LOCUS       MR215627                8601 bp    DNA     circular  SYN 
DEFINITION  Taok2 (Myc-DDK-tagged) - Mouse TAO kinase 2 (Taok2), transcript variant 2
ACCESSION   NM_001163775
VERSION     NM_001163775.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4758..4849 
                     /label="Myc-DDK"  
     misc_feature    4879..5464 
                     /label="PolyA signal"  
     misc_feature    4936..4955 
                     /label="Restriction Enzyme"  
     misc_feature    5740..6412 
                     /label="ColE1"  
     misc_feature    6842..7636 
                     /label="Kan/Neo"  
     misc_feature    7671..7973 
                     /label="SV40 ori"  
     misc_feature    8059..8536 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4740..4746 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4740 
                     /label="Taok2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGCTGGG GGCCGGGCCG GGAGCCTGAA GGACCCTGAT GTGGCTGAGC 
     1081 TCTTCTTCAA GGATGACCCT GAGAAGCTCT TCTCTGACCT CCGGGAGATC GGCCATGGCA 
     1141 GCTTTGGAGC AGTGTACTTT GCCCGGGATG TCCGGAACAG TGAGGTGGTG GCCATCAAGA 
     1201 AGATGTCCTA TAGTGGGAAG CAATCAAATG AGAAATGGCA GGATATCATC AAGGAAGTGC 
     1261 GGTTCTTACA GAAGCTACGG CATCCTAATA CCATTCAGTA CCGGGGCTGT TACCTGAGGG 
     1321 AGCACACAGC TTGGCTGGTG ATGGAGTATT GCCTGGGCTC AGCTTCTGAT CTTCTAGAAG 
     1381 TGCACAAGAA ACCCCTGCAG GAGGTAGAGA TTGCAGCTGT GACCCATGGG GCTCTTCAGG 
     1441 GCCTGGCATA TCTACACTCA CACAACATGA TCCATAGAGA TGTGAAGGCT GGAAACATCT 
     1501 TGCTGTCAGA ACCAGGCTTG GTAAAACTGG GGGACTTTGG CTCTGCGTCA ATCATGGCAC 
     1561 CTGCCAACTC TTTTGTGGGT ACTCCATACT GGATGGCTCC AGAGGTGATC CTAGCCATGG 
     1621 ATGAGGGACA ATATGATGGC AAAGTGGATG TCTGGTCCTT GGGGATAACC TGTATTGAGC 
     1681 TAGCGGAGCG GAAGCCACCA CTGTTCAACA TGAATGCAAT GAGTGCCTTA TACCACATTG 
     1741 CACAGAATGA ATCTCCTGCT CTCCAGTCAG GACACTGGTC TGAGTACTTC CGGAATTTTG 
     1801 TTGACTCCTG TCTTCAGAAA ATCCCTCAAG ACAGACCAAC CTCAGAGGTT CTTTTGAAGC 
     1861 ACCGCTTTGT GCTCCGGGAG CGACCGCCCA CAGTCATCAT GGACCTAATC CAGAGGACCA 
     1921 AGGATGCTGT ACGGGAACTG GATAACCTGC AGTACCGAAA GATGAAGAAG ATACTGTTCC 
     1981 AAGAGGCACC CAATGGCCCT GGTGCTGAGG CCCCAGAGGA AGAGGAGCTC ACACCCTGTT 
     2041 CCCAGGAGGC AGAACCTTAC ACGCACCGTG CAGGGACACT GACCAGTCTA GAGAGCAGCC 
     2101 ATTCAGTGCC CAGCATGTCC ATCAGCGCCT CCAGCCAGAG CAGCTCAGTC AACAGCCTAG 
     2161 CAGATGCCTC AGATAATGAA GAAGAGGAGG AAGAAGAAGA GGAAGAGGAG GAGGAGGAGG 
     2221 AGGAAGAAGG CCCTGAATCC AGAGAGATGG CCATGATGCA GGAGGGGGAG CATACAGTCA 
     2281 CTTCCCACAG CTCCATCATC CACCGGCTGC CGGGCTCAGA CAACCTATAT GATGATCCCT 
     2341 ACCAGCCAGA GATGACCCCA GGTCCACTCC AGCCACCTGC AGCCCCTCCC ACCTCCACCT 
     2401 CCTCTTCTGC TCGCCGCAGA GCTTATTGCC GCAACCGAGA CCACTTTGCT ACCATTCGTA 
     2461 CTGCCTCCCT GGTCAGCCGT CAGATCCAGG AGCATGAGCA GGACTCAGCC CTGCGAGAGC 
     2521 AGCTGAGTGG CTACAAGCGG ATGCGACGTC AGCACCAGAA GCAACTGCTG GCCCTGGAGT 
     2581 CCCGTCTGAG GGGTGAACGT GAGGAGCACA GTGGGCGGTT ACAGCGGGAG CTCGAGGCAC 
     2641 AGCGGGCTGG CTTTGGGACC GAGGCTGAGA AGCTGGCCCG GAGGCACCAG GCCATTGGTG 
     2701 AGAAGGAGGC ACGGGCTGCT CAGGCGGAGG AGCGGAAGTT CCAGCAGCAC ATCCTGGGGC 
     2761 AGCAGAAGAA GGAGCTGGCC GCCCTGCTGG AGGCGCAGAA GCGAACCTAC AAGCTGCGGA 
     2821 AGGAGCAGTT GAAAGAGGAG CTCCAGGAGA ACCCTAGCAC ACCCAAACGA GAGAAGGCTG 
     2881 AGTGGCTGTT GAGGCAAAAG GAGCAGTTGC AACAGTGCCA GGCAGAAGAG GAAGCGGGGC 
     2941 TGCTGCGGAG GCAGCGCCAG TACTTTGAAC TTCAGTGTCG CCAATACAAG CGCAAGATGC 
     3001 TACTGGCTCG GCACAGCCTA GATCAGGACC TGCTTCGAGA GGACTTGAAT AAGAAACAGA 
     3061 CACAGAAGGA CTTGGAGTGT GCTCTGCTGT TACGGCAGCA TGAGGCTACC CGAGAGCTGG 
     3121 AGCTAAGACA GCTCCAGGCT GTCCAGCGCA CACGTGCTGA ACTTACCCGC CTTCAGCACC 
     3181 AGACAGAACT AGGCAACCAG CTGGAGTACA ACAAGCGACG GGAGCAAGAA TTACGGCAGA 
     3241 AGCACGCGGC CCAGGTTCGC CAGCAGCCCA AGAGCCTCAA AGTACGTGCA GGCCAGCTAC 
     3301 CCATGGGCCT CCCTGCTACT GGGGCTCTGG GACCACTCAG CACAGGCACC CCTAGTGAAG 
     3361 AGCAGCCTTG CTCATCTGGC CAGGAGGCAA TCCTGGACCA AAGGATGCTG GGAGAGGAGG 
     3421 AGGAAGCAGT GCCAGAGAGA AGGATTCTGG GAAAGGAAGG GACTACCTTG GAGCCAGAGG 
     3481 AGCAGAGGAT TCTGGGGGAA GAAATGGGAA CCTTTAGTTC CAGCCCACAA AAACATAGGA 
     3541 GTCTGGCTAA TGAGGAAGAT TGGGATATAT CTGAGGAGAT GAAGGAGATT AGAGTCCCAT 
     3601 CACTGGCATC CCAGGAGAGA AATATTATTG GCCAGGAAGA GGCAGCGGCA TGGAGTCTGT 
     3661 GGGAGAAGGA GGGTGGGAAC CTTGTGGATG TGGAGTTCAA GCTTGGCTGG GTCCAGGGTC 
     3721 CAGTTCTGAC TCCAGTCCCG GAGGAGGAAG AGGAGGAGGA AGAAGAGGGA GGGGCTCCAA 
     3781 TTGGAACCCA CAGGGACCCT GGAGATGGCT GTCCTTCCCC AGATATCCCC CCAGAGCCAC 
     3841 CTCCATCACA TCTGAGACAG TACCCTACTA GCCAGCTCCC TGGACTCCTG TCTCATGGCC 
     3901 TCCTGGCTGG CCTATCCTTT GCAGTGGGGT CCTCCTCTGG CCTCCTGCCC CTACTCCTCC 
