LOCUS       MR215340                8337 bp    DNA     circular  SYN 
DEFINITION  Man2a2 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha 2 (Man2a2)
ACCESSION   NM_172903
VERSION     NM_172903.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4494..4585 
                     /label="Myc-DDK"  
     misc_feature    4615..5200 
                     /label="PolyA signal"  
     misc_feature    4672..4691 
                     /label="Restriction Enzyme"  
     misc_feature    5476..6148 
                     /label="ColE1"  
     misc_feature    6578..7372 
                     /label="Kan/Neo"  
     misc_feature    7407..7709 
                     /label="SV40 ori"  
     misc_feature    7795..8272 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4476..4482 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4476 
                     /label="Man2a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCTGAAA AAGCAGGTGA CGGTGTGTGG GGCTGCTATC TTCTGTGTGG 
     1081 CCGTCTTTTC TCTGTACCTT ATGCTGGACC GAGTGCAGCA TGATCCTGCC AGACACCAGA 
     1141 ATGGTGGGAA CTTCCCCAGG AGCCAAATCT CTGTGCTACA GAACCGGATA GAACAGCTGG 
     1201 AACAACTGCT GGAGGAAAAC CATGACATCA TAAGCCGTAT CAAGGACTCT GTACTGGAAC 
     1261 TGACAGCCAA TGCGGAGGGC CCGCCAGCCC TGCTGCCCTA CCATACCGCC AACGGTTCCT 
     1321 GGGCTGTGCT CCCGGAGCCC CGGCCCAGCT TCTTCTCCGT CTCCCCCCAG GACTGCCAGT 
     1381 TTGCTTTGGG GGGACGGGGT CAGAAGCCAG AACTACAGAT GTTAACCGTG TCTGAGGATT 
     1441 TGCCGTTTGA CAATGTGGAG GGTGGCGTGT GGAGGCAAGG CTTTGACATC TCCTACAGCC 
     1501 CAAATGACTG GGACACTGAA GACCTGCAAG TGTTTGTGGT GCCTCACTCC CACAACGATC 
     1561 CAGGCTGGAT CAAGACTTTT GACAAGTACT ACACGGAACA GACCCAGCAC ATCCTCAACA 
     1621 GCATGGTGTC CAAGCTGCAG GAAGACCCCC GGCGGCGCTT TCTCTGGGCA GAAGTCTCCT 
     1681 TCTTCGCCAA GTGGTGGGAC AACATCAGTG CCCAAAAGAG GGCAGCAGTT CGAAGGCTGG 
     1741 TGGGAAATGG GCAGCTGGAG ATTGCGACAG GCGGATGGGT GATGCCCGAC GAGGCCAACT 
     1801 CCCATTACTT TGCCTTGGTT GACCAGCTCA TCGAGGGGCA CCAGTGGCTG GAGAGAAACC 
     1861 TGGGTGCAAC CCCGCGCTCG GGCTGGGCAG TGGACCCCTT TGGACACAGC TCCACCATGC 
     1921 CTTACCTGCT GCGCCGTGCC AACCTGACCA GCATGCTGAT TCAGAGGGTG CATTATGCCA 
     1981 TCAAGAAGCA CTTTGCTGCC ACTCATAGCC TGGAGTTCAT GTGGAGGCAG ATGTGGGATT 
     2041 CAGACTCCAG CACAGACATC TTTTGCCACA TGATGCCCTT CTACAGCTAC GACGTTCCAC 
     2101 ACACCTGTGG CCCCGATCCC AAGATCTGCT GCCAGTTTGA TTTCAAACGT CTGCCGGGTG 
     2161 GGAGAATCAA TTGCCCTTGG AAGGTGCCAC CCCGGGCTAT TACAGAAGCC AATGTGGCAG 
     2221 ACAGGGCAGC CCTGCTCCTG GACCAGTACC GGAAGAAGTC CCGGCTGTTC CGAAGCAACG 
     2281 TCCTCCTCGT GCCACTTGGT GATGACTTCC GGTATGACAA GCCCCAGGAA TGGGATGCCC 
     2341 AGTTCTTCAA CTACCAGCGG CTCTTTGACT TCCTCAACAG CAAGCCTGAG TTCCATGTGC 
     2401 AGGCCCAGTT TGGGACCCTC TCTGAGTATT TCGATGCCCT GTATAAGAGG ACAGGAGTAG 
     2461 AGCCTGGTGC CCGGCCTCCA GGGTTTCCTG TGCTGAGTGG GGACTTCTTC TCCTACGCTG 
     2521 ACCGGGAGGA CCACTACTGG ACAGGCTATT ACACCTCCCG GCCTTTCTAC AAGAGCTTGG 
     2581 ACCGCGTCCT AGAAGCCCAC CTGCGTGGGG CAGAGATTCT ATACAGCCTG GCTTTGGCCC 
     2641 ATGCCCGCCG TTCTGGACTG GCCGGCCAGT ACCCGCTGTC TGATTTTGCT CTTCTGACGG 
     2701 AAGCTCGGCG TACACTGGGC CTCTTCCAGC ACCACGATGC CATCACGGGA ACCGCCAAGG 
     2761 AGGCAGTAGT GGTAGACTAC GGGGTCAGGT TGCTGCGTTC CCTGGTCAGC CTCAAGCAGG 
     2821 TTATCATCAA TGCTGCCCAC TACCTGGTGC TGGGGGACCA GGAGACCTAC AGCTTTGACC 
     2881 CTGGGACACC CTTCCTCCAA ATGGATGACA GCCGTGTAAG CCATGATGCC CTGCCCGAGC 
     2941 GCACGGTGAT CCGGCTGGAC TCCTCACCCA GGTTTGTGGT GGTATTTAAC CCGCTGGAAC 
     3001 AGGAGCGGCT CAGTGTGGTG TCCCTGCTGG TCAACTCGCC CCGGGTGCGC GTCCTTTCAG 
     3061 AGGAGGGCCA GCCCCTGTCT GTGCAGATCA GTGTGCACTG GAGCTCGGCT ACGGACATGG 
     3121 TCCCTGATGT CTACCAGGTG TCAGTACCCG TCCGCCTGCC AGGCCTGGGC CTGGGTGTGC 
     3181 TGCAGCTGCA GCCAGATCTC GATGGGCCCT ACACGCTGCA GTCTTCCGTG CGGGTCTACC 
     3241 TGAACGGCGT GAAACTGTCC GTCAGCAGGC AGTCAGCATT CCCTGTCCGT GTTGTGGACT 
     3301 CCGGCGCCAG CGACTTCGCC ATCAGCAATC GCTACATGCA GGTCTGGTTC TCCGGCCTTA 
     3361 CTGGGCTTCT CAAGAGCATC CGACGGGTGG ATGAGGAGCA GGAGCAGCAG ATGGAACTGG 
     3421 AGTTCCTCGT CTACGGCACC CGTACCTCCA AGGACAAGAG TGGCGCCTAC CTCTTCCTGC 
     3481 CTGATAGCGA GGCTAAGCCC TACGTCCCTA AGAAACCTCC TGTGCTGCGT GTCACCGAAG 
     3541 GCCCTTTCTT CTCAGAGGTG GCTGTGTACT ATGAGCACTT TCACCAAGTG ATTCGACTTT 
     3601 ATAACCTGCC AGGGGTAGAG GGGCTGTCTC TGGACATGTC ATTCCAGGTA GATATCAGGG 
     3661 ACTATGTGAA CAAGGAACTG GCCCTGCGCA TCCACACAGA CATTGACAGC CAGGGCACTT 
     3721 TCTTCACAGA CCTCAATGGC TTTCAGATAC AGCCCCGGCA GTATCTGAAG AAGCTGCCCC 
     3781 TGCAGGCTAA TTTCTACCCT ATGCCAGTCA TGGCCTATAT CCAAGATTCC CAGAGGCGCC 
