LOCUS       MR213908                7218 bp    DNA     circular  SYN 
DEFINITION  Pcdhb8 (Myc-DDK-tagged) - Mouse protocadherin beta 8 (Pcdhb8)
ACCESSION   NM_053133
VERSION     NM_053133.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3375..3466 
                     /label="Myc-DDK"  
     misc_feature    3496..4081 
                     /label="PolyA signal"  
     misc_feature    3553..3572 
                     /label="Restriction Enzyme"  
     misc_feature    4357..5029 
                     /label="ColE1"  
     misc_feature    5459..6253 
                     /label="Kan/Neo"  
     misc_feature    6288..6590 
                     /label="SV40 ori"  
     misc_feature    6676..7153 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3357..3363 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3357 
                     /label="Pcdhb8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGACAGCG CTAACAAAAA CGCCAGAGAA AAGGCAAGTC ATTTTCCTTG 
     1081 CTATATTGTT GCTTTTGTGG GAGGCTAGTT CTGAGGCAAT TAGCTATTCC ATGCCAGAAG 
     1141 AAACGGAGAG TGGCTACTTG GTGGCTAACC TGGCACAAGA TCTGGGGCTC AGGGTTGGGG 
     1201 AACTGACCAC TAGAGGGGCA CGAATCCATC ACAATGGTAA CAAAGAGCTC TTGCAGCTTG 
     1261 ATGCAGAGAG AGGGAATTTG CTCCTGAAGG AAAAACCAGA TCGCGAAGCA CTGTGTGGGG 
     1321 CGACAGAACC CTGTGTGCTG CACTTCCAGA TCATACTAGA AAACCCTGTG CAGTTCTTTC 
     1381 AAACAGACTT GCAGTTCACA GATATAAACG ACCATTTCCC AGAGTTCCCT GACACAGAAA 
     1441 TGCTCCTAAA AATTCAAGAA ATTGCCCAGC CAGGGACTGT GTTTCCTCTG AAGGCAGCTC 
     1501 AGGACCCTGA CATAGGGAGC AACGCTGTTC AGAACTACAC AGTCAGTCCC AACCTCCATT 
     1561 TCCATGTCGT TACTCTCAGT CGCTCAGATG ACAGGAAATA CCCAGAGCTG GTGCTGGACA 
     1621 GAGCCCTGGA CAGGGAGGAG CAGCCTGAAC TCACTTTAAT CCTCACTGCT CTGGATGGCG 
     1681 GAGCTCCACC CAAGTCTGGG ACAACCACTG TTCGGATTGA AGTCGTGGAC ATCAATGATA 
     1741 ATGCCCCCCA GTTCTTACAG TCACTCTATG CGGTGGAGGT CCCTGAGAAC AGTCCCCTCA 
     1801 ATGCCTTAGT TGTCACTGTC TCTGCCAGGG ATTTAGATGC TGGGATACAT GGCAATGTAG 
     1861 CCTACTCTCT GTTTCAAGGC GGGGGAGGTC CTCAGCCATT TGTAATAGAT GAAATCACAG 
     1921 GAGAAATTCG ACTTAAAGGG GCATTGGATT TTGAAGCAAC TTCATACTAT ACCATGGAAA 
     1981 TTGTAGCCAC AGACAGTGGT GGTCTTTCAG GAAAATGCAC TGTAGCTATC CAGGTGTTGG 
     2041 ATGTGAACGA CAATGCCCCT AAACTCACCA TATCTTCGCT CACTAGTTCC ATCCCAGAAA 
     2101 ATGCTCCGGA GGCTGTAGTT GCTGTTTTCA GTGTCTCTGA CCCAGATTCG GGGGACAATG 
     2161 GAAGGATGGT GTGTTCAATT CAGAACGGAC TTCCATTTCT TTTAAAACCC ACATTCAAGA 
     2221 ACTTTTACAC CTTAGTCACG GAGAGACCCC TGGATAGAGA GAGCAACGCT GAGTACAACA 
     2281 TCACAATCAC GGTCTCTGAC CTGGGCACAC CCAGGCTCAC AACCCAGCAC ACCATAACAG 
     2341 TGCAAGTGTC CGACATCAAC GATAATGCTC CTGCCTTCAC TCAAACCTCT TACACCTTGT 
     2401 TTGTTCACGA GAACAACAGC CCTGCCCTGC ACATAGGCAC CATCAGCGCC ACAGACTCAG 
     2461 ACTCTGGCTC CAATGGCCTT ATTATCTACT CGTTGCTGCC GCCCCATGAC CAGCAGCTGG 
     2521 GCCTTGCCTC GCTGATCTCC ATCAACTCAG ACAACGGGCA GCTGTTTGCG CTCAGGGCGC 
     2581 TGGACTACGA GGCCCTGCAG GCCTTCGAGT TCCACGTGGG CGCCACAGAC AGAGGCTCGC 
     2641 CCGCGCTCAG CTCAGAGGCT CTGGTGCGCG TAGTGGTGCT GGATGACAAT GACAATGCGC 
     2701 CCTTCGTGCT CTACCCACTG CAAAACGCTT CTGCGCCCTG CACTGAGCTG CTGCCCAGGG 
     2761 CTGCGGAGCC TGGCTACCTG ATCACCAAGG TGGTGGCAGT GGACCGCGAC TCTGGCCAGA 
     2821 ATGCCTGGCT GTCATTCCAG CTGCTCAAGG CCACAGAGCC CGGGCTGTTC AGCGTGTGGG 
     2881 CGCACAATGG CGAAGTGCGC ACCACCAGGC TGCTGAGCGA GCGAGATGCA CCCAAGCACA 
     2941 GGCTGCTGCT GCTGGTCAAG GACAATGGAG AGCCTCTGCG CTCTGCTAGT GTCATGCTGC 
     3001 AGGTGCTAGT GGTGGATGGC TTCTCTCAGC CCTACCTGCC TCTGCCAGAG GTGGCGCTCA 
     3061 ACCCCACACA GGAAGAAGAC ATGCTCACTC TCTATCTGGT TATTGCCTTG GCTTCTGTGT 
     3121 CTTCACTTTT CCTCTTGTCT GTGCTGCTGT TTGTGGGGGT GAAGTTGTGC AAGAAGGCCA 
     3181 GGGAGGCTTC TCTGGCTGAC TGCTCTATTC CTGAGGGACA CTTTCCTAGC CACTTGGTGG 
     3241 ATGTCAGTGG TGCGGGGACC TTATCCCAGA GTTACCATTA TGAGGTCTGT CTGACTGAGG 
     3301 ACTCTGGGAC CTCAGATTTC AAGTTCATGA ACCCCATTAT TCCTAGTAGT CTCCTTCAGG 
     3361 ACTCTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3421 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3481 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     3541 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     3601 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     3661 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     3721 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     3781 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     3841 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     3901 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     3961 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4021 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4081 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4141 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4201 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4261 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4321 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4381 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4441 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4501 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     4561 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     4621 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     4681 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     4741 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     4801 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     4861 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     4921 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     4981 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5041 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5101 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5161 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5221 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5281 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5341 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5401 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5461 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     5521 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     5581 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     5641 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     5701 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     5761 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     5821 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     5881 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     5941 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6001 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6061 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6121 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6181 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6241 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6301 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6361 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6421 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6481 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6541 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6601 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     6661 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     6721 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     6781 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     6841 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     6901 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     6961 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7021 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7081 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7141 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7201 AAATTTAACG CGAATTTT
//