LOCUS       MR212004                9303 bp    DNA     circular  SYN 
DEFINITION  A2m (Myc-DDK-tagged) - Mouse alpha-2-macroglobulin (A2m)
ACCESSION   NM_175628
VERSION     NM_175628.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5460..5551 
                     /label="Myc-DDK"  
     misc_feature    5581..6166 
                     /label="PolyA signal"  
     misc_feature    5638..5657 
                     /label="Restriction Enzyme"  
     misc_feature    6442..7114 
                     /label="ColE1"  
     misc_feature    7544..8338 
                     /label="Kan/Neo"  
     misc_feature    8373..8675 
                     /label="SV40 ori"  
     misc_feature    8761..9238 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5442..5448 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5442 
                     /label="A2m"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGAAGCGC TGGCTCCCAA GCCTGGCCCT GCTGCCGCTG CCGCCACCGC 
     1081 TGCTGCTGCT GCTGTTGCTG CTGCTGCCCA CAAATGCCTC AGCACCACAG AAACCAATCT 
     1141 ACATGGTGAT GGTTCCCTCC CTGCTCCACG CTGGCACCCC TGAGAAGGGC TGCCTCCTGT 
     1201 TCAACCACCT AAACGAGACA GTAACAGTGA AAGTATCCAT GGAGTCTGTC CGTGGGAACC 
     1261 AAAGCCTCTT CACTGACCTT GTTGTTGACA AGGACTTATT CCACTGTGCC TCCTTCATCG 
     1321 TCCCACAGTC TTCATCCAAT GAGGTGATGT TTCTCACTGT CCAAGTAAAA GGACCAACTC 
     1381 ATGAGTTCAG GAGGAGGAGC ACGGTGCTGA TCAAGACTAA AGAGAGCCTG GTCTTTGCTC 
     1441 AGACTGACAA GCCCATCTAC AAACCAGGAC AGATGGTGAG ATTTCGTGTT GTCTCATTGG 
     1501 ACGAAAATTT CCACCCCCTT AATGAATTGA TTCCTCTACT GTACATTCAG GATTCAAAAA 
     1561 AAAATCGCAT TGCACAGTGG CAGAATTTCC GCTTAGAGGG TGGCCTCAAA CAGCTGTCCT 
     1621 TCCCCCTCTC CTCAGAGCCC ACTCAGGGCT CCTACAAGGT GGTGATACGT ACAGAATCAG 
     1681 GCAGGACCGT CGAGCACCCC TTCTCTGTGA AGGAATTTGT GCTTCCCAAG TTTGAAGTCA 
     1741 AAGTTGCAGT ACCAGAGACA ATCACCATCC TGGAGGAAGA GATGAATGTG TCCGTGTGTG 
     1801 GCATATACAC CTATGGGAAG CCTGTTCCAG GACACGTGAC TGTGAACATT TGCAGAAAGT 
     1861 ACAGTAATCC TTCTAGCTGC TTTGGTGAAG AATCGCTGGC TTTCTGTGAG AAATTCAGTC 
     1921 AACAGCTAGA TGGCCGTGGC TGCTTCTCAC AGCTAGTGAA AACCAAGTCT TTCCAGCTAA 
     1981 AGAGGCAAGA GTATGAGATG CAGCTTGATG TGAATGCCAA GATCCAAGAA GAAGGAACAG 
     2041 GTGTGGAAGA AACCGGTAAA GGGCTCACTA AGATCACAAG AACTATAACA AAACTATCAT 
     2101 TTGTGAACGT GGATACACAT TTCAGACAAG GAATTCCTTT CGTTGGGCAG GTGCTCCTGG 
     2161 TGGATGGGAG AGGCACCCCT ATTCCATATG AAATGATCTT CATTGGAGCT GATGAAGCAA 
     2221 ACCAGAACAT AAATACTACC ACTGATAAGA ATGGCCTGGC ACGGTTCTCC ATCAACACTG 
     2281 ATGACATCAT GGGCACGTCC CTAACTGTCA GGGCCAAATA CAAAGATAGC AATGTCTGCT 
     2341 ATGGATTTAG ATGGTTGACA GAAGAGAACG TAGAGGCTTG GCACACTGCC AACGCTGTCT 
     2401 TCTCACCGAG CAGAAGCTTC GTGCACCTGG AATCCCTGCC CTATAAACTG CGCTGTGAGC 
     2461 AAACCCTGGC GGTCCAGGCT CATTATATTC TAAATGACGA GGCTGTGCTG GAGCGGAAGG 
     2521 AGTTGGTCTT CTATTATCTG ATGATGGCAA AGGGAGGCAT TGTTCGGGCA GGGACTCACG 
     2581 TTCTGCCTGT GACGCAGGGA CACAAGAAAG GCCATTTCTC CATCCTCATC TCGATGGAGA 
     2641 CAGACCTGGC TCCTGTAGCT CGACTGGTCC TTTATACCAT TCTGCCCAAT GGAGAAGTGG 
     2701 TTGGAGATAC TGTTAAATAT GAGATTGAAA AGTGCCTGGC CAACAAGGTG GATTTGGTCT 
     2761 TCCACCCAAA TATCGGTCTT CCGGCCACAC GTGCCTTCCT CAGTGTCATG GCTTCTCCTC 
     2821 AGTCTCTCTG TGGCCTGCGA GCTGTGGACC AAAGCGTACT GCTCACAAAG CCTGAGGCCG 
     2881 AACTCTCTGC ATCCCTGGTT TATGATCTGC TACCAGTGAA AGACCTCACT GGCTTCCCTA 
     2941 AGGGTGTGAA TCAACAGGAA GAAGACACTA ATGGCTGCCT TAAGCAAAAT GACACTTACA 
