LOCUS       MR215465                8754 bp    DNA     circular  SYN 
DEFINITION  Map3k6 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase 6 (Map3k6)
ACCESSION   NM_016693
VERSION     NM_016693.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4911..5002 
                     /label="Myc-DDK"  
     misc_feature    5032..5617 
                     /label="PolyA signal"  
     misc_feature    5089..5108 
                     /label="Restriction Enzyme"  
     misc_feature    5893..6565 
                     /label="ColE1"  
     misc_feature    6995..7789 
                     /label="Kan/Neo"  
     misc_feature    7824..8126 
                     /label="SV40 ori"  
     misc_feature    8212..8689 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4893..4899 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4893 
                     /label="Map3k6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGGCCA TGTCCTGGGG CCGGGGTTCT AGAACGGGCG GGCAGCTGCT 
     1081 GGCAGGACCC GCTGGCGGAG GCGCTGAGCC GGGGCCGCTC GTCTCCTGCG GTCACGGGTC 
     1141 GGGGCTGCGC GCGGAGCCGG CCTCTCAGTG TGGTCTACGT GCTGACCCGG GAGCCGGGGC 
     1201 TCGGGGTGGA GCCCGGGTCG GGAACCGAGG CAGAGCCGCT GCCGTTGCGA TGCCTGCGCG 
     1261 AGGCCTGCGC TCAGCTCCAG GGCACACGGC CACCCCCGCA GCTACGCAGC CTACCCTTTG 
     1321 CGACGCTGGC GCTAGGCGAC ACCGCTGCGC TGGACTCCTT CTACAACGCG GATGTAGTGG 
     1381 TGCTGGAGGT GAGCAGCTCC CTGGCACAGC CTTCCTTGTT CTACCACCTC GGCGTGCGTG 
     1441 AGAGCTTCAG CATGACCAAC AACGTGCTCC TCTGTTCCCA GGCAGAGCTC CCTGATCTGC 
     1501 AGGCCCTTCG TGAGGATGTT TTCCAGAAGA ACTCGGATTG TGTTGGCAGC TACACACTGA 
     1561 TTCCTTATGT GGTGACAGCC ACTGGCCGGG TCTTATGTGG CGATGCAGGC CTCCTGAGGG 
     1621 GCATAGCCGA TGGGCTAGTA CAGGCTGGGG CGGGCACTGA AGCCCTCCTC ACTCCCCTGG 
     1681 TGGGCCGGCT TGTCCGTCTG TTGGAGGCTA CACCCACAGA CTCTTGCGGC TATTTCCGGG 
     1741 AGACCATTCG TCAGGATATC CGGCAGGCTC GGGAGCGATT CAGTGGGCAG CAGCTGAGGC 
     1801 AGGAGCTGGC TCGCCTGCAG CGGAGGCTGG ACAGCGTGGA GCTGCTGAGC CCTGACATTG 
     1861 TCATGAATCT GCTGCTATCG TACCGTGATG TCCAGGACTA CTCGGCCATC ATTGAGCTGG 
     1921 TGGAGACGCT GCAGGCTTTG CCCACCTGTG ATGTGGCTGA ACAGCACAAT GTCTGCTTTC 
     1981 ACTACACATT TGCCCTCAAC AGGAGGAACA GGCCTGGGGA CCGGGAGAAA GCCCTGGCTG 
     2041 TCCTGCTGCC ATTGGTGAAG TATGAAGGTC CCGTGGCTCC GGATCTGTAC TGCATGTGTG 
     2101 GTCGTATCTA CAAGGACATG TTCTTCACTT CTGGCTTCCA AAATGCCGGC CACCTGGAGC 
     2161 AGGCCTATCA CTGGTACCGC AAGGCGTTTG ATGTGGAGCC CAGCCTGCAC TCAGGCATCA 
     2221 ATGCAGCTGT TCTCCTCATT GCAGCTGGGC AGCATTTCGA AGACTCTGAG GAGCTCCGGC 
     2281 TGATTGGCAT GAAGCTGGCC TGCCTGCTAG CCCGCAAAGG CTGTGTGGAG AAGATGCAGT 
     2341 ACTACTGGGA TGTGGGATTC TACCTGGGAG CCCAGATTCT TGCCAACGAC CCCATCCAGG 
     2401 TGGTGCTGGC TGCAGAGCAA CTGTACAAGC TTAATGCCCC CATTTGGTAC TTGGTCTCCG 
     2461 TGATGGAGAC CTTCCTACTG TACCAGCATT TCAGACCCAC ACCAGAGCCC TCAGGAGGAC 
     2521 CCCCGCTGCG AGCTCACTTC TGGCTCCACT TCCTGCTACA ATCTTGCCAG CCTTTCAAGA 
     2581 TGGCTCCTCC TCAGGAGGAC CAGTGCCTGG TACTGGTGCT GGAGATAAAC AAGGTCCTGC 
     2641 TGCCTGCCAG GCTTGAGATT CAGGGAACAG ACCCCGTGAG CACAGTGACC CTAAGCTTGC 
     2701 TGGACCCAGA GACTCAGGAG GATCCTTCCA GCTGGACCTT CCCAGTCACC TCCATCTGTG 
     2761 GGATCAGCAC CTCCAAGCTG GACCAGCGCT GCTGCTTCCT GTACGCACTT CCTCCGGCCC 
     2821 AGGACGTCCA GCTGTGCTTT CCCAGTGTGG AGCGCTGCCA GCGGTTCTGT GGCCTAATTC 
     2881 AGGTCTTGGT GATGAATCCG GATTCCTCCG CGCCCACTGA GGAGGCAGAA GGCGCAAGGG 
     2941 AGGTGCTGGA GTTTGATTAT GAATACTCGG AGACTGGTGA GCGGCTGGTG TTGGGCAGAG 
     3001 GCACGTATGG GGTGGTGTAT GCTGGCCGAG ACAGGCACAC GAGGGTACGA ATCGCCATCA 
     3061 AGGAGATCCC AGAGAGAGAT AGCAGATTCT CTCAGCCTCT GCATGAAGAG ATCGCTCTTC 
     3121 ACAAACGACT TCGTCATAAG AATATTGTGC GTTATCTGGG CTCAGCCAGC CAGGGCGGCT 
     3181 ACCTCAAGAT CTTCATGGAG GAAGTGCCTG GAGGCAGCCT GTCCTCCTTG CTTAGGTCAG 
     3241 TGTGGGGACC CCTAAAGGAC AACGAGAGCA CTATTAGCTT CTATACACGT CAGATCCTAC 
     3301 AAGGACTCAG CTACCTCCAT GAGAACCGTA TCGTTCACAG AGACATCAAG GGAGACAATG 
     3361 TTCTGATCAA CACCTTCAGT GGGCTGCTCA AGATTTCTGA CTTTGGCACC TCTAAGCGGC 
     3421 TGGCAGGCAT CACACCGTGC ACGGAGACTT TCACAGGGAC TCTGCAGTAT ATGGCCCCGG 
     3481 AAATCATCGA CCAGGGCCCG CGAGGATATG GGAAGGCAGC TGACATCTGG TCTCTGGGCT 
     3541 GCACTGTAAT CGAGATGGCA ACAGGTCGAC CGCCCTTCCA TGAACTAGGG AGCCCTCAGG 
     3601 CTGCGATGTT TCAGGTGGGC ATGTACAAGG TACATCCACC CGTGCCCGGT TCCCTGTCAG 
     3661 CTGAGGCCCA AGCCTTCCTC CTCCGAACTT TTGAGCCAGA TCCCCGCCTC CGAGCCAGTG 
     3721 CCCAAGAGCT GCTGGGAGAC CCCTTCCTTC AGCCAGGAAA GAGGAGCCGC AGCCCGGGCT 
     3781 CTCCTCGGCA TACTCCCCGG CCCTCAGGCG CCCCTTCCGG TCCTTCCAGT CCTTCAGCTG 
     3841 ACTCAGCCAC ACAGTCTCAG ACATTCCCAA GGCCCCAGGC ACCCTCTCAG CACCCACCCA 
     3901 GCCCCCCAAA GCGCTGCCTT AGTTATGGGG ACACCAGTCA GCTCCGTGTG CCCGAGGAGC 
     3961 CCGCTGCCGA GGAACCCGCG TCCCCAGAAG AGAGTTCAGG CCTGAGTCTG CTGCACCAGG 
     4021 AGAGCAAGCG CCGGGCCATG CTGGCTGCGG TGCTGGAACA GGAGGTGCCC ACACTAGCAG 
