LOCUS       MR212008                9342 bp    DNA     circular  SYN 
DEFINITION  Col2a1 (Myc-DDK-tagged) - Mouse collagen, type II, alpha 1 (Col2a1), transcript variant 1
ACCESSION   NM_031163
VERSION     NM_031163.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5499..5590 
                     /label="Myc-DDK"  
     misc_feature    5620..6205 
                     /label="PolyA signal"  
     misc_feature    5677..5696 
                     /label="Restriction Enzyme"  
     misc_feature    6481..7153 
                     /label="ColE1"  
     misc_feature    7583..8377 
                     /label="Kan/Neo"  
     misc_feature    8412..8714 
                     /label="SV40 ori"  
     misc_feature    8800..9277 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5481..5487 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5481 
                     /label="Col2a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCCGCCTC GGGGCTCCCC AGTCGCTGGT GCTGCTGACG CTGCTCATCG 
     1081 CCGCGGTCCT ACGGTGTCAG GGCCAGGATG CCCAGGAGGC TGGCAGCTGT CTGCAGAATG 
     1141 GGCAGAGGTA TAAAGATAAG GATGTATGGA AGCCCTCATC TTGCCGCATC TGTGTGTGTG 
     1201 ACACTGGGAA TGTCCTCTGC GATGACATTA TCTGTGAAGA CCCAGACTGC CTCAACCCCG 
     1261 AGATCCCCTT CGGAGAGTGC TGTCCCATCT GCCCAGCTGA CCTCGCCACT GCCAGTGGAA 
     1321 AATTAGGGCC AAAGGGGCAG AAAGGAGAAC CTGGAGATAT CAGAGATATC ATAGGACCCA 
     1381 GAGGACCTCC TGGCCCTCAG GGACCTGCAG GTGAACAAGG ACCCAGAGGT GATCGTGGTG 
     1441 ACAAGGGAGA AAAGGGTGCG CCTGGACCCC GTGGCAGAGA TGGAGAACCT GGTACCCCTG 
     1501 GAAATCCTGG CCCCGCTGGC CCTCCAGGTC CCCCTGGTCC CCCTGGCCTT AGTGCAGGAA 
     1561 ACTTCGCGGC TCAGATGGCT GGAGGGTATG ACGAGAAGGC TGGTGGTGCC CAGATGGGAG 
     1621 TCATGCAAGG GCCCATGGGC CCCATGGGAC CCCGTGGACC CCCAGGCCCT GCCGGTGCCC 
     1681 CCGGCCCTCA AGGATTTCAA GGCAATCCTG GTGAACCTGG CGAGCCTGGT GTCTCTGGTC 
     1741 CCATGGGTCC CCGAGGTCCT CCTGGCCCTG CTGGAAAACC TGGTGACGAC GGTGAAGCTG 
     1801 GGAAGCCCGG AAAGTCTGGG GAAAGAGGCC TCCCTGGCCC TCAGGGTGCT CGTGGATTCC 
     1861 CAGGAACCCC GGGTCTCCCC GGTGTCAAGG GTCACAGAGG TTACCCAGGC CTCGACGGTG 
     1921 CTAAGGGGGA AGCTGGTGCT CCGGGTGTGA AGGGTGAGAG TGGTTCCCCT GGTGAGAACG 
     1981 GATCCCCGGG CCCAATGGGT CCCCGTGGCC TGCCTGGTGA GAGAGGACGG ACTGGCCCTG 
     2041 CTGGTGCTGC TGGTGCTCGG GGTAACGATG GCCAGCCAGG CCCCGCTGGA CCTCCGGGTC 
     2101 CTGTGGGTCC CGCAGGTGGT CCTGGCTTCC CTGGTGCTCC TGGTGCCAAG GGCGAAGCTG 
     2161 GTCCCACTGG TGCTCGCGGT CCTGAAGGTG CTCAAGGTTC TCGTGGCGAG CCTGGCAATC 
     2221 CTGGGTCCCC TGGGCCTGCA GGTGCTTCTG GTAACCCAGG GACTGATGGT ATTCCTGGAG 
     2281 CCAAAGGATC CGCTGGTGCT CCTGGAATTG CTGGTGCCCC TGGCTTCCCT GGGCCCCGTG 
     2341 GCCCTCCCGG TCCTCAAGGT GCAACTGGTC CCCTTGGCCC CAAAGGTCAG GCGGGTGAAC 
     2401 CTGGCATTGC TGGCTTTAAA GGTGATCAAG GCCCCAAGGG AGAGACTGGA CCTGCTGGGC 
     2461 CCCAAGGAGC CCCTGGCCCC GCTGGTGAAG AAGGCAAACG AGGTGCTCGA GGAGAGCCGG 
     2521 GTGGTGCTGG ACCAATCGGA CCCCCTGGAG AGAGAGGTGC TCCTGGCAAC CGTGGATTCC 
     2581 CAGGTCAAGA TGGTCTGGCA GGTCCCAAGG GTGCCCCTGG AGAGCGAGGG CCCAGTGGCT 
     2641 TGACTGGTCC CAAGGGAGCC AACGGTGACC CGGGTCGTCC TGGAGAACCT GGTCTTCCTG 
     2701 GAGCCAGGGG TCTTACCGGT CGCCCTGGTG ACGCTGGTCC TCAAGGCAAA GTTGGTCCTT 
     2761 CTGGAGCCCC TGGTGAAGAC GGTCGCCCTG GACCTCCTGG TCCTCAGGGA GCTCGTGGGC 
     2821 AGCCTGGCGT CATGGGTTTC CCTGGCCCCA AAGGTGCCAA CGGCGAGCCT GGCAAAGCTG 
     2881 GTGAGAAGGG TCTGGCTGGC GCTCCTGGTC TGAGAGGTCT TCCTGGAAAA GACGGTGAGA 
     2941 CGGGAGCCGC AGGACCCCCC GGCCCCAGTG GACCTGCTGG TGAACGAGGC GAGCAGGGCG 
