LOCUS       MR214927                7521 bp    DNA     circular  SYN 
DEFINITION  Gareml (Myc-DDK-tagged) - Mouse family with sequence similarity 59, member B (Fam59b)
ACCESSION   NM_001167879
VERSION     NM_001167879.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3678..3769 
                     /label="Myc-DDK"  
     misc_feature    3799..4384 
                     /label="PolyA signal"  
     misc_feature    3856..3875 
                     /label="Restriction Enzyme"  
     misc_feature    4660..5332 
                     /label="ColE1"  
     misc_feature    5762..6556 
                     /label="Kan/Neo"  
     misc_feature    6591..6893 
                     /label="SV40 ori"  
     misc_feature    6979..7456 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3660..3666 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3660 
                     /label="Garem2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGCTG GCGGCCGGGC TGGCCGGGCT GCGCTGGAGC ATGGGCGCTT 
     1081 TCCCGCTGGA CCTCATCGTC AGCCGCTGCC GCCTGCCCAC GCTCGCCTGC CTCGGACCAG 
     1141 GAGAGTATGC TGAGGGAGTC AGTGAACGAG ACATCCTGCT GATCCACTCC TGCCGCCAGT 
     1201 GGACTACAGT GACTGCCCAC ACCCTGGAGG AGGGCCACTA TGTCATTGGG CCCAAGATTG 
     1261 ACATCCCCCT GCAGTACCCA GGCAAATTCA AGCTCCTGGA GCAGGCTCGG GACGTGCGGG 
     1321 AGCCTGTAAG ATACTTCAGC AGCGTGGAGG AGGTGGCCAG TGTCTTCCCT GACCGAATCT 
     1381 TCGTGATGGA AGCCATCACC TTCAGTGTCA AGGTGGTGTC AGGCGAGTTC AGCGAGGACA 
     1441 GCGAGGTGTA CAACTTCACA CTGCACGCAG GCGACGAGCT CACTCTCATG GGCCAGGCGG 
     1501 AGATTCTGTG CGCCAAGACG ACCAAGGAGC GCTCGCGCTT CACCACGCTC CTGCGCAAGC 
     1561 TGGGCCGTGC AGGGGCCTTG GCCGGAATCG GCGGCCCCGG GAGCATGGGA GCAACAGGGG 
     1621 GTGGCGGCGG GGCCGCCAGG CCCGTCAAAA GCAAGATGCC CTGTCTCATC TGCATGAACC 
     1681 ACCGCACCAA CGAGAGCCTG AGCCTGCCCT TCCAGTGCCA GGGCCGATTC AGCACGCGCA 
     1741 GCCCACTGGA GCTGCAGATG CAGGAGGGCG AGCACACGGT GCGCGCCATC ATCGAGCGCG 
     1801 TGCGGCTGCC GGTGAACGTG CTGGTGCCCA GCCGGCCGCC GCGCAACCCC TACGACCTCC 
     1861 ACCCGGTGCG GGAGGGACAC TGCTACAAGC TGGTCAGCAT CATCTCCAAG ACGGTGGTGC 
     1921 TGGGGCTGGC GCTGCGCCGC GAGGGCCCGG CGCCGCTGCA CTTCCTGCTG CTTACGGACA 
     1981 CGCCGCGCTT CACTCTGCCG CAGGGTCTGC TGGCCGGGGA CCCGCGCGTC GAGCGCCTGG 
     2041 TGCGCGACAG CGCCTCCTAC TGCCGCGAGC GCTTCGATCC GGACGAATAC TCCACTGCTG 
     2101 TGCGCGAGGC GCCCGCGGAG CTCTCCGACG ACTGCGCCAG CCCGCGCCAT GCGCGCCTCT 
     2161 GCCTGCCCGC GCCGCGCGCC CCCGGGGCCG TCCGTGCGCC TGGACCGCCG GGTCGCCTGG 
     2221 GTCCCGCTTT GCCCGCGCCC GGCGACAGCG ATCAGGATTA CGTGAGCCCC GATTGGGCGG 
     2281 GTGTGTCCGA GCCAGCGGGC GGCTGCGCCG AGATCCCTTA CGAAGAGCTG TGGGTGCACC 
     2341 AAGCCCCCGA GAGCCGCGCC GACGCCCGTG CACGGCCGCT AGCGGGGCCG GACCTCATCT 
     2401 CCTTCGGGAT CGTGGGGCCC CCACGGCATG AGCCAGAGGC ACCACCACCT CCTGTCCCTC 
     2461 CCAAATCCGA GGCGGTGAAG GAAGAGTGCC GCCTGCTTCA CGCCCCTCCA GTGCCTCCTA 
     2521 GGGGTGGCGG CAGCTGCAGC AAGCTCACAG GCAGCCCTCC GGTGCTGCCC CACTTTCCCA 
     2581 AGCTGCAGCC TGTCCACTCG CCCAGCTCCA GTCTCTCCTA TTATTCCTCT GGCCTGCAGG 
     2641 ATGGGGCAGG ATCCCGCAGT GGCAGTGGCT CCCCGTCACC GGATGCATAC TCTCTCTATT 
     2701 GCTACCCTTG CACCTGGGGA GACTGTAAAG CCAGCGAGTC CTCTAGTCGC CCGCCACCTG 
     2761 GACCCCTGCC TTCTACCACC ACCCAGCCCA GCCAGGCCTC AAGAGGCCTA TCAGAGCCCC 
     2821 TGAGTGGTCG AACCCCCTCC CTCTTGGGGG CTGACACCCC AGTAGTTAAG AACTACCATA 
     2881 GCTGCCCACC TCTGTTCAAG TCCTCTCGTC CCCAGAAGTC CTTTGCTCCC TTTGGTGCAC 
     2941 TCAACCCCTT CTCTGGGCCT GCCCACCCTT CAGGTGCTCC AGCGGCCTCC TCCTCTGGGT 
     3001 CCATATCCAC CTCGGGTGTC CTGGCTACCT CTAGCCCCAC ACACTCCCCA GGCCCAGGCC 
     3061 CAGGCCCACA AGGCCAAGGT TACTCAGCTG CTCCTTCCTC CTCCTTGTCA TCCTCTGAGT 
     3121 GGCAGGAGCC AGCTCTGGAG CCCCTGGACC CATTTGAGCT GGGGCAGGCC AGTCCTCCTG 
     3181 AGCTGGAGCT AGTGCGCTGT CAGGAGCCCA GAGCTGCGGG GGCACCTGGG TCAGGACCTT 
     3241 GCCTTTCACC ACTTGGTCAG CCCAAGGCCT TTGAGCCCGA AGGTTTGGTG CTGAGGCAGG 
     3301 TCCCTGCCTC ACTGTCACCA GCTGCCTTGC AGGGGCCTGA GGCAGGAGGA ACCCTACTCT 
     3361 TTCTCACCCA AGGATGCCTG GAAGGGCCTC CTGGCAGCCC CCGGGAGGGA GCCACAGGTG 
