LOCUS       MR211998                9273 bp    DNA     circular  SYN 
DEFINITION  Col3a1 (Myc-DDK-tagged) - Mouse collagen, type III, alpha 1 (Col3a1)
ACCESSION   NM_009930
VERSION     NM_009930.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5430..5521 
                     /label="Myc-DDK"  
     misc_feature    5551..6136 
                     /label="PolyA signal"  
     misc_feature    5608..5627 
                     /label="Restriction Enzyme"  
     misc_feature    6412..7084 
                     /label="ColE1"  
     misc_feature    7514..8308 
                     /label="Kan/Neo"  
     misc_feature    8343..8645 
                     /label="SV40 ori"  
     misc_feature    8731..9208 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5412..5418 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5412 
                     /label="Col3a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGAGCTTT GTGCAAAGTG GAACCTGGTT TCTTCTCACC CTTCTTCATC 
     1081 CCACTCTTAT TTTGGCACAG CAGTCCAACG TAGATGAATT GGGATGCAGC CACCTTGGTC 
     1141 AGTCCTATGA GTCTAGAGAT GTCTGGAAGC CAGAACCATG TCAAATATGT GTCTGTGACT 
     1201 CAGGATCTGT CCTTTGCGAT GACATAATCT GTGATGAGGA GCCACTAGAC TGCCCCAACC 
     1261 CAGAGATCCC ATTTGGAGAA TGTTGTGCAA TATGCCCACA GCCTTCTACA CCTGCTCCTG 
     1321 TGCTTCCTGA TGGCCATGGA CCTCAAGGCC CCAAGGGAGA TCCGGGTCCT CCTGGCATTC 
     1381 CTGGGAGAAA TGGTGACCCT GGCCTTCCAG GACAACCAGG TCTCCCTGGG CCTCCTGGCT 
     1441 CCCCTGGAAT CTGTGAATCA TGTCCAACTG GTGGCCAAAA TTATTCTCCC CAATTCGACT 
     1501 CATATGATGT CAAGTCTGGA GTGGGAGGAA TGGGTGGCTA TCCGGGACCA GCTGGTCCAC 
     1561 CAGGCCCTCC AGGACCCCCT GGTTCTTCTG GACATCCTGG ATCTCCTGGT TCTCCTGGAT 
     1621 ACCAAGGTCC CCCTGGTGAA CCTGGTCAAG CTGGTCCGGC AGGCCCTCCA GGACCTCCTG 
     1681 GTGCTCTTGG TCCAGCTGGT CCTGCTGGAA AGGATGGAGA GTCAGGAAGA CCTGGACGAC 
     1741 CTGGAGAGCG TGGACTGCCT GGACCTCCAG GTATCAAAGG CCCAGCTGGC ATGCCTGGAT 
     1801 TCCCTGGTAT GAAAGGACAC AGAGGCTTTG ATGGACGCAA TGGAGAAAAG GGAGAAACAG 
     1861 GTGCTCCTGG ACTGAAGGGT GAAAATGGTC TTCCAGGAGA CAACGGAGCT CCTGGCCCCA 
     1921 TGGGTCCTAG AGGGGCTCCT GGTGAGCGAG GACGACCAGG CCTTCCTGGA GCTGCAGGTG 
     1981 CTCGAGGCAA TGATGGTGCT CGGGGCAGTG ATGGGCAACC TGGTCCCCCT GGCCCTCCTG 
     2041 GAACTGCAGG ATTCCCTGGA TCCCCTGGTG CTAAGGGTGA AGTTGGACCT GCAGGGTCCC 
     2101 CTGGCTCAAA TGGCTCACCA GGACAAAGAG GGGAGCCTGG ACCGCAGGGA CATGCTGGTG 
     2161 CTCAAGGGCC TCCTGGCCCT CCCGGGAATA ACGGCAGTCC TGGTGGAAAG GGTGAAATGG 
     2221 GTCCAGCTGG CATTCCTGGA GCCCCTGGAC TAATAGGAGC AAGGGGCCCC CCTGGACCAG 
     2281 CAGGAACTAA TGGTATACCC GGAACACGAG GTCCTTCAGG TGAACCCGGC AAGAACGGTG 
     2341 CGAAGGGAGA GCCAGGAGCA CGCGGTGAAC GGGGCGAAGC TGGTTCCCCA GGAATTCCAG 
     2401 GACCTAAGGG CGAAGATGGC AAAGATGGAT CACCTGGAGA ACCTGGTGCA AATGGGCTTC 
     2461 CAGGAGCCGC AGGAGAAAGG GGTCCTAGTG GCTTCCGAGG ACCTGCAGGG CCAAATGGCA 
     2521 TCCCAGGAGA AAAGGGTCCT CCCGGAGAGC GTGGTGGCCC AGGTCCTGCA GGACCCAGAG 
     2581 GAGTAGCTGG AGAACCTGGC CGAGATGGAA CCCCTGGAGG TCCAGGGATA CGGGGTATGC 
     2641 CCGGAAGTCC AGGTGGACCA GGCAATGATG GAAAACCAGG ACCTCCTGGA AGTCAAGGAG 
     2701 AAAGTGGTCG CCCTGGTCCC CCTGGCCCAT CTGGTCCCAG AGGTCAGCCT GGTGTCATGG 
     2761 GTTTCCCTGG TCCTAAAGGA AATGATGGTG CCCCTGGCAA GAATGGAGAA CGGGGTGGCC 
     2821 CTGGAGGACC TGGCCTTCCA GGTCCTGCTG GAAAGAATGG GGAGACTGGA CCTCAGGGTC 
     2881 CCCCAGGACC TACTGGCCCA GCTGGTGACA AGGGAGATTC TGGACCCCCT GGTCCACAAG 
     2941 GATTACAAGG CATACCTGGT ACCGGTGGTC CTCCAGGAGA AAATGGGAAA CCAGGTGAAC 
