LOCUS       MR215417                8457 bp    DNA     circular  SYN 
DEFINITION  Cgn (Myc-DDK-tagged) - Mouse cingulin (Cgn)
ACCESSION   NM_001037711
VERSION     NM_001037711.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4614..4705 
                     /label="Myc-DDK"  
     misc_feature    4735..5320 
                     /label="PolyA signal"  
     misc_feature    4792..4811 
                     /label="Restriction Enzyme"  
     misc_feature    5596..6268 
                     /label="ColE1"  
     misc_feature    6698..7492 
                     /label="Kan/Neo"  
     misc_feature    7527..7829 
                     /label="SV40 ori"  
     misc_feature    7915..8392 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4596..4602 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4596 
                     /label="Cgn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCTGAT CCCCGGGGCC CCGTAGACCA TGGAGTTCAA ATTCGATTTA 
     1081 TTACAGAGCC AGAGGGTGCT ACTGAAATGG GCACCCTTCG TCGTAGTGGG CGGCGCCCTG 
     1141 CAAGGGATGC AAGAGCCAGT ACCTATGGGG TCGCTGTGCG TGTGCAGGGC ATCGCCGGGC 
     1201 AACCGTTTGT GGTTCTTAAC AGTGGAGAGA AAGGGACTGA CTCCTTTGGG GTCCAGATCA 
     1261 AAGGGGGCAA CAACCGGGGG TCCCCAGGTG CACTGAGCTC CGATTCAGAG CTCCCCGAAA 
     1321 ACCCCTACTC TCAAGTCAAG GGACGCCCTG CCACTTCTCG GAGCAGCACA TCTGATGAGG 
     1381 AGCCCAAGGA CCACTTGAAT GGAAAACTGA TCCGTTCCCA GTCGCAAGCC TCTCTGACCG 
     1441 GGCTTGCCTT TATGAGTCCT AGTAATAGGA GCACCAGCTT GCTGGAACTA GCCCCTAAAC 
     1501 CGACTTCCTC GATTAACACC ATTGACACTG CCCCCTTGTC TTCAGTGGAC TCGCTCATCA 
     1561 ACAAGTTTGA TAGTCAGAAG GGGGGGCAGG TCCGGGGTCG CACTGGGCGC CGCACAAGGA 
     1621 CACTTCCCCA CGAACAGCGC AAGCGGAGCC AGAGTCTGGA CAGCAGGCTC CCACGGGATA 
     1681 CACGTGAGGA ACGGGAGCAC CAGTCCGCTA ACCACTGGAC CCGCGGCACA AAGTATGACA 
     1741 ACCATGTGGA CAGTTCAAAG AACCCATCGC AGAAGCAGAG CCCGTTCAGC AGCTTCAGCC 
     1801 GTTCGCGTCA GACTCAAGAC TGGGTGCTTC AGAGTTTTGA GGAGACACGG GATCCAGCCA 
     1861 TGGTGCAGTT CAAATCAACT CCAGACCTTC TCAGAGACCA GAGAGAGACA GCGCCACCAG 
     1921 GCAGTGCGGA CCATGTGAAG GCCACCATCT ATGGCATCCT AAGGGAGGGA AGCTCGGAGA 
     1981 GTGAGGCATC TGTGAGGAGG AAAGTTAGTT TGGTGCTGGA GCAAATGCAG CCTCTGGGGA 
     2041 TGGTTTCTCC TGCTTCTACC AAGGCCCTGG CAGGACAGGC TGAGCTTACC CGAAAAATGG 
     2101 AGGAACTTCA GAAGAAGCTG GATGAGGAGG TGAAGAAGCG GCAGAAGCTA GAACCATCCA 
     2161 GGGTTGGGCT GGAGAGGCAG CTGGAGGAGA AGGCTGAAGA GTGCCACCGA CTGCAGGAGT 
     2221 TGCTGGAGAG GAGAAAGGGG GAGGTCCAGC AGAGCAGCAA GGAACTTCAG AACATGAAGC 
     2281 TCCTGCTAGG CCAGGAAGAA GGACTACGGC ATGGGCTGGA GGCCCAGGTG AAGGAGCTAC 
     2341 AGCTTAAGCT CAAGCATAGC CAGAGTCCCG ATTCTGGAAA GGAAAGTCTC CTAAAGGACT 
     2401 TGTTAGACAC CCGGGAACTT CTAGAAGAAC TCTTAGAGGG CAAACAACGA GTAGAGGAGC 
     2461 AGCTCAGGCT GAGGGAACGG GAGCTGACAG CCCTGAAGGG GGCCCTGAAA GAGGAGGTGG 
     2521 CCTCCCACGA CCAGGAGGTG GAACATGTCC GGCTGCAGTA CCAGCGAGAC ACGGAGCAGC 
     2581 TTCGCCGGAG CATGCAAGAT GCGACCCAGG ACCATGCTGC ACTGGAGGCT GAGAGGCAGA 
     2641 AGATGTCTTC CCTCGTGCGG GAGCTGCAAA GAGAGCTAGA GGAGACCTCT GAGGAGACAG 
     2701 GGCATTGGCA GAGCATGTTC CAGAAGAACA AGGAAGAGCT GAGAGCCACC AAGCAGGAAC 
     2761 TGCTGCAACT GCGGATGGAG AAGGAGGAGA TGGAAGAGGA ACTTGGGGAG AAGATGGAGG 
     2821 TCTTACAGAG GGACTTAGAG CAGGCCCGAG CCAGTACAAG AGATACTCAC CAGGTTGAGG 
     2881 AACTTAAAAA GGAGCTTCGA CGCACCCAGG GGGAGCTTAA AGAGCTTCAG GCAGAGCAGC 
     2941 AGAACCAGGA GGTGACTGGG CGACACCAGA ACCAGGTGTT AGAGAAGCAG CTGGCAGCGC 
     3001 TGAGGGAGGA GGCTGATCGA GGCCGAGAGC TGGAGCAGCA GAACCTCCAG CTACAAAAGA 
     3061 CCCTGCAGCA ACTGCGGCAG GACTGTGAAG AGGCTTCCAA GGCTAAGGTG GCATCAGAGA 
     3121 CAGAGGCCAT GGTGCTGGGG CAGCGTCGGG CAACCGTGGA AACCACACTT CGGGAGACAC 
     3181 AAGAGGAAAA TGACGAATTC CGAAGGCGCA TCCTGGGTCT GGAGCAGCAG TTGAAGGAGG 
     3241 CCCGAGGTCT GGCGGAAGGT GGGGAAGCGG TGGAGGCACG GCTTCGGGAC AAAGTGCATC 
     3301 GGCTGGAGGT AGAGAAACAG CAGCTGGAAG AGGCTTTGAA TGCTGCCCGG GAGGAGGAGG 
     3361 GGAACCTGGC AGCAGCCAAG CGGGCTCTGG AGGTCCGGCT AGATGAGGCC CAGCGGGGGC 
     3421 TGGCACGCCT TGGGCAGGAA CAGCAAGCAC TGAACCGGGC CCTAGAGGAA GAGGGGAAGC 
     3481 AGCGGGAGGC TCTCCGGAGG AGCAAGGCAG AGTTGGAGGA GCAGAAGCGT TTGCTGAACA 
     3541 GGACTGTAGA CCGACTCAAC AAAGAGCTGG AGCAGATTGG GGATGACTCC AAGCTAGCCC 
     3601 TGCAGCAGCT CCAGGCTCAG ATGGAAGATT ACAAGGAAAA GGCCCGGAAG GAAGTAGCTG 
     3661 ATGCCCAGCG TCAGGCCAAG GATTGGGCCA GTGAGGCTGA AAAGAATTCA GGGGGGCTGA 
     3721 GCCGGCTTCA GGATGAGCTG CAAAGGCTGC GGCAAGCCCT GCAGACATCC CAGGCTGAGC 
     3781 GGGACACAGC CAGGCTGGAC AAAGAGCTGC TGGCCCAGCG ACTGCAGGGT CTAGAGCAAG 
     3841 AGGCTGAGAA CAAGAAACGC TTCCAGGACG ACAAGGCTCG ACAGCTGAAG AGCCTAGAGG 