     3421 CTGGGGTCCG GGATGCCTCC TCCTGGCAGC CACCAGCAGA CTTGTCTGCA CTCTCCCTGG 
     3481 AAGAGGTCTC CCGTAGTCTG CGCTTCATTG GGCTCTCAGA GGACGTGGTG AGCTTCTTTG 
     3541 CCCGAGAACG CATCGACGGA AGCATCTTTG TGCAGCTCAG TGAAGACATC TTGGCTGACG 
     3601 ACTTCCATCT CACCAAGCTT CAGGTCAAGA AAATCATGCA ATTCATCAAA GGTTGGAGAC 
     3661 CCAAGATCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3721 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3781 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3841 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3901 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3961 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4021 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4081 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4141 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4201 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4261 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4321 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4381 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4441 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4501 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4561 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4621 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4681 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4741 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4801 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4861 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4921 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4981 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5041 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5101 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5161 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5221 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5281 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5341 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5401 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5461 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5521 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5581 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5641 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5701 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5761 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5821 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5881 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5941 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6001 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6061 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6121 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6181 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6241 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6301 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6361 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6421 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6481 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6541 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6601 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6661 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6721 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6781 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6841 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6901 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6961 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7021 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7081 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7141 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7201 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7261 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7321 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7381 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7441 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7501 CAAAAATTTA ACGCGAATTT T
//