LOCUS       MR215458                8541 bp    DNA     circular  SYN 
DEFINITION  Atp2b3 (Myc-DDK-tagged) - Mouse ATPase, Ca++ transporting, plasma membrane 3 (Atp2b3)
ACCESSION   NM_177236
VERSION     NM_177236.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4698..4789 
                     /label="Myc-DDK"  
     misc_feature    4819..5404 
                     /label="PolyA signal"  
     misc_feature    4876..4895 
                     /label="Restriction Enzyme"  
     misc_feature    5680..6352 
                     /label="ColE1"  
     misc_feature    6782..7576 
                     /label="Kan/Neo"  
     misc_feature    7611..7913 
                     /label="SV40 ori"  
     misc_feature    7999..8476 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4680..4686 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4680 
                     /label="Atp2b3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACATG GCAAACAGTT CCATTGAGTT CCACCCCAAG CCCCAGCAGC 
     1081 AGCGGGAAGT GCCCCATGTG GGTGGCTTTG GATGCACACT GGCAGAACTA CGAAGCCTCA 
     1141 TGGAGCTCCG AGGTGCTGAG GCACTGCAGA AGATTCAAGA AGCCTATGGG GATGTCAGCG 
     1201 GGCTGTGCAG GAGGCTGAAG ACCTCACCTA CTGAGGGCCT GGCAGACAAC ACCAATGACT 
     1261 TGGAGAAACG CAGGCAGATC TATGGGCAGA ACTTCATCCC CCCAAAGCAG CCTAAGACCT 
     1321 TCCTGCAACT GGTGTGGGAA GCCCTGCAGG ACGTGACTCT CATCATCCTG GAGGTGGCTG 
     1381 CTATCGTCTC CCTGGGCCTC TCCTTCTATG CACCTCCTGG AGAGGAAAGT GAAGCCTGTG 
     1441 GGAATGTGTC TGGTGGGGCA GAGGATGAAG GAGAGGCTGA AGCTGGCTGG ATTGAGGGGG 
     1501 CTGCCATCCT ACTCTCTGTC ATCTGTGTGG TGCTGGTCAC AGCCTTCAAT GACTGGAGCA 
     1561 AGGAAAAGCA GTTCCGAGGT CTTCAAAGCC GTATTGAGCA GGAGCAGAAG TTTACTGTCA 
     1621 TACGAAATGG GCAGCTCCTC CAGGTCCCTG TGGCGGCCCT GGTGGTAGGG GACATTGCCC 
     1681 AGGTCAAATA TGGAGATCTT CTGCCTGCCG ATGGTGTGCT CATCCAAGGC AATGACCTCA 
     1741 AGATCGATGA GAGCTCCCTG ACCGGCGAGT CGGACCATGT TCGAAAATCA GCAGACAAAG 
     1801 ATCCTATGCT GCTCTCAGGA ACTCATGTCA TGGAAGGTTC TGGGAGAATG GTGGTAACAG 
     1861 CTGTTGGTGT GAACTCCCAG ACAGGCATCA TCTTTACATT GCTTGGGGCA GGTGGAGAGG 
     1921 AGGAGGAGAA GAAAGACAAG AAAGGCAAGC AGCAGGATGG GGCGATGGAC AGTAGCCAAA 
     1981 CCAGAGCTAA GAAGCAGGAT GGGGCTGTTG CCATGGAAAT GCAACCCCTG AAGAGCGCCG 
     2041 AGGGTGGGGA AATGGAGGAG CGGGAAAAGA AGAAAGCCAA CGTACCCAAG AAGGAGAAGT 
     2101 CAGTCCTGCA AGGAAAGCTC ACAAAACTGG CTGTGCAGAT TGGGAAAGCA GGATTGGTGA 
     2161 TGTCTGCTAT CACTGTCATC ATTCTGGTCC TCTACTTTGT GATTGAGACC TTTGTCGTGG 
     2221 ATGGCCGGGT ATGGCTGGCA GAGTGCACAC CAGTGTATGT GCAGTACTTC GTGAAGTTCT 
     2281 TCATTATTGG AGTCACTGTT TTGGTTGTGG CTGTCCCTGA GGGCCTGCCT CTTGCTGTTA 
     2341 CTATCTCCTT GGCTTATTCT GTCAAGAAAA TGATGAAGGA TAATAACCTG GTACGCCACC 
     2401 TGGATGCCTG TGAGACCATG GGCAATGCCA CAGCCATCTG TTCTGACAAG ACGGGCACAC 
     2461 TCACCACCAA CCGCATGACA GTGGTCCAGT CCTACCTAGG AGACACCCAC TACAAAGAGA 
     2521 TTCCAGCTCC CAGCGCCCTG ACCCCCAAGA TTCTTGACCT TCTGGTTCAC GCCATCTCCA 
     2581 TCAACAGTGC CTACACCACC AAAATTCTAC CTCCAGAGAA AGAAGGCGCT CTCCCACGCC 
     2641 AAGTGGGCAA CAAAACAGAG TGTGCTCTGC TGGGCTTCGT CTTGGACCTG AAACGTGACT 
     2701 TCCAGCCAGT ACGGGAGCAG ATACCAGAAG ATCAGCTTTA CAAAGTGTAC ACCTTCAACT 
     2761 CAGTTCGCAA GTCTATGAGC ACAGTTATCC GCATGCCTGA TGGTGGCTTC CGCCTCTTTA 
     2821 GCAAGGGAGC CTCAGAGATT CTGCTCAAAA AGTGTACAAA CATCTTAAAC AGCAATGGTG 
     2881 AACTCCGAGG ATTTCGTCCT CGGGACCGGG ATGATATGGT GAAGAAGATC ATTGAGCCTA 
     2941 TGGCTTGTGA TGGCCTCCGC ACCATCTGCA TTGCCTACAG GGACTTCTCT GCTATCCAGG 
     3001 AGCCTGATTG GGACAATGAG AATGAGGTGG TTGGTGACCT TACCTGCATA GCTGTTGTGG 
     3061 GCATCGAGGA CCCTGTGCGA CCTGAGGTCC CTGAAGCCAT TCGAAAATGC CAGCGTGCTG 
     3121 GCATCACAGT CCGTATGGTG ACTGGAGACA ACATCAACAC TGCCCGGGCT ATTGCAGCTA 
     3181 AGTGTGGCAT CATCCAGCCA GGAGAGGACT TCCTGTGCCT GGAGGGGAAG GAATTCAACA 
     3241 GAAGAATTCG AAATGAGAAA GGCGAGATTG AACAGGAGCG GCTGGACAAA GTGTGGCCCA 
     3301 AGCTGCGGGT GCTTGCCCGG TCATCTCCCA CTGATAAACA TACTCTGGTT AAAGGCATTA 
     3361 TTGACAGCAC AACTGGTGAG CAGCGGCAGG TGGTGGCTGT GACTGGAGAT GGCACCAATG 
     3421 ATGGGCCAGC CCTCAAAAAG GCAGATGTGG GCTTCGCCAT GGGCATTGCA GGCACTGATG 
     3481 TGGCCAAGGA GGCCTCTGAC ATCATTCTGA CTGATGACAA CTTCACCAGC ATTGTCAAGG 
     3541 CGGTCATGTG GGGCCGCAAT GTCTATGACA GCATCTCCAA GTTCCTGCAG TTTCAGTTGA 
     3601 CAGTCAACGT GGTAGCTGTG ATCGTGGCCT TCACGGGTGC CTGCATTACT CAGGACTCTC 
     3661 CTCTCAAAGC TGTGCAGATG TTGTGGGTGA ACTTAATCAT GGACACATTT GCCTCACTTG 
     3721 CCCTGGCAAC GGAACCCCCA ACTGAGTCAC TGCTGCTGCG GAAGCCATAC GGCCGGGACA 
     3781 AGCCTCTCAT CTCGAGAACC ATGATGAAGA ACATCCTTGG ACATGCTGTC TACCAGCTCA 
     3841 CCATCATCTT TACCCTGCTA TTTGTTGGGG AGCTTTTCTT TGACATTGAC AGTGGAAGAA 
