LOCUS       MR212025                9489 bp    DNA     circular  SYN 
DEFINITION  Eif2ak4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 4 (Eif2ak4), transcript variant 2
ACCESSION   NM_001177806
VERSION     NM_001177806.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5646..5737 
                     /label="Myc-DDK"  
     misc_feature    5767..6352 
                     /label="PolyA signal"  
     misc_feature    5824..5843 
                     /label="Restriction Enzyme"  
     misc_feature    6628..7300 
                     /label="ColE1"  
     misc_feature    7730..8524 
                     /label="Kan/Neo"  
     misc_feature    8559..8861 
                     /label="SV40 ori"  
     misc_feature    8947..9424 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5628..5634 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5628 
                     /label="Eif2ak4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCACCTAC ATACCCAGAT GTGTGATGAT ATTTGAACTG GCACACCATG 
     1081 TGCAGTCGTT TCTCAGCGAG CATAACAAGC CCCCACCAAA GTCTTTCCAT GAAGAAATGC 
     1141 TGGAAAGGCA GGCTCAGGAG AAGCAGCAGA GGTTGCTGGA GGCCAGGCGG AAGGAGGAGC 
     1201 AGGAGCAACG TGAAATCCTC CACGAGATTC AGAGAAGGAA AGAGGAGATC AAGGAAGAGA 
     1261 AGAAAAGGAA GGAAATGGCT AAGCAGGAAC GTTTGGAAAT CACTAGCTTG ACAAACCAGG 
     1321 ACTATGCCTC TAAGAGAGAC CCAGCAGGGC ACAGGGCAGC TGCCATCCTC CATGGAGGCT 
     1381 CTCCTGACTT TGTAGGAAAT GGTAAAGCCC GGACATACTC CTCAGGAAGG TCCAGGCGAG 
     1441 AACGTCAGTA TTCTGTGTGT AGTGGTGAAC CCTCTCCTGG CTCGTGTGAC ATCCTCCATT 
     1501 TCTCTGTGGG TAGCCCTGAT CAGCTCATGG TGCACAAAGG GAGATGTGTT GGCAGTGATG 
     1561 AGCAGCTGGG AAAGGTGGTA TACAATGCTT TGGAAACAGC CACGGGCAGC TTTGTCTTGC 
     1621 TGCATGAGTG GGTCCTTCAA TGGCAGAAGA TGGGCCCTTG CCTTACCAGT CAAGAGAAAG 
     1681 AGAAGATTGA CAAGTGCAAA AGGCAGATTC AAGGAGCAGA AACAGAATTC AGCTCCCTAG 
     1741 TGAAACTGAG CCATCCAAAT ATCGTCCGCT ACTTTGCGAT GAACTCCAGA GAGGAGGAGG 
     1801 ACTCCATTGT GATCGACATT CTGGCAGAGC ACGTCAGTGG CATCTCTCTG GCTACACACC 
     1861 TGAGCCACTC GGGCCCAGTC CCCGCCCACC AGCTGCGCAA GTACACAGCC CAGCTCCTGG 
     1921 CAGGCCTGGA TTACCTACAC AGCAACTCCG TGGTGCACAA GGTTCTGAGC GCGTCCAGTG 
     1981 TCTTGGTAGA TGCCGAGGGC ACTGTCAAGA TAACAGACTA CAGCATCTCT AAGCGTCTGG 
     2041 CAGACATTTG CAAGGAGGAT GTATTTGAGC AAGCTCGAGT TCGTTTTAGT GACAGTGCCC 
     2101 TGCCTTATAA AACAGGAAAG AAAGGGGATG TGTGGCGGCT CGGCCTCCTG CTGTTGTCTC 
     2161 TCAGCCAAGG ACAGGAGTGT GGGGAGTATC CGGTGACCAT CCCCAGTGAC CTGCCAGCTG 
     2221 ACTTCCAAGA CTTCCTGAAG AAGTGTGTCT GCCTGGACGA CAAGGAAAGA TGGAGCCCTC 
     2281 AGCAGCTGCT GAAACACAGC TTTATAAACC CTCAACCAAA GCTGCCTTTG GTGGAGCAGA 
     2341 GTCCTGAAGA CTCTGGGGGA CAAGACTACA TTGAGACCGT CATTCCCAGC AACCAGCTGC 
     2401 CCAGCGCTGC GTTCTTCAGT GAGACACAGA AACAGTTCTC CCGGTACTTC ATTGAGTTTG 
     2461 AAGAACTACA ACTCCTAGGG AAAGGAGCTT TTGGAGCTGT CATCAAGGTG CAAAACAAGC 
     2521 TTGATGGCTG CTGCTATGCT GTGAAGCGCA TCCCTATCAA CCCTGCAAGC AGACACTTCC 
     2581 GCCGGATCAA GGGCGAGGTG ACACTGCTAT CGCGCCTGCA CCATGAGAAC ATTGTGCGCT 
     2641 ACTACAACGC CTGGATCGAG CGGCATGAGC GCCCAGCGGT GCCAGGGACA CCGCCCCCAG 
     2701 ACTGCACACC CCAGGCCCAG GACAGCCCAG CCACCTGCGG GAAAACATCA GGCGACACTG 
     2761 AAGAACTGGG CAGCGTGGAG GCTGCAGCAC CGCCACCCAT CCTCAGCAGC TCGGTGGAGT 
     2821 GGAGCACATC TGCAGAGCGT TCTACCAGCA CCCGCTTCCC AGTCACGGGC CAGGATTCCA 
     2881 GCAGCGATGA GGAGGACGAG GACGAGCGCG ATGGCGTCTT CTCCCAGTCC TTTCTACCTG 
     2941 CTTCAGATTC TGACAGTGAC ATCATCTTTG ACAATGAAGA TGAAAACAGT AAAAGTCAAA 
     3001 ATCAGGATGA AGACTGCAAT CAAAAGGACG GTAGCCATGA GATTGAGCCT TCAGTGACAG 
