LOCUS       MR215624                9315 bp    DNA     circular  SYN 
DEFINITION  Cdk12 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 2
ACCESSION   NM_001109628
VERSION     NM_001109628.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5472..5563 
                     /label="Myc-DDK"  
     misc_feature    5593..6178 
                     /label="PolyA signal"  
     misc_feature    5650..5669 
                     /label="Restriction Enzyme"  
     misc_feature    6454..7126 
                     /label="ColE1"  
     misc_feature    7556..8350 
                     /label="Kan/Neo"  
     misc_feature    8385..8687 
                     /label="SV40 ori"  
     misc_feature    8773..9250 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    5454..5460 
                     /label="Restriction Enzyme"  
     misc_feature    1029..5454 
                     /label="Cdk12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG CCCAATTCCG AGAGACATGG GGGCAAGAAG GACGGGAGCG 
     1081 GAGGAGCTTC TGGAACTTCG CAGCCGTCAT CGGGAGGTGG CAGCTCCAAC AGCAGGGAGC 
     1141 GTCACCGCTT GGTGTCGAAG CACAAGCGGC ATAAGTCCAA GCACTCCAAA GACGTGGGGC 
     1201 TGGTGACCCC CGAAGCGGCA TCTTTGGGTA CCATAATCAA ACCACTGGTG GAGTACGATG 
     1261 ACATCAGCTC TGATTCAGAC ACCTTCTCCG ATGACACGGC CTTCAAATCA GACCGGAGGG 
     1321 AGAACGAGGA ACGTCGGGGA ACGGATCGGA GCGATCGCCT GCACCGACAT CGTCACCACC 
     1381 AGCACCGGCG GTCCCGAGAC TTGCTAAAAA CTAAACAGAC GGAAAAGGAA AAAAATCAGG 
     1441 AAGTCTCCAA ATCTGGATCT ATGAAGGACC GGGTATCGGG CAGTTCCAAA CGGTCCGTGG 
     1501 AGGGGAGTGA TGATTATGGG AAGGCCCAGC TATCCAAAAG CGGCAGCAAG GAATCCAGGT 
     1561 CGTCCAAAAT GCACAAGGAG AAGACCCGCA AAGAGCGAGA GTTAAAGTCT GGATACAAGG 
     1621 ACCGGAGTAA AAGTCATCGG AAAAGGGAAA CACCCAAAAG TTACAAAACC GTGGCTAGCC 
     1681 CTAAACGGAG ATCCAGGAGT CCCCATAGGA AATGGTCTGA CAGTTCCAAG CAAGATGACA 
     1741 GCCCTTCCGG AGCTTCTTAT GGCCAAGACT ACGATCTTAG CCCCCCAAGG TCTCACACTT 
     1801 CTAGCAACTA TGACTCCTAC AAGAAGAGTC CTGGAAGTAC CTCAAGAAGG CAGTCAATCA 
     1861 GCCCACCTTA CAAAGAGCCT TCTGCTTACC AGTCCAGCAC TCGGTCACCC AGTCCTTACA 
     1921 GCCGACGACA GAGGTCTGTG AGTCCCTATA GCCGGAGACG GTCCTCCAGC TATGAAAGGA 
     1981 GCGGCTCTTA CAGCGGGAGA TCACCCAGCC CCTATGGCCG AAGGCGATCA AGCAGCCCTT 
     2041 TCCTGAGCAA GAGGTCTCTG AGTCGGAGTC CACTCCCCAG TAGGAAATCC ATGAAGTCCA 
     2101 GAAGTAGAAG TCCTGCATAT TCAAGACACT CATCTTCTCA TAGTAAAAAG AAGCGATCCG 
     2161 GGTCACGCAG TCGTCATTCC AGTATCTCAC CTGTCAGGCT TCCATTGAAT TCCAGCCTGG 
     2221 GAGCTGAACT CAGTAGAAAA AAGAAGGAAA GAGCAGCAGC TGCTGCAGCA GCAAAAATGG 
     2281 ATGGAAAAGA GTCCAAGAGT TCACCTATAA TTTTGCCTAA AAAAGAGAAA CTTGAGGTGA 
     2341 AGGAGTCAGG GTTAGAGTCT AAAAAGTTAC CCAGAAGTAT AAAATCAGAA AAATCGACCC 
     2401 CAGATACTGA ACTGGTTACT GTAGCACATT CAAACCCAGA GGTAAAACAT TGTTTAGACA 
     2461 CAGGGAAGGT TAGGTTGGAT GAGAACTTGC AGAAGCATCC TGCTAAGGAT TTGAAAGCAC 
     2521 AGGGAACAAA GGACGTTAAA CCTGTAGCAC CGAAAGAGGT GATTGTTACT TCAAAGGAGA 
     2581 CAGAGACATC AGAAAAGGAG ACCCTTCCAC CTCTTCCCAC AATTACTTCT CCACCCCCTT 
     2641 TACCAGCTAC TACCCCTCCA CCTCAGACAC CCCCTTTGCC ACCTTTGCCT CCACTACCAG 
     2701 CTATTCCGCT GCAGCCACCT CTGCCTCCTC CCCAACCACC ATTTAGTCAA GTTCCTGTTT 
     2761 CAAGTACTTC AATTTTACCC TCTTCTCCTC ACCCAAGGAC ATCTACTCTA TCCTCTCAGA 
     2821 CAAATTCTCA GCCCCCTGTA CAGGTTTCTA TGAAGACTCA AGTATCTATA ACAGCTGCTA 
     2881 TTCCACATCT GAAGACTTCA ACATTGCCTC CTCTGCCCCT CCCTCCCCTA TTACCTGGAG 
     2941 ATGATGACAT GGATAGTCCA AAAGAAACAC TTCCTTCAAA GCCTGCAAAG AAAGAGAAGG 
