LOCUS       MR215441                7434 bp    DNA     circular  SYN 
DEFINITION  Macc1 (Myc-DDK-tagged) - Mouse metastasis associated in colon cancer 1 (Macc1)
ACCESSION   NM_001163136
VERSION     NM_001163136.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3591..3682 
                     /label="Myc-DDK"  
     misc_feature    3712..4297 
                     /label="PolyA signal"  
     misc_feature    3769..3788 
                     /label="Restriction Enzyme"  
     misc_feature    4573..5245 
                     /label="ColE1"  
     misc_feature    5675..6469 
                     /label="Kan/Neo"  
     misc_feature    6504..6806 
                     /label="SV40 ori"  
     misc_feature    6892..7369 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3573..3579 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3573 
                     /label="Macc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTAATCAGT GAAAGAGCCT ATTGTTGGCC ACGAGAAATT GGTCGAAGTA 
     1081 GATCTGAAGG AAATTTGATT GACATGGAAG CCTCGAAACG CTCAAAAAAT CAAAATATTA 
     1141 CAGCACGGCA AGGGCTAGGC TTGCCTCACA ACTGGCCAGA TGCTCCTACC CTCCATCGTG 
     1201 ATAGTGATGC CAAAGCTACA AATCCATTCT GGAAGGAACT GTCTGCCTCA AACCCATTTC 
     1261 TGGAGGACAT CACGCAGCTA AGAAACAACA AAAAGAGAGA TAATATGTCT ATCTTGAAGG 
     1321 AAGATCCTTT TCTTTTTTTA AGACAAATAG AAACAGAAAA TTCTTTTGAC TCCTCTGGTG 
     1381 ATGAACTTGA CATGGATCAG GGGTTTAAGT CATCTTCCTC TCGGAAGTCA GGAAGGTCTA 
     1441 AGAGTGTTTC TGAACTCCTG GATGTTTTAG GTGATAGGAC ACACACTCAT CAAAGTATAC 
     1501 ATAGCTCTGA TCAGATCCTA ATACAAGACT TAGAATGGCT ACAAAATGAC CGAGAAGCAT 
     1561 ATAAAATGGC TTGGCTTAGT CAACGTCAGT TGGCTCGCTC TTGCCTAGAT CTGAATACAA 
     1621 TTAATCAGAG TCCTGGGTGG GCTCAAACAC AAATTGCAGA GACCATTGCA GTTTGTAAAT 
     1681 TAAATCACCA AGGAGGGTCA GTCCAATTAC CCGACTCAGA CATCACTATT CATATACCCC 
     1741 AAGGACATAT AGCTGTGGGA GAATTCCAGG AGGTATCTCT GAGAGCATTT CTGGATCCTC 
     1801 CACCAAAGCT GAACCATGAT CTCTCTTGTA CTGTAAGCCC TCTCTTGGAA ATCAGGTTAA 
     1861 GCAATCTGAA TACCATGGAA GCTATTTTGT TGGAGATGAA AACTGGAGCA GAAGTAAAAA 
     1921 CAGATCCTCT GAGCCAAGTC ATGACTAAAA TGGTGTGTTT GTACAGCCTG GACAGAGAAG 
     1981 GCCCTTTCCA AGCATTAAAC ACCTGCTATA TCTACAAAGA TACCATCCAA GCTAAGCTTC 
     2041 TTGACCTGAG CCATGTAATG TATATAGTCA TAGCTGTACA AGCTACGATC ATTCATCCAC 
     2101 CAGCTGCTAC TATTTGGGAT TATATCCACA AAACCACCTC TATTGGAATT TATGGACCTA 
     2161 AATATCTCCA TCCAAGCTTT ACTGCTGTTT TCACTGTTTG TGGACACAAC TATGTGCCAG 
     2221 GAAAGTTTAC AGTCTCTGAC ATTAGGAAGG GTGGAAAAAA CACATCTCCA GTCGTATTTC 
     2281 ACCTCTGGGG GAAGCATTCA TTTTCACTTG ACAAGCCAAA AGATTTAATT ATTTCAGCTT 
     2341 TTTCGTGTGA TCCTGATTTT GAAGTAAAGG AAGAGGGAGA AAGAAAAGTA ATTGCACAAA 
     2401 GGCAATTGGA AGCAGGTGTG GTAGTTCATC AACAAGTTTT CTTTTCTTCA ATTGATCACA 
     2461 GAGAGATGCA CTTGTTTGTT TTCCGGGTTC AGGTAAAGAC CCCCAATGAA AAACCAGTGA 
     2521 CACAGTTCTT TGTCACTTCA CCTGATCCAT CCCCAAATCT AAAAAGGTTC TCATATCTGC 
     2581 CAGGATTTTT CCAGAAGAGA GAAGACAAGA GAGTGCCTTT ATTGTCGACA GGACTTGTTA 
     2641 AGTATCCCAC TTTCCAAGAT AAAAAATTGA ACTTTACCAA TTATGGGGTT GCACTGAAGA 
     2701 CAGTACTGAG GCAAAATAAG ATTGACTATT TGCTTGAATA TTTAAAAGGG GACACAATTG 
     2761 CCCTTCTTGG AGAAGGTACA GTAAGAGCCA TAGGACAGTC CAAAGTGAAA GAATGGTACG 
     2821 TAGGAGTCCT CCGAGGTAAG ATCGGACTCG TGCATTGTAA AAATGTCAAA GTGATTTCTA 
     2881 AAGAACAAGT GATGTCAATG TCTGATGGCA TTTTGACAAC CAAGAATCTT CTGGAACAAA 
     2941 TTGCCTTGCC ATTTAAAAGT CTGACTTATA TATACTCTGT TGTATTGACC TTGGTGTCAG 
     3001 AAAAAGTTCA CGACTGGAAA GTTTTAGCTA ATGTCCTGGG TTACTCACAT CTGGCACAGG 
     3061 AAGATTTTAA TAGAATTCAA GCAGACAAGG AACCAGAAAG GGTTTCTTAC GTTGTCAAGA 
     3121 AGTTAAAGGA AGATTGCCAT GAAGATAGAA ATACCAGGAA GTTCCTTTAT GAACTGATTG 
     3181 TGGCTCTGCT AAAAATGGAT TGCCAAGAGT TAGTGGCACA TCTTATCCAA GAGGCTGTTA 
     3241 TTCTGACTTC AGCTGTGAAA CTTGGAAGAA GCTGGAGGGA ACTTGCTGAG AAATCTGCAG 
     3301 GACTCACTAA GCATCAGATG CAGGCATATG AAATTCCTCA CCGAGGAAAA TCTGGAGATG 
     3361 TTTCAGCAGA GATGATGTGG AAACCTGCCT ATGATTTTCT CTATGCATGG AGTTTTCACT 
     3421 ATGGAAATAG CTACAGAGAT GTGTTACAAG ATCTTCAATC AGCTTTGGAC AGAATGAAAA 
     3481 ACCCTGTCAC TAAACGGTGG AGAGATTTAA CTGGAACTTT AATACTTATA AATTCTTTAG 
     3541 AGATTCTGAG AGTGACTGCT TTCTCTACTT CTCAGGAAAT AACGCGTACG CGGCCGCTCG 
     3601 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3661 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3721 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3781 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3841 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3901 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3961 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4021 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4081 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4141 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4201 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4261 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4321 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4381 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4441 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4501 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4561 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4621 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4681 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4741 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4801 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4861 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4921 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4981 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5041 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5101 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5161 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5221 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5281 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5341 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5401 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5461 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5521 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5581 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5641 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5701 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5761 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5821 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5881 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5941 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6001 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6061 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6121 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6181 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6241 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6301 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6361 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6421 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6481 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6541 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6601 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6661 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6721 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6781 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6841 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6901 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6961 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7021 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7081 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7141 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7201 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7261 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7321 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7381 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//