LOCUS       MR215393                7758 bp    DNA     circular  SYN 
DEFINITION  Pcsk6 (Myc-DDK-tagged) - Mouse proprotein convertase subtilisin/kexin type 6 (Pcsk6)
ACCESSION   NM_011048
VERSION     NM_011048.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3915..4006 
                     /label="Myc-DDK"  
     misc_feature    4036..4621 
                     /label="PolyA signal"  
     misc_feature    4093..4112 
                     /label="Restriction Enzyme"  
     misc_feature    4897..5569 
                     /label="ColE1"  
     misc_feature    5999..6793 
                     /label="Kan/Neo"  
     misc_feature    6828..7130 
                     /label="SV40 ori"  
     misc_feature    7216..7693 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3897..3903 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3897 
                     /label="Pcsk6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCCGCGC GCGCCGCCAG CGCCCGGGCC CCGGCCGCCG CCCCGGGCCG 
     1081 CCGATGTCGC GCGGGGCGCA GGGGCCGCCG GCCGGCACGG ACTCCCGCCG CTCGCGCTGC 
     1141 GCCCCTGGCG TTGGCTGCTT CTGCTCGCGC TGCCCGCCGC CTGCTCCGCG CTGCCGCCGC 
     1201 CGCGCCCCGT CTACACCAAC CACTGGGCAG TGCAAGTGCT GGGCGGCCCC GGCGCGGCGG 
     1261 ACCGCGTGGC TGCGGCGCAC GGCTACCTCA ACTTGGGCCA GATTGGAAAC CTGGACGATT 
     1321 ACTATCATTT TTACCACAGC AAGACCTTCA AGAGATCGAC CTTGAGCAGC AGGGGCCCCC 
     1381 ACACCTTCCT CAGAATGGAC CCACAGGTAA AATGGCTGCA ACAACAGGAA GTGAAGCGCA 
     1441 GAGTCAAGAG ACAGGCGCGA AGTGACTCTC TTTATTTCAA TGATCCCATT TGGTCTAACA 
     1501 TGTGGTACAT GCATTGTACT GATAAGAACA GTCGCTGTCG GTCAGAGATG AACGTCCAGG 
     1561 CGGCGTGGAA GCGTGGCTAC ACAGGAAAGA ATGTGGTCGT CACCATCCTC GATGACGGCA 
     1621 TAGAAAGGAA TCACCCAGAC CTGGCCCCCA ACTACGATTC CTATGCAAGC TACGATGTCA 
     1681 ATGGAAATGA TTATGACCCA TCCCCGAGAT ATGACGCCAG CAACGAGAAC AAACATGGCA 
     1741 CTCGCTGTGC GGGTGAAGTC GCTGCCTCGG CCAACAACTC CTACTGCATC GTGGGCATCG 
     1801 CATATAATGC AAAGATAGGA GGCATCCGGA TGCTGGACGG CGATGTGACC GATGTGGTGG 
     1861 AGGCCAAGTC TCTGGGCATC AGACCCAACT ACATTGACAT TTACAGCGCC AGTTGGGGAC 
     1921 CCGATGATGA CGGAAAGACA GTAGATGGAC CAGGCCGGCT GGCTAAACAA GCTTTCGAGT 
     1981 ATGGCATTAA AAAGGGTCGC CAAGGTCTGG GCTCCATTTT TGTTTGGGCC TCTGGGAATG 
     2041 GTGGGAGAGA AGGGGACCAC TGCTCCTGTG ATGGCTACAC CAACAGCATC TACACCATCT 
     2101 CCGTAAGCAG CACCACTGAG AACGGCCACA AACCCTGGTA CCTGGAAGAA TGCGCTTCCA 
     2161 CGTTGGCTAC CACCTACAGC AGCGGGGCCT TCTATGAACG GAAGATCGTC ACCACAGATC 
     2221 TGCGTCAGCG CTGCACCGAC GGCCACACTG GGACATCAGT CTCAGCCCCC ATGGTGGCTG 
     2281 GTATCATTGC CTTGGCTCTA GAAGCAAACA ACCAGTTGAC CTGGAGGGAT GTGCAGCACC 
     2341 TGCTGGTGAA GACATCACGG CCAGCTCATC TGAAGGCGAG CGACTGGAAA GTCAACGGAG 
     2401 CTGGGCATAA AGTTAGCCAT CTCTATGGAT TTGGCTTGGT GGATGCAGAA GCTCTGGTCC 
     2461 TGGAGGCGAG GAAGTGGACG GCAGTGCCCT CCCAGCACGT ATGCGTGGCC ACCGCAGACA 
     2521 AAAGGCCCAG GAGCATCCCC ATAGTGCAGG TGCTACGGAC CACAGCCCTG ACCAACGCCT 
     2581 GTGCGGACCA CTCAGACCAG CGTGTGGTGT ACCTGGAGCA TGTGGTTGTC CGAATCTCTA 
     2641 TCTCACACCC ACGCCGGGGC GATCTCCAGA TCCACCTGAT TTCTCCCTCT GGAACCAAGT 
     2701 CTCAACTTTT GGCAAAGAGA TTGCTGGATT TTTCCAATGA GGGGTTCACA AACTGGGAAT 
     2761 TCATGACCGT CCACTGCTGG GGAGAAAAGG CTGAAGGGGA GTGGACTCTG GAAGTGCAGG 
     2821 ATATACCATC GCAGGTCCGA AACCCAGAGA AACAAGGAAA GCTGAAAGAA TGGAGCCTCA 
     2881 TTTTATATGG CACCGCAGAG CACCCATATC GCACCTTCAG CTCCCATCAG TCTCGCTCAC 
     2941 GGATGCTGGA GCTCTCCGTC CCGGAGCAGG AGCCTCCCAA GGCTGCTGGA CAACCACCCC 
     3001 AGGCAGAGAC TCCAGAAGAC GAGGAAGAGT ACACAGCTCC TTCCATCCAA GGCTCTCCTA 
     3061 ATACTTTACA GACCAGTGTG TGCCATCCCG AGTGTGGTGA CAAAGGCTGT GATGGTCCCA 
     3121 ATGCAGACCA GTGCTTGAAC TGTGTCCACT TCAGCCTGGG GAACTCCAAG ACAAACAGGA 
     3181 AGTGTGTGAG CGAGTGCCCC TTGGGCTACT TTGGGGATGC AGCTGCAAGA CGCTGCCGTC 
     3241 GCTGCCATAA GGGATGTGAG ACGTGCACGG GCAGGAGCCC AGCACAGTGC CTGTCTTGTC 
     3301 GCCGTGGGTT CTATCACCAC CAGGAGACGA ACACGTGTGT GACCCTCTGT CCTGCCGGAC 
     3361 TTTATGCTGA TGAAAGTCAG AGACTCTGCC TCAGGTGCCA CCCGAGCTGT CAGAAGTGCG 
     3421 TGGATGAACC TGAGAAGTGC ACTGTGTGTA AGGAGGGATT CAGCCTGGCA CGGGGCAGCT 
     3481 GCATTCCGGA CTGTGAACCG GGTACCTACT TCGATTCAGA GCTCGTCAAA TGTGGGGAAT 
