTC TTTTGTAACA 
     2281 GTTTCACTCC ACGGATAAAA CTAGCAGGAA AGAAGTCTGC CTCTGAGAAA ACCAAAAATG 
     2341 GTCGTGATAC ATCTCTCGGG ACTCCAAAGG AATCTGAAAA CACCCTTCCC ATCCAAGTAG 
     2401 ATTATGATGC CTACGATGCT CAAGTGTTCA GGCTGCCTGG CCCATCCCGG GCTCAGCGCC 
     2461 TCGCCACCTG CAGGTCTGTG AGAGAACAGG AGAATCACAG TCGCAAAAGT GCAAGCTCCT 
     2521 GTGATGTCTC ATCCAGCCCT TCCCTGCCAA GCCGTGCACT GCCCACTGAG GAAGTGAGGA 
     2581 GCCAGGCTTC TGATGACAGC TCCCTGGGCA AGAGCACCAA CATCCTGATG ATCCCTAACC 
     2641 CCCACCTGCA CCAGTATGAA GTCAGCAGCT CACTGGGCTA CACCAGCACC CGAGATGTCC 
     2701 TGGAGAACAT GATAGAACCC CCACAGAGGG ACTCCAGTGC ACCAGGAAGG TTCCATGTGG 
     2761 GTAGTGCAGA GTCCATGCTC CATGTCCGAC CTGGAGGATA CACGCCTCAG AGAGCGCTGA 
     2821 TTAACCCCTT CGCCCCCTCG AGGATGCCCA TGAAGCTCAC CTCCAACAGA AGGCGCTGGA 
     2881 TGCACACTTT TCCTGTAGGC CCATCTGGAG AGGCCATCCA GATCCATCAT CAGACCCGGC 
     2941 AAAACATGGC AGAGCTGCAG GGCAGCAGGC AGAGGGACCC TACCCACTCC TCTGCAGAGC 
     3001 TGTTGGAGTT GGCATACCAT GAAGCTGCCG GGAGGCACAG CACTTCCCGC CAGCCTGGTG 
     3061 ACAGCATGTC CTTGAACTTC AGTGGAACGG AAGAGCTTTC TGTCAGCCTG CTTAGCAACA 
     3121 GCAGTACAGG TGTGAATCCT AGGACCCAGA ACAAGGACTC CCTAGAGGAC AGTGTTTCTA 
     3181 CCTCTCCAGA CCCAATGCCA GGCTTCTGTT GCACAGTTGG AGTGGATTGG AAGTCTCTTA 
     3241 CTACTCCTGC ATGCCTTCCC CTCACCACTG ACTACTTCCC GGACCGTCAG GGCCTGCAGA 
     3301 ATGACTACAC AGAGGGCTGC TATGATCTCC TCCCAGAAGC AGACATGGAC AGGAGGGATG 
     3361 AGGAGGGTGT TCAAATGACA GCCCAGCAAG TGTTTGAAGA GTTCATCTGC CAGCGTCTCA 
     3421 TGCAGGGTTA CCAAATCATA GTACAGCCAA AGACCCAGAA ACCCAACACC ACAGTCCCAC 
     3481 CCCCATTGAG CAGTAGCCCA CTCTATAGCA GAGGCCTTGT GTCCCGAAAT CGCCCTGAGG 
     3541 AGGAAGGCCA GTATTGGTTA AGTATGGGCA GAACTTTCCA TAAGGTGACA CTCAAGGACA 
     3601 AGATGATCAC AGTAACTCGT TACCTTCCCA AGTACCCTTA TGAATCTGCC CAGATCCATT 
     3661 ACACCTACAG CCTCTGCCCT TCCCACTCTG ACTCGGAGTT TGTCTCCTGT TGGGTGGATT 
     3721 TCTGCCACGA ACGGCTCGAG GAATACAAGT GGAATTACTT AGATCAGTAT ATTTGTTCTG 
     3781 CTGGCTCTGA AGACTTCAGC TTAATCGAGT CTCTGAAGTT CTGGAGAACC CGATTTTTAC 
     3841 TACTGCCAGC CTGTGTTACT GCCACCAAGC GCATCACAGA AGGGGAGGTG CACTGTGACA 
     3901 TCTATGGGGA CAAACCCCGT GCAGATGAAG ATGAGTGGCA GCTTCTAGAT GGCTTTATTC 
     3961 GCTTTGTAGA GGGCTTAAAT CGCATCCGCA GGCGCCACCG CTCAGACCGC ATGATTCGGA 
     4021 AGGGGACTGC CATGAAAGGC TTGCAGATGA CCGGGCCCAT CTCTGCACAC TCTCTCGAGG 
     4081 CAGCAGGCCC TCCAGTCGGC AAGAAAGGAA CCTCAGCTCT GTCTGCCCTG CTGGAGATGG 
     4141 AAGCTAGTCA GAAGAGCCTA GGAGAACAGC AGACGACTGT GCACGGAAAA AGCTCCACTC 
     4201 AGCCAGCTGA GAACAGCAGT GTTGCCATGA CTCCCACCTA TGTGGACAGC CCACGCAAGG 
     4261 TGCAGCATGT GACCCGCACA GACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4321 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4381 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4441 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4501 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4561 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4621 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4681 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4741 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4801 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4861 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4921 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4981 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5041 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5101 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5161 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5221 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5281 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5341 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5401 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5461 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5521 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5581 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5641 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5701 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5761 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5821 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5881 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5941 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6001 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6061 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6121 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6181 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6241 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6301 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6361 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6421 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6481 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6541 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6601 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6661 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6721 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6781 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6841 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6901 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6961 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7021 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7081 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7141 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7201 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7261 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7321 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7381 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7501 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7561 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7621 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7681 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7741 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7801 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7861 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7921 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7981 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8041 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8101 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//