LOCUS       MR212000                9276 bp    DNA     circular  SYN 
DEFINITION  Neo1 (Myc-DDK-tagged) - Mouse neogenin (Neo1), transcript variant 2
ACCESSION   NM_001042752
VERSION     NM_001042752.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5433..5524 
                     /label="Myc-DDK"  
     misc_feature    5554..6139 
                     /label="PolyA signal"  
     misc_feature    5611..5630 
                     /label="Restriction Enzyme"  
     misc_feature    6415..7087 
                     /label="ColE1"  
     misc_feature    7517..8311 
                     /label="Kan/Neo"  
     misc_feature    8346..8648 
                     /label="SV40 ori"  
     misc_feature    8734..9211 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5415..5421 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5415 
                     /label="Neo1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGAG CGCGAAGCCG GGCGACTCCT CTGCACCTCC TCCTCCCGGC 
     1081 GCTGCTGTCC GCCACCGCCG CTGCTGCTGT TGCTGCCGCT GCTGCTGCTG CTCGGACGCC 
     1141 CGGCGTCCGG CGCCGCGGCC ACGAAGAGCG GCTCCCCGCC GCAGTCCGCA GGAGCCAGTG 
     1201 TTCGAACATT CACTCCGTTT TATTTTCTGG TGGAGCCAGT AGACACCCTC TCAGTTAGAG 
     1261 GCTCTTCTGT TATATTAAAT TGCTCGGCAT ATTCTGAGCC CTCTCCAAAC ATTGAATGGA 
     1321 AGAAAGATGG GACTTTTTTA AACTTAGAAT CAGATGATCG ACGCCAGCTA CTCCCAGATG 
     1381 GATCTTTATT CATCAGCAAC GTGGTGCATT CCAAACACAA TAAGCCTGAC GAAGGTTTCT 
     1441 ATCAGTGTGT AGCCACTGTG GATAATCTTG GAACCATTGT CAGCAGAACA GCCAAGCTCA 
     1501 CAGTAGCAGG TCTTCCAAGA TTTACCAGCC AACCAGAACC TTCTTCAGTC TATGTTGGAA 
     1561 ACAGTGCAAT TCTGAATTGT GAAGTTAATG CAGATTTGGT CCCATTTGTT AGGTGGGAAC 
     1621 AGAATCGACA GCCCCTTCTT CTAGATGACA GGATTGTCAA ACTTCCAAGT GGAACACTGG 
     1681 TTATCAGCAA TGCTACTGAA GGAGATGGGG GACTCTACCG CTGCATTGTT GAAAGTGGTG 
     1741 GGCCACCAAA GTTTAGTGAC GAAGCTGAAT TGAAAGTTCT TCAAGATCCT GAGGAAATTG 
     1801 TAGACTTGGT ATTTCTGATG CGACCATCTT CTATGATGAA AGTCACTGGT CAGAGTGCAG 
     1861 TGTTGCCATG TGTTGTCTCA GGGCTTCCTG CTCCAGTTGT TAGATGGATG AAAAACGAAG 
     1921 AAGTGCTTGA CACAGAAAGC TCTGGCAGGT TGGTCTTGCT AGCAGGAGGT TGCTTGGAGA 
     1981 TCAGTGATGT CACTGAGGAT GATGCTGGGA CTTATTTTTG CATAGCTGAT AATGGAAATA 
     2041 AGACAGTTGA AGCTCAGGCG GAGCTTACTG TGCAAGTGCC ACCTGGATTC CTGAAACAAC 
     2101 CTGCTAACAT ATATGCTCAC GAATCCATGG ACATTGTATT TGAATGTGAA GTCACTGGGA 
     2161 AGCCAACTCC AACTGTGAAG TGGGTCAAGA ATGGGGATGT GGTTATCCCC AGTGATTACT 
     2221 TTAAAATTGT AAAGGAACAT AATCTTCAAG TTTTGGGTCT GGTGAAATCA GATGAAGGGT 
     2281 TCTATCAATG CATTGCTGAG AATGATGTTG GAAATGCACA AGCTGGAGCC CAGCTGATAA 
     2341 TCCTTGAGCA TGATGTTGCC ATCCCAACAT TACCTCCCAC TTCACTGACC AGTGCCACTA 
     2401 CTGACCATCT AGCACCAGCC ACAACGGGAC CATTACCTTC AGCTCCTCGA GACGTCGTGG 
     2461 CCTCCCTGGT CTCTACTCGC TTCATTAAAT TGACATGGCG TACACCTGCA TCAGACCCTC 
     2521 ATGGAGACAA TCTCACCTAC TCTGTGTTCT ACACCAAGGA AGGGGTTGCT AGGGAGCGTG 
     2581 TTGAGAATAC CAGCCAGCCA GGAGAGATGC AGGTGACTAT TCAAAACTTG ATGCCAGCAA 
     2641 CTGTGTACAT CTTCAAAGTT ATGGCTCAAA ATAAGCATGG CTCTGGAGAA AGTTCAGCTC 
     2701 CTCTTCGAGT AGAGACACAG CCTGAGGTTC AGCTCCCTGG CCCAGCACCT AATATCCGTG 
     2761 CTTATGCAAC GTCACCTACT TCTATCACTG TCACCTGGGA AACACCGTTA TCTGGCAATG 
     2821 GGGAAATTCA AAATTACAAA TTGTACTACA TGGAAAAAGG AACTGATAAA GAACAGGATA 
     2881 TTGATGTTTC AAGTCACTCC TACACCATTA ATGGACTGAA GAAATACACA GAATACAGTT 
     2941 TCCGAGTGGT GGCCTACAAT AAACATGGTC CTGGAGTTTC TACACAAGAT GTTGCTGTTC 
