LOCUS       MR215858                7794 bp    DNA     circular  SYN 
DEFINITION  Cse1l (Myc-DDK-tagged) - Mouse chromosome segregation 1-like (S. cerevisiae) (Cse1l)
ACCESSION   NM_023565
VERSION     NM_023565.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3951..4042 
                     /label="Myc-DDK"  
     misc_feature    4072..4657 
                     /label="PolyA signal"  
     misc_feature    4129..4148 
                     /label="Restriction Enzyme"  
     misc_feature    4933..5605 
                     /label="ColE1"  
     misc_feature    6035..6829 
                     /label="Kan/Neo"  
     misc_feature    6864..7166 
                     /label="SV40 ori"  
     misc_feature    7252..7729 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3933..3939 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3933 
                     /label="Cse1l"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCTCAGC GATGCGAATT TACAGACACT AACAGAGTAT TTAAAGAAAA 
     1081 CCCTGGATCC TGATCCTGCC ATTAGACGGC CAGCTGAGAA GTTTCTCGAA TCCGTAGAAG 
     1141 GAAATCAGAA TTACCCACTG TTGCTTTTAA CATTATTGGA AAAGTCACAG GATAATGTCA 
     1201 TTAAAGTATG TGCTTCAGTA ACCTTCAAGA ACTATATTAA AAGGAATTGG AGAATTGTTG 
     1261 AAGATGAACC AAACAAAATT TGCGAAGCTG ATCGAGTAGC TATTAAAGCC AACATAGTGC 
     1321 ATCTGATGCT GAGCAGTCCA GAGCAGATTC AGAAGCAGCT GAGTGATGCT ATTAGCATCA 
     1381 TTGGCAGAGA AGATTTCCCT CAGAAATGGC CTGACTTGTT GACAGAAATG GTGAATCGCT 
     1441 TTCAGAGTGG AGATTTTCAC GTCATTAATG GAGTCCTTCG TACAGCGCAC TCACTATTTA 
     1501 AGAGATACCG GCATGAGTTC AAGTCAAATG AGCTGTGGAC TGAAATTAAA CTTGTCTTGG 
     1561 ATGCCTTTGC TTTGCCTTTG ACTAATCTAT TCAAGGCCAC CATTGAACTG TGCAGTACCC 
     1621 ATGCAAATGA CGCCTCTGCC CTGAGGATTC TTTTTTCTTC CTTGATTTTG ATCTCAAAAT 
     1681 TGTTCTATAG TTTGAACTTT CAGGATCTCC CTGAATTCTT TGAAGATAAT ATGGAAACTT 
     1741 GGATGAACAA TTTTCATACC CTCCTGACAC TGGATAATAA GCTCTTGCAG ACTGATGATG 
     1801 AAGAGGAAGC AGGCTTACTG GAGCTTTTAA AGTCTCAGAT CTGTGATAAT GCCGCTCTCT 
     1861 ATGCACAGAA ATATGACGAA GAGTTTCAGC GGTACCTCCC TCGCTTTGTC ACAGCTATCT 
     1921 GGAACTTACT CGTTACAACA GGGCGAGAAG TTAAATATGA CTTGTTGGTT AGCAATGCAA 
     1981 TTCAGTTTCT GGCTTCAGTT TGTGAAAGGC CACATTATAA GAATCTGTTT GAGGACCAGA 
     2041 ACACGCTGAC AAGTATCTGT GAAAAAGTTA TTGTGCCTAA CATGGAATTT AGAGCTGCTG 
     2101 ATGAAGAAGC TTTTGAAGAT AATTCTGAGG AGTACATACG GAGGGATTTG GAAGGCTCTG 
     2161 ATATTGATAC TAGACGCAGG GCTGCTTGTG ATCTTGTACG AGGATTATGC AAATTTTTTG 
     2221 AAGGTCCTGT GACAGGAATC TTCTCTGGTT ATGTTAATTC CATGCTCCAG GAGTATGCAA 
     2281 AAAACCCGTC TGTCAACTGG AAACACAAAG ATGCAGCTAT TTACCTTGTC ACATCTTTGG 
     2341 CATCAAAAGC CCAGACACAG AAGCATGGAA TCACGCAAGC CAATGAACTT GTAAACCTAA 
     2401 CTGAATTTTT CGTGAATCAC ATACTTCCTG ATTTAAAATC AAATAATGTG AATGAGTTTC 
     2461 CTGTGCTTAA AGCTGATGGT ATCAAATATA TTATGATTTT TAGGAATCAA GTACCAAAAG 
     2521 AGCACCTCCT AGTCTCTATT CCCCTCTTGA TCAGTCACCT GGAGGCTGAG AGTATTGTCG 
     2581 TCCACACTTA TGCCGCACAT GCTCTCGAAA GGCTGTTCAC TATGAGAGGG TCAAACAATA 
     2641 CCACTCTTTT TACAGCTGCA GAAATCGCAC CGTTTGTTGA GATTCTGCTA ACAAACCTTT 
     2701 TCAAAGCTCT CACACTTCCT GGCTCTTCAG AAAATGAATA TATTATGAAA GCTATCATGA 
     2761 GAAGTTTCTC CCTCCTGCAA GAGGCCATCA TCCCCTATAT CCCCACTCTC ATCACGCAGC 
     2821 TCACACAGAA GCTGCTGGCT GTTAGTAAGA ATCCCAGTAA ACCTCATTTC AATCACTACA 
     2881 TGTTTGAAGC AATATGCTTG TCTATAAGAA TAACCTGCAA AGCTAACCCT GCTGCTGTTG 
     2941 TGAATTTTGA GGAGGCTCTG TTTCTAGTGT TCACTGAGAT CTTACAAAAT GATGTACAAG 
     3001 AGTTTATCCC ATATGTCTTT CAAGTGATGT CTTTACTTCT GGAAACACAT AAAAATGATA 
     3061 TCCCGTCTTC CTATATGGCC TTGTTTCCCC ACCTCCTTCA GCCAGTACTG TGGGAAAGGA 
     3121 CAGGAAACAT CCCTGCTCTA GTGAGGCTGC TCCAGGCATT CTTAGAACGA GGCTCAAGTA 
     3181 CAATAGCAAC TGCTGCAGCT GACAAAATTC CTGGATTACT GGGTGTCTTC CAGAAGCTGA 
     3241 TTGCATCCAA GGCAAACGAT CACCAAGGTT TCTATCTTCT CAACAGTATA ATCGAACACA 
     3301 TGCCTCCTGA ATCAGTTGAC CAGTATAGGA AGCAGATCTT CATTCTACTA TTCCAGAGAC 
     3361 TGCAAAACTC CAAGACCACC AAGTTTATCA AGAGTTTCTT AGTGTTTATT AACTTATATT 
     3421 GCATTAAGTA TGGGGCACTA GCATTACAAG AAATATTTGA TGGCATACAA CCAAAAATGT 
     3481 TTGGAATGGT TTTGGAAAAA ATCATTATTC CAGAGATTCA AAAGGTATCT GGGAACGTGG 
     3541 AGAAAAAAAT CTGTGCAGTT GGCATAACCA AATTACTCAC AGAGTGTCCA CCAATGATGG 