     3061 CTGAGGCTGT GCACTACCTT TATATCCAGA TGGAGTACTG CGAGAAGAGC ACGCTGCGGG 
     3121 ACACCATTGA CCAGGGGCTG TTCCGAGACA CCAGCCGGCT CTGGAGGCTT TTCCGAGAGA 
     3181 TTCTGGATGG ATTAGCTTAT ATCCATGAGA AAGGAATGAT TCATCGCGAC TTGAAGCCTG 
     3241 TCAATATTTT TTTGGATTCT GATGACCATG TGAAAATAGG TGACTTTGGC CTGGCAACAG 
     3301 ACCATCTGGC CTTCACTGCT GAAGGTAAAC AGGACGACCA GGCAGGTGAC GGAGTGATTA 
     3361 AGTCAGACCC TTCAGGCCAT TTGACTGGCA TGGTTGGTAC TGCTCTGTAT GTAAGCCCTG 
     3421 AGGTCCAAGG AAGCACCAAG TCTGCATACA ACCAGAAAGT GGATCTCTTC AGCCTGGGAA 
     3481 TTATCTTCTT TGAGATGTCT TATCACCCGA TGGTGACTGC CTCAGAAAGA ATTTTCGTTC 
     3541 TCAACCAACT TCGAGATCCC ACATCGCCCA AGTTTCCAGA CGACTTCGAT GATGGAGAAC 
     3601 ATACAAAGCA GAAATCTGTC ATCTCCTGGC TGTTGAACCA TGATCCAGCT AAACGGCCCA 
     3661 CGGCCATGGA ACTGCTCAAG AGTGAGCTGC TCCCCCCGCC GCAGATGGAG GAGTCCGAGC 
     3721 TGCATGAAGT GCTGCACCAC ACGCTGGCCA ACATAGATGG GAAGGCCTAC CGCACCATGA 
     3781 TGAGTCAGAT CTTCTGCCAG CACATCTCCC CCGCTATAGA CTACACCTAC GACAGTGACA 
     3841 TCCTGAAGGG CAACTTCTTG ATTCGTACAG CCAAGATCCA GCAGCTTGTA TGTGAAACCA 
     3901 TCGTCCGCGT CTTTAAAAGG CATGGCGCTG TCCAGCTGTG CACCCCACTG CTGCTTCCCC 
     3961 GAAACAGGCA AATATATGAG CACAACGAAG CGGCTTTGTT CATGGATCAC AGCGGCATGC 
     4021 TGGTGATGCT TCCGTTCGAC CTGCGGGTCC CTTTTGCAAG ATATGTGGCA AGAAATAACA 
     4081 TACTGAATTT AAAACGATAC TGCATAGAGC GGGTGTTCAG ACCTCGGAAG TTAGACCGAT 
     4141 TTCATCCCAA AGAACTCCTG GAATGTGCAT TTGATATCGT CACTTCTACC ACCAACAGCT 
     4201 CTCTGCCCAC TGCCGAAACC ATCTACACCA TCTATGAAAT AATCCAGGAG TTTCCTGCAC 
     4261 TTCAGGAAAG GAATTACAGC ATTTACTTGA ACCATACGAT GCTTCTGAAG GCAATACTCC 
     4321 TACACTGTGG GATCCCAGAG GACAAGCTCA GCCAAGTCTA CGTCATTCTG TATGATGCTG 
     4381 TGACAGAGAA GCTGACTAGG AGAGAAGTGG AAGCTAAATT CTGTAACCTG TCCTTGTCTT 
     4441 CTAATAGCCT GTGTAGACTC TACAAGTTCA TTGAGCAGAA GGGGGATTTG CAAGACTTAA 
     4501 CGCCAACCAT CAACTCACTA ATAAAACAGA AAACAGGCGT TGCTCAGTTG GTGAAGTATA 
     4561 GCTTAAAAGA CCTCGAGGAC GTCGTCGGGC TGCTGAAGAA ACTTGGCGTG AAGTTACAGG 
     4621 TCTCCATCAA CCTGGGCTTG GTCTACAAGG TGCAGCAGCA CACTGGCATC ATCTTCCAGT 
     4681 TCCTGGCATT CAGCAAACGC AGGCAAAGGG TTGTGCCTGA GATCCTTGCC GCCGGTGGCA 
     4741 GATACGACCT GCTGATTCCC AAGTTCAGAG GCCCACAGAC TGTGGGGCCA GTCCCCACTG 
     4801 CTGTCGGTGT CAGCATAGCC ATAGACAAGA TATTTGCTGC TGTCCTCAAC ATGGAAGAGC 
     4861 CTGTTACAGT GAGCTCCTGT GACCTCCTGG TTGTCAGTGT TGGCCAGATG TCCATGTCCA 
     4921 GGGCCATCAA CCTAACCCAG AAACTGTGGA CAGCGGGCAT CACTGCAGAA ATCATGTATG 
     4981 ACTGGTCCCA GTCCCAGGAA GAGTTACAAG AGTACTGCAG ACACCATGAA ATCACCTATG 
     5041 TGGCTCTGGT CTCCGATAAA GAAGGAAGCC ATGTCAAGGT CAAGTCCTTT GAGAAGGAGA 
     5101 GGCAAACAGA AAAGCGTGTA TTGGAATCGG ATCTTGTGGA TCACGTTATG CAGAAACTAA 
     5161 GAACCAAAGT TGGTGACGAA AGAAATTTCA GAGACGCTTC TGATAATCTT GCCGTACAAA 
     5221 CTCTGAAGGG GTCGTTTTCT AATGCTTCAG GTTTGTTTGA AATCCATGGA ACTACAGTAG 
     5281 TCCCCAACGT GATTGTTCTA GCACCAGAAA AGCTGTCAGC CAGCACCAGG AGGCGACATG 
     5341 AGATTCAGGT GCAAACCCGA CTTCAGACTA CACTTGCCAA CTTACATCAG AAAAGCAGTG 
     5401 AAATTGAAAT TTTGGCTGTG GACCTACCCA AGGAAACAAT CTTACAGTTT CTATCATTAG 
     5461 AGTGGGATGC TGATGAACAG GCATTTAACA CAACTGTGAA GCAGCTGCTG TCACGCCTGC 
     5521 CAAAGCAAAG ATACCTCAAA CTCGTCTGCG ATGAAATTTA TAACATCAAA GTTGAAAAGA 
     5581 AGGTGTCAGT GCTGTTCCTG TACAGCTACA GAGATGACTA CTACAGAATC CTATTTACGC 
     5641 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5701 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5761 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5821 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5881 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5941 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     6001 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     6061 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     6121 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     6181 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     6241 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6301 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6361 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6421 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6481 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6541 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6601 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6661 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6721 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6781 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6841 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6901 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6961 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     7021 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     7081 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     7141 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7201 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     7261 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7321 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7381 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7441 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7501 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7561 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7621 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7681 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7741 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7801 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7861 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7921 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7981 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     8041 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     8101 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     8161 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     8221 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     8281 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8341 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8401 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8461 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8521 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8581 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8641 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8701 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8761 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8821 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8881 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8941 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     9001 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     9061 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     9121 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     9181 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     9241 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9301 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9361 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9421 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9481 GCGAATTTT
//