     3841 TCACGCTGCA CACTGCCCAG GCTCTGGGTG TCTCCAGCCT CGGTAATGGC CAGCTGGAGG 
     3901 TGATCTTGGA CCGAAGGCTA ATGCAGGATG ACAACCGGGG ACTAGGCCAA GGGCTCAAAG 
     3961 ACAACAAGAT CACCTGCAAC CGTTTCCGCC TCCTGTTAGA ACGTCGGACC ACAATGAGCC 
     4021 CAGAGGTGCA TCAGGAGCAG GAGCGTTCTA CAAGCTACCC GTCCCTCCTC AGCCACCTGA 
     4081 CATCCATGTA CCTCAGCACA CCTCCTCTTG TCTTACCCGT GGCCAAGAGG CAGGGCACTA 
     4141 GCCCTGCTCT GCGCTCTTTC CACCCTCTGG CTTCTCCATT GCCCTGTGAC TTCCATTTGC 
     4201 TCAATCTGCG CATGCTCCCA GCCGAGGACA CCTTGCCCGC AACTGACTCT GCGCTCATCT 
     4261 TACACCGCAA GGGGTTTGAC TGTGGCCTTG AAGCCAAGAA CCTGGGCTTC AACTGTACCA 
     4321 CAAGCCAAGG CAAGCTAGCC CTGGGGAGCC TCTTCCATGG CCTGGATGTG ACCTTCCTGC 
     4381 AGCCAACCTC CTTGACTTTG CTATACCCTC TGGCCTCACC TTCCAACAGC ACTGACATCT 
     4441 CTCTGGAGCC CATGGAGATC AGCACCTTCC GCCTGCGCTT GGGCACGCGT ACGCGGCCGC 
     4501 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4561 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4621 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4681 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4741 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4801 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4861 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4921 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4981 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5041 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5101 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5161 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5221 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5281 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5341 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5401 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5461 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5521 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5581 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5641 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5701 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5761 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5821 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5881 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5941 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6001 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6061 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6121 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6181 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6241 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6301 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6361 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6421 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6481 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6541 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6601 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6661 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6721 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6781 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6841 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6901 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6961 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7021 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7081 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7141 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7201 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7261 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7321 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7381 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7441 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7501 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7561 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7621 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7681 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7741 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7801 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7861 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7921 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7981 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8041 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8101 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8161 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8221 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8281 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//