LOCUS       MR215843                9150 bp    DNA     circular  SYN 
DEFINITION  Ssh2 (Myc-DDK-tagged) - Mouse slingshot homolog 2 (Drosophila) (Ssh2)
ACCESSION   NM_177710
VERSION     NM_177710.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5307..5398 
                     /label="Myc-DDK"  
     misc_feature    5428..6013 
                     /label="PolyA signal"  
     misc_feature    5485..5504 
                     /label="Restriction Enzyme"  
     misc_feature    6289..6961 
                     /label="ColE1"  
     misc_feature    7391..8185 
                     /label="Kan/Neo"  
     misc_feature    8220..8522 
                     /label="SV40 ori"  
     misc_feature    8608..9085 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5289..5295 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5289 
                     /label="Ssh2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTTGGTC ACGGTCCAGC GGTCGCCTAC CCCCAGCACC ACCTCCAGCC 
     1081 CTTGCGCCTC GGAGGCCGAC AGTGGGGAGG AGGAATGCCG GTCACAGCCC AGAAGCATCA 
     1141 GCGAGAGCTT CTTAACTGTC AAAGGTGCTG CCCTTTTTCT ACCACGAGGG AATGGCTCGT 
     1201 CTACACCAAG AGTCAGCCAC AGACGCAACA AGCATGCAGG TGATCTCCAA CAGCATCTTC 
     1261 AAGCAATGTT CATATTACTC CGCCCAGAAG ACAACATAAG GCTGGCTGTA AGACTGGAAA 
     1321 GTACTTACCA AAACCGAACA CGCTATATGG TCGTGGTTTC AACTAATGGT AGACAAGACA 
     1381 CTGAAGAAAG CATTGTCCTG GGAATGGATT TTTCTTCTAA TGACAGTAGC ACTTGTACCA 
     1441 TGGGCTTGGT CTTGCCTCTC TGGAGTGACA CCCTAATTCA TTTGGATGGT GATGGTGGGT 
     1501 TCAGCGTATC AACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG CAAGCAATGT 
     1561 GGTCTGCACT ACAGAGTTTG CACAAGGCTT GTGAAGTGGC CAGAATGCAT AACTACTATC 
     1621 CAGGCAGCCT TTTTCTCACT TGGGTGAGTT ATTACGAGAG CCATATCAAC TCAGATCAGT 
     1681 CCTCAGTCAA TGAGTGGAAT GCTATGCAGG ATGTGCAGTC TCATCGGCCT GACTCTCCAG 
     1741 CCCTCTTCAC AGACATACCA ACTGAACGTG AGCGAACAGA AAGGCTTATT AAGACTAAAT 
     1801 TAAGGGAGAT CATGATGCAA AAGGATTTGG AGAATATCAC CTCCAAAGAG ATACGCACGG 
     1861 AGCTGGAGAT GCAGATGGTG TGCAACTTGC GAGAATTTAA GGAATTCATA GATAATGAAA 
     1921 TGATCGTGAT CCTTGGTCAG ATGGACAGTC CCACACAGAT ATTTGAGCAT GTATTCCTGG 
     1981 GCTCAGAATG GAATGCCTCG AACTTAGAGG ACTTGCAGAA CCGAGGGGTA CGGTATATCT 
     2041 TGAATGTTAC TCGAGAGATA GATAACTTCT TCCCTGGAGT CTTTGAGTAT CATAACATTC 
     2101 GAGTATATGA TGAAGAAGCA ACAGATCTCC TGGCTTACTG GAATGACACT TACAAATTCA 
     2161 TCTCTAAAGC AAAGAAACAT GGATCTAAAT GTCTTGTGCA CTGCAAAATG GGGGTGAGTC 
     2221 GCTCAGCCTC CACTGTGATT GCCTATGCAA TGAAGGAGTA TGGATGGAAT CTGGATCGAG 
     2281 CCTATGACTA TGTGAAGGAA AGACGAACGG TGACCAAGCC TAACCCCAGC TTCATGAGAC 
     2341 AACTGGAAGA ATACCAAGGG ATCTTGCTGG CCAGCAAACA GCGGCATAAC AAACTCTGGA 
     2401 GATCTCATTC AGATAGTGAC CTTTCAGACC ACCACGAACC CATCTGTAAA CCTGGGCTCG 
     2461 AGCTCAACAA GAAGGAGATG ACCACGTCAG CAGACCAGAT CGCTGAGGTG AAGACTGTGG 
     2521 AGAACCTTGC TGCCATGCCT ACCGTCTTTA TGGAACATGT GGTCCCACAA GATGCAAATC 
     2581 AGAAAGGACT ACACACCAAA GAAAGAGTCA TCTGCTTGGA GTTTTCTTCA CAAGAGTTTC 
     2641 GTGCCGGACA GATTGAAGAT GAATTAAATT TAAATGACAT CAATGGATGC TCATCAGGGT 
     2701 GTTGTCTCAG TGAATCAAAA CTCCCTCTTG ACAACTGCCA TGCGTCTAAA GCCTTACTCC 
     2761 AACCTGGACA GGCCCCAGAC ATTGCCAACA AGTTCCCAGA CTTAGCTGTG GAAGATCTGG 
     2821 AGACAGACGC ACTGAAAGCA GACATGAATG TCCACCTACT GCCAATGGAA GAGTTGACAT 
     2881 CTCGACTGAA AGACCTCCCC ATGTCACCTG ATCTGGAGTC ACCAAGCCCC CAAGCCAGTT 