     4081 AGAATCTCCT GGAACAGGAA CAGGACTCTC GACTCAGCAA GATTCATGTG GAACTTCTGC 
     4141 TTCGGTGCCT TGGGGCACAA ATCCACACTC CTAACCGCCG GCAGCTGGCC CAGGAGCTGC 
     4201 GGACCCTGCA AGCTCAGCTG CGGGCCCAGA GCCTGGGCCC TGCGCTTTTG AAGGGGCCGC 
     4261 TCTTCGCCTT TCCAGACGCG GTGAAGCAGA TCCTCCGCAG ACGCCAGATC CGCCCACACT 
     4321 GGATGTTCGT GTTGGACTCG CTGCTCAGCC GTGCAGTCCG GGCGGCGATG GCGGTGCTGG 
     4381 ACGCGGAGTC GGAGAAGAAA GCGGTCTTAC CGAGGTCAGA AGAGTCGAGT AAAGAGGAGT 
     4441 CCCAGCAGAA ACCTCAGGAG AGCCAGGCGC TGCAGAGCCA GCTCCCACCA GAGCAGGGAC 
     4501 CCCCATCGTT GATGGTGGAG TTGGGCCTTT TGCGAGCCGA GACTGACAGG CTTCGGGACC 
     4561 TTCTGGCTGA GAAGGAACGT GAGTGCCAGG CGCTGGTGCA ACAGGCCCTG CATCGGGTGC 
     4621 ATGCAGAGAC CAGGAAGTAT GCCCCAGCTT CAGAGACCCC AGCCACTCTC CCAAAGGACC 
     4681 AGAACCTGGT GCGGTGGCTA CAGGAACTGA GTGTAGATCC AGCCACTATC CAAACGCTCC 
     4741 TGAGTCATAG CTTCACCCTT CAAACCCTGC TCACCTGTGC CACTCAAGAT GATCTAGTGT 
     4801 ACACCAGAAT CAGGGGAGGG ATGGTATGCC GGATTTGGAG AGCCATCTTG GCACAGCGAG 
     4861 CAGGAGCCAC ATCGGTCACC CCAGTACCCC GGGACGCTGA GACGCGTACG CGGCCGCTCG 
     4921 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4981 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5041 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5101 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5161 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5221 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5281 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5341 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5401 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5461 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5521 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5581 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5641 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5701 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5761 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5821 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5881 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5941 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6001 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6061 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6121 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6181 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6241 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6301 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6361 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6421 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6481 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6541 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6601 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6661 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6721 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6781 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6841 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6901 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6961 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7021 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7081 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7141 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7201 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7261 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7321 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7381 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7441 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7501 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7561 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7621 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7681 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7741 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7801 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7861 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7921 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7981 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8041 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8101 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8161 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8221 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8281 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8341 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8401 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8461 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8521 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8581 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8641 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8701 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//