LOCUS       MR214512                8391 bp    DNA     circular  SYN 
DEFINITION  Nrde2 (Myc-DDK-tagged) - Mouse cDNA sequence BC002230 (BC002230)
ACCESSION   NM_183155
VERSION     NM_183155.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4546..4637 
                     /label="Myc-DDK"  
     misc_feature    4667..5252 
                     /label="PolyA signal"  
     misc_feature    4724..4743 
                     /label="Restriction Enzyme"  
     misc_feature    5528..6200 
                     /label="ColE1"  
     misc_feature    6630..7424 
                     /label="Kan/Neo"  
     misc_feature    7459..7761 
                     /label="SV40 ori"  
     misc_feature    7847..8324 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4521..4528 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4521 
                     /label="Nrde2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGTTC CCGGCTTTTG CGGACGTAAG TGAGGCCTCC GGTGATGGGG 
     1081 CATTCAGGAA AGAGTTAGAT TGGCTGAGCA ACCCAAGCTT TCGTGTTGGA AATCTAACTT 
     1141 CTCTGAGCCG ACAAACTGAA GAGGTCACAG CCCTTGCTTC TGAAGGGTCA CCACCGCCAA 
     1201 GGAGTCCTCT GAAGTCAGAG CTCTCAGGTG AGAGCAACAC TAGCGAGAAG CTCGCACAGA 
     1261 CAAGCAGGAA GAAGAAGAAG GAGAAAAAGA AGAGACGGAA GCATCAGCAC CACAGGAAAA 
     1321 CCAAGAGGAG GCACGAGCAG TTGAGCAGCA GCGGCTCTGA GTCAGACACA GAGGCTGGGA 
     1381 AGGACAGGGC TTCCCGGAGC ATCAGGGACG ACCAGAAGGA AGCCGAGAAA CCCTGTCAGG 
     1441 GAAGCAATGC GGCTGCTGCT GTTGCTGCTG CTGCTGGACA TCGGTCCATT TGGCTGGAAG 
     1501 ATATTCACGA TCTGACCGAC GTCTTCAGGA CTGATAAGAA GCCAGATCCT GCCAACTGGG 
     1561 AGTATAAATC TCTGTATCGT GGGGATATAG CAAGGTACAA GAGGAAGGGA GACTCCTGCC 
     1621 TGGGTATCAA TCCTAAGAAA CAGTGTATAT CTTGGGAAGG GGCTTCTGCA GCAAAGAAGC 
     1681 ACTCACACAG GCACCTGGAG CGCTATTTTA CCAAGAAGAA TGTGGGGTTA ATGAGAACAG 
     1741 AGGGCATTGC TGTGTGCAGT AACCCCGAGC CTGCCTCCTC CGAGCCCGTC ACATTCATCC 
     1801 CAGTGAAGGA CTCTGCTGAG GCAGCTACTC CTGTTACATC ATGGCTGAAT CCTCTGGGGA 
     1861 TTTATGATCA GTCTACCACA CAGTGGTTAC AAGGACAGGG TCCAGCAGAG CAAGAGTCAA 
     1921 AGCAGCCGGA CTCGCAACAG GACAGGGAGA ATGCTGCTCT CAAGGCCAGA GTGGAAGAGT 
     1981 TTAACAGAAG GGTTCGGGAG AATCCTTGGG ACACGCAACT ATGGATGGCG TTTGTGGCTT 
     2041 TCCAGGACGA GGTCATGAGG AGTCCTGGCA TCTATGCCCT TGGGGAAGGA GAACAGGAGA 
     2101 AGCACAGGAA GTCCCTGAAG CTCCTCCTGG AGAAGAAGCT GGCTGTGCTG GAGCGGGCCA 
     2161 TCGAGAGCAA CCCAGGCAGC GTGGAGCTGA AGCTTGCCAA GCTGCAGCTG TGCTCAGAGT 
     2221 TCTGGGAGCC CAGTGCGCTG GCCAAGGAGT GGCAGAAGCT CCTCTTCCTG CACCCCAACA 
     2281 ACACAAGCCT GTGGCAGCGC TATCTCTCCT TTTGCCAGAG CCAGTTTGGT ACCTTTTCTG 
     2341 TGTCCAAACT CCACAGTCTG TATGGCAAGT GCTTGAGTAC CTTGTCTGCT GTTAAAGATG 
     2401 GCAGCATGCT ATCCCACCCT GTGTTGCCGG GCACCGAGGA GGCCATGTTT GGGCTCTTTC 
     2461 TTCAGCAGTG CCACTTTCTA CGGCAGGCTG GCCACTCAGA GAAGGTCATC TCTTTGTTCC 
     2521 AGGCCATGGT TGACTTCACC TTCTTCAAGC CTGACAGTGT GAAAGAGCTG CCTACTAAAG 
     2581 TACAGGTGGA ATTCTTTGAG CCCTTCTGGG ACAGCGGAGA ACCACGCGTT GGGGAGAAGG 
     2641 GAGCCCGAGG CTGGCGGGCG TGGATGCACC AACAGGAGCG AGGTGGCTGG GTGCTCATCA 
     2701 CCCCAGATGA GGATGACGAG GAGCCCGAAG AGGAGGACCA GGAGATAAAA GACAAGACCC 
     2761 TGCCCCGGTG GCAGATCTGG CTTGCTGTTG AGCGTTCTCG AGACCAGAGG CACTGGCGTC 
     2821 CCTGGCGCCC TGATAAGACC AAGAAGCAAA CGGAGGAAGA CTGTGAGGAC CCTGAGAGAC 
     2881 AGGTGCTGTT TGATGACATT GGGCAATCTT TGATCAGACT TTCCAGCCCA GACCTTCAGT 
     2941 TCCAGCTCAT CCAGGCCTTT CTGCAGTTCC TGGGCGTGCC TTCCGGCTTC CTGCCCCCAG 
     3001 CCTCCTGCCT TTACCTGGCC ATGGACGAGA GCAGCATCTT TGAGAGTGAA CTTTATGATG 
     3061 AAAAGCCCCT GACTTACTTC AACCCTTCCT TTTCGGGCAT TAGCTGTGTC GGCTCCATGG 
     3121 AACAGTTGGG TCGCCCTCGC TGGACCAAAG GTCACAACCG AGAGGGCGAG GAGTTTGTCC 
     3181 GCAATGTCTT CCACCTGGTG CTGCCTTTGC TCGCAGGCAA GCAGAAGTCC CAGCTCAGCC 
     3241 TCTCCTGGTT ACGGTACGAG ATTGCAAAGG TCATTTGGTG TCTACACACT AAAAAGAAGC 
     3301 GATTGAAGTC TCAGGGAAAG AGCTGCAAAA AACTAGCCAA GAATCTCCTC AAGGAGCCAG 
     3361 AAAACCGCAA CAACTTCTGC CTCTGGAAGC AGTATGCACA CCTGGAGTGG TTGCTCGGTA 
     3421 ACACAGAGGA TGCCAGAAAA GTGTTCGACA CAGCACTCAG CATGGCAGGC AGCAGCGAGC 
     3481 TGAAGGACCG TGAACTCTGC GAGCTCAGTC TGCTCTATGC CGAGCTGGAG ATGGAACTGT 
     3541 CGCCGGACTC GAGAGGAGCC ACCACAGGCC GAGCGGTCCA CATATTAACA CGGCTGACGG 
     3601 AGAGCAGTCC CTACGGGCCC TACACGGGGC AGGTCTCGTC CACCCAGGTG CTGAAAGCTC 
     3661 GGAAGGCTTA CGAGCTCGCG CTGCAGGACT GCCTGGGCCA GAGCTGTGCC TCCAGCCCAG 
     3721 CTCCTGCAGA GGCCTTGGAC TGCCTGGGTA GCTTGGTCCG ATGCTTTATG CTCTTCCAGT 
     3781 ATTTGACTGT GGGCATTGAT GCTGCTGTGC AGATATACGG AAGGGTGTTT GCCAAGCTGA 
