LOCUS       MR215628                9390 bp    DNA     circular  SYN 
DEFINITION  Gemin5 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 1
ACCESSION   NM_001166669
VERSION     NM_001166669.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5547..5638 
                     /label="Myc-DDK"  
     misc_feature    5668..6253 
                     /label="PolyA signal"  
     misc_feature    5725..5744 
                     /label="Restriction Enzyme"  
     misc_feature    6529..7201 
                     /label="ColE1"  
     misc_feature    7631..8425 
                     /label="Kan/Neo"  
     misc_feature    8460..8762 
                     /label="SV40 ori"  
     misc_feature    8848..9325 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5529..5535 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5529 
                     /label="Gemin5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCCGGAG CCGCGGACGC TGCCGCCGTC CCCCAACTGG TACTGCTCAC 
     1081 GCTGCAGCGA CGCGGCGCCC GGAGGCATCT TTGGCTTCGC TGCGCGGACC TCCGTCTTCC 
     1141 TCGTCCGCGT GGGTCCGGGT GCGGGCGCGA GCCCAGGGGC GCCCCCGTTT CGAGTGGTAG 
     1201 GAGAGTTGGT GGGACACACT GAAAGAGTAT CTGGTTTCAC TTTTTCTCAT CACCCTGGAC 
     1261 AATACAACCT CTGTGCCACC AGCTCAGACG ATGGAACTGT GAAGGTTTGG GATGTAGAGA 
     1321 CCAAGACTGT AGTGACAGAA CACACACTCC ATCAGCATAC AATATCAGCA CTGCATTGGT 
     1381 CTCCGACAGT TAAGGACTTA ATAGTCTCTG GGGATGAAAA AGGAGTCGTT TTCTGTTACT 
     1441 GGCTTAACAG AAATGACAGC CAGCACCTCT TCACAGAGCC CAGGACCATA TTCTGTCTGA 
     1501 CGTGTTCACC TCATCACGAA AATCTAGTAG CCATTGGCTA CAAAGATGGC ATAGTGGTTA 
     1561 TCATTGACAT CAGTAAAAAG GGAGAAGTTA TTCACAGACT TCGAGGCCAC GATGACGAGA 
     1621 TCCACTCCAT AGCCTGGTGT CCTCTGTCTG GTGAAGACTG CTTATCCATC AGCCAAGAGG 
     1681 AAAACTCGGA AGAACCTGAC ATTCCCAATG GGAAGCTTAT AGCAGAAACC CCCATCACAA 
     1741 AAGGCTGCTA CTTAGCCACA GGAAGCAAAG ATCAGACCAT TCGAATCTGG AGCTGTTCCC 
     1801 GCGGCCGGGG TGTAATGGTT TTGAAATTGC CCTTCCTGAA GAGAAGAAGT GGGGGTGTGG 
     1861 ACCCAACAGT TAAAGAACGG CTTTGGTTGA CACTTCATTG GCCTAAGAAC CAACCAACAC 
     1921 AGCTGGTATC CAGTTGTTTT GGAGGTGAGC TGCTGCTATG GGACCTCACC CAGTCATGGA 
     1981 GGCGGAAGTA CACGCTCTTC AGCACCTCAG CAGAAGGGCA CAATCATTCC AGAATTGTCT 
     2041 TCAATCTGTG TTCTCTGAAG ACTGAAGATG GCAAGCAGCT GCTACTGTCC ACATCCATGG 
     2101 ATAGAGATGT AAAATGTTGG GACATGGCCA CCCTGGAGTG TTGCTGGACA TTGCCTTCTC 
     2161 TTGGAGGTTT TGCCTACAGC CTGGCCTTTT CTCCTGTGGA CGTGGGCTCC CTGGCCATAG 
     2221 GTGTTGGGGA CGGCATGATC CGGGTGTGGA ATACACTCTC CATAAAGAAC AACTACGACG 
     2281 TGAAAAACTT CTGGCAAGGA GTCAAGTCCA AGGTCACAGC GCTATGCTGG CACCCAAACA 
     2341 AGGAAGGCTG CTTAGCCTTC GGGACCGATG ACGGAAAAGT GGGATTGTAT GACACCTGCT 
     2401 CCAACAAACC TCCACAGATT TCAAGCACGT ATCATAAGAA GACCGTGTAT AGGTTGGCTT 
     2461 GGGGGCCACC AGTCCCCCCC ATGTCACTTG GAGGGGAAGG AGACAGGCCT TCCCTCACCT 
     2521 TGTACAGCTG TGGAGGGGAA GGCGTTGTGC TACAGCATAA CCCCTGGAAG CTCAGTGGAG 
     2581 AGGCCTTCGA CATCAACAAA CTCGTGAGGG ACACCAACTC CATTAGATAC AAACTGCCCG 
     2641 TGCACACAGA GATCAGCTGG AAAGGAGATG GCAAAGTCCT GGCTCTTGGG AATGAAGATG 
     2701 GATCAATAGA AATATTCCAA GTCCCCAACT TGAGGCTGCT GTGCACCATC CAGCAGCACC 
     2761 ACAAGCTTGT GAACGCCATA GTCTGGCACC ATGAGCATGG CAGCCGTCCA GAGCTGAGCT 
     2821 GTCTCCTTGC TTCTGGCTCC AACAATGCAG TCATCTACGT GCACAACCTG AAAGCAGTCC 
     2881 TAGAAAGCAA TCCTGAGTCT CCAATAACCA TCACAGAGCC CTACCGCACC CTCTCGGGGC 
     2941 ACACAGCCAA GATTACCAGT CTGGCATGGA GCCCACATCA TGACGGAAGG CTGGTATCTG 
     3001 CTTGCTACGA TGGCACAGCT CAGGTGTGGG ATGCCCTCCG GGAAGAACCT TTGTTCAATT 