     3001 GAACATTATC AGATGTTCCC AGTGCTGCTC CTCAGAATCT GTCCTTAGAA GTGAGAAATT 
     3061 CAAAGAGTAT AGTGATCCAC TGGCAGCCCC CTTCCTCAAC CACACAAAAT GGGCAGATAA 
     3121 CTGGCTACAA GATTCGATAT CGAAAGGCCT CCCGAAAAAG TGATGTCACT GAGACCTTGG 
     3181 TAACTGGGAC ACAGCTGTCT CAGCTGATTG AAGGTCTTGA TCGGGGGACA GAATATAACT 
     3241 TCCGAGTCGC TGCTCTCACA GTCAATGGTA CAGGTCCAGC AACTGATTGG CTGTCTGCTG 
     3301 AAACTTTTGA AAGCGACCTA GATGAAACTC GTGTTCCTGA AGTGCCCAGC TCTCTTCATG 
     3361 TCCGTCCGCT CGTCACTAGC ATTGTAGTGA GCTGGACTCC TCCAGAGAAC CAGAACATTG 
     3421 TGGTCCGAGG TTATGCCATC GGTTACGGCA TTGGCAGCCC TCATGCCCAG ACCATCAAAG 
     3481 TGGACTATAA ACAACGTTAT TACACCATCG AAAACTTGGA TCCAAGCTCT CATTACGTGA 
     3541 TTACCTTGAA AGCATTTAAC AATGTTGGCG AAGGCATCCC CCTTTATGAG AGTGCTGTGA 
     3601 CCAGACCTCA CACAGTGCCA GATCCCACTC CCATGATGCC ACCAGTGGGA GTTCAGGCTT 
     3661 CCATTCTGAG TCACGACACC ATAAGGATTA CCTGGGCAGA CAACTCCCTG CCCAAACACC 
     3721 AGAAGATTAC AGACTCCCGC TACTACACAG TCCGGTGGAA GACCAACATC CCAGCAAACA 
     3781 CGAAGTACAA GAATGCAAAT GCAACGACGT TAAGCTATTT GGTTACTGGT TTAAAGCCAA 
     3841 ATACGCTCTA TGAGTTCTCT GTGATGGTGA CCAAAGGCAG AAGGTCAAGC ACGTGGAGTA 
     3901 TGACAGCTCA TGGCGCTACC TTTGAATTAG TTCCTACTTC TCCACCTAAG GATGTGACAG 
     3961 TTGTGAGTAA GGAAGGAAAA CCTAGAACCA TCATAGTGAA TTGGCAGCCT CCCTCTGAAG 
     4021 CTAACGGCAA GATTACAGGT TACATCATCT ATTACAGCAC GGATGTGAAT GCAGAGATAC 
     4081 ATGACTGGGT TATTGAACCA GTTGTGGGAA ACAGACTGAC TCACCAGATT CAAGAGTTAA 
     4141 CACTTGATAC GCCATACTAC TTCAAAATCC AGGCCCGGAA CTCAAAGGGC ATGGGGCCCA 
     4201 TGTCTGAAGC TGTACAGTTC AGAACACCTA AAGCCTTAGG GTCAGCAGGA AAAGGAAGCC 
     4261 GACTACCAGA CCTGGGATCT GACTACAAAC CTCCAATGAG TGGCAGCAAC AGCCCTCACG 
     4321 GGAGCCCCAC CTCCCCTCTG GACAGCAACA TGCTGCTGGT CATCATTGTC TCTGTTGGCG 
     4381 TCATCACTAT CGTGGTGGTT GTGGTCATTG CTGTCTTTTG TACCCGGCGC ACCACCTCTC 
     4441 ACCAGAAGAA GAAACGAGCT GCGTGCAAAT CAGTGAATGG CTCCCATAAG TACAAGGGCA 
     4501 ATTGCAAAGA TGTGAAGCCT CCAGACCTAT GGATCCATCA CGAGAGACTA GAGTTGAAGC 
     4561 CTATTGACAA GTCTCCAGAT CCTAACCCTG TCATGACTGA TACTCCAATC CCTCGAAACT 
     4621 CTCAAGATAT CACACCAGTG GACAATTCCA TGGATAGCAA TATCCATCAA AGGCGGAATT 
     4681 CATACAGAGG GCATGAGTCA GAGGACAGCA TGTCTACACT GGCTGGAAGG AGGGGAATGA 
     4741 GACCAAAAAT GATGATGCCC TTTGACTCTC AGCCACCTCA GCCTGTGATT AGTGCCCATC 
     4801 CCATCCATTC CCTCGATAAC CCTCACCATC ATTTCCACTC CAGCAGCCTC GCTTCTCCAG 
     4861 CCCGCAGTCA TCTCTACCAC CCAAGCAGCC CATGGCCCAT TGGCACATCC ATGTCCCTTT 
     4921 CAGACAGGGC CAATTCCACA GAATCTGTTC GAAATACCCC CAGCACGGAC ACCATGCCAG 
     4981 CGTCCTCGTC TCAGACGTGC TGCACTGACC ATCAGGACCC TGAGGGTGCT ACTAGCTCCT 
     5041 CTTACTTGGC CAGCTCCCAA GAGGAAGACT CAGGCCAGAG TCTTCCCACA GCCCATGTCC 
     5101 GCCCTTCCCA CCCTCTGAAG AGCTTCGCTG TGCCAGCAAT CCCACCCCCA GGACCTCCTC 
     5161 TCTATGATCC TGCACTGCCA AGCACACCAT TACTGTCCCA GCAAGCTCTG AACCATCACA 
     5221 TTCACTCAGT GAAAACAGCC TCCATCGGGA CGTTAGGAAG GAGCCGGCCT CCTATGCCAG 
     5281 TGGTTGTTCC GAGTGCCCCT GAAGTACAGG AGACCACCAG GATGCTGGAA GACTCCGAGA 
     5341 GTAGCTATGA ACCAGATGAG CTGACCAAAG AGATGGCCCA CCTGGAAGGA CTAATGAAGG 
     5401 ACCTAAATGC CATCACAACA GCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5461 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5521 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5581 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5641 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5701 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5761 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5821 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5881 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5941 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     6001 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     6061 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     6121 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     6181 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6241 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6301 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6361 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6421 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6481 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6541 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6601 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6661 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6721 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6781 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6841 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6901 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6961 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     7021 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     7081 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     7141 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     7201 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7261 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7321 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7381 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7441 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7501 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7561 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7621 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7681 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7741 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7801 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7861 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7921 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7981 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     8041 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     8101 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     8161 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     8221 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8281 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8341 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8401 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8461 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8521 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8581 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8641 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8701 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8761 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8821 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8881 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8941 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     9001 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     9061 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     9121 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     9181 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9241 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//