     3001 TTAATGGAAT CCTGTACTCC CCAGTACAGA ATACAAATGA AGAGGACATG TATGGCTTCC 
     3061 TAAAGGATAT GGGCTTAAAG GTATTCACCA ACTTGAACAT CCGTAAACCC AAAGTCTGTG 
     3121 AACGCCTTGG AGTCAATAAA ATACCAGCTG CATACCACCT TGTAAGCCAA GGCCACATGG 
     3181 ATGCTTTTCT AGAGTCTTCA GAGTCTCCCA CAGAGACTAC ACGAAGCTAC TTCCCTGAGA 
     3241 CGTGGATCTG GGACTTGGTG ATCGTGGACT CAACAGGAGT GGCTGAAATG GAAGTGACAG 
     3301 TCCCTGACAC CATCACTGAA TGGAAGGCTG GGGCCTTCTG CCTGTCCAAT GACACTGGGT 
     3361 TGGGCCTCTC TCCTGTGATC GATTTCCAAG CCTTCCAGCC CTTCTTCGTG GATCTCACAA 
     3421 TGGCCTACTC CGTGATCCGT GGAGAGGCCT TCACGCTCAA GGCCACTGTG CTGAACTACC 
     3481 TCCAGACATG CATCCGGGTT GGTGTACAGC TGGAGGCCTC TCCTGATTTC CTAGCTACCC 
     3541 CAGAGGAGAA GGAACAAAAG TCTCACTGCA TCTGTATGAA CGAGCGGCAT ACCATGTCCT 
     3601 GGGCGGTGAT CCCCAAGTCA TTAGGAAATG TGAATTTCAC AGTGAGTGCA GAGGCACTGG 
     3661 ACTCTAAGGA GCTGTGTAGG AATGAGGTGC CAGTGGTTCC TGAACGTGGA AAGAAAGACA 
     3721 CGATCATCAA GTCCCTGCTG GTTGAACCTG AAGGTCTAGA GAATGAAGTG ACATTCAACA 
     3781 GTTTGCTTTG TCCAACAGGT GCTGAGGTAT CGGAACAGAT ATCTCTGAAA CTGCCATCAG 
     3841 ATGTGGTAGA GGAATCTGCC CGAGCTTCTG TCACAGTTTT GGGAGATATA TTGGGTTCTG 
     3901 CTATGCAGAA CACACAGGAT CTCCTTAAGA TGCCCTATGG CTGTGGAGAA CAGAACATGG 
     3961 TTCTGTTTGC TCCTAATATC TATGTCCTGG ACTATCTGAA TGAAACAGAG CAGCTGACAC 
     4021 AGGAGATCAA GACCAAGGCC ATTACCTATC TCAATACGGG TTACCAAAGA CAATTAAACT 
     4081 ACAAGCACCG GGATGGCTCC TACAGTACTT TTGGGGATAA ACCTGGTAGG AGTCATGCCA 
     4141 ATACCTGGCT CACAGCCTTT GTACTGAAGA GTTTTGCTCA GGCTCGAAGA TATATCTTCA 
     4201 TCGATGAATC ACACATCACC CAAGCCCTCA CCTGGCTCTC CCAGCAGCAG AAGGACAATG 
     4261 GCTGCTTCAG GAGCTCCGGG TCACTGCTCA ACAATGCCAT GAAGGGAGGA GTAGAAGATG 
     4321 AAGTCACTTT GTCTGCCTAC ATCACCATTG CTCTCCTGGA GATGTCTCTT CCTGTCACTC 
     4381 ATCCTGTTGT CCGAAATGCC CTCTTTTGCC TGGACACAGC CTGGAAGTCA GCGAGGAGAG 
     4441 GAGCCAGCGG CAACCATGTC TACACTAAGG CACTGTTGGC CTATGCATTT GCCCTTGCTG 
     4501 GTAACCAGGA CACGAAGAAG GAGATACTGA AATCACTCGA TGAGGAGGCG GTAAAAGAAG 
     4561 ACAATTCTGT CCACTGGACC AGAGCTCAGA AACCCAGAGT GCCAGCAGAT CTCTGGTACC 
     4621 AACCCCAGGC TCCCTCGGCT GAGGTAGAGA TGACCGCTTA TGTGCTCCTG GCTTATCTTA 
     4681 CCACTGAGCT GGTTCCAACC CGAGAGGACC TGACTGCTGC AATGCTCATT GTGAAGTGGC 
     4741 TCACAAAGCA GCAGAATTCC CACGGTGGCT TCTCCTCCAC CCAGGACACG GTAGTGGCTC 
     4801 TCCATGCTTT GTCCAAATAC GGAGCAGCTA CTTTCACAAG AGCGAAGAAA GCTGCACATG 
     4861 TGACCATCCA GTCTTCTGGC GCATTTTATA CAAAATTCCA AGTGAACAAC GACAACCAGT 
     4921 TATTATTACA GAGAGTCACA TTGCCAACTG TGCCTGGGGA TTACACCGCA AAGGTAGCTG 
     4981 GAGAAGGGTG TGTCTACCTC CAGACATCCT TGAAATATAG TGTTCTCCCG AGGGAGAAGG 
     5041 AGTTTCCATT TGCTTTGGTG GTGCAGACTC TGCCTGGGAC TTGTGAGGAT CTCAAAGCTC 
     5101 ACACCACCTT CCAGATCTCA CTCAATATCA GTTACATTGG AAGCCGTTCT GACTCCAACA 
     5161 TGGCAATTGC TGATGTGAAG ATGGTGTCTG GCTTCATCCC CTTGAAACCA ACAGTGAAAA 
     5221 TGCTTGAAAG ATCTGTGCAT GTGAGCCGGA CGGAAGTCAG CAATAACCAT GTCTTGATTT 
     5281 ACTTGGATAA GGTGTCAAAT CAGATGCTGA CCTTGTTCTT CATGGTGCAG CAAGATATTC 
     5341 CAGTGAGAGA CCTGAAGCCA GCCATAGTGA AAGTCTATGA TTACTACGAG AAAGATGAGT 
     5401 TTGCAGTTGC AAAATACAGT GCTCCCTGCA GTGCAGGTTA TGGAAATGCC ACGCGTACGC 
     5461 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5521 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5581 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5641 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5701 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5761 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5821 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5881 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5941 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     6001 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     6061 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6121 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6181 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6241 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6301 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6361 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6421 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6481 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6541 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6601 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6661 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6721 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6781 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6841 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6901 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6961 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     7021 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     7081 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7141 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7201 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7261 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7321 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7381 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7441 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7501 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7561 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7621 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7681 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7741 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7801 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7861 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7921 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7981 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     8041 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     8101 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8161 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8221 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8281 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8341 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8401 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8461 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8521 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8581 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8641 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8701 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8761 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8821 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8881 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8941 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     9001 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     9061 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9121 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9181 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9241 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9301 TTT
//