LOCUS       MR213906                8745 bp    DNA     circular  SYN 
DEFINITION  Sympk (Myc-DDK-tagged) - Mouse symplekin (Sympk)
ACCESSION   NM_026605
VERSION     NM_026605.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4902..4993 
                     /label="Myc-DDK"  
     misc_feature    5023..5608 
                     /label="PolyA signal"  
     misc_feature    5080..5099 
                     /label="Restriction Enzyme"  
     misc_feature    5884..6556 
                     /label="ColE1"  
     misc_feature    6986..7780 
                     /label="Kan/Neo"  
     misc_feature    7815..8117 
                     /label="SV40 ori"  
     misc_feature    8203..8680 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4884..4890 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4884 
                     /label="Sympk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAAGCAGC AGCGGGGACA GTGTCACCCG CCGGAGTGTG GCATCACAGT 
     1081 TTTTCACACA AGAAGAAGGG CCAAGCATCG ATGGCATGAC CACTTCTGAG AGGGTGGTAG 
     1141 ATCTTCTGAA CCAGGCTGCA TTGATCACCA ATGACTCCAA GATCACAGTG CTCAAACAGG 
     1201 TCCAAGAGCT GATCATCAAC AAGGACCCCA CCCTGCTTGA CAACTTCCTG GATGAGATCA 
     1261 TCGCTTTCCA AGCAGACAAA TCCATTGAAG TGCGCAAGTT TGTCATTGGC TTCATTGAGG 
     1321 AGGCATGCAA GCGGGACATT GAGCTGCTGC TAAAACTGAT CGCCAACCTC AACATGCTAT 
     1381 TGCGGGATGA AAATGTGAAC GTGGTTAAGA AGGCCATCCT CACCATGACC CAGCTCTACA 
     1441 AGGTGGCCCT ACAGTGGATG GTGAAGTCTC GAGTCATCAG TGATCTCCAG GAGGCCTGCT 
     1501 GGGACATGGT GTCCTCTATG GCTGGGGAAA TCATCCTGCT TTTGGATTCT GACAACGATG 
     1561 GCATCCGTAC ACATGCCATC AAGTTTGTGG AGGGCCTCAT TGTCACCCTG TCACCCCGCA 
     1621 TGGCTGACTC AGAGGTCCCA CGACGCCAGG AGCATGACAT CAGTCTGGAC CGCATTCCTC 
     1681 GAGATCACCC CTACATCCAG TACAATGTCC TATGGGAAGA AGGCAAGGCA GCTGTGGAGC 
     1741 AGCTGCTGAA GTTCATGGTG CACCCCGCCA TCTCCTCCAT CAACCTGACC ACAGCGCTCG 
     1801 GCTCCCTTGC CAACATCGCC CGCCAGAGAC CCATGTTCAT GTCCGAGGTG ATCCAGGCCT 
     1861 ATGAGACTCT GCATGCCAAC CTGCCCCCAA CGCTGGCCAA GTCCCAGGTG AGCAGTGTGC 
     1921 GGAAGAACCT TAAGCTGCAC TTGCTCAGTG TGCTCAAGCA CCCTGCCTCC TTGGAGTTCC 
     1981 AGGCCCAGAT CACCACCCTG CTGGTGGATC TGGGCACGCC CCAGGCTGAG ATCGCCCGAA 
     2041 ACATGCCTAG CAGCAAGGAC TCCCGCAAGC GGCCCCGGGA CGATACAGAT TCCACGCTCA 
     2101 AGAAGATGAA GCTGGAGCCC AACTTGGGAG AAGACGATGA GGACAAAGAC TTGGAGCCTG 
     2161 GCCCATCAGG GACGTCAAAG GCGTCAGCCC AGATCTCAGG CCAGTCAGAC ACGGACATCA 
     2221 CTGCTGAGTT CTTGCAGCCT CTGCTGACAC CTGACAATGT GGCCAATCTG GTCCTCATCA 
     2281 GCATGGTGTA CCTGCCTGAG ACCATGCCTG CCTCCTTCCA AGCCATCTAC ACCCCTGTGG 
     2341 AGTCAGCAGG CACTGAAGCC CAGATCAAGC ACCTGGCTCG ACTCATGGCC ACACAGATGA 
     2401 CAGCTGCAGG ACTGGGTCCT GGTGTGGAAC AGACGAAACA GTGCAAGGAG GAGCCCAAGG 
     2461 AGGAGAAGGT GGTAAAGCCG GAGAGCGTCC TGATCAAGCG GCGCCTGTCG GTGCAGGGCC 
     2521 AGGCCATCTC TGTGGTAGGC TCTCAGAGCA CCATGTCTCC CTTGGAGGAG GAGGTACCCC 
     2581 AGGCCAAGAG GAGGCCAGAA CCCATCATCC CTGTCACGCA GCCACGGCTG GCAGGCGCAG 
     2641 GTGGGCGCAA GAAGATCTTC CGTCTGAGCG ATGTGCTGAA GCCCCTGACA GATGCCCAGG 
     2701 TGGAAGCCAT GAAGCTAGGA GCTGTGAAGC GGATCCTGAG GGCTGAGAAG GCTGTAGCCT 
     2761 GCAGTGGGGC AGCCCAGGTG CGCATCAAGA TCCTGGCCAG CCTCGTAACA CAGTTCGACT 
     2821 CCGGATTCAA GGCAGAGGTG CTGTCCTTTA TCCTGGAAGA TGTCCGGGCC CGTCTGGACC 
     2881 TGGCTTTTGC CTGGCTCTAC CAGGAATATA ATGCCTACCT GGCAGCAGGG ACCTCAGGCA 
     2941 CTCTGGACAA ATATGAGGAT TGCCTCATCT GCCTGCTCTC TGGACTACAA GAGAAACCAG 
     3001 ACCAGAAAGA TGGGATCTTT ACCAAGGTGG TTTTGGAGGC CCCACTGATC ACTGAGAGTG 
     3061 CCTTGGAGGT GATACGGAAG TACTGTGAGG ATGAGAGTCG TGCCTACCTG GGCATGTCGA 
     3121 CACTTGGAGA CCTGATCTTC AAGCGCCCTT CTCGCCAGTT CCAGTATCTG CACGTTCTTC 
     3181 TTGACCTCAG CTCTCACGAG AAGGACAGGG TGCGCTCCCA GGCCTTGCTC TTCATTAAGC 
     3241 GAATGTATGA GAAGGAGCAG CTTCGAGAGT ATGTGGAGAA ATTTGCCCTC AACTACCTGC 
     3301 AGCTCCTGGT CCACCCCAAC CCACCCTCGG TGCTGTTTGG AGCCGACAAG GACACAGAGG 
     3361 TGGCCGCACC CTGGACAGAG GAAACAGTGA AGCAGTGTTT GTATCTGTAT CTGGCTCTCC 
     3421 TGCCTCAGAA CCACAAGCTG ATCCATGAGC TGGCAGCCGT GTACACTGAA GCCATCGCTG 
     3481 ACATCAAGCG GACAGTGCTG AGGGTCATTG AGCAGCCGAT CCGAGGGATG GGCATGAACT 
     3541 CTCCAGAGCT GCTGCTGCTG GTAGAAAACT GTCCCAAGGG GGCAGAGACG CTGGTCACCC 
     3601 GCTGCCTGCA CAGCCTCACC GACAAAGTGC CACCATCCCC AGAACTGGTG AAGCGGGTCC 
     3661 GAGACCTGTA CCACAAACGG CTGCCTGATG TGCGCTTCCT CATCCCCGTG CTCAATGGGT 
     3721 TGGAGAAGAA AGAGGTGATC CAGGCGCTGC CCAAGCTCAT CAAACTCAAC CCCATTGTGG 
     3781 TGAAGGAAGT CTTCAACCGC CTGCTAGGCA CTCAGCACGG TGAGGGGAAC TCGGCCCTGT 
     3841 CCCCCCTGAA TCCAGGAGAG CTGCTGATTG CATTGCACAA TATTGACTCT GTGAAGTGTG 
     3901 ACATGAAGTC CATCATCAAA GCCACCAACC TGTGTTTTGC GGAGCGGAAT GTGTACACGT 
     3961 CGGAAGTGCT AGCTGTGGTG ATGCAGCAGC TGATGGAGCA AAGCCCTCTG CCCATGCTGC 
