LOCUS       MR215283                8379 bp    DNA     circular  SYN 
DEFINITION  Tnks2 (Myc-DDK-tagged) - Mouse tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 (Tnks2)
ACCESSION   NM_001163635
VERSION     NM_001163635.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4536..4627 
                     /label="Myc-DDK"  
     misc_feature    4657..5242 
                     /label="PolyA signal"  
     misc_feature    4714..4733 
                     /label="Restriction Enzyme"  
     misc_feature    5518..6190 
                     /label="ColE1"  
     misc_feature    6620..7414 
                     /label="Kan/Neo"  
     misc_feature    7449..7751 
                     /label="SV40 ori"  
     misc_feature    7837..8314 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4518..4524 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4518 
                     /label="Tnks2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGGCCGC CGCTGCGCCG GCGGGGGCGC GGCCTGCGCG AGCGCCGGGG 
     1081 CCGAGGCCGT GGAGCCGTCC GCCCGCGAGC TGTTCGAGGC TTGCCGCAAC GGGGACGTGG 
     1141 AGCGAGTCAA GAGGCTGGTG ACGCCCGAGA AGGTGAACAG CCGCGACACG GCGGGCAGGA 
     1201 AGTCCACCCC GCTCCACTTC GCCGCAGGTT TTGGACGGAA GGATGTAGTT GAATATCTTC 
     1261 TTCAGAACGG TGCAAATGTG CAAGCGCGTG ATGATGGGGG TCTTATTCCT CTTCATAACG 
     1321 CATGCTCTTT TGGTCATGCT GAAGTAGTCA ATCTTCTTTT ACAACACGGT GCAGACCCAA 
     1381 ATGCTCGGGA TAATTGGAAT TATACTCCTC TCCATGAAGC TGCAATTAAA GGAAAAATTG 
     1441 ATGTTTGCAT TGTGCTTTTA CAGCATGGAG CTGAGCCAAC CATCCGAAAT ACAGATGGAA 
     1501 GGACAGCATT GGATTTAGCA GACCCATCTG CCAAAGCTGT TCTGACTGGT GACTATAAGA 
     1561 AAGATGAACT TCTGGAAAGT GCCAGGAGTG GCAATGAGGA AAAAATGATG GCCCTGCTTA 
     1621 CACCATTGAA TGTCAACTGC CATGCAAGTG ATGGCAGAAA GTCAACTCCA TTGCATTTGG 
     1681 CAGCAGGATA CAACAGAGTA AAAATTGTAC AACTGTTACT GCACCATGGG GCTGATGTCC 
     1741 ATGCTAAAGA TAAAGGGGAT CTGGTACCAC TACACAATGC CTGCTCTTAC GGTCATTATG 
     1801 AAGTAACTGA ACTTCTGGTC AAGCACGGTG CCTGTGTGAA TGCAATGGAC CTGTGGCAGT 
     1861 TCACTCCTCT TCATGAGGCA GCTTCTAAGA ACAGGATTGA AGTATGTTCT CTCCTCCTAA 
     1921 GTTACGGGGC AGATCCAACC CTGCTGAACT GTCACAATAA AAGTGCTATA GACCTGGCCC 
     1981 CCACGGCACA GCTGAAGGAA AGATTATCGT ATGAATTTAA AGGCCATTCA TTGCTGCAAG 
     2041 CTGCACGAGA AGCTGATGTT ACAAGAATCA AAAAACATCT TTCTCTGGAA ATGGTGAATT 
     2101 TCAAACATCC TCAGACACAT GAAACAGCAC TGCATTGTGC TGCTGCATCT CCATATCCCA 
     2161 AAAGAAAGCA GATATGTGAA CTGCTGCTCA GAAAGGGAGC AAACACCAAC GAAAAGACTA 
     2221 AAGAATTCTT GACTCCTCTG CACGTGGCTT CTGAGAACGC TCACAATGAT GTTGTTGAAG 
     2281 TAGTGGTGAA ACATGAAGCA AAGGTTAATG CTTTGGATAG TCTTGGACAG ACGTCATTAC 
     2341 ACAGAGCTGC ACACTGTGGT CACCTGCAGA CCTGCCGCCT GCTCCTGAGC TATGGGTGTG 
     2401 ATCCAAACAT CATATCCCTT CAGGGTTTCA CCGCCTTGCA AATGGGAAAT GAAAATGTGC 
     2461 AGCAGCTGCT CCAAGAGGGC GCCTCACTAG GTCACTCAGA GGCGGACAGA CAGCTGCTGG 
     2521 AAGCTGCAAA GGCTGGTGAC GTTGAAACTG TAAAAAAACT CTGTACTGTT CAGAGTGTCA 
     2581 ACTGCAGAGA CATTGAAGGA CGCCAGTCAA CCCCACTCCA CTTTGCAGCT GGGTACAACA 
     2641 GAGTGTCTGT GGTAGAATAT CTGCTGCAAC ATGGAGCTGA TGTTCATGCT AAAGACAAAG 
     2701 GAGGTCTTGT ACCTTTACAC AATGCATGTT CTTATGGACA CTATGAAGTT GCAGAACTTC 
     2761 TTGTCAAGCA TGGAGCAGTA GTCAATGTCG CTGACTTGTG GAAGTTCACA CCTTTACATG 
     2821 AAGCTGCAGC AAAAGGAAAA TATGAAATAT GCAAACTTCT ACTCCAGCAT GGTGCAGACC 
     2881 CTACAAAGAA AAACAGAGAT GGTAATACTC CTCTGGATCT TGTTAAAGAT GGAGATACAG 
     2941 ATATTCAAGA TCTACTTAGA GGTGATGCAG CTTTGCTGGA TGCTGCCAAG AAGGGTTGTT 
     3001 TAGCCAGAGT GAAGAAATTG TCTTCTCCTG ATAATGTAAA TTGCCGTGAT ACCCAAGGTC 
     3061 GGCATTCTAC ACCTTTACAT TTGGCAGCTG GTTATAATAA CTTGGAAGTT GCTGAGTATT 
     3121 TATTGCAACA TGGAGCTGAT GTGAATGCCC AGGACAAAGG AGGACTCATT CCTTTACATA 
     3181 ACGCAGCATC GTATGGACAT GTAGATGTAG CAGCTTTGCT GATAAAGTAC AATGCGTGTG 
     3241 TCAATGCCAC GGACAAATGG GCTTTCACAC CGTTACATGA AGCAGCCCAA AAGGGACGGA 
     3301 CACAGCTTTG TGCTTTATTG TTGGCCCATG GAGCTGATCC TACTCTTAAA AATCAAGAAG 
