LOCUS       MR212011                9372 bp    DNA     circular  SYN 
DEFINITION  Col5a2 (Myc-DDK-tagged) - Mouse collagen, type V, alpha 2 (Col5a2)
ACCESSION   NM_007737
VERSION     NM_007737.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5529..5620 
                     /label="Myc-DDK"  
     misc_feature    5650..6235 
                     /label="PolyA signal"  
     misc_feature    5707..5726 
                     /label="Restriction Enzyme"  
     misc_feature    6511..7183 
                     /label="ColE1"  
     misc_feature    7613..8407 
                     /label="Kan/Neo"  
     misc_feature    8442..8744 
                     /label="SV40 ori"  
     misc_feature    8830..9307 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5511..5517 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5511 
                     /label="Col5a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGCAAAC TGGGTGGGAG CAAGACCTCT CCTCATTCTT TCTGTTTTAT 
     1081 TAGGGTACTG TGTCTCAATA AAAGCCCAGG AACAAGAGAA TGATGAATAT GATGAAGAAA 
     1141 TAGCCTGTAC TCAACATGGA CAGATGTATT TAAATAGAGA CATTTGGAAA CCTTCTCCAT 
     1201 GTCAGATCTG TGTCTGTGAC AATGGTGCCA TTCTTTGTGA CAAGATAGAG TGCCCAGAGG 
     1261 TGCTCAACTG TGCCAATCCT ATAACACCCA CTGGGGAGTG CTGTCCGGTG TGCCCACAAA 
     1321 CTGGAGGTGG AGATACTAGT TTTGGTAGAG GAAGAAAGGG ACAAAAAGGA GAACCAGGAT 
     1381 TAGTGCCTGT TGTAACAGGT ATACGCGGTC GTCCAGGACC TGCAGGCCCC CCAGGATCAC 
     1441 AAGGACCAAG AGGGGACCGA GGCCCTAAAG GAAGACCTGG TCCTCGTGGT CCTCAGGGAA 
     1501 TTGATGGAGA GCCAGGTGTG CCTGGTCAGC CAGGTGCTCC AGGGCCTCCT GGGCACCCCT 
     1561 CTCACCCAGG ACCTGATGGC ATGAGCAGGC CTTTTTCAGC TCAGATGGCT GGGCTGGATG 
     1621 AAAAATCTGG ACTTGGAAGT CAAGTAGGAC TCATGCCTGG CTCTGTGGGT CCTGTTGGCC 
     1681 CAAGAGGACC TCAGGGTTTA CAAGGACAGC AAGGTGGTGT GGGACCCGCA GGACCTCCTG 
     1741 GTGAGCCTGG CGAGCCTGGA CCAATGGGTC CAATTGGTTC TCGTGGACCA GAGGGCCCAC 
     1801 CTGGGAAGCC CGGAGAAGAT GGTGAACCTG GAAGAAATGG GAATACAGGT GAAGTGGGAT 
     1861 TCTCAGGATC ACCAGGTGCT CGAGGCTTCC CTGGTGCTCC TGGTCTCCCT GGCTTGAAAG 
     1921 GTCACAGAGG ACACAAAGGT CTTGAAGGCC CCAAAGGTGA AATTGGAGCA CCTGGTGCTA 
     1981 AGGGTGAAGC TGGCCCCACT GGTCCAATGG GTGCTATGGG CCCTCTGGGA CCAAGAGGAA 
     2041 TGCCAGGAGA GAGGGGAAGA CTTGGGCCAC AGGGTGCTCC TGGAAAACGG GGTGCCCACG 
     2101 GTATGCCTGG AAAACCTGGA CCAATGGGTC CTCTTGGGAT ACCTGGCTCT TCCGGTTTTC 
     2161 CAGGAAATCC TGGAATGAAG GGAGAAGCAG GTCCTACTGG AGCCCGAGGC CCTGAAGGTC 
     2221 CTCAGGGGCA GAGAGGTGAA ACTGGGCCAC CTGGTCCAGC TGGCTCTCAG GGTCTTCCTG 
     2281 GTGCAGTGGG GACTGATGGT ACACCTGGCG CCAAAGGCCC CACAGGCTCT GCAGGAACCT 
     2341 CTGGCCCTCC TGGCTTGGCA GGGCCCCCTG GGTCTCCTGG CCCTCAAGGC AGTACTGGAC 
     2401 CTCAGGGAAT CCGAGGACAA TCGGGTGATC CAGGAGTTCC AGGTTTCAAA GGAGAAGCTG 
     2461 GTCCAAAGGG AGAACCAGGG CCACATGGTA TTCAGGGTCC CATTGGTCCA CCTGGTGAAG 
     2521 AAGGCAAAAG AGGTCCTCGG GGTGATCCAG GCACAGTTGG TCCTCCAGGA CCAATGGGAG 
     2581 AAAGGGGCGC TCCTGGCAAT CGTGGTTTTC CAGGGTCTGA TGGTTTACCT GGACCAAAGG 
     2641 GTGCTCAGGG AGAGCGTGGT CCTGTAGGTT CTTCGGGGCC TAAAGGAGGC CAGGGAGATC 
     2701 CAGGACGTCC AGGAGAACCT GGGCTTCCAG GTGCTCGGGG TCTGACAGGA AATCCTGGTG 
     2761 TTCAAGGTCC TGAAGGAAAA CTTGGGCCTT TGGGTGCTCC TGGGGAAGAT GGTCGGCCAG 
     2821 GTCCTCCAGG CTCCATTGGG ATCAGAGGAC AGCCTGGAAG CATGGGCCTT CCAGGCCCCA 
     2881 AAGGTAGCAG TGGTGACCTT GGAAAACCAG GAGAAGCAGG AAATGCTGGT GTGCCTGGGC 
     2941 AAAGGGGGGC TCCTGGCAAA GATGGAGAAG TTGGTCCTTC AGGCCCTGTG GGACCTCCGG 
     3001 GCCTAGCTGG GGAAAGAGGA GAGCAGGGAC CTCCAGGCCC CACTGGCTTT CAGGGACTTC 
