LOCUS       MR214728                7161 bp    DNA     circular  SYN 
DEFINITION  Adam20 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4930529F22 gene (4930529F22Rik)
ACCESSION   NM_001009548
VERSION     NM_001009548.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3318..3409 
                     /label="Myc-DDK"  
     misc_feature    3439..4024 
                     /label="PolyA signal"  
     misc_feature    3496..3515 
                     /label="Restriction Enzyme"  
     misc_feature    4300..4972 
                     /label="ColE1"  
     misc_feature    5402..6196 
                     /label="Kan/Neo"  
     misc_feature    6231..6533 
                     /label="SV40 ori"  
     misc_feature    6619..7096 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3300..3306 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3300 
                     /label="Adam20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGACAACA CAAAGGGCAT CCTCCTTTGC TGCCGCAGAG GACAACATAG 
     1081 CTATGGATAA GGCTGTGGTA TACACAAGGA TACCTCATCT GTACGTCTGG CTAGAGATAT 
     1141 TGAATATTCT TTCATCACGG CCCTTGACTG GGTATGCTCA GCACACCAGC CTCCCAGAAG 
     1201 TAGTGATACC CTTACGGGTC ACAGGCAACA GACCAATGTG GGCTATGGGA TGGCTGACCT 
     1261 ATAGCCTGCA TTTTGGGGGC CAGAAACATT TTATTCACAT AAAGGCCAAG AAGTTCTTGG 
     1321 TTTCTAGACT CTTCTCTGTA TTCACCTACA CTAAGCAAGG TGCTCTGCAC AAGGACCAGC 
     1381 CTTATGTCCA GAATGATTGC TACTACCATG GCCATATGGA TGGAGACCCA GAGTCTATGG 
     1441 TTGCTATTAC CACCTGTTAT GGAGGCTTTC AAGGAATATT ACAGATAAAT GGCACAGTCT 
     1501 ATGAAATCAA ACCCAAGAAC CTTTCTTCCA CATTTGAACA TTTGGTTCAC AAGATGGACA 
     1561 GTGAGGAGAC AGAATTACTC CCCATGAGAT GTGCATTGAC AGAAGAGATA GCAAAGCAAA 
     1621 TGAAACGTCA GCAGAATGAG AACCCCACTC TGATGCAAAA CCACTATGAG GAATGGTGGA 
     1681 CCCACAGGAG TTTTCTTGAC CTGGCATTGG TTGTTGACTG TGAGCGAATT CGTTATCATA 
     1741 ACAATAACAA ATCACACGTG TTAGTGGAAG TATTCTTGAT TATCAGTTTA ATAAATAAGA 
     1801 TTTATTTTGC ACTGGATATT GAGGTAGTTT TAATTGGACT TGAGTTGTGG AATGAAGGAA 
     1861 ATCTTGTGCC CGTGGATAGA ATGCAAATTC TTCTAGAGGA ATTTTGTGTT TGGAAGACTC 
     1921 TCAGCCTTAA TATTCGAATT CCAAATGACA TTGCACATCT TTTTGTGAAT CATTCATTTG 
     1981 GTAAATTCCT TGGTTTAGCC TATGTTGGTA CTGTCTGTCT GCCATCTCAT AATTGTGGAG 
     2041 TTGATCGTCT GTTAGGTCCT AATCTTTTTC AATTTGCACA TATTATAGCA CATGAGATTG 
     2101 GTCATAATCT GGGTATGGAG CATGATAGCA GCTCATGTAC ATGTGGAGGA ATAAATTATT 
     2161 GCTTAATGTC TTCAACGTAC AGTTTCAACC CCGAGTTCAG TAACTGTAGT TATTCTAACT 
     2221 TCTGGACAAC ATATGCTACT ACCAATTGTT TGCGCAAGGA AAAGATGTCA ATAGGCAACC 
     2281 TACAAATCCA GTTCTGTGGG AATGGAGTTG TTGACGATGG AGAACAGTGT GATTGTGGAG 
     2341 ACAGGCATAT GTGTGAAAGA GATCAATGTT GTAACTCCAG GTGTGCCCTT AATGATGGCG 
     2401 CTGCCTGTGC TTTTGGGCTT TGCTGCTTAT ATTGCCAGAT CATGCCAGCA GGCACAGTTT 
     2461 GCCGAGAAGA GGTCAATGAA TGTGATCTTC CAGAATGGTG CAACGGACAT TCACATAAGT 
     2521 GTCCTAATGA CGTTTATTTG CTTGATGGGA GTCCCTGTAG AGATGGTGGC TACTGCTATG 
     2581 AGAAGAGATG TAATAACCGA GATGAACAAT GTAAGCAAAT CTTCGGCAAA GAAGCCAGGA 
     2641 GTGCAGATCA CAGTTGCTAC AGAGAACTCA ACACCCAAGG TGACCGTTTT GGCAACTGTG 
     2701 GTATGATCAG AGATGCATAC TTAAGATGTC ATGACTCAGA CATCCTCTGT GGCAGAGTTC 
     2761 AATGTGAGAA CGTAACACGC ATTCCATTTT TGAGGGACCA TTCCACTGTA CACTGGACTC 
     2821 ACCTCAATGG TGTCACCTGT TGGGGGACTG ACTACCATTT TGGGATGACA ATCCCTGACA 
     2881 TTGGTATTGT GAAAGATGGC ACAGACTGTG GTCCAGAGCA TGTGTGTATT AATAAGAAGT 
     2941 GTGTTAGTAA GCCAATTTGG CTAGAACAGT GTTCATCCAA GACATGTACT ATGAAAGGTG 
     3001 TGTGCAACAA TTTACATCAC TGCCACTGCA ACCGGGGGTG GGACCCACCA CATTGCCTGG 
     3061 AAAGTGGCTT TGGAGGGAGT GTTGACAGTG GCCCTCCACC TGGAGAAGAG GAATCCCAGC 
     3121 AACATATGGA CCTGGTACTG ATTACTCCAA TTCTTTCCAT TGTTTCCTTA GTCTTTCTGT 
     3181 TACCTTGGCT TTTGAATAGG TATATAAGAA CATCATCAAA GTTTGAGGAA CCCACTGTTT 
     3241 CCACTTCCAA GGAGGAAGAA GAGAGCCATG TGGCAAGTAA TGAGTCAGAA GCGCAACAGG 
     3301 TTGAAACAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3361 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3421 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3481 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3541 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3601 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3661 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3721 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3781 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     3841 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     3901 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     3961 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4021 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4081 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4141 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4201 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4261 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4321 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4381 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4441 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4501 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4561 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4621 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4681 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4741 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4801 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4861 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4921 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     4981 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5041 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5101 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5161 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5221 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5281 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5341 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5401 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5461 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5521 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5581 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5641 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5701 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5761 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     5821 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     5881 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     5941 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6001 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6061 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6121 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6181 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6241 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6301 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6361 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6421 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6481 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6541 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6601 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6661 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6721 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6781 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     6841 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     6901 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     6961 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7021 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7081 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7141 CAAAAATTTA ACGCGAATTT T
//