     3901 AGAAAGTCTC GCGGCTGGAA GCGGAGTTAG ATGAAGAAAA GAACACTGTG GAACTGTTAA 
     3961 CAGATCGGGT GAACCGTGGC CGGGATCAGG TGGATCAACT GAGGACAGAG CTCATGCAGG 
     4021 AGAGATCTGC TCGACAGGAC TTGGAGTGTG ACAAAATCTC CTTGGAGAGA CAGAACAAGG 
     4081 ATTTGAAGAC CCGGTTGGCC AGTTCAGAGG GCTTCCAGAA ACCCAGCGCT AGCCTCTCCC 
     4141 AGCTTGAGTC CCAGAATCAG CTGCTGCAAG AGCGGCTGCA AGCTGAAGAG AGAGAGAAGA 
     4201 CAGTTCTGCA GTCCACCAAT CGCAAACTAG AACGGAGGGT TAAGGAGCTC TCCATCCAGA 
     4261 TTGATGATGA ACGGCAGCAT GTCAACGACC AGAAGGACCA GCTAACCCTG AGGGTGAAGG 
     4321 CTTTGAAGCG GCAGGTGGAT GAAGCAGAAG AGGAAATTGA GCGGCTGGAC AGCCTGAGGA 
     4381 AGAAGGCACA GCGGGAACTG GAGGAACAGC ATGAGGTCAA TGAACAGCTC CAAGCCCGGA 
     4441 TCAAATCCTT GGAAAAGGAT GCCTGGCGGA AAGCTTCCCG CTCAGCTGCT GAGTCAGCCC 
     4501 TCAAACAAGA GGGGCTGAGC TCTGATGAGG AGTTTGACAA CGTCTACGAC CCCTCATCCA 
     4561 TTGCATCACT GCTTACTGAG AGCAACCTGC AGACTAGCTC ATGTACGCGT ACGCGGCCGC 
     4621 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4681 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4741 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4801 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4861 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4921 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4981 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5041 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5101 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5161 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5221 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5281 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5341 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5401 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5461 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5521 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5581 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5641 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5701 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5761 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5821 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5881 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5941 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6001 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6061 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6121 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6181 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6241 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6301 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6361 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6421 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6481 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6541 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6601 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6661 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6721 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6781 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6841 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6901 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6961 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7021 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7081 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7141 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7201 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7261 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7321 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7381 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7441 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7501 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7561 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7621 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7681 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7741 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7801 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7861 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7921 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7981 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8041 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8101 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8161 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8221 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8281 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8341 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8401 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//