LOCUS       MR212021                9471 bp    DNA     circular  SYN 
DEFINITION  Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 1
ACCESSION   NM_001025426
VERSION     NM_001025426.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5628..5719 
                     /label="Myc-DDK"  
     misc_feature    5749..6334 
                     /label="PolyA signal"  
     misc_feature    5806..5825 
                     /label="Restriction Enzyme"  
     misc_feature    6610..7282 
                     /label="ColE1"  
     misc_feature    7712..8506 
                     /label="Kan/Neo"  
     misc_feature    8541..8843 
                     /label="SV40 ori"  
     misc_feature    8929..9406 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5610..5616 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5610 
                     /label="Depdc5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGAACCACA AAGGTCTACA AACTTGTCAT CCACAAGAAG GGCTTTGGGG 
     1081 GCAGTGATGA TGAGCTAGTC GTGAACCCTA AAGTATTTCC TCACATCAAA CTTGGAGACA 
     1141 TTGTGGAGAT TGCTCACCCC AATGATGAGT ACAGTCCTTT GCTTTTGCAA GTCAAGTCGC 
     1201 TTAAGGAAGA TTTACAGAAA GAAACTATCA GTGTGGACCA GACTGTGACT CAAGTATTCC 
     1261 GGCTAAGACC TTATCAAGAT GTCTATGTGA ATGTTGTAGA CCCCAAGGAT GTGACTCTTG 
     1321 ACCTGGTGGA ATTGACTTTT AAGGATCAGT ATATTGGCCG TGGGGATATG TGGCGACTAA 
     1381 AGAAAAGTTT GGTGAGCACC TGTGCCTATA TCACTCAAAA AGTGGAATTT GCTGGCATCA 
     1441 GGGCACAGGC TGGTGAGCTG TGGGTCAAGA ATGAGAAGGT CATGTGTGGT TACATTAGTG 
     1501 AAGAGACCAG GGTGGTGTTC CGTTCTACGT CGGCTATGGT TTACATATTT ATTCAGATGA 
     1561 GCTGTGAAAT GTGGGATTTT GATATTTATG GGGATCTGTA CTTTGAGAAA GCTGTGAACG 
     1621 GTTTCCTCGC CGACCTGTTT ACTAAATGGA AGGAGAAGAA CTGTAGTCAT GAAGTGACTG 
     1681 TGGTCCTGTT TTCCAGAACT TTCTATGATG CAAAATCTAT TGATGAATTT CCTGAAATAA 
     1741 ACCGAGCTTC AATTCAAGAG GATCACAAAG GGAGATTCTA TGAGGACTTT TACAAAGTGG 
     1801 TGGTGCAGAA TGAGAGAAGG GAAGAGTGGA CTTCACTCCT CGTGACCATT AAAAAACTCT 
     1861 TCATCCAGTA TCCAGTGTTG GTGCGACTGG AACAGGCAGG GGGCTTTCCT CAAGGAGACA 
     1921 ATTCTACCTC AGCACAAGGA AACTACCTAG AGGCCATCAA CTTGTCATTC AATGTGTTTG 
     1981 ACAAGCACTA CATCAACCGA AACTTTGACC GAACTGGGCA GATGTCTGTG GTGATCACGC 
     2041 CGGGGGTGGG TGTCTTTGAA GTGGACCGCC TACTCATGAT CTTGACCAAG CAGCGGATGA 
     2101 TTGATAATGG AATTGGTGTG GACTTAGTGT GCATGGGAGA GCAGCCACTA CATGCTGTGC 
     2161 CATTATTCAA GCTACATAAC CGGAGTGTTC CGAGGGATTC TCGACTGGGT GATGATTATA 
     2221 ATATTCCTCA CTGGATAAAC CATAGTTTCT ACACATCCAA AAGCCAGCTC TTTTGTAACA 
     2281 GTTTCACTCC ACGGATAAAA CTAGCAGGAA AGAAGTCTGC CTCTGAGAAA ACCAAAAATG 
     2341 GTCGTGATAC ATCTCTCGGG ACTCCAAAGG AATCTGAAAA CACCCTTCCC ATCCAAGTAG 
     2401 ATTATGATGC CTACGATGCT CAAGTGTTCA GGCTGCCTGG CCCATCCCGG GCTCAGCGCC 
     2461 TCGCCACCTG CAGGTCTGTG AGAGAACAGG AGAATCACAG TCGCAAAAGT GCAAGCTCCT 
     2521 GTGATGTCTC ATCCAGCCCT TCCCTGCCAA GCCGTGCACT GCCCACTGAG GAAGTGAGGA 
     2581 GCCAGGCTTC TGATGACAGC TCCCTGGGCA AGAGCACCAA CATCCTGATG ATCCCTAACC 
     2641 CCCACCTGCA CCAGTATGAA GTCAGCAGCT CACTGGGCTA CACCAGCACC CGAGATGTCC 
     2701 TGGAGAACAT GATAGAACCC CCACAGAGGG ACTCCAGTGC ACCAGGAAGG TTCCATGTGG 
     2761 GTAGTGCAGA GTCCATGCTC CATGTCCGAC CTGGAGGATA CACGCCTCAG AGAGCGCTGA 
     2821 TTAACCCCTT CGCCCCCTCG AGGATGCCCA TGAAGCTCAC CTCCAACAGA AGGCGCTGGA 
     2881 TGCACACTTT TCCTGTAGGC CCATCTGGAG AGGCCATCCA GATCCATCAT CAGACCCGGC 
     2941 AAAACATGGC AGAGCTGCAG GGCAGCAGGC AGAGGGACCC TACCCACTCC TCTGCAGAGC 
     3001 TGTTGGAGTT GGCATACCAT GAAGCTGCCG GGAGGCACAG CACTTCCCGC CAGCCTGGTG 
     3061 ACAGCATGTC CTTGAACTTC AGTGGAACGG AAGAGCTTTC TGTCAGCCTG CTTAGCAACA 
