LOCUS       MR213825                8688 bp    DNA     circular  SYN 
DEFINITION  Plekha5 (Myc-DDK-tagged) - Mouse pleckstrin homology domain containing, family A member 5 (Plekha5)
ACCESSION   NM_144920
VERSION     NM_144920.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4845..4936 
                     /label="Myc-DDK"  
     misc_feature    4966..5551 
                     /label="PolyA signal"  
     misc_feature    5023..5042 
                     /label="Restriction Enzyme"  
     misc_feature    5827..6499 
                     /label="ColE1"  
     misc_feature    6929..7723 
                     /label="Kan/Neo"  
     misc_feature    7758..8060 
                     /label="SV40 ori"  
     misc_feature    8146..8623 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4827..4833 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4827 
                     /label="Plekha5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCGAC CTGAACCTGG AGTGGATCTG CTCGCTGCCG CGCTCCTGGA 
     1081 CGTACGGGAT CACTAGGGGC GGCCGCGTCT TCTTCATCAA CGAGGAGGCC AAGAGCACCA 
     1141 CCTGGCTACA CCCGGTCACC GGCGAGGCCG TGGTCACCGG GCACCGGCGG CAGAGCACAG 
     1201 ATTTGCCCAC TGGCTGGGAA GAAGCATATA CGTTCGAAGG TGCAAGATAC TATATAAACC 
     1261 ACAATGAAAG GAAAGTGACC TGCAAGCATC CGGTTACAGG ACAGCCATCC CAAGACAACT 
     1321 GTATCTTTGT TGTGAATGAT CAGACTGTTG CAACCATGAC GTCCGAAGAC AAGAAGGAAC 
     1381 GACCCATAAG TATGATAAAT GAAGCTTCCA ATTACAACAT GGCTTCAGAT TATGCAGTGC 
     1441 ATCCCATGAG CCCTGTGGGC AGGACCTCGC GGGCCTCCAA AAAGGTTCAT AATTTTGGGA 
     1501 AAAGGTCCAA TTCAATTAAA AGGAACCCTA ATGCACCTGT GGTCCGGCGT GGCTGGCTGT 
     1561 ATAAGCAGGA CAGCACCGGC ATGAAGCTGT GGAAGAAGCG CTGGTTTGTG CTCTCTGACC 
     1621 TTTGCCTTTT CTATTACCGA GATGAGAAGG AGGAGGGTAT TTTGGGAAGT ATCCTGTTGC 
     1681 CTAGTTTTCA GATAGCCATG CTCACTGCTG AGGACCACAT CAATCGCAAA TATGCTTTTA 
     1741 AGGCAGCCCA CCCAAACATG CGGACCTATT ATTTCTGCAC CGATACAGGA AAGGAGATGG 
     1801 AGCTGTGGAT GAAGGCCATG TTAGATGCTG CTCTGGTGCA GACAGAACCT GTGAAAAGAA 
     1861 TTACCTTTAA TTTCCGAGTG GACAAGATTA CAACTGACAG TGCATCAACC AAAGAAACCA 
     1921 ATAACATTCC CAACCACAGA GTTCTCATTA GACCCGAGGT CCAAAACCAT CAGAAGAATA 
     1981 AGGAAATCAG CAAAATTGAA GAAAAAAGGG CCTTAGAAGC TGAAAGATAC GGATTTCAGA 
     2041 AGGACGGGCA AGATAGACCA TTAACAAAAA TTAACAGTGT GAAGTTGAAC TCCCTGCTAT 
     2101 CAGAATATGA AAGCGGGCCA GACTGCCCAC CTCAGAATGT ACACTACAGA CCCATCAATG 
     2161 TGAATAGTTC TGACGGCAAA GCGGTGAATG TCAGCTTGGC AGATGTCCGG GGTGGGAGTC 
     2221 ACCCAAATGC AGGGCCCCTA GCCACAGAGG CCGACCGAGT CATTCAGAGA ACGAATTCAA 
     2281 TGCAACAGCT AGAGCAGTGG ATTAAAGTCC AGAAAGGACG GGGTCTTGAA GAAGAGCCCA 
     2341 GAGGAGTAAT TTCTTATCAA ACATTGCCAA GAAATATGCC AAGCCACAGA GCCCAGATCC 
     2401 TGGCCCGCTG CCCTGAAGGT TACCGGACAC TCCCGAGAAA CAGCAAGACC AGACCTGAGA 
     2461 GTATCTGCAG CGTAACCTCT TCTGGTCATG AGAAGACAGG GCCGGGAGCA GAAGAGAAGC 
     2521 GGCGATCCAT GAGAGATGAC ACCATGTGGC AGCTGTACGA GTGGCAGCAG CGCCAGTTTT 
     2581 ACCACAAGCA GAGCACCCTC CCTCGGCACG GCTGCCTGAG CAGTCCCAAA GCCATGGTGC 
     2641 AGGTGTCTGA CCAGACGATG CACTCCATCC CCACGTCACC TTCCCATGGG TCAGCAGCTG 
     2701 CTTACCAGGG CTTCTCCCCT CAGCGGACGT ACAGGTCAGA AGTGACCTCG CCCATCCAGA 
     2761 GAGGAGATGT GACGATAGAC CGCAGGCACA GGCCTCATCA CCCTAAGCAT GTCTATGTAG 
     2821 CTGACAGAAG GTCAATGCCA GCTGGCTTAA CTTTACAGGC TGTTAGTCCC CAGAGCCTCC 
     2881 AAGGCAGAAC GCCAGAGGAG CTCACACTGC TGCTAATAAA GCTGAGACGG CAGCAGGCAG 
     2941 AGCTGAGTAG TGTCCGGGAG CACACCTTAG CACAGCTCAT GCAGCTGAAA CTTGAGGCCC 
     3001 ACAGCCCAAA GAATGAGATT CTTTCACATC ATCTCCAAAG GAACACCATA TATTTGGATC 
     3061 ATCAGATGAA AGAAAATGAA CCTATTATCA CCATGGTTCA CACAATGATT GAGAACTCGG 
     3121 CGCTAAGACC ACAACTGTAC CAACAATTCT TAAGACAGAA GAACAAGATA AGTCTCTATT 
     3181 GTCTGTCACA AGATGAATGT AGAGGCACAT TATACAAATA CAGACCTGAA GAAGCAGGTA 
     3241 TTGATGCCAA ATTAAGCAGA TTGTGTGAAC AGGATAAAGT GGTCCGAGCG CTGGAGGAGA 
     3301 AGCTGCAGCA GCTGCACAAG GAGAAATACA CGCTTGAGCA AGCCTTGCTG TCAGCCAGCC 
     3361 AAGAGATAGA GATGAATGCT GATAACCCAG CTGCCATTCA AACTGTGGTG TTGCAGAGGG 
     3421 ATGACCTGCA AAACGGACTG CTGAGCACGT GTCGGGAACT CTCCCGAGCC ACTGCTGAGC 
     3481 TGGAACGAGC CTGGAGAGAA TATGACAAGT TGGAATATGA TGTGACTGTT ACCAGGGACC 
     3541 AGATGCAGGG TCAGCTCGAC CGCCTTGGAG AAGTGCAGAG TGAGTCGGCA GGAATTCAAC 
     3601 GGGCACAGAT TCAAAAGGAG CTCTGGAGAA TTCAAGATGT CATGGAGGGG TTGAGCAAAC 
     3661 ACAAACAGCA AAGAGGCTCT TCAGAAACAG GTCTGGCAGG ATCAAAGCCT TTCTCATCAG 
     3721 TTAAGTACAA GAGCGAGGAA GAGGAAGTAG TCCCACCTCG GCCTCCACTT CCTCGGTCTT 
     3781 ATGACTTTAC AGAGCAGCCT CCCATAATCC CCCCTCTGCC CAGTGATAGC AGCTCCTTGC 
     3841 TCTGTTATAG CAGGGGCCCC GTGCATCTGC CTGAAGACAA GAAGATTCAT CAAGTTCAAG 
     3901 GATATCCAAG AAATGGATCT CACTGTGGTC CAGATTATAG ACTCTACAAG AGTGAGCCAG 
     3961 AGTTAACCAC AGTGGCAGAA GTCGATGAAT CTAACGGAGA AGAAAAGTCA GAGCCAGTTT 