     3901 ATGCACCTCT GCACTCACCA CCGTCAGAGC ACTATACCAT CATCTTCAAC ACGTTCGTCA 
     3961 TGATGCAGCT TTTCAATGAG ATCAATGCTC GCAAGATCCA TGGTGAGAGG AATGTCTTTG 
     4021 ATGGCATCTT CAGCAACCCC ATCTTCTGCA CCATTGTCTT GGGCACCTTT GGAATTCAGA 
     4081 TTGTCATTGT CCAATTTGGA GGGAAGCCCT TCAGCTGTTC CCCACTGTCC ACAGAACAGT 
     4141 GGCTTTGGTG CCTGTTTGTT GGTGTTGGGG AGCTGGTCTG GGGACAGGTC ATTGCCACCA 
     4201 TCCCCACCAG CCAGCTCAAG TGCCTGAAGG AAGCAGGGCA TGGGCCTGGG AAGGATGAGA 
     4261 TGACTGATGA AGAGCTGGCC GAAGGGGAAG AAGAAATTGA CCATGCTGAA CGAGAGCTCC 
     4321 GCAGGGGCCA GATCCTCTGG TTTCGGGGCC TCAACCGGAT CCAGACACAG ATCCGGGTGG 
     4381 TGAAAGCATT CCGTAGCTCG CTTTATGAAG GCCTGGAGAA ACCAGAATCC AAGAGTTGCA 
     4441 TCCATAACTT CATGGCAACA CCCGAGTTTT TGATCAATGA CTACACCCAC AATATCCCGC 
     4501 TCATCGATGA CACAGATGTG GATGAGAACG AAGAGCGCCT GAGGGCCCCA CCTCCCCCAC 
     4561 CCCCTAACCA GAACAACAAC GCCATAGACA GCGGCATCTA CCTGACCACG CATGCCACCA 
     4621 AGTCAGCTAC CTCTTCAGCA TTCTCTTCCA GGCCCGGGAG CCCACTCCAC AGCATGGAGA 
     4681 CATCCCTCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4741 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4801 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4861 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4921 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4981 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5041 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5101 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5161 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5221 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5281 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5341 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5401 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5461 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5521 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5581 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5641 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5701 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5761 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5821 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5881 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5941 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6001 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6061 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6121 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6181 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6241 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6301 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6361 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6421 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6481 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6541 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6601 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6661 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6721 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6781 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6841 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6901 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6961 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7021 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7081 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7141 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7201 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7261 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7321 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7381 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7441 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7501 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7561 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7621 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7681 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7741 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7921 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7981 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8041 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8101 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8161 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8221 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8281 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8341 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8401 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8461 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8521 CAAAAATTTA ACGCGAATTT T
//