     3541 GCCATCACAC CTGCCGGACC TGTGTGGGGC CCAGCAGAGA AGAGTGTATT CACTGTGCAA 
     3601 AAAGCTTCCA CTTCCAAGAC TGGAAATGTG TGCCGGCCTG CGGTGAGGGC TTCTACCCGG 
     3661 AGGAGATGCC TGGCTTACCC CACAAAGTGT GTCGAAGATG TGAGGAGAAC TGCCTGAGCT 
     3721 GCGAGGGCTC CAGTAGGAAC TGCAGCAGAT GTAAAGCCGG CTTCACACAG CTGGGAACCT 
     3781 CCTGCATCAC CAACCACACG TGCAGCAATG CCGATGAGAC CTTCTGCGAG ATGGTGAAGT 
     3841 CCAATCGGCT CTGCGAACGG AAGCTCTTCA TCCAGTTTTG CTGCCGCACC TGCCTCCTGG 
     3901 CTGGGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3961 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4021 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4081 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4141 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4201 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4261 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4321 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4381 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4441 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4501 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4561 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4621 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4681 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4741 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4801 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4861 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4921 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4981 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5041 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5101 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5161 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5221 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5281 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5341 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5401 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5461 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5521 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5581 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5641 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5701 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5761 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5821 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5881 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5941 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6001 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6061 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6121 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6181 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6241 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6301 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6361 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6421 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6481 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6541 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6601 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6661 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6721 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6781 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6841 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6901 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6961 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7021 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7081 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7141 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7201 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7261 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7321 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7381 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7441 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7501 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7561 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7621 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7681 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7741 AAATTTAACG CGAATTTT
//