     3061 TCCGAGGACA TCGAGGCCGG CTGCTATGTG TCGCGTGGTC CCCAGTGGAT CCAGAATGCA 
     3121 TCTACTCAGG TGCAGATGAC TTCTGTGTCT ACAGGTGGCT GACTTCCATG CAGGACCATT 
     3181 CCCGGCCTCC TCAAGGGAAA AAATGTATTG AACTAGAGAA AAAACGACTC TCTCAATTTA 
     3241 AGCCAAAGCT TAAAAAGAAG AAAAAACCTA CCTTGCGACT GCCTGTAAAG CAGGATTCAT 
     3301 CCGTTGGCAA TGAAGATGAG AGTGTAAAGG AGAACTCTGG ACCTGCTGAG AACGGCTTGT 
     3361 CGGACCAGGA TGGTGAGGAG GAAGCCCAGG AGCCAGAGCT GCCTCCCTCT CCTGTAGTTT 
     3421 GTGTAGAACC TGTTTCCTGT ACTGACATTT GCTCAGGCTT TGAAAAGTCA AAAGTCACTG 
     3481 TTAGTAGCAA AGCCACCTCA CTGAAAAAGG AGCCAGCTAA AGAGAAACCA GAAGCCTTGC 
     3541 TCAAGAAGAG GAAGGCTCGC TCCATGCTCC CTCTGAGCAC CAGCTTAGAC CACAGGTCCA 
     3601 AAGAGGAGCT CCATCGAGAC TGCTTGGTAC TAGCAACTGC AACACACGCC AAAGCAGAGC 
     3661 TAAATGAAGA TGTGTCTGCC GACCTTGAGG AACGATTCCA CTTGGGCCTT TTCACAGACA 
     3721 GGGCTACTCT ATACAGGATG ATGGAAACAG AAGGGAAAGG TCACTTGGAA AGTGGCCACC 
     3781 CCGAGCTTTT TCACCAGCTC ATGCTCTGGA AAGGAGATCT GAAGGGTGTT CTCCAGGCTG 
     3841 CAGCAGAGAG AGGCGAGCTG ACAGACAGTC TCGTGGCTGT GGCACCAGTG GCTGGCTACA 
     3901 GTGTATGGCT GTGGGCTGTG GAAGCCTTTG CCAAGCAGCT GTGTTTCCAG GACCAGTACG 
     3961 TCAAGGCTGC CTCTTATCTG CTATCCATCC ACAAAGTGTA CGAAGCTGTG GAACTTCTCA 
     4021 AGTCAAACCA TCTCTACAGA GAAGCTATTG CAGTTGCCAA GGCTCGGCTG CGCCCTGAGG 
     4081 ACCCTGTTCT GAAGGAACTA TACCTCAGCT GGGGGTCCAT CCTGGAGAGA GATGGCCACT 
     4141 ATGCTATTGC AGCCAAGTGC TACTTAGGAG CCACTTCCGC CTATGATGCA GCCAAAGTTC 
     4201 TGGCCAGAAA GGGAGATGCA GCCTCACTTA GAACAGCTGC AGAGCTGGCT GCCATAGCAG 
     4261 GAGAACATGA GCTGGCTGCT TCCCTGGCTC TTAGATGTGC CCAAGAGCTG CTTCTGATGA 
     4321 AGAACTGGGT GGGTGCACAA GAAGCATTGG GACTGCATGA AAGCCTGCAG GGCCAGAGAC 
     4381 TGGTCTTCTG CCTCCTTGAA CTTCTATGCC GGCACCTGGA GGAAAAACAG CCTCTAGAGG 
     4441 TCAGAGGCCC CTCTTCCATT TACCACCAGT GGGCCACAGG TTCTGAGGGA ACCTTGGTGC 
     4501 AGAGAGTAAC CGGTGTGTGG CGCAGCGCCT TCAGTGTGGA CACCCCGGAG CAGTGTCAGG 
     4561 CCGCCTTACA GAAGCTGCAG GATGTCAAGT ACCCATCAGC AACAAGTAAC ACTCCCTTCA 
     4621 GACAGCTGCT GCTTCATGTC TGCCATGACC TGACCTTGGC AATGCTGAGC CAGCAGGCTG 
     4681 CTGCCTGGGA GGAGGCAGTG CCTGCTCTGC TGCAGGCTGT GGTTCGGAGC TATACCTCAG 
     4741 GGAACTTCAC CCTCATGCAG GAAATCTACT CAGCCTTTCT CCCAGGTGGC TGTGACCACC 
     4801 TACGAGACAA ACTGGGTGAC CTCTCTCCTG CCATGGCAGC TTACAAAAGC TTAGAGGCCT 
     4861 TTTGTATTTA TGGGCAACTG TATGAAGTCT GGTGGTCGCT CTGCGGGCCT GGCCCTGAGT 
     4921 CAAGCGTCTG GGTGTTGTCA GCTGAAAGCA CGGTCTCTGA TAAACAGAGC AAGCCAGAGG 
     4981 ACAGTGCCAG TGCTGAGGAC ATGGAACAGC CTCCAGGCCC GGGCCCGAGG CTCAGTGCAG 
     5041 AGAGTGAGCG TTTGCTGAGT GCCTGCAAGG AGCTTTTCTC AGAACGGCAT GCCAGCCTGC 
     5101 AGACCTCCCA GAGGACTGTA GCCGAAGTCC AAGAGACACT GGCAGAAATG ATCCGCCAGC 
     5161 ACCAAAAGAG TCAGCTCTGC AAGGCCACAA CAAATGGTCC CAGCAGAGAT GAACCCAGCA 
     5221 GAGATGAACC CAGCCAGGAA GCAGAGCGGG CTCCTTCTCA GCCTCCGAGC CCAACCAGAG 
     5281 AAGAAAGAAA TGCACCAGTG TCTCTCCCTG AACTAACCAG AAGGCTCACA GAGGCAAATG 
     5341 AGAGGATAGC AGAGTTTCCA GAGAGTGTAA AGGCCTGGCC CTTTCCAGAT GTGCTGGAGT 
     5401 GCTGTCTCGT CTTGCTTCAC ATTGGGTCCC AGTGTCCTGA CGCTGTGGAC CCTGAGATGC 
     5461 AGCAACAGGC CCAGGAGCTC CTTCACAAGT ATGGGCACAC TCGAGCTTAC AGAAGACACT 
     5521 GCCAGAGTCG GCACACGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5581 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5641 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5701 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5761 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5821 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5881 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5941 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     6001 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     6061 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6121 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6181 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6241 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6301 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6361 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6421 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6481 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6541 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6601 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6661 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6721 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6781 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6841 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6901 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6961 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     7021 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     7081 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7141 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7201 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7261 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7321 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7381 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7441 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7501 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7561 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7621 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7681 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7741 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7801 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7861 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7921 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7981 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     8041 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     8101 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8161 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8221 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8281 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8341 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8401 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8461 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8521 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8581 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8641 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8701 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8761 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8821 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8881 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8941 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     9001 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     9061 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9121 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9181 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9241 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9301 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9361 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//