     3061 CTGGACCTCC AGGGCCACCT GGAGAAGGTG GAAAGGCTGG TGATCAAGGT GTTCCTGGAG 
     3121 AGCCTGGAGC AGTTGGCCCA TTAGGACCTC GGGGAGAAAG AGGGAATCCT GGAGAAAGAG 
     3181 GAGAGCCAGG GATAACTGGA CTGCCTGGGG AGAAAGGCAT GGCTGGAGGA CATGGCCCTG 
     3241 ATGGTCCAAA GGGCAACCCA GGTCCAACTG GGACCATTGG AGACACAGGG CCACCAGGTC 
     3301 TTCAGGGCAT GCCAGGAGAA AGAGGAATTG CAGGAACACC TGGCCCTAAG GGTGACCGAG 
     3361 GTGGCATAGG AGAGAAAGGT GCTGAAGGAA CAGCAGGAAA TGACGGGGCA AGGGGTCTTC 
     3421 CAGGTCCCTT GGGACCTCCA GGTCCTGCAG GCCCTACTGG AGAAAAGGGT GAACCTGGTC 
     3481 CTAGAGGCTT AGTTGGCCCT CCTGGCTCCC GTGGCAATCC TGGTTCTCGT GGTGAAAATG 
     3541 GTCCAACTGG AGCTGTTGGT TTTGCTGGAC CCCAGGGTCC CGATGGACAG CCTGGTGTCA 
     3601 AGGGTGAGCC TGGAGAGCCA GGACAGAAGG GAGATGCTGG GTCTCCTGGA CCCCAAGGGC 
     3661 TAGCAGGATC ACCTGGCCCC CATGGTCCAC ATGGTGTTCC TGGACTGAAA GGCGGTAGAG 
     3721 GAACCCAGGG TCCACCTGGT GCTACAGGGT TTCCTGGTTC TGCTGGCAGA GTTGGGCCTC 
     3781 CAGGCCCTGC TGGAGCTCCA GGACCTGCAG GGCCCGCAGG AGAACCTGGG AAGGAAGGAC 
     3841 CTCCTGGTCT TCGTGGTGAC CCTGGCTCTC ATGGGCGAGT GGGAGATAGA GGACCAGCTG 
     3901 GCCCACCTGG TAGCCCAGGA GATAAAGGGG ACCCAGGAGA AGATGGACAA CCTGGTCCAG 
     3961 ATGGTCCTCC TGGTCCCGCT GGTACCACTG GGCAAAGAGG AATTGTTGGA ATGCCCGGAC 
     4021 AACGTGGGGA ACGAGGAATG CCTGGCCTTC CTGGCCCAGC GGGTACACCA GGAAAAGTAG 
     4081 GACCAACTGG TGCGACAGGA GATAAAGGTC CCCCTGGACC TGTAGGACCT CCAGGTTCCA 
     4141 ATGGTCCTGT AGGAGAACCT GGGCCAGAAG GTCCGGCTGG TAATGATGGT ACCCCAGGAC 
     4201 GGGATGGTGC TGTTGGAGAA CGGGGTGACC GTGGAGACCC CGGGCCTGCT GGTCTACCAG 
     4261 GCTCTCAGGG TGCCCCTGGA ACTCCTGGCC CTGTTGGTGC CCCTGGAGAT GCAGGACAAA 
     4321 GAGGAGAGCC AGGTTCTCGA GGCCCTGTAG GACCACCTGG TCGAGCTGGA AAGCGTGGTT 
     4381 TACCTGGACC ACAAGGACCT CGAGGTGACA AGGGTGACAA TGGAGACAGA GGTGACCGAG 
     4441 GTCAGAAAGG CCACAGAGGG TTCACTGGCC TGCAGGGTCT TCCTGGACCT CCTGGTCCAA 
     4501 ATGGTGAACA AGGAAGTGCT GGCATTCCTG GTCCTTTCGG TCCAAGAGGT CCTCCAGGAC 
     4561 CAGTAGGTCC TTCAGGAAAG GAAGGAAACC CTGGGCCACT TGGACCAATT GGACCTCCTG 
     4621 GTGTGCGGGG CAGTGTAGGA GAAGCAGGAC CAGAGGGTCC TCCTGGTGAA CCTGGGCCTC 
     4681 CTGGCCCTCC AGGTCCCCCT GGCCATCTTA CTGCTGCTCT TGGTGATATC ATGGGACACT 
     4741 ATGATGAGAA CATGCCAGAT CCACTTCCAG AGTTTACGGA AGACCAGGCA GCTCCAGATG 
     4801 ACACAAACAA AACAGACCCT GGGATCCATG TTACCCTGAA GTCTCTCAGT AGTCAGATTG 
     4861 AAACCATGCG TAGCCCTGAT GGTTCCAAGA AACACCCAGC CCGCACTTGT GATGATTTAA 
     4921 AGCTTTGCCA TCCCACAAAG CAGAGCGGTG AATATTGGAT TGATCCTAAC CAGGGATCAG 
     4981 CTGAAGATGC AATCAAAGTT TACTGCAATA TGGAAACAGG AGAGACATGT ATTTCAGCAA 
     5041 ACCCAGCCAG TGTTCCACGT AAAACCTGGT GGGCCAGCAA ATCCCCTGAC AATAAGCCTG 
     5101 TGTGGTACGG TCTTGACATG AATAGAGGAT CTCAGTTCAC ATATGGAGAT TACCAGTCTC 
     5161 CTAACACAGC TATCACTCAG ATGACCTTTT TGCGTCTTTT ATCAAAAGAA GCCTCGCAGA 
     5221 ACCTTACTTA CATCTGTAGG AACACTGTTG GATATATGGA TGATCAAGCT AAAAACCTCA 
     5281 AGAAAGCTGT GGTCCTTAAA GGGTCAAACG ACTTAGAAAT AAAAGGAGAA GGAAACATCA 
     5341 GATTCAGATA CACAGTTCTT CAAGACACCT GCTCTAAGCG AAATGGAAAT GTAGGCAAAA 
     5401 CTATCTTTGA ATACAGAACA CAGAATGTGG CCCGCTTGCC CATCATAGAT GTTGGTCCTG 
     5461 TGGATATTGG CAATGCAGAC CAGGAATTTG GCCTTGATAT TGGGCCAGTT TGTTTCATGA 
     5521 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5581 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5641 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5701 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5761 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5821 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5881 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5941 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     6001 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     6061 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     6121 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6181 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6241 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6301 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6361 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6421 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6481 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6541 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6601 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6661 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6721 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6781 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6841 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6901 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6961 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     7021 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     7081 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     7141 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7201 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7261 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7321 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7381 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7441 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7501 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7561 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7621 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7681 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7741 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7801 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7861 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7921 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7981 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     8041 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     8101 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     8161 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8221 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8281 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8341 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8401 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8461 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8521 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8581 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8641 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8701 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8761 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8821 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8881 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8941 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     9001 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     9061 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     9121 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9181 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9241 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9301 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9361 AACGCGAATT TT
//