LOCUS       MR215134                7617 bp    DNA     circular  SYN 
DEFINITION  Abi3bp (Myc-DDK-tagged) - Mouse ABI gene family, member 3 (NESH) binding protein (Abi3bp), transcript variant 3
ACCESSION   NM_001014399
VERSION     NM_001014399.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3772..3863 
                     /label="Myc-DDK"  
     misc_feature    3893..4478 
                     /label="PolyA signal"  
     misc_feature    3950..3969 
                     /label="Restriction Enzyme"  
     misc_feature    4754..5426 
                     /label="ColE1"  
     misc_feature    5856..6650 
                     /label="Kan/Neo"  
     misc_feature    6685..6987 
                     /label="SV40 ori"  
     misc_feature    7073..7550 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3747..3754 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3747 
                     /label="Abi3bp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCTCCAGC CTGGGGTGTC TACTTCTCTG TGGAAGTATT GCACTTGCTC 
     1081 TGGGAAATGC ACAGAAATTG CCAAAAGGTA AAAAGCCAAG CTTGAAAGTG CACATCAATA 
     1141 CCACCAGCGA CTCCATCCTC TTGAAGTTCC TGCGTCCAAA TGCAAACGTA AAGCTGGAAG 
     1201 GTTTTCTCCT GGGATATGGC AGCAATGTGT CTCCAAACCA GTACTTCCCT CTTCCCACAG 
     1261 AAGGGAAATT CACAGAGGCT GTAGTTGATG CAGAGCCGAA GTATCTGATA GTCGTACGAC 
     1321 CTGCTCCTCC TCCCAGCCAG AAGAAGTCAT GTTCAGGGAA ATCTCGCCCT CGCAAACCCC 
     1381 TCCAGCTGGT GGTGGGTACG TTGACACCGA GTTCTGTCTT CCTGTCCTGG GGATTCCTCA 
     1441 TCAACCCACA CCATGACTGG ACATTGCCAA GCCACTGTCC CAGTGACAGA TTTTATACAA 
     1501 TTCGATATAG AGAAAAAGAT AAAGAAAAGA AATGGATTTT TCAACTCTGC CCAGCTACTG 
     1561 AAACAATTGT GGAAAATCTA AAGCCCAACA CAGTTTATGA ATTTGGTGTG AAAGACAATA 
     1621 TAGAAGGTGG AATTTGGAGT AAGATTTTCA ATCATAAGAC TATTGTTGGA AGTAAAAACA 
     1681 AAGTAAACGG GAAAATCCAA AGCACTTATG ACCAGGTCCA CTCAGTGCCA GTCCCGAGGA 
     1741 AGCTAATCCC ACTAACGATC ATTAAGCAAG TGATTCAGAA TGTTACTCAC AGGGCTTCAA 
     1801 CCAAATCCCC AGACAAGACT CCTTTTGGGG GAACAATATT AGTTCATCTG ATTATTCCGG 
     1861 GTCTTAATGA ATCCACTGTA AAACTTCCCA CATCCATAAT GCTTGAGATT TCCGATGCTC 
     1921 TCAAGGCACA ATTAGCTAAG AATGAAACAT TGGCTTTACC AGCAGAATCT AAAACACCAG 
     1981 AGGTCGAAAA ATTGGCAGGG CAGCCAGTAA CAGTGACTCC GGAATCAGTT TCAAGAAGCA 
     2041 CTAAGCCCAC CCTGTCTAGT GCTTTAGACA CTGCAGAGAC AGCACTGGCA CCCAGGCAGA 
     2101 CAACCAGCAT GCCTCCCAAG CTCAAAACAC CACACTCAAG GATGCCAGCA AAAGAGCCAG 
     2161 TTCCTAAGGA ACCCCTGCAT ACGACTTCAA AGCCAAAAAT GCCACCAAGT CCTGAAGTGG 
     2221 CAGACACTAC ATCTGCACCT TTAGAGACAC GGGGCATTCC TCTCATACCT GTGATTTCAC 
     2281 CAAGACCTAG TCAAGAGGAA CTACAAACCG CCATGGAAGA AACAGACCAG TCTACCCAAG 
     2341 AGCTCTTCAC GACTAAGATT CCACGAACAA CTGAATTGGC AAAGACAACT CAGGCACCAC 
     2401 ACAGATTGCA TACAGCTCCT GTGAGGCCCA GAATACCTGG CAGGCCACAT GGCAGGCCTG 
     2461 CTCTGAACAA AACAACTACA AGGCCTGATA AAACCAAACC CAGAGGGACG TCCCATAAAA 
     2521 ACGGAGTAGG AACAGGAACC AAGCAAGCAC CTAAGCCACC AAGTCCTGGG AGAAATGCGT 
     2581 CAGTGGATTC ACATGCCACA AGAAAACCAG GCTCAGTCTC AGGGACGCGT CGTCCACCCA 
     2641 TACCACATAG GCATTCGTCC ACTAGACCTG TGTCTCCAGA GAGAAGGCCT TTACCCCCGA 
     2701 ATAATGTCAC CGGGAAGCCA GGACGTGCGG GAATTGTTTC CTCCAGCCGG GTAACGTCCC 
     2761 CACCCCTGAA GGCAACACTC CACCCTATCG GAACAGCCAC AGCGAGACCA GGGGCAGAAC 
     2821 AAAAGGAACC AACTGCTCCT GCTTCAGAAG AAGAGTTTGG TACTACAACT GACTTCAGTT 
     2881 CAAGTCCCAC CAAAGAAACT GATCCCCTTG GGAAGCCCAG GTTCATAGGT CCCCATGTGC 
     2941 GATATATCCC AAAGCCTGAA AACAAGCCCT GTTCCATCAC TGACTCAGTC AGACGGTTCC 
     3001 CTACAGAGGA AGCCACGGAG GGGAATGCCA CCAGCCCACC ACAGAACCCA CCCACCAACC 
     3061 TCACTGTGGT CACTGTCGAA GGCTGCCCCT CATTTGTCAT CTTGGACTGG GAAAAACCTC 
     3121 TAAATGACAC TGTCACTGAA TATGAAGTCA TATCCAGAGA AAATGGGTCA TTCAGTGGGA 
     3181 AGAACAAGTC CATTCAAATT ACCAATCAAA CGTTCTCTAC AGTAGAAAAC CTGAAACCAG 
     3241 ATACAAGCTA TGAATTCCAG GTGAAACCCA AAAATCCACT TGGTGAGGGC CCCGCCAGCA 
     3301 ACACGGTAGC GTTCAGTACT GAATCAGCTG ACCCAAGAGT GAGTGAGCCA ATTTCTGCAG 
     3361 GAAGAGATGC CATCTGGACT GAAAGACCTT TTAACTCAGA CTCTTACTCA GAATGCAAAG 
     3421 GCAAACAGTA TGTTAAAAGG ACGTGGTATA AAAAATTTGT CGGAGTGCAG CTATGTAACT 
