LOCUS       MR212442                9414 bp    DNA     circular  SYN 
DEFINITION  Nrxn2 (Myc-DDK-tagged) - Mouse neurexin II (Nrxn2), transcript variant 2
ACCESSION   NM_020253
VERSION     NM_020253.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5571..5662 
                     /label="Myc-DDK"  
     misc_feature    5692..6277 
                     /label="PolyA signal"  
     misc_feature    5749..5768 
                     /label="Restriction Enzyme"  
     misc_feature    6553..7225 
                     /label="ColE1"  
     misc_feature    7655..8449 
                     /label="Kan/Neo"  
     misc_feature    8484..8786 
                     /label="SV40 ori"  
     misc_feature    8872..9349 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5553..5559 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5553 
                     /label="Nrxn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTCGGG AGTCGGTGGC AACCGCCACC CCAGCTGCCG CCGCTGCTGT 
     1081 TGCTGCTGGC GCTGGCGGCA GGCGTCCGTG GCTTGGAGTT CGGCGGCGGC CCCGGGCAGT 
     1141 GGGCTCGCTA CGCGCGTTGG GCGGGAGCGG CGAGCACCGG CGAGCTCAGC TTCAGCCTGC 
     1201 GCACCAACGC CACGCGCGCG CTGCTGCTCT ACCTGGACGA CGGCGGCGAC TGCGACTTCT 
     1261 TGGAGCTGCT GCTGGTGGAC GGGCGCCTGC GGCTGCGCTT CACGCTGTCT TGCGCGGAGC 
     1321 CCGCCACGCT GCAGCTGGAC ACGCCGGTGG CCGACGACCG CTGGCACATG GTGTTGCTGA 
     1381 CCCGCGACGC GCGGCGCACG GCGCTGGCGG TGGACGGCGA AGCCCGCGCC GCCGAGGTCC 
     1441 GCTCAAAGCG GCGCGAGATG CAGGTGGCCA GCGACCTGTT CGTGGGCGGC ATCCCTCCCG 
     1501 ACGTGCGCCT ATCTGCACTC ACGCTCAGCA CCGTCAAGTA CGAGCCGCCT TTCCGCGGCC 
     1561 TTCTGGCCAA CCTGAAGCTG GGCGAGCGGC CGCCGGCGCT GCTGGGTAGC CAGGGTCTGC 
     1621 GCGGTGCGGC CGCCGACCCT CTGTGTGCGC CTGCGCGCAA TCCCTGCGCC AACGGCGGTC 
     1681 TCTGCACCGT GCTAGCCCCC GGCGAGGTGG GCTGCGACTG CAGCCACACT GGATTTGGCG 
     1741 GCAAGTTCTG CAGTGAAGAG GAACACCCCA TGGAAGGTCC GGCTCACCTG ACGTTAAACA 
     1801 GCGAAGTAGG GTCCTTACTG TTCTCCGAGG GGGGGGCCGG GAGAGGAGGA GCCGGCGATG 
     1861 TGCACCAGCC GACAAAAGGC AAGGAGGAAT TTGTGGCAAC CTTCAAGGGC AATGAGTTCT 
     1921 TCTGCTACGA CCTGTCCCAC AACCCGATCC AGAGCAGCAC TGACGAGATC ACACTGGCCT 
     1981 TCCGCACCCT GCAGCGCAAC GGGCTGATGC TGCACACGGG GAAGTCGGCT GACTACGTCA 
     2041 ACCTGTCCCT CAAGTCTGGG GCTGTCTGGC TGGTCATCAA CCTAGGCTCA GGTGCCTTCG 
     2101 AGGCCCTCGT GGAACCCGTC AATGGCAAGT TCAACGACAA CGCCTGGCAC GACGTCCGGG 
     2161 TTACCCGAAA CCTGCGCCAG CACGCAGGGA TTGGACACGC TATGGTGACC ATCTCGGTGG 
     2221 ACGGGATCCT GACCACCACA GGCTACACGC AGGAGGATTA CACCATGCTG GGCTCTGATG 
     2281 ACTTCTTCTA CATTGGGGGC AGCCCCAACA CAGCCGACCT GCCTGGCTCA CCTGTCAGCA 
     2341 ACAACTTCAT GGGCTGCCTC AAGGACGTGG TCTACAAGAA TAATGACTTC AAGCTGGAGC 
     2401 TGTCCCGACT GGCTAAGGAA GGGGACCCGA AGATGAAGCT GCAGGGGGAT TTGTCTTTCC 
     2461 GTTGTGAGGA TGTGGCTGCC TTGGACCCTG TGACCTTTGA GAGTCCTGAG GCCTTTGTCG 
     2521 CACTGCCCCG CTGGAGCGCC AAGCGCACTG GTTCCATCTC CCTGGACTTC AGAACCACTG 
     2581 AGCCCAATGG GTTGTTGCTC TTCAGCCAGG GCCGGCGGGC TGGGGCCGGG GTAGGCAGTC 
     2641 ACAGTTCTAC CCAGAGGGCC GACTACTTTG CCATGGAGCT GTTGGATGGC TACCTCTACC 
     2701 TTCTGCTGGA CATGGGCTCC GGGGGCATCA AGCTGCGGGC GTCTAGCCGC AAGGTCAATG 
     2761 ATGGTGAATG GTGCCACGTG GACTTCCAGA GGGACGGGCG CAAAGGCTCC ATCTCTGTGA 
     2821 ACAGCCGCAG CACGCCATTC TTGGCCACAG GAGAGAGCGA GGTCCTGGAC CTGGAGAGTG 
     2881 AGCTGTACCT GGGCGGTCTC CCCGAGGGGG GACGAGTGGA CCTGCCACTG CCCCCTGAGG 
     2941 TGTGGACAGC TGCTCTCCGG GCTGGCTACG TGGGCTGTGT GAGAGACCTC TTCATCGATG 
     3001 GACGGAGTCG AGATCTCCGG GGCCTGGCTG AGGCCCAGGG GGCTGTGGGC GTTGCTCCTT 
