LOCUS       MR215489                6984 bp    DNA     circular  SYN 
DEFINITION  Nolc1 (Myc-DDK-tagged) - Mouse nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 4
ACCESSION   NM_001039353
VERSION     NM_001039353.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3141..3232 
                     /label="Myc-DDK"  
     misc_feature    3262..3847 
                     /label="PolyA signal"  
     misc_feature    3319..3338 
                     /label="Restriction Enzyme"  
     misc_feature    4123..4795 
                     /label="ColE1"  
     misc_feature    5225..6019 
                     /label="Kan/Neo"  
     misc_feature    6054..6356 
                     /label="SV40 ori"  
     misc_feature    6442..6919 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3123..3129 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3123 
                     /label="Nolc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGATACC GGCTTGCGCC GCGTGGTTCC CAGCGACCTT TATCCCCTTG 
     1081 TGCTCAGATT TCTGCGGGAT AGCCAACTCT CGGAGGTGGC CAGTAAATTT GCAAAAGCGA 
     1141 CCGGCGCTAC ACAGCAGGAC GCCAATGCCT CGTCCCTCTT GGACATCTAT AGCTTCTGGC 
     1201 TCAAGTCCAC CAAAGCCCCA AAGGTGAAGT TACAGTCAAA TGGACCAGTG ACCAAGAAGG 
     1261 CTAAGAAAGA GACTTCATCC AGTGACAGCA GTGAGGACAG CAGTGAGGAC GAGGACAAAA 
     1321 AAGCCCAGGG ACTTCCCACA CAGAAGGCTG CCGCACAGGT CAAGCGAGCC AGTGTGCCTC 
     1381 AGCATGCTGG AAAGGCAGCA GCCAAAGCTT CAGAGAGCAG CAGTAGTGAA GAATCCAGTG 
     1441 AGGAAGAGGA AGAGGACAAA AAGAAAAAGC CTGTCCAGCA GAAGGCAGCT AAGCCCCAAG 
     1501 CCAAGGCAGT CAGACCTCCT GCGAAGAAGG CAGAGAGCTC TGAGTCGGAC TCAGACTCGG 
     1561 ATTCGGACTC CAGCTCAGAG GAAGAAACAC CACAGACCCA GAAGCCAAAG GCAGCTGTGG 
     1621 CAGCAAAAGC TCAGACTAAA GCCGAAGCCA AACCAGGTAC ACCAGCGAAA GCACAGCCTA 
     1681 AGGTAGCCAA TGGCAAAGCA GCCGCCAGCA GCAGCAGCAG CAGCAGCAGC GATGACTCAG 
     1741 AGGAAGAGAA GAAGGCAGCT GCACCTCCCA AGAAGACTGT ACCAAAAAAG CAAGTCGTGG 
     1801 CCAAGGCCCC AGTGAAAGTA GCTGCCGCCC CCACCCAGAA GAGCTCCAGC AGTGAGGATT 
     1861 CTTCCAGTGA AGAGGAGGAG GGACAGAGAC AACCCATGAA GAAAAAAGCA GGTCCCTACA 
     1921 GTTCAGTTCC ACCACCCTCT GTTCCTTTAC CAAAGAAGTC CCCGGGAACC CAGGCTCCAA 
     1981 AGAAAGCTGC TGCGCAGACA CAGCCTGCAG ACAGCAGTGA CGACAGCAGT GACGATTCTG 
     2041 ATTCAAGTTC TGAGGAAGAG AAAAAACCTC CAGCTAAGAC GGTCGTCTCC AAGACACCCG 
     2101 CCAAAGCAGC TCCAGTGAAG AAGAAAGCAG AAAGCTCTTC AGACAGCTCG GATTCTGACA 
     2161 GTTCTGAGGA TGAAGCTCCT GCCAAGCCAG TCAGTACAAC CAAGAGTCCC AAGCCAGCTG 
     2221 TCACTCCGAA GCCATCTGCA GCAAAGGCAG TGACAACTCC TAAGCAACCT GCAGGCAGTA 
     2281 ACCAGAAACC TCAGAGCAGG AAGGCTGACA GCAGCTCCAG CGAGGAGGAA AGCAGCTCCA 
     2341 GCGAGGAGGA GGAGGCCTCC AAGAAAAGTG CCACAACCCC CAAGGCCAAG GTGACTGCTA 
     2401 AAGCAGCACC CGCCAAACAG GCCCCTCAGG CTGCTGGGGA CAGCAGCTCT GACTCAGATA 
     2461 GTTCCAGCAG TGAAGAGGAG GAGAAGACTC CTAAGCCCCC AGCTAAGAAG AAGGCAGCAG 
     2521 GTGGAGCCGT TTCTACACCA GCCCCTGGGA AGAAAGCAGA GGCCAAGAGC AGCAGCAGCA 
     2581 GCAGCAGCAG CAGCTCCGAA GATTCCAGTG AAGAGGAGAA AAAAAAGAAG CCCAAAGCTA 
     2641 CTACCCCTAA AATACAGGCA AGCAAGGCCA ATGGCACTCC AGCTTCTCTG AATGGAAAAG 
     2701 CAGCCAAGGA AAGTGAGGAG GAAGAGGAGG AGGAAGAAAC AGAAGAGAAG AAAAAGGCAG 
     2761 CTGGGACCAA GCCAGGTTCA GGCAAAAAAC GGAAGCAGAA TGAGACCGCA GATGAAGCAA 
     2821 CAACTCCTCA AGCTAAGAAA GTTAAGCTCG AGACCCCCAA TACGTTTCCA AAAAGGAAGA 
     2881 AGGGAGAAAG AAGGGCGTCT TCCCCTTTCC GAAGGGTCAG GGAGGAGGAG ATTGAGGTGG 
     2941 ACTCTCGAGT GGCGGACAAT TCCTTTGATG CCAAGCGAGG TGCAGCTGGA GACTGGGGGG 
     3001 AGCGAGCCAA TCAGGTTCTG AAGTTCACCA AAGGCAAGTC CTTCCGGCAT GAAAAAACGA 
     3061 AGAAGAAGCG AGGCAGCTAC CGGGGAGGCT CCATCTCTGT CCAGGTCAAT TCCGTCAAAT 
     3121 TCGACAGCGA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3181 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3241 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3301 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3361 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3421 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3481 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3541 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3601 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3661 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3721 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3781 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3841 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3901 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3961 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4021 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4081 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4141 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4201 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4261 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4321 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4381 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4441 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4501 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4561 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4621 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4681 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4741 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4801 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4861 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4921 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4981 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5041 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5101 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5161 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5221 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5281 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5341 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5401 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5461 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5521 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5581 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5641 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5701 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5761 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5821 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5881 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5941 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6001 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6061 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6121 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6181 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6241 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6301 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6361 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6421 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6481 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6541 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6601 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6661 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6721 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6781 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6841 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6901 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6961 TAACAAAAAT TTAACGCGAA TTTT
//