LOCUS       MR212350                7935 bp    DNA     circular  SYN 
DEFINITION  Kcnh4 (Myc-DDK-tagged) - Mouse potassium voltage-gated channel, subfamily H (eag-related), member 4 (Kcnh4)
ACCESSION   NM_001081194
VERSION     NM_001081194.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4092..4183 
                     /label="Myc-DDK"  
     misc_feature    4213..4798 
                     /label="PolyA signal"  
     misc_feature    4270..4289 
                     /label="Restriction Enzyme"  
     misc_feature    5074..5746 
                     /label="ColE1"  
     misc_feature    6176..6970 
                     /label="Kan/Neo"  
     misc_feature    7005..7307 
                     /label="SV40 ori"  
     misc_feature    7393..7870 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4074..4080 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4074 
                     /label="Kcnh4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTCATG AAGGGGTTGC TGGCCCCGCA AAACACCTTT CTGGACACCA 
     1081 TCGCCACCCG CTTTGACGGC ACGCACAGCA ACTTCCTTCT GGCCAATGCA CAGGGCCCAC 
     1141 GGGGTTTTCC CATCGTCTAC TGCTCTGACG GCTTCTGTGA GCTCACAGGC TACGGCCGCA 
     1201 CCGAGGTCAT GCAGAAAACC TGTAGCTGTC GGTTCCTCTA TGGCCCAGAG ACCAGTGAAC 
     1261 CAGCCCTGCA ACGGTTGCAA AAAGCCCTGG AGGGCCACCA AGAACACCGA ACTGAAATCT 
     1321 GCTTTTACCG AAAGGATGGC TCAGCCTTCT GGTGTCTTCT GGACATGATG CCCATCAAGA 
     1381 ATGAGATGGG GGAGGTTGTG CTTTTCCTAT TTTCCTTCAA GGACATCTCT CAGAGTGGAG 
     1441 GCCCAGGACT TGGCTCACCA GGAATCCATG GGGACAATAA TCACGAAAAC TCTCTTGGGA 
     1501 GGAGAGGAAC TAGCTCAAGA CTTAGGTCCA CGAAGAGGCA GAACCGGACT GTCCTACACC 
     1561 GGTTGACTGG CCACTTTGGT CGCCGGGGCC AGAGCAGTGT GAAGGCCAAC AGTAACCTGT 
     1621 TTGAGCCAAA ACCATCAGTG CCCGAGTACA AGGTGGCCTC TGTGGGGGGC TCCCGCTGCC 
     1681 TCCTCCTCCA CTACAGCATC CCCAAGGCTG TCTGGGATGG TCTCATCCTC CTCGCCACAT 
     1741 TCTACGTCGC GGTCACTGTC CCCTACAACG TCTGCTTCGC GGGTGATGAC GACACCCCCA 
     1801 TCACTTCCCG ACACACCCTT GTCAGTGACA TCGCTGTGGA AATGCTCTTC ATCCTGGACA 
     1861 TCATCTTGAA CTTCCGCACC ACCTACGTGT CCCAGTCTGG CCAGGTGATT TCGGCTCCTC 
     1921 GGTCCATTGG CCTCCACTAT CTGGCCACCT GGTTCTTTGT AGACCTCATT GCTGCTTTGC 
     1981 CCTTTGATCT GCTGTATGTC TTCAACATCA CTGTGACCTC ACTGGTACAT CTGCTGAAAA 
     2041 CCGTGCGGCT CCTGCGGCTG CTGCGGCTGC TGCAGAAGTT AGAGCGGTAC TCACAGTGCA 
     2101 GTGCAGTGGT GCTCACGCTG CTCATGTCCG TCTTTGCCCT CCTTGCTCAT TGGATGGCCT 
     2161 GCGTCTGGTA TGTCATCGGG CGCCGGGAGA TGGAGGCCAA TGATCCACTG CTCTGGGACA 
     2221 TTGGTTGGTT GCATGAGCTG GGCAAGCGGT TGGAGGAGCC TTATGTCAAT GGCTCGGCAG 
     2281 GTGGACCATC TCGGCGCAGT GCCTACATCG CTGCGCTGTA CTTCACGCTG AGCAGCCTCA 
     2341 CCAGTGTAGG CTTCGGCAAC GTGTGTGCCA ACACAGACGC TGAGAAGATC TTCTCCATCT 
     2401 GCACGATGCT CATAGGCGCG CTGATGCACG CAGTGGTGTT TGGGAATGTC ACAGCCATCA 
     2461 TCCAGCGCAT GTACTCCCGA CGCTCGCTCT ACCACAGCCG CATGAAGGAT CTCAAGGACT 
     2521 TCATCCGAGT GCATCGCCTG CCCCGCCCAC TCAAGCAGCG CATGCTCGAG TACTTCCAGA 
     2581 CGACGTGGGC AGTCAACAGC GGCATCGATG CCAACGAGTT ACTGCGTGAC TTCCCGGATG 
     2641 AGCTGCGTGC TGACATTGCT ATGCACCTGA ACAGGGAGAT CCTGCAGCTG CCTCTGTTTG 
     2701 GAGCGGCTAG CAGGGGCTGC CTGCGGGCCC TCTCCTTGCA CATCAAGACC TCCTTCTGTG 
     2761 CTCCTGGGGA GTACCTGTTA CGCCGTGGGG ATGCCCTCCA GGCACACTAC TACGTCTGCT 
     2821 CTGGCTCGCT TGAGGTGCTC CGGGACAACA TGGTGCTGGC CATCCTGGGG AAGGGGGACT 
     2881 TGATTGGGGC AGACATCCCT GAGTTGGGAC AGGAGCCTGG GTCAGGGGCA GGCCCCAGCT 
     2941 GTGTGCTGAA GACCAGCGCC GATGTGAAAG CTCTGACTTA CTGTGGCCTG CAGCAGCTGA 
     3001 GCAGCCGAGG GCTGGCGGAG GTCCTGCGGC TGTATCCGGA ATATGCAGCT GCCTTCCGGG 
     3061 CTGGCCTACC CCGGGACCTA ACCTTCAACC TGCGCCAAGG TTCTGAAAAC AATGGCCTCG 
     3121 GCCGCTTCTC ACGGTCTCCT CGTCTCTCCC AGGCACGCTC CGACACTCTT GGTTCCTCCT 
     3181 CAGACAAGAC TCTGCCATCC ATCACAGAAA CCGAGGGTGG CACGGAGCCT GGGGCTGGTT 
     3241 CCAAGCCCCG TCGGCCCCAC CTGCTGCCCA ACCTCAGTCC AGCACGACCT CGGGGGTCCC 
     3301 TGGTCAGCCT TTTGGGCGAG GAGCTGCCCC CATTCTCAGC CCTTGTCTCC TCTCCTTCCC 
     3361 TGTCCCCAAC TCCTTCCCCT GCCCTGGCTG GCCGGGGTCA GAGTCCCTCC CCGCACGGCC 
     3421 CCCCCAGGGG CTCTGCTGCC TGGAAGCCCC CCCAGCTCCT CATTCCCCCA CTGGGAACAT 
     3481 TTGGACCTCC GGACCTCAGT CCCCGGATCG TGGATGGCAT TGAAGACTCC AGTAACACAG 
     3541 CTGAGGCTCC TACATTCCGC TTCAGCAAGA GGCCAGAGCC CACCAGAACT CGCTCACAGG 
     3601 CTCCCCCTTC AGGCCCCAGG CTCAGCCGGG AACTGGCCAC AGAGGCAGCA GAGGAGGTGA 