     3061 TCTGCTCCCG GGAGACCCTG AAGCAGTGTG CGTCGGCCCC TTGTCGAAAC GGGGGCATCT 
     3121 GTCGAGAGGG CTGGAACCGG TTCGTCTGTG ACTGCATCGG GACCGGCTTT CTGGGTCGGG 
     3181 TCTGCGAGAG AGAGGCCACG GTCTTAAGCT ATGACGGCTC CATGTACATG AAGATCATGC 
     3241 TGCCCACTGC GATGCACACG GAAGCAGAGG ATGTGTCCTT ACGCTTCATG TCTCAGAGGG 
     3301 CATATGGACT CATGATGGCC ACCACCTCCA GGGAGTCGGC TGACACTCTG CGTCTCGAGC 
     3361 TGGACGGGGG GCAGATGAGG CTCACCGTCA ACCTCGACTG CCTGCGCGTC GGCTGCGCAC 
     3421 CCAGTAAAGG CCCCGAGACA CTGTTTGCGG GGCACAAGCT GAATGACAAT GAATGGCACA 
     3481 CGGTGAGGGT GGTCCGGCGT GGCAAGAGCC TGCAGCTGTC CGTGGACAAC GTGACTGTGG 
     3541 AGGGACAGAT GGCAGGAGCC CACACTCGGC TGGAGTTCCA CAACATTGAG ACGGGCATTA 
     3601 TGACAGAGCG GCGGTTTATC TCTGTGGTGC CCTCCAACTT CATCGGGCAC CTGAGTGGGC 
     3661 TGGTGTTCAA TGGTCAACCC TACATGGACC AATGCAAAGA TGGAGACATC ACCTATTGTG 
     3721 AGCTTAATGC CCGCTTTGGC CTGCGTGCCA TCGTGGCTGA TCCTGTCACG TTCAAGAGTC 
     3781 GCAGCAGCTA CCTGGCGTTA GCCACGCTGC AAGCCTATGC TTCCATGCAT CTCTTCTTCC 
     3841 AGTTCAAGAC CACGGCCCCA GATGGACTTC TGCTGTTCAA CTCAGGCAAC GGCAACGACT 
     3901 TCATCGTCAT CGAGTTGGTC AAGGGGTACA TCCACTACGT GTTTGACCTG GGAAATGGCC 
     3961 CGTCCTTGAT GAAGGGAAAC TCAGACAAAC CAGTCAATGA CAACCAATGG CACAATGTGG 
     4021 TGGTGTCCAG GGACCCAGGC AACGTGCACA CACTGAAGAT CGACTCCCGC ACAGTCACGC 
     4081 AGCATTCCAA CGGTGCCCGA AATCTGGATC TCAAAGGGGA GTTGTATATC GGTGGCCTGA 
     4141 GCAAGAATAT GTTCAGCAAC CTGCCCAAGC TGGTGGCCTC TCGGGATGGC TTTCAAGGCT 
     4201 GCTTGGCTTC TGTGGACCTC AACGGACGCC TCCCAGACCT CATCGCTGAC GCCCTGCACC 
     4261 GCATCGGGCA GGTGGAGAGG GGCTGTGATG GCCCTAGCAC CACCTGCACC GAAGAGTCTT 
     4321 GTGCCAACCA GGGCGTCTGT CTGCAGCAGT GGGATGGTTT TACCTGTGAC TGTACTATGA 
     4381 CTTCCTATGG AGGCCCTGTC TGCAATGACC CTGGGACCAC ATACATCTTC GGGAAGGGGG 
     4441 GAGCGCTCAT CACCTATACA TGGCCTCCCA ATGACCGGCC CAGTACACGG ATGGACCGCC 
     4501 TGGCCGTAGG CTTCAGCACA CACCAGCGGA GCGCTGTGCT GGTGCGAGTG GACAGTGCCT 
     4561 CCGGCCTCGG GGACTACTTG CAGCTGCACA TTGACCAGGG CACTGTTGGG GTGATTTTTA 
     4621 ATGTGGGCAC GGACGACATT ACAATTGATG AGCCCAACGC CATCGTGAGC GACGGCAAAT 
     4681 ATCACGTGGT GCGCTTTACT CGAAGTGGTG GCAATGCCAC CTTGCAAGTG GACAGCTGGC 
     4741 CAGTCAACGA GAGGTACCCG GCAGGAAACT TTGATAACGA GCGCCTGGCG ATTGCTAGAC 
     4801 AGAGAATCCC CTACCGGCTT GGTCGAGTAG TAGATGAGTG GCTGCTCGAC AAAGGACGCC 
     4861 AGCTGACCAT CTTCAACAGC CAGGCTGCCA TCAAGATAGG GGGCCGGGAT CAGGGCCGCC 
     4921 CCTTCCAGGG CCAGGTGTCC GGCCTCTACT ACAATGGGCT CAAGGTACTG GCGCTGGCCG 
     4981 CCGAGAGCGA CCCCAATGTG CGGACCGAAG GCCACCTACG GCTAGTAGGG GAGGGGCCGT 
     5041 CCGTGCTGCT CAGTGCTGAG ACCACTGCCA CCACTCTGCT GGCCGACATG GCCACCACCA 
     5101 TCATGGAGAC CACCACCACC ATGGCCACCA CCACTACTCG CCGGGGCCGT TCCCCCACAA 
     5161 TGAGGGACAG CACCACCCAG AACACAGATG ACCTCCTGGT GGCCTCGGCT GAGTGTCCAA 
     5221 GTGATGATGA GGACCTAGAG GAGTGTGAGC CTAGTACTGC CAACCCCACG GGTCCGGGGG 
     5281 AGCGCGGCCC GCCAGGTGCA GTGGAGGTGA TCCGCGAATC CAGCAGCACC ACGGGCATGG 
     5341 TGGTGGGCAT CGTGGCGGCG GCGGCGCTCT GCATCCTCAT TCTCCTCTAC GCCATGTACA 
     5401 AGTACCGCAA CCGCGACGAG GGCTCCTACC AGGTGGACCA GAGCCGGAAT TACATCAGTA 
     5461 ACTCGGCCCA GAGCAATGGG GCGGTGGTGA AGGAGAAGGC CCCTGCTGCC CCCAAGACGC 
     5521 CAAGCAAGGC CAAGAAGAAC AAAGACAAAG AGTATTACGT CACGCGTACG CGGCCGCTCG 
     5581 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5641 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5701 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5761 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5821 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5881 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5941 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     6001 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     6061 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     6121 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6181 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6241 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6301 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6361 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6421 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6481 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6541 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6601 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6661 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6721 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6781 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6841 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6901 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6961 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     7021 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     7081 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     7141 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7201 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7261 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7321 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7381 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7441 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7501 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7561 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7621 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7681 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7741 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7801 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7861 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7921 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7981 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     8041 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     8101 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     8161 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8221 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8281 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8341 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8401 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8461 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8521 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8581 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8641 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8701 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8761 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8821 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8881 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8941 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     9001 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     9061 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     9121 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9181 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9241 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9301 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9361 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//