LOCUS       MR215697                9297 bp    DNA     circular  SYN 
DEFINITION  Shank2 (Myc-DDK-tagged) - Mouse SH3/ankyrin domain gene 2 (Shank2), transcript variant 2
ACCESSION   NM_001113373
VERSION     NM_001113373.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5454..5545 
                     /label="Myc-DDK"  
     misc_feature    5575..6160 
                     /label="PolyA signal"  
     misc_feature    5632..5651 
                     /label="Restriction Enzyme"  
     misc_feature    6436..7108 
                     /label="ColE1"  
     misc_feature    7538..8332 
                     /label="Kan/Neo"  
     misc_feature    8367..8669 
                     /label="SV40 ori"  
     misc_feature    8755..9232 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5436..5442 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5436 
                     /label="Shank2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGTCTTTG TTAAATGCCT TCACCAAGAA GGAAGTGCCC TTCAGAGAGG 
     1081 CCCCAGCTTA TTCCAACCGC AGGCGGCGGC CCCCCAACAC ATTGGCTGCC CCCAGAGTTC 
     1141 TTCTGCGTTC TAATAGTGAC AACAACCTCA ATGCTGGTGC ACCTGAATGG GCTGTCTGCT 
     1201 CTGCAGCCAC CTCCCACCGA AGCCTCTCAC CCCAACTGCT GCAGCAGACA CCCAGCAAAC 
     1261 CTGATGGAGC CACCAAGAGC CTTGGAAGCT ATACCCCTGG GCCCCGCAGC CGTTCCCCTT 
     1321 CACTCAACAG GCTGGGTGGT ACTGCTGAGG ATGGCAAGAG GACACAGCCA CATTGGCATG 
     1381 TGGGGTCGCC CTTCACTCCT GGTGCCAACA AGGACTCCCT CTCGACCTTT GAGTACCCAG 
     1441 GGCCCAGGAG GAAGCTATAT AGTGCGGTGC CTGGGAGACT CTTCGTCGCC GTCAAGCCAT 
     1501 ACCAACCCCA AGTCGACGGC GAGATCCCCC TTCACCGAGG CGACAGGGTC AAAGTTCTGA 
     1561 GCATCGGAGA GGGCGGCTTC TGGGAAGGCA GTGCCCGTGG CCACATCGGG TGGTTCCCAG 
     1621 CTGAGTGTGT GGAAGAGGTG CAGTGTAAAC CCCGGGACAG CCAGGCAGAA ACCCGCGCGG 
     1681 ACCGCAGCAA GAAGCTCTTC CGGCATTACA CGGTGGGCTC CTATGACAGC TTTGATGCTG 
     1741 CCAGTGACTG CATTATTGAG GACAAGACGG TGGTCCTGCA AAAGAAGGAC AACGAGGGCT 
     1801 TTGGATTTGT GCTCCGAGGG GCAAAAGCCG ATACCCCCAT TGAGGAATTC ACACCCACGC 
     1861 CAGCATTTCC AGCCCTGCAG TACCTGGAGT CCGTGGATGA AGGTGGGGTG GCATGGCAAG 
     1921 CCGGACTAAG GACCGGGGAC TTCTTGATTG AGGTTAACAA TGAAAATGTC GTCAAGGTGG 
     1981 GCCACAGGCA GGTGGTGAAC ATGATCCGCC AGGGAGGGAA TCACCTCATC CTTAAGGTCG 
     2041 TCACGGTGAC CAGGAATCTA GACCCTGATG ATACAGCCAG AAAGAAAGCT CCCCCACCTC 
     2101 CAAAGCGGGC TCCGACCACG GCCCTCACCC TGCGTTCCAA GTCCATGACA GCGGAGTTGG 
     2161 AGGAACTCGT GGACAAAGCC TCAGTCCGGA AGAAGAAGGA TAAACCGGAA GAGATAGTCC 
     2221 CAGCCTCCAA GCCCTCCAGG ACTGCAGAGA ACGTGGCCAT CGAATCCAGG GTGGCGACCA 
     2281 TCAAGCAGCG GCCCACAAGC CGGTGCTTCC CAGCTGCCTC TGATGTGAAT TCCGTGTACG 
     2341 AGCGCCAAGG GATTGCTGTA ATGACGCCCA CGGTCCCTGG GAGCCCGAAA GGCCCATTTC 
     2401 TGGGCCTCCC TCGAGGTACG ATGCGAAGGC AGAAATCGAT AGACAGCAGA ATCTTTCTAT 
     2461 CAGGGATAAC AGAGGAAGAG CGGCAGTTTC TGGCTCCCCC AATGCTGAAG TTCACCCGAA 
     2521 GCTTGTCCAT GCCAGACACT TCTGAGGACA TCCCCCCTCC GCCACAGTCT GTGCCCCCCT 
     2581 CTCCCCCTCC TCCTTCCCCC ACCACATACA ACTGTCCCAG GTCCCCGACT CCAAGAGTCT 
     2641 ATGGGACAAT TAAGCCCGCG TTCAATCAGA ACCCCGTCGT CGCCAAGGTG CCCCCAGCCA 
     2701 CCAGGTCTGA CACTGTGGCC ACCATGATGC GGGAAAAGGG GATGTTCTAC AGGAGAGAGC 
     2761 TGGACAGATT TTCCCTGGAC TCAGAAGACG TCTACAGCCG CAGCCCCGCC CCACAGGCCG 
     2821 CCTTCCGCAC CAAGCGGGGA CAGATGCCTG AGAACCCGTA CTCAGAGGTG GGAAAGATAG 
     2881 CCAGCAAGGC CGTCTATGTC CCTGCCAAGC CAGCCAGGCG GAAGGGCGTG CTGGTAAAGC 
     2941 AGTCCAACGT GGAGGATAGC CCCGAGAAGA CGTGCTCCAT ACCCATCCCA ACCATCATCG 
