LOCUS       MR212152                8535 bp    DNA     circular  SYN 
DEFINITION  Jag1 (Myc-DDK-tagged) - Mouse jagged 1 (Jag1)
ACCESSION   NM_013822
VERSION     NM_013822.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4692..4783 
                     /label="Myc-DDK"  
     misc_feature    4813..5398 
                     /label="PolyA signal"  
     misc_feature    4870..4889 
                     /label="Restriction Enzyme"  
     misc_feature    5674..6346 
                     /label="ColE1"  
     misc_feature    6776..7570 
                     /label="Kan/Neo"  
     misc_feature    7605..7907 
                     /label="SV40 ori"  
     misc_feature    7993..8470 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4674..4680 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4674 
                     /label="Jag1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGTCCCCA CGGACGCGCG GCCGGCCCGG GCGCCCCCTG AGTCTTCTGC 
     1081 TCGCCCTGCT CTGTGCCCTG CGAGCCAAGG TGTGCGGGGC CTCGGGTCAG TTTGAGCTGG 
     1141 AGATCCTGTC CATGCAGAAC GTGAATGGAG AGCTACAGAA TGGGAACTGT TGTGGTGGAG 
     1201 TCCGGAACCC TGGCGACCGC AAGTGCACCC GCGACGAGTG TGATACGTAC TTCAAAGTGT 
     1261 GCCTCAAGGA GTATCAGTCC CGCGTCACTG CCGGGGGACC CTGCAGCTTC GGCTCAGGGT 
     1321 CTACGCCTGT CATCGGGGGT AACACCTTCA ATCTCAAGGC CAGCCGTGGC AACGACCGTA 
     1381 ATCGCATCGT ACTGCCTTTC AGTTTCGCCT GGCCGAGGTC CTACACTTTG CTGGTGGAGG 
     1441 CCTGGGATTC CAGTAATGAC ACTATTCAAC CTGATAGCAT AATTGAAAAG GCTTCTCACT 
     1501 CAGGCATGAT AAACCCTAGC CGGCAATGGC AGACACTGAA ACAAAACACA GGGATTGCCC 
     1561 ACTTCGAGTA TCAGATCCGA GTGACCTGTG ATGACCACTA CTATGGCTTT GGCTGCAATA 
     1621 AGTTCTGTCG TCCCAGAGAT GACTTCTTTG GACATTATGC CTGTGACCAG AACGGCAACA 
     1681 AAACTTGCAT GGAAGGCTGG ATGGGTCCTG ATTGCAACAA AGCTATCTGC CGACAGGGCT 
     1741 GCAGTCCCAA GCATGGGTCT TGTAAACTTC CAGGTGACTG CAGGTGCCAG TACGGTTGGC 
     1801 AGGGCCTGTA CTGCGACAAG TGCATCCCGC ACCCAGGATG TGTCCACGGC ACCTGCAATG 
     1861 AACCCTGGCA GTGCCTCTGT GAGACCAACT GGGGTGGACA GCTCTGTGAC AAAGATCTGA 
     1921 ATTACTGTGG GACTCATCAG CCCTGTCTCA ACCGGGGAAC ATGTAGCAAC ACTGGGCCTG 
     1981 ACAAATACCA GTGCTCCTGC CCAGAGGGCT ACTCGGGCCC CAACTGTGAA ATTGCTGAGC 
     2041 ATGCTTGTCT CTCTGACCCC TGCCATAACC GAGGCAGCTG CAAGGAGACC TCCTCAGGCT 
     2101 TTGAGTGTGA GTGTTCTCCA GGCTGGACTG GCCCCACGTG TTCCACAAAC ATCGATGACT 
     2161 GTTCTCCAAA TAACTGTTCC CATGGGGGCA CCTGCCAGGA TCTGGTGAAT GGATTCAAGT 
     2221 GTGTGTGCCC GCCCCAGTGG ACTGGCAAGA CTTGTCAGTT AGATGCAAAT GAGTGCGAGG 
     2281 CCAAACCTTG TGTAAATGCC AGATCCTGTA AGAATCTGAT TGCCAGCTAC TACTGTGATT 
     2341 GCCTTCCTGG CTGGATGGGT CAGAACTGTG ACATAAATAT CAATGACTGC CTTGGCCAGT 
     2401 GTCAGAATGA CGCCTCCTGT CGGGATTTGG TTAATGGTTA TCGCTGTATC TGTCCACCTG 
     2461 GCTATGCAGG CGATCACTGT GAGAGAGACA TCGATGAGTG TGCTAGCAAC CCCTGCTTGA 
     2521 ATGGGGGTCA CTGTCAGAAT GAAATCAACA GATTCCAGTG TCTCTGTCCC ACTGGTTTCT 
     2581 CTGGAAACCT CTGTCAGCTG GACATCGATT ACTGCGAGCC CAACCCTTGC CAGAATGGCG 
     2641 CCCAGTGCTA CAATCGTGCC AGTGACTATT TCTGCAAGTG CCCCGAGGAC TATGAGGGCA 
     2701 AGAACTGCTC ACACCTGAAA GACCACTGCC GTACCACCAC CTGCGAAGTG ATTGACAGCT 
     2761 GCACTGTGGC CATGGCCTCC AACGACACGC CTGAAGGGGT GCGGTATATC TCTTCTAACG 
     2821 TCTGTGGTCC CCATGGGAAG TGCAAGAGCC AGTCGGGAGG CAAATTCACC TGTGACTGTA 
     2881 ACAAAGGCTT CACCGGCACC TACTGCCATG AAAATATCAA CGACTGCGAG AGCAACCCCT 
     2941 GTAAAAACGG TGGCACCTGC ATCGATGGCG TTAACTCCTA CAAGTGTATC TGTAGTGACG 
     3001 GCTGGGAGGG AGCGCACTGT GAGAACAACA TAAATGACTG TAGCCAGAAC CCTTGTCACT 
     3061 ACGGGGGTAC ATGTCGAGAC CTGGTCAATG ACTTTTACTG TGACTGCAAA AATGGCTGGA 
     3121 AAGGAAAGAC TTGCCATTCC CGTGACAGCC AGTGTGACGA AGCCACGTGT AATAATGGTG 
     3181 GTACCTGCTA TGATGAAGTG GACACGTTTA AGTGCATGTG TCCCGGTGGC TGGGAAGGAA 
     3241 CAACCTGTAA TATAGCTAGA AACAGTAGCT GCCTGCCGAA CCCCTGTCAT AATGGAGGTA 
     3301 CCTGCGTGGT CAATGGAGAC TCCTTCACCT GTGTCTGCAA AGAAGGCTGG GAGGGGCCTA 
     3361 TTTGTACTCA AAATACCAAC GACTGCAGTC CCCATCCTTG TTACAATAGC GGGACCTGTG 
     3421 TGGACGGAGA CAACTGGTAT CGGTGCGAAT GTGCCCCGGG TTTTGCTGGG CCAGACTGCA 
     3481 GGATAAACAT CAATGAGTGC CAGTCTTCCC CTTGTGCCTT TGGGGCCACC TGTGTGGATG 
     3541 AGATCAATGG CTACCAGTGT ATCTGCCCTC CAGGACATAG TGGTGCCAAG TGCCATGAAG 
     3601 TTTCAGGGCG ATCTTGCATC ACCATGGGGA GAGTGATACT TGATGGGGCC AAGTGGGATG 
     3661 ATGACTGTAA CACCTGCCAG TGCCTGAATG GACGGGTGGC CTGCTCCAAG GTCTGGTGTG 
     3721 GCCCGAGACC TTGCAGGCTC CACAAAAGCC ACAATGAGTG CCCCAGTGGG CAGAGCTGCA 
     3781 TCCCGGTCCT GGATGACCAG TGTTTCGTGC GCCCCTGCAC TGGTGTTGGC GAGTGTCGGT 
     3841 CCTCCAGCCT CCAGCCAGTG AAGACCAAGT GCACATCTGA CTCCTATTAC CAGGATAACT 
     3901 GTGCAAACAT CACTTTCACC TTTAACAAAG AGATGATGTC TCCAGGTCTT ACCACCGAAC 