     3661 AGGAAAAGGT CTGCAGGCTG AACCAAGAGA TCTCCCGTCT CAATCAGGAA GTGTCTCAGC 
     3721 TGAGCCGGGA GCTGCGGCAA GTGATGGGTC TCTTACAGGC CAGGCTGGGA CCCCCAAGTC 
     3781 ACACAGCTGG TCCAGCTTGG CTCCCAGACC TTCCTTGCCC CCACCAGAGA CTACCATGCA 
     3841 TCTCTCCTCA TATGTCTGGA CCACCACCTG GTCTCCAGAA TACTACACTC GCTGTAGTCC 
     3901 ACTGTCCAGC CAGTGTTGGG ACAGTGGAGA TAGGGGCCAC CCCCTCAGAG CTGAGACCTT 
     3961 CCACGATACC ACCCTATCCC TCGGAACCTG ATCCTCTGGG ACCCTCTCCA GCGCCAGAGG 
     4021 CTCCTCCCCT GACCCCAAGC CTCCTGAAGC ACAGCTTCCA GTCTGGGTCA GACACATTCC 
     4081 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4141 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4201 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4261 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4321 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4381 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4441 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4501 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4561 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4621 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4681 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4741 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4801 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4861 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4921 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4981 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5041 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5101 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5161 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5221 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5281 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5341 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5401 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5461 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5521 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5581 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5641 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5701 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5761 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5821 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5881 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5941 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6001 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6061 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6121 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6181 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6241 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6301 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6361 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6421 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6481 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6541 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6601 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6661 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6721 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6781 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6841 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6901 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6961 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7021 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7081 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7141 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7201 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7261 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7321 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7381 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7441 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7501 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7561 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7621 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7681 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7741 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7801 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7861 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7921 TTTAACGCGA ATTTT
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