     3001 CTCCTGGACC ATCAGGGTTC CAGGGACTTC CTGGCCCTCC CGGTCCCCCA GGTGAAGGTG 
     3061 GAAAGCAAGG TGACCAGGGT ATTCCTGGTG AAGCTGGAGC TCCTGGCCTT GTGGGTCCTC 
     3121 GGGGCGAGCG AGGTTTCCCA GGTGAACGTG GCTCTCCTGG TGCTCAGGGC CTTCAGGGTC 
     3181 CCCGAGGCCT CCCTGGCACT CCTGGTACTG ATGGTCCCAA AGGTGCAGCT GGCCCAGATG 
     3241 GCCCCCCTGG GGCTCAGGGG CCTCCAGGTC TACAGGGAAT GCCTGGTGAG AGAGGAGCCG 
     3301 CTGGCATTGC TGGGCCCAAG GGAGACAGAG GCGATGTTGG CGAGAAAGGC CCAGAGGGAG 
     3361 CTCCTGGGAA GGATGGCGGC CGAGGTCTGA CTGGGCCCAT CGGACCCCCA GGCCCAGCAG 
     3421 GGGCCAACGG CGAGAAGGGA GAAGTCGGAC CTCCTGGCCC GTCAGGAAGT ACCGGAGCTC 
     3481 GAGGTGCCCC GGGTGAACGC GGAGAGACCG GGCCACCTGG ACCTGCTGGA TTCGCTGGCC 
     3541 CTCCTGGTGC TGATGGCCAG CCTGGTGCCA AGGGTGATCA AGGAGAAGCC GGACAGAAAG 
     3601 GAGATGCTGG TGCCCCCGGC CCACAAGGCC CCTCGGGAGC CCCTGGGCCA CAGGGTCCTA 
     3661 CTGGAGTGAC TGGTCCTAAG GGAGCCCGAG GTGCCCAAGG TCCCCCGGGA GCCACCGGAT 
     3721 TCCCTGGAGC TGCTGGCCGA GTTGGACCCC CAGGTGCTAA TGGCAATCCT GGACCCGCCG 
     3781 GTCCCCCTGG TCCTGCTGGA AAAGATGGTC CCAAAGGTGT TCGAGGAGAC AGTGGCCCCC 
     3841 CTGGCAGAGC TGGTGACCCC GGTCTTCAAG GTCCTGCAGG AGCTCCTGGC GAGAAAGGAG 
     3901 AACCTGGAGA TGATGGTCCC TCTGGTCTTG ATGGTCCTCC AGGTCCCCAG GGGCTGGCTG 
     3961 GTCAAAGGGG CATTGTTGGT CTGCCTGGTC AGCGTGGTGA GAGAGGATTC CCCGGCCTTC 
     4021 CCGGCCCATC GGGTGAGCCC GGCAAGCAGG GTGCACCTGG CGCGTCTGGA GACAGAGGTC 
     4081 CTCCTGGTCC TGTGGGGCCT CCTGGCCTGA CAGGGCCTGC AGGTGAACCT GGACGAGAGG 
     4141 GCAGCCCTGG TGCTGATGGA CCCCCTGGAA GAGATGGTGC AGCTGGAGTC AAGGGAGATC 
     4201 GTGGTGAGAC TGGAGCACTG GGTGCCCCTG GAGCTCCTGG GCCCCCAGGC TCTCCTGGTC 
     4261 CTGCTGGCCC AACTGGCAAA CAAGGAGACA GAGGAGAGGC TGGTGCACAA GGTCCTATGG 
     4321 GTCCCTCAGG ACCTGCTGGA GCCCGTGGGA TTGCAGGCCC TCAAGGCCCC CGAGGTGACA 
     4381 AAGGAGAATC TGGAGAGCAG GGCGAGAGGG GACTGAAGGG ACACCGAGGT TTCACTGGAC 
     4441 TGCAGGGTCT GCCTGGCCCT CCGGGTCCTT CTGGAGATCA GGGTGCTTCT GGCCCTGCTG 
     4501 GTCCTTCTGG CCCTAGAGGT CCACCTGGCC CTGTTGGTCC CTCTGGCAAA GATGGCTCTA 
     4561 ATGGAATCCC TGGCCCCATC GGGCCTCCAG GTCCCCGTGG ACGCTCAGGA GAAACAGGCC 
     4621 CTGTTGGTCC CCCTGGAAGT CCCGGTCCTC CTGGCCCTCC AGGTCCTCCT GGTCCTGGCA 
     4681 TCGACATGTC AGCCTTTGCT GGCTTAGGGC AGAGAGAGAA GGGCCCCGAC CCCATGCAGT 
     4741 ACATGCGGGC CGACGAGGCA GACAGTACCT TGAGACAGCA CGACGTGGAG GTGGACGCTA 
     4801 CACTCAAGTC ACTGAACAAC CAGATTGAGA GCATCCGCAG CCCCGACGGC TCCCGCAAGA 
     4861 ACCCTGCTCG CACTTGCCAA GACCTGAAAC TCTGCCACCC CGAGTGGAAG AGCGGAGACT 
     4921 ACTGGATTGA TCCCAACCAG GGCTGCACCT TGGACGCCAT GAAAGTTTTC TGCAACATGG 
     4981 AGACCGGCGA GACTTGCGTC TACCCCAACC CAGCGACTGT CCCTCGGAAA AACTGGTGGA 
     5041 GCAGCAAGAG CAAGGAAAAG AAACACATCT GGTTTGGAGA GACCATGAAC GGTGGCTTCC 
     5101 ACTTCAGCTA TGGCGATGGC AACCTGGCTC CCAACACCGC TAACGTCCAG ATGACTTTCC 
     5161 TCCGTCTACT GTCCACTGAG GGCTCCCAGA ACATCACCTA CCACTGTAAG AACAGCATCG 
     5221 CCTACCTGGA CGAAGCGGCT GGCAACCTCA AGAAGGCCTT GCTCATCCAG GGCTCCAATG 
     5281 ATGTAGAGAT GAGGGCCGAG GGCAACAGCA GGTTCACATA CACTGCCCTG AAGGATGGCT 
     5341 GCACGAAACA CACTGGTAAG TGGGGCAAGA CCGTCATCGA GTACCGATCA CAGAAGACCT 
     5401 CCCGCCTTCC CATTATTGAC ATCGCACCCA TGGACATTGG AGGGGCTGAA CAGGAATTTG 
     5461 GTGTGGACAT AGGGCCTGTC TGCTTCTTGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5521 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5581 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5641 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5701 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5761 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5821 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5881 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5941 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     6001 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     6061 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     6121 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6181 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6241 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6301 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6361 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6421 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6481 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6541 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6601 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6661 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6721 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6781 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6841 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6901 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6961 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     7021 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     7081 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     7141 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7201 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7261 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7321 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7381 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7441 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7501 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7561 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7621 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7681 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7741 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7801 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7861 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7921 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7981 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     8041 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     8101 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     8161 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8221 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8281 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8341 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8401 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8461 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8521 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8581 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8641 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8701 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8761 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8821 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8881 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8941 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     9001 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     9061 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     9121 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9181 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9241 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9301 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//