     3001 TCAAGGAACC CTCCACCAGC AGCAGCGGCA AGAGCAGCCA GGGGAGCAGC ATGGAGATTG 
     3061 ACCCCCAGGC CACCGAGCCC GGCCAGCTGC GGCCAGATGA CAGCCTCACC GTCAGCAGCC 
     3121 CCTTTGCTGC GGCCATCGCT GGGGCTGTGC GTGACCGGGA GAAGCGTCTG GAAGCCAGGA 
     3181 GGAATTCCCC AGCCTTCCTC TCCACCGACC TGGGAGATGA GGACGTGGGT CTGGGGCCGC 
     3241 CTGCTCCCCG GATGCAGGCC TCCAAGTTCC CAGAGGAGGG TGGGTTTGGT GACGAGGATG 
     3301 AGACGGAACA ACCGCTGTTG CCTACCCCCG GGGCAGCACC CAGGGAGCTG GAGAATCACT 
     3361 TCCTAGGTGG TGGTGAGGCT GGTGCTCAGG GGGAGGCTGG GGGACCCCTG AGTTCCACAT 
     3421 CCAAAGCCAA GGGGCCTGAG AGTGGCCCAG CAGCCCCCCT CAAGAGCAGC AGCCCAGCCG 
     3481 GCCCTGAGAA TTACGTGCAC CCACTCACAG GGCGGCTGCT TGACCCCAGC TCCCCACTGG 
     3541 CCCTGGCACT CTCAGCCAGG GACCGAGCCA TGCAGGAGTC CCAGCAGGGG CACAAAGGAG 
     3601 AGGCCCCCAA GGCTGACCTT AACAAGCCTC TCTACATCGA TACCAAAATG CGGCCCAGCG 
     3661 TGGAGTCCGG CTTTCCTCCA GTCACCAGAC AGAACACCAG GGGTCCCCTG CGACGGCAAG 
     3721 AGACAGAGAA CAAGTACGAG ACAGACCTGG GCAAGGACCG GAGGGCGGAC GACAAGAAGA 
     3781 ACATGCTGAT CAACATCGTG GACACTGCCC AGCAGAAGTC AGCTGGCCTA CTGATGGTGC 
     3841 ACACAGTGGA CGTCCCCATG GCCGGGCCAC CCCTGGAGGA AGAGGAGGAC AGAGAGGATG 
     3901 GGGATACAAA GCCAGACCAC TCACCCTCCA CAGTGCCAGA AGGCGTTCCC AAAACCGAAG 
     3961 GTGCTTTACA GATCTCCGCT GCCCCGGAGC CCGCCGTCGC GCCCGGCAGG ACCATCGTGG 
     4021 CGGCGGGCTC CGTGGAAGAG GCGGTGATTC TGCCATTCCG CATCCCCCCT CCCCCTCTGG 
     4081 CATCCGTGGA CTTGGATGAG GACTTTCTTT TCACAGAACC GTTGCCTCCC CCCCTGGAAT 
     4141 TCGCCAATAG TTTTGATATC CCCGATGACC GGGCAGCTTC AGTTCCCGCT CTGGCTGACC 
     4201 TGGTCAAGCA GAAGAAAAAC GACACCCCTC AGCCCCCTAC GTTGAACTCC AGCCAACCAG 
     4261 CCAACTCCAC AGACAGTAAG AAGCCAGCCG GTATCTCGAA CTGTCTGCCC TCCTCGTTCC 
     4321 TGCCACCCCC CGAAAGTTTC GATGCAGTCA CCGACTCGGG GATTGAGGAG GTGGACAGCC 
     4381 GGAGTAGCAG CGACCACCAC CTGGAGACTA CCAGCACCAT CTCCACGGTG TCCAGCATCT 
     4441 CCACGCTGTC CTCAGAGGGC GGCGAGAGCA TGGACACCTG CACAGTCTAT GCAGACGGGC 
     4501 AAGCCTTTGT GGTTGACAAG CCCCCAGTAC CTCCAAAGCC AAAAATGAAG CCCATCGTTC 
     4561 ACAAGAGCAA CGCACTTTAC CAAGACACGC TCCCAGAAGA GGACACGGAT GGCTTTGTGA 
     4621 TCCCCCCACC TGCACCCCCG CCCCCGCCGG GCAGCGCCCA GGCCGGTGTG GCGAAGGTCA 
     4681 TCCAGCCAAG GACCTCCAAG TTGTGGGGTG ACGTTCCAGA GGTCAAAAGC CCAATTCTCT 
     4741 CAGGCCCAAA GGCAAATGTC ATTAGTGAGC TAAACTCCAT TCTGCAGCAG ATGAACAGGG 
     4801 GGAAATCGGT CAAGCCCGGG GAAGGGCTGG AGCTGCCGGT GGGAGCCAAG TCGGCCAACC 
     4861 TCGCTCCAAG AAGCCCGGAG GTCATGAGCA CCGTCTCAGG TACACGGAGC ACGACGGTCA 
     4921 CCTTCACTGT CCGCCCTGGC ACCTCCCAGC CCATCACCCT GCAGAGCCGG CCCCCCGACT 
     4981 ATGAAAGCAG AACCTCAGGA CCTAGACGCG CCCCAAGCCC TGTGGTTTCA CCAACGGAAT 
     5041 TGAGCAAAGA GATCCTGCCC ACCCCTCCCC CTCCGTCCGC CACTGCAGCC TCTCCCTCCC 
     5101 CCACACTCTC AGATGTCTTT AGCCTTCCGA GCCAGTCCCC TGCAGGGGAC CTCTTTGGCT 
     5161 TGAACCCAGC AGGACGGAGC AGGTCACCAT CTCCTTCAAT ATTGCAACAG CCAATCTCAA 
     5221 ATAAGCCTTT TACAACTAAG CCTGTCCACC TGTGGACGAA ACCAGATGTG GCAGACTGGC 
     5281 TGGAAAGTCT GAACTTGGGT GAACACAAGG AGACGTTCAT GGACAATGAG ATTGACGGCA 
     5341 GCCACCTGCC AAACCTTCAG AAGGAAGACT TGATAGATCT TGGGGTGACT CGAGTTGGGC 
     5401 ATAGGATGAA CATAGAAAGG GCTTTGAAAC AGCTGCTGGA CAGAACGCGT ACGCGGCCGC 
     5461 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5521 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5581 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5641 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5701 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5761 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5821 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5881 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5941 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6001 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6061 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6121 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6181 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6241 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6301 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6361 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6421 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6481 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6541 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6601 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6661 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6721 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6781 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6841 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6901 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6961 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7021 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7081 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7141 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7201 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7261 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7321 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7381 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7441 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7501 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7561 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7621 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7681 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7741 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7801 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7861 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7921 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7981 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8041 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8101 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8161 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8221 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8281 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8341 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8401 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8461 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8521 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8581 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8641 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8701 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8761 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8821 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8881 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8941 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9001 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9061 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9121 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9181 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9241 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//