     3601 ACACGGAGTA CACTAAGCTG TGGACTCCTT TGTTACAATC TCTCATTGGC CTGTTTGAAT 
     3661 TACCTGAAGA TGACAGCATT CCTGATGAAG AGCATTTCAT TGACATAGAA GATACACCAG 
     3721 GATACCAGAC TGCCTTCTCA CAGCTGGCAT TTGCTGGTAA AAAGGAACAT GATCCTGTAG 
     3781 GTCAGATGGT AAACAACCCC AAAATCCATC TGGCACAGTC ACTTCACAAA CTGTCAACTG 
     3841 CTTGTCCTGG AAGGGTTCCC TCGATGGTGA GCACCAGCCT CAATGCAGAA GCCTTGCAGT 
     3901 ATCTCCAAGG TTACCTGCAG GCAGCCAGTG TGACCCTGTT GACGCGTACG CGGCCGCTCG 
     3961 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4021 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4081 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4141 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4201 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4261 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4321 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4381 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4441 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4501 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4561 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4621 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4681 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4741 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4801 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4861 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4921 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4981 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5041 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5101 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5161 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5221 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5281 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5341 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5401 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5461 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5521 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5581 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5641 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5701 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5761 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5821 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5881 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5941 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6001 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6061 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6121 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6181 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6241 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6301 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6361 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6421 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6481 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6541 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6601 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6661 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6721 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6781 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6841 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6901 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6961 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7021 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7081 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7141 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7201 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7261 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7321 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7381 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7441 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7501 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7561 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7621 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7681 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7741 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//