LOCUS       MR215677                6939 bp    DNA     circular  SYN 
DEFINITION  Lcmt2 (Myc-DDK-tagged) - Mouse leucine carboxyl methyltransferase 2 (Lcmt2)
ACCESSION   NM_177846
VERSION     NM_177846.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3096..3187 
                     /label="Myc-DDK"  
     misc_feature    3217..3802 
                     /label="PolyA signal"  
     misc_feature    3274..3293 
                     /label="Restriction Enzyme"  
     misc_feature    4078..4750 
                     /label="ColE1"  
     misc_feature    5180..5974 
                     /label="Kan/Neo"  
     misc_feature    6009..6311 
                     /label="SV40 ori"  
     misc_feature    6397..6874 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3078..3084 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3078 
                     /label="Lcmt2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCCGCGA GGCCGCCAGC GCCGGGCGGG AACGGTCCAG AGCACCAACG 
     1081 ACAGCAGCTC CCTCAGCAAA CGATCGCTTG CCGCACACGG ATACGTGCGC GACCCCTTTG 
     1141 CGGCGCTGCT GGTCCCGGGA CCCGTGCGGC GCACGCCGCT TATCCACCGC GGCTATTACG 
     1201 TGCGCGCGCG CGCCGTGCGT CACTGCGTGC GCGCCTTCCT CGAACTCACT AGCGCGCTCC 
     1261 CGTCCCGGAC CAGAGCCCAG ATCCTGTCCT TGGGCTCAGG CTCCGACTCG CTTTATTTTC 
     1321 GTCTGAAAGC GGCGGGCCTC CTGGCCCGAG CTGCCGTCTG GGAGGTGGAC TTCCCGGACG 
     1381 TGTCTCGGCT CAAGGCGGAG AGGATCGAGG AGACCCCGGA GCTGCGTGCG CAGACCGGTC 
     1441 CTTTCAAGAT CGGGGACTCG GCGTCATCTC TGTGCTTCGA GAGCGCGGAC TACCGCATCC 
     1501 TGGGCGCGGA CCTGCGGGAG CTCCAGCGAT TAGGAGAGGC CCTGGATGGC GCCGGCCTGG 
     1561 ACGCCACCTC TCCCACGCTG CTCCTGGCCG AGGCGGTGCT GACCTACTTG GAGCCCTCCA 
     1621 GTGCCACAGC CCTCATTGCC TGGGCCGCCC AGCGTTTCCC CGACGCCCTT TTCGTGATCT 
     1681 ATGAGCAGAT GCAGCCGGGG GATGCCTTTG GGCAGATCAT GCTGCAGCAC TTTCAGCGGT 
     1741 TGCATTCACC CTTGCATGGC CTGGAGCTGT TTCCCGTCGT GAAGGCCCAG CGCCAGCGCT 
     1801 TCCTTCAAGC TGGCTGGACT GCCTGCAGCG CCCTGGACCT GAACGAGTTC TATCGCCGCC 
     1861 TTCTCTCGGC AGAAGAGCGC CAGCGGGTTG AGACGCTTGA ACCCTTTGAT GAATATGAGG 
     1921 AGTGGCATCT GAAGTGTTCA CATTATTTCA TCCTGGCAGC ATCTAGGGGA GACATTCTGT 
     1981 CAGAAACTCC AGTGTTTGAG CCCTCAGAGG CTTCTTTTCA GATAGATCCT GCCTCGCCTT 
     2041 CAGGGTTTCT TTCTGCCAGA GTAGTCACTA GTGACCACCA GCACTCAAGC CTGAAGCGAT 
     2101 ATGGCCACGC CTCTGCCCTC TTGAGCCCTG GCGTTATTTT CAGTGCAGGA GGCTTTGGAG 
     2161 AACAAGAGGG ACGACACTGC CGAGTGAGCA GGTTTCACGT GCTCTCCAGA TCCTGTGACT 
     2221 CGGAATGGGA AGGCTGCCAA ATAAGTACCT TGGGGACTGA AGGCCAGTGG GATGGACGCC 
     2281 TTTATCACAC CATGACAAGG CTTTCTGATA CTCGGGTTCT GGTTCTTGGA GGAAGACTCT 
     2341 CCCCAGTAAG TCCAGCCTCT GGGGCTCTCC AACTTGATTT ATACAAAAGT AAGGATAATT 
     2401 GCTCTGAGGG CCAAAATGTA ACAGTAACGA AGGCTGCCTT GGAAGAAGGT TCAGTGTTGT 
     2461 CATGTTGGCG GCATTCAACA ACGGAAGTAT ACTATCAGAA TCAAAGATAC TTATTTGTGT 
     2521 ATGGTGGCCG AAGTGTGGCT GAACCTGTAC TAAGTGACTG TCGTTTTCTA CATGTAGAGA 
     2581 CAATGGCTTG GGTCAGAATC CCAGTACAAG GCGCATCACC CGAAGGTCGG CATTCCCATA 
     2641 GCGCCTGCAG TTGGCAAGGG GGAGCACTTA TCGCTGGAGG TCTTGGGGCT TCTGAGGAAC 
     2701 TGTTGAGCTC TGTACTCTTT CTCAAACCAG TGTCCTCTGG ATTCCTCTGG GAATCAATAG 
     2761 ATATCCAGCC CTCCATTACC CCAAGGTACT CTCACACGGC TCATGTGTTT AATGGAAAGC 
     2821 TGCTTCTGGT TGGAGGGGTC TGGATTCATT CCTCCTCAGT TCCTGGAGTG ACTGTTATCT 
     2881 GTTTGACTAC AGGGTTGAGC TCTGAATATC AGATTGACAC AGCGTCTGTG CCATGGCCAT 
     2941 TAATGCTGCA CAACCACAGC AGCGCCCTCC TTCCTGAAGA GCAGCAGCTC CTGCTTATTG 
     3001 GAGGTGGAGG GAACTGCTTT TCCTTTGGTA CTTATTTCAA CCCCCACACA GTGGCATTAG 
     3061 ACCTTTCTTC CTTACGTTCA GGGCAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3121 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3181 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3241 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3301 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3361 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3421 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3481 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3541 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3601 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3661 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3721 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3781 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     3841 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     3901 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     3961 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4021 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4081 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4141 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4201 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4261 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4321 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4381 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4441 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4501 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4561 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4621 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4681 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4741 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4801 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     4861 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     4921 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     4981 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5041 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5101 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5161 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5221 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5281 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5341 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5401 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5461 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5521 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5581 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5641 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5701 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5761 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     5821 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     5881 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     5941 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6001 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6061 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6121 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6181 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6241 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6301 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6361 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6421 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6481 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6541 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6601 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6661 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6721 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6781 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     6841 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     6901 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//