     3361 GACAAACACC TTTAGATTTA GTTTCAGCAG ATGATGTCAG TGCACTCTTG ACAGCAGCCA 
     3421 TGCCCCCCTC TGCTCTGCCT ACGTGCTACA AACCTCAAGT GCTCAGTGGC GTGAGGGGCC 
     3481 CCGGAGCCAC TGCAGATGCT CTGTCTTCAG GTCCGTCCAG CCCATCCAGC CTCTCTGCAG 
     3541 CCAGCAGCCT CGACAACTTA TCTGGCAGCT TCTCGGAACT GTCCGCAGTG GTTAGTTCAA 
     3601 GTGCAGCAGA AGGTGCTACT GGTTTGCAAA GAAAAGAGGA TTCAGGAATC GATTTTAGTA 
     3661 TAACTCAGTT CATAAGGAAC CTTGGACTTG AGCACTTGAT GGATATATTT GAGAGAGAAC 
     3721 AGATTACCTT AGATGTCCTA GTTGAAATGG GCCACAAGGA ACTGAAAGAG ATTGGAATCA 
     3781 ATGCCTATGG ACATCGACAC AAGCTGATTA AAGGAGTTGA AAGGCTGATC TCTGGACAAC 
     3841 AAGGTCTTAA TCCATATTTA ACTCTAAACA ACTCTGGTAG TGGAACAATT CTCATAGATC 
     3901 TGTCTCCTGA TGATAAAGAA TTTCAGTCTG TGGAAGAAGA GATGCAGAGT ACTGTGCGGG 
     3961 AGCACAGAGA TGGTGGTCAC GCAGGCGGCG TCTTCAACAG ATACAACATT CTCAAGATTC 
     4021 AGAAGGTTTG TAACAAGAAA TTGTGGGAAA GATATACACA CCGGAGAAAA GAAGTTTCTG 
     4081 AAGAAAACCA CAACCATGCA AACGAAAGGA TGTTATTTCA TGGGTCTCCT TTTGTGAATG 
     4141 CGATTATCCA TAAGGGCTTT GATGAAAGGC ATGCATACAT AGGTGGCATG TTTGGAGCTG 
     4201 GAATTTATTT TGCTGAGAAC TCTTCCAAAA GCAATCAATA TGTGTATGGA ATTGGAGGTG 
     4261 GCACCGGATG TCCAATTCAC AAAGACAGAT CGTGTTACAT TTGTCACAGG CAGCTGCTGT 
     4321 TTTGTCGAGT AACCTTGGGA AAGTCTTTCT TGCAGTTCAG CGCAATGAAA ATGGCACATT 
     4381 CTCCTCCCGG CCATCACTCG GTCACTGGCC GGCCCAGTGT AAATGGCCTA GCATTAGCTG 
     4441 AATATGTTAT TTACAGAGGA GAACAGGCTT ATCCTGAATA TCTAATTACT TACCAGATTG 
     4501 TAAGGCCTGA AGGTATGGTT GATGGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4561 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5341 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5401 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6001 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6061 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6121 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6181 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6241 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6301 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6361 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6421 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6481 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6541 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6601 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6661 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6721 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6781 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6841 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6901 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6961 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7021 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7081 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7141 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7201 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7261 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7321 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7381 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7441 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7501 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7561 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7621 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7681 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7801 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7861 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7921 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7981 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8041 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8101 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8161 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8221 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8281 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8341 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//