     3481 CTCTCAGATA CAAGATTTAT TTGAGCGATT CCCTCACAGG CAAGTTTTAC AACATAGGAG 
     3541 ATCAGAGGGG CCACGGAGAA GATCACTGCC AGTTTGTGGA TTCATTTTTA GATGGTCGCA 
     3601 CAGGACAGCA ACTCACCTCT GAACAGTTAC CCACCAAAGA AGGCTATTTC AGAGCTGTTC 
     3661 GTCAGGAACC TGTCCAATTT GGAGAAATAG GTGGTCATAC CCAAATCAAT TATGTCCAGT 
     3721 GGTATGAATG TGGGACCACA ATACCTGGAA AATGGAGCGG ACCGACGCGT ACGCGGCCGC 
     3781 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3841 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     3901 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     3961 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4021 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4081 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4141 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4201 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4261 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4321 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4381 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4441 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4501 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4561 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4621 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4681 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4741 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4801 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4861 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     4921 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     4981 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5041 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5101 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5161 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5221 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5281 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5341 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5401 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5461 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5521 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5581 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5641 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5701 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5761 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5821 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     5881 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     5941 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6001 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6061 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6121 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6181 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6241 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6301 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6361 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6421 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6481 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6541 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6601 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6661 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6721 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6781 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6841 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6901 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     6961 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7021 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7081 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7141 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7201 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7261 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7321 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7381 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7441 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7501 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7561 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//