LOCUS       MR215405                6549 bp    DNA     circular  SYN 
DEFINITION  Mageb4 (Myc-DDK-tagged) - Mouse melanoma antigen, family B, 4 (Mageb4)
ACCESSION   NM_001033492
VERSION     NM_001033492.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2706..2797 
                     /label="Myc-DDK"  
     misc_feature    2827..3412 
                     /label="PolyA signal"  
     misc_feature    2884..2903 
                     /label="Restriction Enzyme"  
     misc_feature    3688..4360 
                     /label="ColE1"  
     misc_feature    4790..5584 
                     /label="Kan/Neo"  
     misc_feature    5619..5921 
                     /label="SV40 ori"  
     misc_feature    6007..6484 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2688..2694 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2688 
                     /label="Mageb4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCAGGGGA CAAAAGAGTA AGGCCCGTGC TCGTGAGAAG CGTCGCCTGG 
     1081 TCCAGGATGA GGCCCAGGAG CTCCAGGATG CTCAAGCCAA GGCAGGAGAG AAAGGAAAGT 
     1141 CCCCCTCCTG CTCTAATCAG GATTCTGGAG ATGCTGTTGC AAGCACCTCT ACTGCTGGCT 
     1201 TCCCTCAAAA GTCTAAATCT CAAGGTGAGG CACCCACCAC CACTGCTAGG AAAGGTGGTG 
     1261 CCTGCAGAAG ATCTCGTAAA AGTACCAGAG GTCCAAGGGA GGAAAGTACC AGTTGCACTA 
     1321 GGGTCCCCCG CTTCAGTGAG AATCCACAGA ATGATCTTCT AACAAGGAAG ACAGGAATGC 
     1381 TGATGCAGTA CCTGCTCTGC AAGTACAAAA TGAAACAGCC AGCATCTAAG GGAGAAATGC 
     1441 TCAAAGTGAT CAACAGAAGG TTCAAGGAGC AGTTGCCTGA GATCCTCAAG AAAGCCTCTG 
     1501 AGCGCATTCA GCTAGTTTTT GGTCTTGAGG TGAAAGAAAT TAAGCCCAAT GGTGGCTACT 
     1561 ACACCCTTGT TAGCAAGTTA GATCCCAGTG TCGGTAATGC TCTGACCACT AGCCTGCCAT 
     1621 TTCCCCAGAA CGGGCTTTTG ATGCCTCTTC TGGGTGTGAT CTTCTTAAAT GGCAACCGTG 
     1681 CCTCTGAGGC AGAGATCTGG GAATTCCTTA ATGTCTTGGG AATTTATGAT GGGAAGGTGC 
     1741 ACATAATCTT TGGGGAGCCC CGGAAGCTCA TCACCAGAGA TCTGGTTAAG GAAAAGTACC 
     1801 TGGTATACCA AAAGGAGGCC AACAGTGATC CCCCATCCTT TGAGTTCCTT TGGGGTCCCC 
     1861 GAGCCTATGC TGAAACTACC AAGATGAAAA TCCTGGAGTT TTTAGCCGAG GTCAATGAGA 
     1921 CCGTTCCTCA GGCCTTCCCA ACCCATTATG AGGAGGCTTT GAGAGATCAG GAAGAGAGAG 
     1981 CCCAAGCCGA AGCTGTAGGC AGTCCTGGCA CTAGTGCCAA AGATAAGGCA GAGGCTAAAG 
     2041 TCACACTGGT TGACTCCTCT TGCAAATATC AGGCTGAGTC TAAAGTCACA CTAGTGGACT 
     2101 CTTCTTGTAA AGATCAGGCT GAGTCTAAAG TCACACTTGT TGACCCCTCT TGCAAAGATA 
     2161 ATGCCAAGTC TAAGGTCACA CTTGGCTCCT CTCGCAAATA CAAGGCCAAG TCTAAAGTCC 
     2221 CACTGGTTGA CTCCTCTTGC AAAGATCAGG CTGAGTCTAA AGTCACACTT GTGGACTCTT 
     2281 GCAAAGATCA GGCTGAGTCT AAAGTCACAC TAGTGGACTC TTCTTGTAAA GATCAGGCTG 
     2341 AGTCTAAAGT CACACTAGTG GACTCTTCTT GTAAAGATCA GGCTGAGTCT AAAGTCACAC 
     2401 TTGTTGACCC CTCTTGCAAA GATAAGGCCA AGTCTAAGGT CACACTTGGC TCCTCTCACA 
     2461 AATACAAGGC CAAGTCTAAA GTCACACTGG TTGACTCCTC TTGCAAAGAT CAGGCTGAGT 
     2521 CTAAAGTCAC ACTAGTGGAC TCTTCTTGTA AAGATCAGGC TGAGTCTAAA GTCACACTTG 
     2581 TTGACCCCTC TTGCAAAGAT AATGCCAAGT CTAAGGTCAC ACTTGGCTCC TCTCGCAAAT 
     2641 ACAAGGCCAA GTCTAAAGTC CCACTGGTTG ACTCCTCTGG AAAAGATAAG GCAGTCACGC 
     2701 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     2761 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     2821 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     2881 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     2941 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3001 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3061 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3121 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3181 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3241 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3301 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3361 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     3421 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     3481 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     3541 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     3601 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     3661 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     3721 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     3781 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     3841 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     3901 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     3961 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4021 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4081 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4141 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4201 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4261 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4321 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4381 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     4441 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     4501 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     4561 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     4621 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     4681 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     4741 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     4801 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     4861 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     4921 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     4981 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5041 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5101 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5161 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5221 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5281 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5341 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     5401 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     5461 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     5521 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     5581 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     5641 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     5701 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     5761 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     5821 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     5881 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     5941 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6001 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6061 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6121 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6181 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6241 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6301 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6361 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     6421 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     6481 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     6541 GCGAATTTT
//