LOCUS       MR212415                7002 bp    DNA     circular  SYN 
DEFINITION  Fbxl18 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 18 (Fbxl18)
ACCESSION   NM_001033312
VERSION     NM_001033312.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3159..3250 
                     /label="Myc-DDK"  
     misc_feature    3280..3865 
                     /label="PolyA signal"  
     misc_feature    3337..3356 
                     /label="Restriction Enzyme"  
     misc_feature    4141..4813 
                     /label="ColE1"  
     misc_feature    5243..6037 
                     /label="Kan/Neo"  
     misc_feature    6072..6374 
                     /label="SV40 ori"  
     misc_feature    6460..6937 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3141..3147 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3141 
                     /label="Fbxl18"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAGTGAG GAGGAGGCAG CGGCAGAGGC AGCAGGTGAC ACCCACCTGC 
     1081 TTGGCTTCTC CGATGAGATC CTCCTACACA TCCTGAGCCA TGTCCCCAGC ACGGACCTGG 
     1141 TTCTCAGTGT GCGGCGCACC TGCAGGAAAC TGGCAGCCCT GTGCCTGGAC AAGAGCCTGG 
     1201 TGCACACCGT GCTGCTGCAG AAGGACTACC AGGCCAGTGA GGAGAAGGTG AAGCAACTGG 
     1261 TGAAGGAGAT TGGCCGGGAG ATCCAACAGC TGAACATGGC AGGCTGCTAT TGGTTGTCTG 
     1321 GCTCCACCAT CGAGCACGTG GCCCGGTGTC ACAGCCTGGT GAAGGTGAAC TTGTCAGGTT 
     1381 GCCACCTCAC CTCGCTGCGG CTGTCCAAGG TGCTCTCGGC CCTGCAGCAC CTGCGCTCAT 
     1441 TGGCCATTGA CGTGAGCCCC GGCTTCGACG CCAGCCAGCT GAGCAGTGAG TGCAAGGCCA 
     1501 CGCTAAGCCG CGTGCAGGAG CTCAAGCAGA CACTCTTCAC GCCGTCCTAC GGGGTGGTGC 
     1561 CCTGCTGTGC CAGCCTGCAG AAGCTGCTGC TCTACTTCGA GATCCTGGAC CGCACCCGGG 
     1621 AGGGGGCCGT CCTCTCGGGC CAGCTCATGG TGGGCCAGAG CAACGTGCCC CACTACCAGA 
     1681 ACCTGCGTGT CTTCTATGCA CGCCTGGCTC CTGGCTACAT CAACCAGGAG GTGGTGAGAC 
     1741 TCTACCTGGC CGTGCTCAGC GACCGCACGC CTGAGAACCT GCATGCCTTC CTCATCTCCG 
     1801 TCCCTGGCAG CTTTGCAGAG AGCGGGGCCA CCAAAAACTT GCTGGACTCC ATGGCCCGCA 
     1861 ACGTGGCCCT GGATGCACTG CAGCTGCCCA AGTCCTGGCT CAATGGCTCC ACCCTCCTCC 
     1921 AGCATATGAA ATTCAACAAT CCCTTCTACT TCAGCTTCAG CCGGTGTACG CTGTCAGGGG 
     1981 GTCACCTGAT TCAGCGGCTC ATCAATGGCG GGAAGGACCT GCGTAGCCTG GCCAGCCTCA 
     2041 ACCTCAGCGG CTGTGTGCAT TGCCTGTCTG CGGACTCGTT GCTGCGCAAG GCCGAGGACG 
     2101 ACATTGACAG CAGCATCCTG GAGACTCTGG TGGAGTCCTG CTGCAACCTG CACCACCTCA 
     2161 ATCTCTCTGC CGCCCATCAC CACAGCTCCG ACGGCCTGGG CCGCCACCTC TGCCAGCTCC 
     2221 TGGCTCGGCT CTGCCACCTG CGTTCCCTGT CCCTGCCTGT CTGCTCTGTG GCTGACTCGG 
     2281 CACCCAGGCC TGACCGTGCG CCCGCCCCGC CAGCCATGCA CGCAGTACCC CGTGGATTTG 
     2341 GCAAGAAGGT GCGCATTGGG GTGCAGACCT GTCCCAACCC CTTCGTGGGC CAGTCGGCCC 
     2401 CACAGCCTGC CTCTGTGTTC TGGTCTCTGC TGAAGAAGCT CCCATTTCTG GAGCACCTGG 
     2461 AACTGATCGG GTCCAATTTC TCCTCAGCCA TGCCCCGCAA TGAGCCTGCC ATCCGCAACT 
     2521 CCCTCCCACC CTGCAGTCGG GCACAGAACG TGGGGGACTC AGAGGTGGCC GCCATTGGCC 
     2581 AGCTGACCTT CCTGCGGCAC CTGACTCTAG CCCAACTGCC AGGCATGCTC ACGGGCTCCG 
     2641 GACTCGTGAG CATCGGTCTC CAGTGCCAGC ACCTGCAGTC TCTATCGCTG GCCAACCTGG 
     2701 GCATGATGGG GAAGGTGGTG TACATGCCTG CTCTTGCTGA CATGCTGAAG CACTGCAAGC 
     2761 GGCTGAAGGA CCTCAGGTTG GAGCAGCCCT ACTTCAACGC CAACGCTCAG TTCTTCCAGG 
     2821 CACTGGGCCA GTGCTCGTCT CTGCAGCGCC TGTGCCTGGT ATCTCGCAGC GGCACCCTGC 
     2881 AGCCAGATGC CGTGCTGGCC TTTATGGCCC GTTGCCTGCA GGTCGTCATG TGTCACATGT 
     2941 TCACCGGAGA ATCCCTTACC ACCTGCAAGA GCCTGCAGCA GTCGCTCCTC CGGAGTTTCC 
     3001 AGGCCGAGAG GCCCGCGTTG AACGTCGTCA TCTTCCCCCT GCTCCACGAG GGCCTGACAG 
     3061 ATGTCATCCG AGATGTCCCC ATGCTGCACC TGGATGAGAT CACCTTGTTC AAAAGCCGTG 
     3121 TGGCTGAGGA ACCGCCCAAC CTATGGTGGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3181 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3241 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3301 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3361 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3421 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3481 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3541 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3601 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3661 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3721 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3781 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3841 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3901 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3961 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4021 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4081 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4141 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4201 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4261 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4321 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4381 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4441 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4501 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4561 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4621 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4681 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4741 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4801 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4861 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4921 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4981 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5041 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5101 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5161 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5221 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     5281 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5341 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5401 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5461 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5521 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5581 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5641 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5701 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5761 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5821 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5881 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5941 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6001 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6061 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6121 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6181 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6241 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6301 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6361 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6421 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6481 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6541 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6601 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6661 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6721 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6781 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6841 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6901 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6961 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//