     4021 CTGAGACAGA AGCTCCAGTC GTTAAAGGTT CCCATTTTCC AGTCGGAGTT CCTCTGAGAA 
     4081 CAAAGTCACC CACACCCGAG TCCTCCACGA TAGCTTCCTA TGTGACCTTG AGGAAGACTA 
     4141 AGAAGATGGT GGAGCTAAGA ACGGAAAGAC CAAGAAGTGC CGTGGAGCAG CTGTGTCTGG 
     4201 CTGAGAGTGC TCGACCTAGA ATGACTGTGG AAGAGCAGCT AGAGAGGATC AGGAGACACC 
     4261 AGCAGGCCTG CCTGAGGGAG AAGAAGAAGG GGCTGAGTGT CCTCGGTGCT TCCGACCCGT 
     4321 CAGACGTGAG GGACAGTCCA CTCAGGCTTA CTCAGACTCT AAGGAGGGAT GATAACGTTA 
     4381 AGGAGCTAGA CACCGTCCAC AGAGAAAATG ATGTAAAGCC AGACTACGAG ACTCCAGCAG 
     4441 CACAGTGTGC TCACCTAGAA GACGCTGAAC CCCAGAATGC AGACATCGGC AGAAAGCTAA 
     4501 AAAGATCTGA AATGCTTTAC ACACCTGAAC CAAATGGAAT GGCTTCTGAG GAAGTGACGG 
     4561 AGAAGGAAAG ACAGAAAGAG CAGGTGCATG CCGATGGGTC ATGCAGCCCT CAGGAAGAGA 
     4621 CGGCCATGAC AGAGCATCAG ATGGAAGGCC CCCCTGAGGA AGCAGAGAGT CTTCATGAGG 
     4681 AGGAAGAAAC TCTTGCTTCC TGTGAGCCAG CTCCTGAGAT TCCTAGGGAG AATCAAACCA 
     4741 CAGTGAGAAG TCTGTCCCCG TCTCCTGACT CCTCCACGGC AGCAGATCCG CCCACTCCTC 
     4801 CACAGCTCAG GGAAGGATCG CATTTCATGT GTGTGACGCG TACGCGGCCG CTCGAGCAGA 
     4861 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4921 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4981 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5041 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5101 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5161 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5221 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5281 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5341 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5401 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5461 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5521 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5581 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5641 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5701 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5761 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5821 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5881 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5941 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6001 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6061 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6121 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6181 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6241 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6301 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6361 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6421 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6481 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6541 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6601 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6661 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6721 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6781 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6841 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6901 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6961 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7021 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7081 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7141 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7201 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7261 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7321 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7381 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7441 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7501 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7561 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7621 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7681 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7741 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7801 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7861 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7921 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7981 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8041 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8101 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8161 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8221 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8281 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8341 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8401 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8461 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8521 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8581 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8641 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//