     4021 TCATGCGGAC CGTCATCCAG TCCCTCACCA TGTACCCCCG CCTGGGGGGC TTCGTCATGA 
     4081 ACATCCTGGC CCGACTCATC ATGAAGCAGG TATGGAAGTA CCCCAAGGTA TGGGAGGGCT 
     4141 TCATCAAGTG CTGCCAGCGC ACCAAGCCAC AGAGTTTCCA GGTCATCCTG CAGCTACCTC 
     4201 CACAGCAGCT TGGCGCAGTC TTTGACAAGT GCCCTGAGCT CCGGGAGCCA CTGTTGGCCC 
     4261 ATGTGCGCTC CTTCACTCCC CACCAGCAAG CACACATCCC CAACTCCATC ATGACCATCC 
     4321 TGGAGGCCAC TGGAAAGCAG GAGCCCGAAG TCAAGGAAGC ACCTTCAGGG CCCCTGGAAG 
     4381 AGGATGACCT GGAGCCCTTG GCTCTGGCTT TGGCCCCGGC TCCGGCCCCG GCCCCGGCCC 
     4441 CGGCCCCGGC CCCGGCCCCT GCCCCACGGC CCCCTCAGGA CCTCATCGGC CTGCGATTGG 
     4501 CTCAGGAGAA GGCCCTGAAG AGGCAGCTGG AGGAGGAACA GAAGCAGAAG CCCACAGGCA 
     4561 TCGGAGCGCC CGCTGCCTGT GTGTCCTCAA CGCCCTCGGT GCCAGCAGCA GCCCGAGCTG 
     4621 GGCCCACCCC AGCTGAGGAG GTCATGGAAT ACCGGGAGGA GGGTCCCGAA TGCGAGACCC 
     4681 CGGCCATCTT CATCAGCATG GACGACGACT CGGGGCTGGC TGAAACCACA CTGCTGGACT 
     4741 CTAGTCTCGA GGGGCCCCTG CCTAAGGAGG CAGCAGCAGT CGGGTCGAGC TCCAAGGATG 
     4801 AGCGGAGCCC CCAGAACCTC AGCCATGCTG TGGAAGAAGC CTTGAAGACC TCCAGCCCAG 
     4861 AAACCAGGGA ACCCGAGAGC AAGGGGAACA GCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4921 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4981 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5041 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5101 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5161 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5221 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5281 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5341 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5401 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5461 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5521 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5581 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5641 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5701 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5761 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5821 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5881 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5941 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6001 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6061 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6121 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6181 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6241 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6301 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6361 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6421 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6481 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6541 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6601 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6661 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6721 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6781 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6841 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6901 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6961 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7021 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7081 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7141 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7201 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7261 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7321 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7381 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7441 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7501 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7561 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7621 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7681 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7741 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7801 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7861 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7921 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7981 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8161 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8221 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8281 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8341 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8401 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8461 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8521 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8581 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8641 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8701 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//