     3961 TGCTGCTACT CCCATTGCTG GCAGCCCAGG GTGGAGGTGG CTTGCAGGCA GCACTGCTGG 
     4021 CCCTTGAGGT AGGGCTAGTG GGCCTGGGGG CCTCCTACCT GTTCCTTTGT ACAGCTCTAC 
     4081 ACCTGCCCCC CGGTCTGTTC TTACTCCTGG CTCAGGGTAC TGCACTGTTG GCTGTCCTTA 
     4141 GCCTGAGCTG GCGCAGAGGC CTTATGGGTG TGCCTCTGGG CCTTGGGGCT GCCTGGCTCC 
     4201 TAGCTTGGCC CAGCCTGGCT TTACCTCTGG CAGCTATGGC AGCTGGGGGC AAATGGGTAC 
     4261 GGCAACAAGG CCCCCAGATG CGTCGGGGTA TCTCTCGACT CTGGTTGAGG ATTTTGCTAC 
     4321 GCCTGTCACC CATGGTCTTT CGGGCCCTAC AGGGCTGTGG GGCTGTAGGA GACCGGGGGC 
     4381 TGTTTGCCCT GTACCCTAAG ACCAATAAGA ATGGTTTCCG AAGTCGACTA CCTGTCCCTT 
     4441 GGCCCCGTCA GGGAAATCCT CGCACTACTC AACACCCCCT AGCTCAGTTA ACAAGAGTTT 
     4501 GGGCTGTGTG CAAGGGCTGG AACTGGCGCC TAGCACGGGC TAGCCACAGA TTAGCTTCTT 
     4561 GCTTGCCCCC CTGGGCTGTT CATATACTAG CCAGCTGGGG CCTGCTTAAG GGTGAACGGC 
     4621 CTAGTCGGAT CCCTCGGCTG CTGCCACGCA GCCAACGCCG TCTTGGGCTC TCTGCTTCCC 
     4681 GACAGCTACC ACCAGGGACT GTAGCTGGGC GGAGATCTCA GACTCGCAGG ACCCTGCCTC 
     4741 CCTGGAGGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4801 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4861 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4921 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4981 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5041 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5101 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5161 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5221 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5281 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5341 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5401 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5461 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5521 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5581 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5641 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5701 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5761 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5821 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5881 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5941 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6001 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6061 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6121 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6181 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6241 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6301 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6361 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6421 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6481 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6541 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6601 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6661 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6721 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6781 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6841 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6901 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6961 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7021 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7081 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7141 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7201 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7261 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7321 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7381 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7441 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7501 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7561 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7621 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7681 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7741 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7801 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7861 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7921 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7981 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8041 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8101 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8161 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8221 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8281 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8341 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8401 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8461 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8521 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8581 CAAAAATTTA ACGCGAATTT T
//