     3121 GCAGTACAGG TGTGAATCCT AGGACCCAGA ACAAGGACTC CCTAGAGGAC AGTGTTTCTA 
     3181 CCTCTCCAGA CCCAATTCTG ACACTGTCTG CTCCCCCTGT AGTGCCAGGC TTCTGTTGCA 
     3241 CAGTTGGAGT GGATTGGAAG TCTCTTACTA CTCCTGCATG CCTTCCCCTC ACCACTGACT 
     3301 ACTTCCCGGA CCGTCAGGGC CTGCAGAATG ACTACACAGA GGGCTGCTAT GATCTCCTCC 
     3361 CAGAAGCAGA CATGGACAGG AGGGATGAGG AGGGTGTTCA AATGACAGCC CAGCAAGTGT 
     3421 TTGAAGAGTT CATCTGCCAG CGTCTCATGC AGGGTTACCA AATCATAGTA CAGCCAAAGA 
     3481 CCCAGAAACC CAACACCACA GTCCCACCCC CATTGAGCAG TAGCCCACTC TATAGCAGAG 
     3541 GCCTTGTGTC CCGAAATCGC CCTGAGGAGG AAGGCCAGTA TTGGTTAAGT ATGGGCAGAA 
     3601 CTTTCCATAA GGTGACACTC AAGGACAAGA TGATCACAGT AACTCGTTAC CTTCCCAAGT 
     3661 ACCCTTATGA ATCTGCCCAG ATCCATTACA CCTACAGCCT CTGCCCTTCC CACTCTGACT 
     3721 CGGAGTTTGT CTCCTGTTGG GTGGATTTCT GCCACGAACG GCTCGAGGAA TACAAGTGGA 
     3781 ATTACTTAGA TCAGTATATT TGTTCTGCTG GCTCTGAAGA CTTCAGCTTA ATCGAGTCTC 
     3841 TGAAGTTCTG GAGAACCCGA TTTTTACTAC TGCCAGCCTG TGTTACTGCC ACCAAGCGCA 
     3901 TCACAGAAGG GGAGGTGCAC TGTGACATCT ATGGGGACAA ACCCCGTGCA GATGAAGATG 
     3961 AGTGGCAGCT TCTAGATGGC TTTATTCGCT TTGTAGAGGG CTTAAATCGC ATCCGCAGGC 
     4021 GCCACCGCTC AGACCGCATG ATTCGGAAGG GGACTGCCAT GAAAGGCTTG CAGATGACCG 
     4081 GGCCCATCTC TGCACACTCT CTCGAGGCAG CAGGCCCTCC AGTCGGCAAG AAAGGAACCT 
     4141 CAGCTCTGTC TGCCCTGCTG GAGATGGAAG CTAGTCAGAA GAGCCTAGGA GAACAGCAGA 
     4201 CGACTGTGCA CGGAAAAAGC TCCACTCAGC CAGCTGAGAA CAGCAGTGTT GCCATGACTC 
     4261 CCACCTATGT GGACAGCCCA CGCAAGGACG GGGCCTTCTT TATGGAGTTT GTCCGCAGCC 
     4321 CACGCACAGC ATCATCTGCC TTCTACCCTC AGGCATCTGT GGACCAAACA GCTCCTCTGG 
     4381 TGTTAGACAG CACCAGTTTA GGGGTCAGCA CAGGCCAGCC CATGGATAGA GGCAACAACC 
     4441 AGACCTTTGG GAACTCCCAG AACATAGAAC AGGCCTTTCC CTCTGCAAAC TCTGGTGACT 
     4501 ACAGCTCTCA GCAGCATGTA GCAAGCTCTC TAACATCGTC TTCTACCCTG GTGGAGATTC 
     4561 TGGAAGCCAT GAAGCATCCC TCGACAGGAG TCCAGCTGCT GTCTGAACAG AAGGGCCTTT 
     4621 CACCGTGCTG CTTCATCAGT GCTGAGGTCG TACACTGGTT GATGAACAAC GTGGAGGGGG 
     4681 TCCAGACGCA GGCGATGGGC ATAGACATCA TGCAGAAAAT GCTGGAAGAG CAGCTCATAA 
     4741 CGCATGCATC TGGTGAAGCA TGGCGGACCT TCATCTATGG CTTCTATTTC TACAAGATAG 
     4801 TAATGGACAA AGAACCGGAA AGAGTGGCTA TGCAGCAGCC CTCTGCCCCC TGGTACACAG 
     4861 CAGGAGCGGA TGACTTTGCC AGCTTCCAAC GCAAGTGGTT TGAGGTGGCC TTTGTGGCAG 
     4921 AAGAGCTTGT GCACTCTGAG ATTCCTGCCT TCCTCCTGCC CTGGCTACCT AGCCGGCCAG 
     4981 CCTCTTATGC AAGCAGACAC AGCTCCTTTA GCCGAAGTTT TGGAGGACGG AGCCAGGCAG 
     5041 CTGCGCTGTT AGCTGCTACT GTCCCAGAGC AGAGGACTGT GACCCTGGAT GTTGATGTGA 
     5101 ACAACCGAAC GGACCGGCTG GAATGGTGCA GCTGTTACTA CCATGGAAAC TTCTCTCTCA 
     5161 ATGCCGCCTT TGAGATCAAG CTACACTGGA TGGCAGTGAC TGCCACAGTG CTGTTTGAGA 
     5221 TGGTCCAAGG TTGGCATCGA AAAGCTACCT CCTGTGGCTT CTTGCTGGTC CCAGTTTTGG 
     5281 AGGGTCCTTT TGCTCTGCCC AGTTACCTGT ATGGTGATCC CCTGAGGGCC CAGCTCTTCA 
     5341 TCCCGCTCAA CCTCAGCTGC TTGCTCAAGG AGGGCAGCGA GCACCTGTTC GATAGTTTGG 
     5401 ATTTGTGCAA GATAAATATT CTGTCTCCGC TTTTAACTTC CCTGCTGAGA ATAAGCCACA 
     5461 GTATATCCAT GTCACAGGAA CGGTGTTCCT TCAGCTGCCG TACTCCAAAC GCAAGTTTTC 
     5521 AGGGCAGCAG CGGCGGCGAC GGAACTCTAC CAGCTCCACC AACCAAAACA TGTTCTGTGA 
     5581 GGAGCGGGTT GGCTACAACT GGGCCTACAA CACCATGCAC GCGTACGCGG CCGCTCGAGC 
     5641 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5701 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5761 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5821 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5881 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5941 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     6001 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     6061 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     6121 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     6181 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6241 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6301 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6361 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6421 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6481 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6541 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6601 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6661 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6721 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6781 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6841 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6901 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6961 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     7021 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     7081 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     7141 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     7201 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7261 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7321 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7381 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7441 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7501 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7561 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7621 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7681 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7741 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7801 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7861 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7921 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7981 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     8041 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     8101 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     8161 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     8221 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8281 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8341 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8401 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8461 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8521 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8581 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8641 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8701 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8761 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8821 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8881 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8941 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     9001 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     9061 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     9121 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     9181 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9241 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9301 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9361 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9421 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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