     3001 CAGGTCCAAA GGGTGAAGTC GGTGCTCCTG GAGCTCCTGG AGGCAAGGGT GATTCTGGTG 
     3061 CACCTGGAGA ACGTGGGCCT CCTGGAACAG CAGGAATCCC TGGTGCTAGA GGTGGAGCTG 
     3121 GACCCCCTGG CCCTGAGGGA GGAAAGGGCC CTGCTGGTCC CCCTGGCCCT CCTGGTGCTT 
     3181 CTGGTTCTCC TGGTCTGCAA GGGATGCCAG GGGAAAGAGG AGGTCCTGGA AGTCCTGGCC 
     3241 CAAAGGGTGA AAAGGGTGAA CCAGGTGGTG CAGGTGCTGA CGGAGTTCCA GGAAAGGATG 
     3301 GGCCAAGGGG TCCTGCTGGC CCTATTGGTC CCCCTGGCCC AGCTGGTCAG CCTGGAGATA 
     3361 AGGGTGAAGG TGGTTCCCCT GGACTTCCAG GTATAGCTGG ACCTCGAGGT GGCCCTGGTG 
     3421 AGAGAGGTGA ACATGGCCCT CCAGGACCTG CTGGCTTCCC GGGTGCTCCT GGACAGAATG 
     3481 GTGAACCAGG TGCTAAAGGA GAAAGAGGTG CCCCTGGAGA GAAAGGTGAA GGAGGCCCTC 
     3541 CAGGACCTGC AGGACCCACT GGCAGTTCTG GACCTGCTGG TCCGCCTGGT CCTCAGGGTG 
     3601 TAAAGGGTGA ACGTGGTAGT CCTGGTGGTC CTGGTACTGC TGGCTTTCCC GGTGGGCGTG 
     3661 GTCTTCCTGG TCCTCCTGGC AATAATGGTA ATCCAGGGCC CCCAGGACCC AGTGGTGCTC 
     3721 CTGGCAAGGA CGGCCCTCCA GGTCCTGCAG GCAACAGTGG TTCTCCTGGC AACCCTGGAA 
     3781 TAGCTGGACC AAAAGGTGAT GCTGGACAGC CTGGAGAGAA GGGGCCACCT GGTGCTCAGG 
     3841 GTCCTCCGGG TTCCCCAGGC CCACTTGGAA TTGCAGGGCT AACTGGAGCA CGAGGTCTTG 
     3901 CTGGACCACC AGGCATGCCA GGTCCACGGG GTAGCCCTGG ACCTCAGGGT ATCAAGGGTG 
     3961 AAAGTGGAAA ACCAGGAGCC AGTGGCCATA ATGGGGAACG TGGTCCTCCT GGACCCCAAG 
     4021 GTCTTCCTGG TCAGCCTGGT ACAGCTGGTG AACCTGGAAG GGATGGAAAC CCTGGATCAG 
     4081 ATGGTCAGCC AGGTCGAGAT GGATCTCCTG GTGGCAAGGG TGATCGTGGT GAAAATGGTT 
     4141 CTCCTGGTGC CCCAGGCGCT CCTGGTCATC CAGGCCCACC TGGCCCTGTT GGTCCATCTG 
     4201 GAAAGAGTGG TGACAGAGGA GAAACTGGCC CTGCTGGTCC TTCTGGTGCT CCAGGCCCTG 
     4261 CTGGAGCTCG AGGTGCTCCG GGTCCCCAAG GTCCACGAGG TGACAAAGGT GAAACTGGTG 
     4321 AACGTGGCTC TAATGGCATC AAAGGACATC GAGGATTCCC TGGCAATCCA GGTCCTCCAG 
     4381 GTTCTCCTGG TGCTGCTGGT CACCAGGGTG CAATTGGTAG TCCAGGACCT GCAGGTCCCA 
     4441 GAGGACCAGT TGGACCACAT GGACCTCCTG GAAAAGATGG AACAAGTGGG CATCCAGGTC 
     4501 CTATTGGACC ACCAGGTCCT AGAGGAAACA GAGGTGAAAG AGGATCTGAG GGCTCGCCAG 
     4561 GCCACCCTGG ACAGCCAGGA CCCCCTGGAC CCCCTGGTGC CCCTGGTCCC TGCTGTGGTG 
     4621 GTGGTGCTGC TGCCATTGCT GGAGTTGGAG GTGAAAAGTC TGGTGGCTTT TCACCCTATT 
     4681 ATGGAGACGA TCCAATGGAT TTCAAGATCA ACACTGAAGA GATTATGTCT TCACTCAAGT 
     4741 CTGTTAATGG ACAAATAGAG AGTCTTATAA GCCCTGATGG TTCTCGAAAA AACCCTGCTC 
     4801 GGAACTGCAG AGACCTAAAA TTCTGCCACC CCGAACTCAA GAGTGGAGAA TACTGGGTTG 
     4861 ATCCTAACCA AGGCTGCAAG ATGGATGCTA TAAAAGTATT CTGTAACATG GAAACTGGGG 
     4921 AAACATGCAT AAATGCCAGC CCCATGACTG TCCCACGTAA GCACTGGTGG ACAGATTCTG 
     4981 GTGCAGAGAA GAAACATGTT TGGTTTGGAG AATCTATGAA TGGTGGTTTT CAGTTCAGCT 
     5041 ATGGCCCTCC TGATCTTCCT GAAGATGTCG TTGATGTGCA GCTGGCATTC CTCAGACTTC 
     5101 TTTCCAGCCG GGCCTCCCAG AACATTACAT ACCACTGCAA AAACAGTATT GCCTATATGG 
     5161 ATCAGGCCAG TGGCAATGTA AAGAAGTCTC TGAAGCTGAT GGGATCAAAT GAAGGCGAAT 
     5221 TCAAGGCTGA AGGAAACAGC AAATTCACTT ACACAGTTCT AGAGGATGGC TGTACTAAAC 
     5281 ACACTGGGGA ATGGAGCAAG ACAGTCTTTG AATATCAAAC ACGCAAGGCA ATGAGACTAC 
     5341 CCATCATAGA TATCGCACCC TATGACATTG GGGGTCCTGA TCAAGAATTT GGTGTGGACA 
     5401 TTGGCCCTGT TTGCTTTTTA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5461 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5521 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5581 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5641 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5701 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5761 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5821 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5881 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5941 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6001 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6061 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6121 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6181 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6241 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6301 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6361 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6421 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6481 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6541 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6601 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6661 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6721 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6781 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6841 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6901 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6961 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7021 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7081 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7141 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7201 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7261 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7321 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7381 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7441 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7501 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7561 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7621 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7681 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7741 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7801 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7861 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7921 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7981 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8041 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8101 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8161 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8221 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8281 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8341 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8401 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8461 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8521 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8581 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8641 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8701 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8761 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8821 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8881 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8941 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9001 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9061 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9121 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9181 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9241 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//