     3001 AACAGAGGAC TCGCCACTTG CTTACAGACT TGCCTCTTCC TCCTGAGCTA CCAGGAGGAG 
     3061 ATCCATCGCC TCCAGATTCT CCAGAGCCAA AGGCAATTAC ACCACCTCAA CAACCATATA 
     3121 AAAAGAGACC AAAAATTTGT TGTCCACGTT ATGGAGAAAG AAGACAAACA GAAAGTGATT 
     3181 GGGGGAAGCG CTGTGTGGAC AAGTTTGACA TTATTGGGAT TATTGGAGAG GGGACCTATG 
     3241 GCCAAGTATA TAAAGCCAAG GACAAAGACA CAGGAGAACT AGTAGCTCTG AAGAAGGTTC 
     3301 GGCTGGACAA TGAGAAAGAG GGCTTCCCAA TCACAGCCAT CCGGGAGATC AAGATTCTTC 
     3361 GTCAATTAGT TCACCAGAGT GTTGTAAACA TGAAGGAAAT TGTCACAGAC AAACAAGATG 
     3421 CACTGGATTT CAAGAAGGAC AAAGGTGCCT TTTACCTTGT ATTTGAATAT ATGGACCATG 
     3481 ACTTAATGGG ACTGCTTGAA TCAGGTTTGG TGCACTTTTC TGAGGACCAT ATCAAGTCAT 
     3541 TTATGAAACA GCTAATGGAA GGACTGGATT ACTGTCACAA AAAGAATTTC CTCCATCGGG 
     3601 ATATTAAATG TTCTAACATT TTGCTGAATA ACAGCGGGCA AATCAAACTG GCAGATTTTG 
     3661 GACTTGCTCG GCTCTATAAC TCTGAAGAGA GTCGCCCTTA CACAAACAAA GTCATCACTC 
     3721 TTTGGTATCG ACCTCCAGAG CTTCTTCTTG GAGAGGAAAG ATACACACCA GCCATTGATG 
     3781 TTTGGAGCTG TGGGTGCATC CTTGGAGAAC TGTTCACAAA GAAACCTATT TTTCAAGCCA 
     3841 ATTTAGAACT GGCTCAGCTA GAACTTATCA GTCGTCTCTG TGGTAGTCCT TGTCCAGCAG 
     3901 TGTGGCCTGA TGTTATCAAG CTGCCCTACT TCAACACCAT GAAACCGAAG AAGCAATACA 
     3961 GGAGACGCCT AAGAGAAGAA TTCTCTTTCA TTCCTTCAGC GGCACTTGAT CTATTGGACC 
     4021 ACATGCTGAC ACTGGATCCT AGCAAGAGGT GCACAGCTGA ACAGACCCTA CAGAGTGACT 
     4081 TTCTTAAAGA TGTGGAACTC AGCAAAATGG CACCTCCAGA CCTACCTCAC TGGCAGGATT 
     4141 GCCATGAATT GTGGAGTAAG AAACGTCGAC GGCAGCGACA GAGTGGTATT GTGATAGAAG 
     4201 ATCCACCTCC GTCCAAAGCT TCTAGAAAAG AAACTACCTC AGGGACAACA GCTGAGCCTG 
     4261 TGAAAAACAA TAGCCCAGCA CCACCTCAGC CTGCTCCTGT CAAGGCAGAG CCTGGTCCAG 
     4321 GGGATGCAGT AGGCCTTGGT GACATCACAC AGCAGTTGAA TCAAAGTGAA TTGGCAGTGT 
     4381 TATTAAACCT GCTTCAGAGC CAAACTGACC TGAGCATCCC TCAGATGGCA CAGCTGCTTA 
     4441 ATATCCACTC CAATCCAGAG ATGCAACAGC AGCTTGAAGC CTTGAATCAG TCTATTAGTG 
     4501 CACTGACTGA AGCCAGTTCC CAGCAGCAGG ACTCAGAATC CATAGCCCCT GAAGAATCAT 
     4561 TGAAGGAGGT ACCTTCTGTA CCTGTGGTCC TGCCTCCTGC TGAACAGACA ACTCCTGAAG 
     4621 CTTCAAACAC ACCAGCTGAC ATGCAGAATG TGTTGGCAGT TCTCTTGAGT CAGCTGATGA 
     4681 AAACCCAAGA GCCAGCAGGT AACCTGGAGG AAAACACCAA TGACAAGAAT AGTGGGCCAC 
     4741 AGGGGCCCCG AAGAACTCCT ACAATGCCAC AGGAGGAGGC AGCAGAGAAG AGGCCCCCTG 
     4801 AGCCCCCTGG ACCTCCACCG CCGCCACCTC CACCCCCTCT GGTTGAAGGC GATCTTTCCA 
     4861 GCGCCCCCCA GGAGTTGAAT CCCGCCGTGA CAGCCGCCTT GCTGCAACTT TTATCCCAGC 
     4921 CTGAAGCAGA GCCTCCTGGC CACCTGCCAC ATGAGCACCA GGCCTTGAGA CCAATGGAAT 
     4981 ACTCCACCCG ATCCCATCCA AACAGGACTT ACGGAAATAC TGATGGGCCT GAGACAGGGT 
     5041 TCAGTTCCGC TGACACTGAT GAACGCAGTT CTGGTCCAGC CTTGACAGAA TCTTTGGTTC 
     5101 AGACCCCGGT GAAGAACAGG ACCTTCTCAG GCTCTGTGAG CCACCTTGGG GAGTCCAACA 
     5161 GCTACCAGGG CACAGGGTCA GTGCAGTTCC CAGGGGACCA GGACCTCCGT TTTACCAGGG 
     5221 TTCCCTTAGC ATTACACTCA GTGGTTGGGC AACCATTCCT CAAGTCTGAG GGAAATAGCA 
     5281 ACTCTGTGGT ACATGCAGAG ACCAAATTGC AAAACTATGG GGAGCTGGGA CCGGGAACCA 
     5341 CTGGGGCCAA CAGCTCAGGA ACAACGCTTC AGTGGGGGGG CCCAGCTCAG TCTTATGGAA 
     5401 AACCCTACAG GGGGGCTGCA AGAGTCCTAC CACGAGGGGG AAGAGGGAGA GGAGTTCCTT 
     5461 ATACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5521 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5581 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5641 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5701 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5761 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5821 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5881 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5941 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     6001 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     6061 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     6121 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6181 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6241 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6301 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6361 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6421 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6481 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6541 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6601 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6661 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6721 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6781 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6841 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6901 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6961 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     7021 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     7081 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     7141 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7201 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7261 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7321 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7381 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7441 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7501 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7561 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7621 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7681 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7741 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7801 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7861 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7921 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7981 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     8041 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     8101 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     8161 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8221 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8281 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8341 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8401 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8461 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8521 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8581 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8641 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8701 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8761 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8821 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8881 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8941 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     9001 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     9061 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     9121 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9181 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9241 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9301 TTTAACGCGA ATTTT
//