     2941 GCCAAGCTGC CATCTCAGAT TTTAGTACAG ATCGTATTGA CTTTTTTAGT GCCCTGGAGA 
     3001 AATTTGTAGA GCTTTCTCAA GAAACCCGGT CTCGGTCTTT TTCTCACTCA AGGATAGAAG 
     3061 AATTAGGTGG AGGAAGGAGT GAGGGCTGTC GTTTGTCAGT GATAGAAGTG GCAGCTTCAG 
     3121 AAATGGCAGC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACGCCCCAC GCCTCTGAAG 
     3181 AGTCTTCTGT GGATGAGGAC CAGTCGAAGG CAATCACCGA ACTTGTCAGC CCAGACATCA 
     3241 TCATGCAATC TCACTCAGAA AATGCAATTT CAGTCAAAGA AATTGTGACT GAAATTGAGT 
     3301 CCATTAGTCA AGGAGTTGGG CAGGTTCAGT TAAAAGGAGA CATCCTCTCT AACCCATGCC 
     3361 ACACACCAAA GAAGAGCACC ATTCATGAGC TGCCCCTGGA GAGGGTGCCA GCCCCTGAGA 
     3421 GCAAACCTGG ACATTGGGAG CAGGATGAGA GTTTCTGTAG TGTCCAGCCA GAACTAGCTA 
     3481 GAGACTCAGG GAAGTGTGCC CCGGAGGAAG GCTGTCTGAC CACACACTCA TCCACAGCAG 
     3541 ACTTGGAAGA AGAAGAACCA GTTGAGGGGG AACATGACTG GGGCCCAGGG ATGCACTCTG 
     3601 GTGCTAAGTG GTGCCCTGGG TCTGTGAGGC GAGCCACCCT GGAGTTTGAA GAGCGCTTGC 
     3661 GACAAGAGCA AGAGAACCAT GGTACAGCCT CTGCAGGCCC CACATTATCC AATCGCAAAA 
     3721 ACTCTAAGAA TGATTCTTCT GTGGCAGACT TAATGCCAAA ATGGAAAAGT GATGAAACTA 
     3781 CCCCAGAACA TTCATTTTTC CTCAAAGAAG CCGAGCCGAG CAAGGGTAAA GGGAAATGCA 
     3841 GTGGTTCTGA AGCCGGATCA CTATCCCACT GTGAGCGTAA TCCCACCATG CCAGACTGTG 
     3901 AGCTGCTGGA GCATCATTCC TTGCCGGCTC CTCAGGACTG CCTAGGGTCA GATAGTAGAA 
     3961 GTAAGAAGCA GGAAGGAGAC CTAAAGAAGC AGAGGGCTGT GGTTCCAAAC CAGGAATGTG 
     4021 ACACACAGGC CATCCTTCTC CCCCTTCCCA AGAAAATAGA AATCATTGAG TATACGCCGA 
     4081 CAGTTACATC ACTGGGTCAC ACCGAGCCAG GAGGTGAGGC GACACCCAGC AAAGAGGGTG 
     4141 AGAAACAAGG ACTGAGGAAA GTGAAGATGG AGCAGTCTAT CACTATGTTT TGTGCGCTGG 
     4201 ATGAAAATCT GAACAGGACT CTGGAGCCCA GTCAAGTTTC TCTGCATCCC CAAGTGCTAC 
     4261 CTCTGCCTCA TTCTTCCTCA GAGTGTGACA GGCCCGCTGA CCCAAACCCT ATGTTAAGTA 
     4321 GCCCGCAAGA CAAAGGGGAC TGCCCATCCA CACCCTTCAA GACAGCAGCA CCTTTTGTCA 
     4381 GCTGCAGTAC CCAGGGAGCA TCTTTCAGCT TGGATTATTT GCTTCCCCAC TCTGTGGTTC 
     4441 ACCTGGAAGG CTGCACAGAG CAAAGCAGTG CCACTGACAA TGAACTGTCT CCAGAGCAGG 
     4501 CTAGCTGGGA GGACAGCCGG GGGCATTTCC TCTCCAGTGG TAGTGGAATG GCACACACAT 
     4561 CCTCCCCATT AACTAACGAA GACCTAAGTT TAATTAACAA ACTTGGTGAC AGTGTTGGTG 
     4621 TGTTACAGAA AAAACTGGAC CCGTCACCTG AAGCCTGTAG AATCCCACAT AGCTCTAGTA 
     4681 GTGAAAATAT AAGAGATCTC AGCCACAGCC GTGGTGTGGT GAAGGAGCAT GCTAAAGAAA 
     4741 TTGAGTCTCG AGTGATCTTC CAGGCAGGGT TTTCCAAAAC ATCCCAAATG AAGCGCTCAG 
     4801 CTTCCCTTGC CAAGTTGGGT TACCTGGACC TTTGTAAAGA CTACTTACCA GATAGAGAGC 
     4861 TTGTCTCCTC AGAATCCCCT CATCTCAAAT TGCTTCAGCC CTTCCTCAGA ACAGACTCAG 
     4921 GCATGCATGC CTTGATGGCC CATGAGCCCT CAGAAAGCGC AGGTGCCCAG CAGAACCCAC 
     4981 AGCCCACCAA GTATTCTGTA GAGCAACTCA AAACATCAGA GTGTATTGTG CAGAGCAAAC 
     5041 CAGTGGAGAG GCCCAGTGTG CAGTATGCCA AAGAGTTTGG TTACAGTCAG CAGTGTTTGC 
     5101 TCCCCAAGGC AAGACCAGAA TTGACTAGTT CTGAAGGAGG CCTTCCTTTG CTACAGACAC 
     5161 AGGGTCTGCA GTACACAGGC CCCTCTCCAG GGCTGGCTGT GGCGCCCCGT CAGCAACATG 
     5221 GCAGAACTCA CCCCCTTAGA AGACTGAAAA GAGCAAATGA TAAAAAACGG ACAACCAACC 
     5281 CCTTCTATAA CACCATGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5341 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5401 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5461 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5521 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5581 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5641 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5701 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5761 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5821 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5881 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5941 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6001 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6061 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6121 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6181 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6241 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6301 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6361 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6421 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6481 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6541 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6601 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6661 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6721 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6781 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6841 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6901 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6961 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7021 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7081 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7141 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7201 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7261 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7321 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7381 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7441 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7501 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7561 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7621 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7681 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7741 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7801 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7861 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7921 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7981 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8041 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8101 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8161 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8221 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8281 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8341 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8401 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8461 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8521 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8581 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8641 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8701 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8761 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8821 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8881 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8941 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9001 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9061 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9121 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//