     3841 AGGGCTCTGC TCGCTTAGAA GACCCAGGGC CAGAGGACAG CACCAGCTCC CAGAGTTTGA 
     3901 CCAATGTGCT CGAGGCTGTC AGCATGATGC ACACAAGCCT GCTGCGATTC CACATGAACG 
     3961 TTTGTGTTTA CCCTCTGGCT CCACTCCGAG AGACCCTCTC CGATGCTTTA AAGCTGTATC 
     4021 CGGGCAACCA GGTTCTTTGG AGGGCTTATG TACAGATTCA GAATAAGTCC CACAGTGCTA 
     4081 ACAAGACCAG AAGGTTCTTT GACACAGTTA CTAGGTCTGC CAAACATTTG GAGCCTTGGC 
     4141 TATTTGCAAT CGAAGCTGAG AAGCTGAGGA AAAAGCTAGT GGAATCTGTT CAGAGGGTAG 
     4201 GAGGCAGAGA GGTCCATGCA ACAATCCCCG AGACTGGCCT TACGCATCGC ATCAGAGCTT 
     4261 TGTTTGAAAA TGCGATTCGG AGCGACAAGG GCAACCAGTG TCCCCTACTG TGGAGGATGT 
     4321 ATTTGAATTT TTTGGTTTCC TTAGGAAACA AAGAAAGAAG CAAAGGCGTG TTCTACAAGG 
     4381 CCCTCCAGAG CTGTCCTTGG GCAAAGGTGC TATACATGGA CGCCATGGAG TATTTCCCAG 
     4441 ACGAGCTGCA GGAGATCCTG GACGTGATGA CCGAGAAGGA GCTCCGTGTG CGCCTGCCCC 
     4501 TTGAGGAGCT GGAGCTGCTG CTGGAGGATA GCGGACCGAC GCGTACGCGG CCGCTCGAGC 
     4561 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4621 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4681 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4741 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4801 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4861 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4921 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4981 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5041 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5101 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5161 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5221 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5281 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5341 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5401 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5461 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5521 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5581 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5641 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5701 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5761 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5821 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5881 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5941 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6001 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6061 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6121 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6181 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6241 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6301 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6361 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6421 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6481 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6541 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6601 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6661 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6721 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6781 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6841 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6901 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6961 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7021 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7081 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7141 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7201 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7261 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7321 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7381 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7441 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7501 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7561 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7621 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7741 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7801 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7861 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7921 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7981 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8041 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8101 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8161 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8221 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8281 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8341 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//