     3961 ACATTTGCAG CGAATTGAGG AATTTGAATA TCCTGAAGAA TGTTTCTGCT GAATATTCGA 
     4021 TCTACATAGC CTGTGAGCCT TCCCTGTCAG CAAACAATGA AATACACGTG GCCATCTCTG 
     4081 CAGAAGACAT CCGGGATGAT GGGAACCCTG TCAAGGAAAT TACCGATAAA ATAATAGATC 
     4141 TCGTTAGTAA ACGGGATGGA AACAGCTCAC TTATTGCTGC GGTTGCAGAA GTCAGAGTTC 
     4201 AGAGGCGTCC TCTGAAAAAC AGAACAGATT TCCTGGTTCC TCTGCTGAGC TCTGTCTTAA 
     4261 CAGTGGCTTG GGTCTGTTGC TTGGTGACAG CCTTCTACTG GTGTGTAAGG AAGCGGCGGA 
     4321 AGCCCAGCAG CCACACTCAC TCCGCCCCCG AGGACAACAC CACCAACAAT GTGCGGGAGC 
     4381 AGCTGAACCA AATCAAAAAC CCCATCGAGA AACACGGAGC CAACACGGTC CCCATTAAGG 
     4441 ATTACGAGAA CAAAAACTCG AAAATGTCAA AAATCAGGAC ACACAACTCG GAAGTGGAGG 
     4501 AGGATGACAT GGATAAACAC CAGCAGAAAG TCCGCTTTGC CAAACAGCCA GTGTATACGC 
     4561 TGGTAGACAG AGAGGAGAAG GCCCCCAGCG GCACGCCGAC AAAACACCCG AACTGGACAA 
     4621 ATAAACAGGA CAACAGAGAC TTGGAAAGTG CCCAGAGCTT GAACCGGATG GAATACATCG 
     4681 TAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4741 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4801 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4861 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4921 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4981 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5041 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5101 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5161 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5221 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5281 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5341 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5401 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5461 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5521 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5581 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5641 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5701 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5761 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5821 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5881 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5941 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6001 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6061 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6121 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6181 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6241 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6301 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6361 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6421 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6481 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6541 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6601 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6661 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6721 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6781 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6841 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6901 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6961 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7021 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7081 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7141 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7201 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7261 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7321 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7381 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7441 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7501 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7561 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7621 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7681 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7741 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7801 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7861 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7921 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7981 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8041 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8101 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8161 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8221 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8281 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8341 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8401 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8461 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8521 TTTAACGCGA ATTTT
//