LOCUS       MR211997                9267 bp    DNA     circular  SYN 
DEFINITION  Nup153 (Myc-DDK-tagged) - Mouse nucleoporin 153 (Nup153)
ACCESSION   NM_175749
VERSION     NM_175749.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5424..5515 
                     /label="Myc-DDK"  
     misc_feature    5545..6130 
                     /label="PolyA signal"  
     misc_feature    5602..5621 
                     /label="Restriction Enzyme"  
     misc_feature    6406..7078 
                     /label="ColE1"  
     misc_feature    7508..8302 
                     /label="Kan/Neo"  
     misc_feature    8337..8639 
                     /label="SV40 ori"  
     misc_feature    8725..9202 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5406..5412 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5406 
                     /label="Nup153"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCGGAA GCCGGAGGCA TAGGAGGGGG CGGCGGCGGC GGCAAGATCC 
     1081 GGACTCGTCG TTGCCACCAG GGGCCGGTGA AGCCTTACCA ACAGGGGCGA CCCCAACACC 
     1141 AGGGCATTCT TAGCAGGGTT ACAGAATCTG TTAAGAATAT TGTACCAGGG TGGCTACAAA 
     1201 GATACTTCAA CAAGAGTGAA AATGCTTGCA GCTGTTCACC AGATGCAGAT GAAGTTCCAC 
     1261 CCTGGCCAGA AAATAGAGAA GATGAGCATG CGATTTATGC GGATGAGAAC ACAAACACTG 
     1321 ATGATGGGAG AATCACCCCT GACCCTGCAG GCAGTAACAC AGAAGAACCA TCAACAACCA 
     1381 GTACTGCTTC AAATTATCCA GATGTGTTAA CAAGACCTTC TCTTCATCGG AGTCATCTGA 
     1441 ATTTCTCTGT GTTGGAATCC CCTGCTTTGC ACTGTCAGCC GTCCACATCC TCTGCGTTCC 
     1501 CAATCGGCAG TTCTGGATTT TCCCTTGTGA AGGAGATTAA AGATTCTACC TCTCAGCATG 
     1561 ATGATGATAA CATCTCAACT ACCAGTGGGT TCTCTTCAAG AGCTTCTGAG AAAGATATAG 
     1621 CTGTTTCAAA GAACACTTCC CTGCCACCTT TGTGGTCCCC TGAAGCAGAA CGTTCTCATT 
     1681 CACTCTCACA GCATACTGCC ATCAGCTCCA AAAAACCAGC ATTCAACTTG TCTGCCTTTG 
     1741 GAACACTTTC CACCTCACTT GGGAATTCTT CAATTCTTAA AACAAGTCAG CTTGGAGACT 
     1801 CTCCTTTTTA TCCTGGCAAA ACAACATATG GAGGGGCAGC TGCTGCTGTA AGACAGAATA 
     1861 AAGTACGAAG TACACCTTAC CAGGCTCCAG TTAGAAGACA GATGAAAGCT AAGCAGCTCA 
     1921 ATGCACAGTC TTATGGTGTG ACCAGCTCAA CAGCACGGCG GATACTGCAG TCATTAGAAA 
     1981 AGATGTCGAG CCCTCTTGCG GATGCAAAAA GAATTCCATC TGCAGTTTCT TCTCCTCTGA 
     2041 ATTCTCCTCT TGATAGGAGT GGGATAGATA ACACAGTTTT TCAGGCAAAA AAAGAAAAGG 
     2101 TGGACTCTCA GTATCCCCCT GTTCAGAGAC TAATGACCCC AAAGCCAGTT TCCATAGCAA 
     2161 CAAATAGAAC TGTCTATTTT AAACCATCTC TGACCCCATC TGGTGACTTA AGGAAGACTA 
     2221 ATCAAAGGAT AGATAAAAAG AACAGTACTG TAGATGAAAA AAGTATCTCA AGACAAAATA 
     2281 GAGAGCAAGA AAGTGGCTTT TCATACCCCA ATTTCAGTAT TCCTGCAGCC AATGGTTTAT 
     2341 CTTCTGGAGT AGGTGGTGGA GGTGGCAAGA TGAGAAGAGA GAGAACACAT TTTGTTGCTC 
     2401 CTAAACCTCC AGAGAATGAG GAAGTGGAAG CACCATTGTT ACCGCAAATC TCTCTACCAA 
     2461 TCAGCAGTTC TTCACTGCCC ACCTTCAGTT TTAGTTCCCC TGTGACCTCG GCTTCTCCTT 
     2521 CACCAGTCAG CTCTTCTCAG CCATTACCAA ACAAGGTACA GATGACCTCT CTGGGCAGCA 
     2581 CGGGCAGTCC TGTGTTCACA TTCTCATCTC CCATTGTAAA ATCCACGCAG GCAGCTGTGC 
     2641 TCCCTCCAGC ATCTATTGGA TTTACATTTA GTGTGCCTCT TGCAAAAACT GAATTTTCTG 
     2701 GCTCTAATAG TTCTTCAGAA ACAGTTTTAA GTAGTTCAGC CCAAGATATC ACTGCAGTGA 
     2761 ATAGTTCAAG CTATAAGAAG CGAAGTGCTC CTTGTGAGGA CCCCTTTACA CCTGCAAAGA 
     2821 TCCTGAGAGA AGGAAGTGTT CTAGACATTC TGAAAACACC TGGTTTCGCA TCACCAAAGG 
     2881 TTGATTCTCC TGCTCTTCAA CCCACTACCA CAAGCTCAAT AGTTTATACA AGACCAGCAA 
     2941 TAAGTACTTT TTCTTCTAGT GGAATTGAGT ATGGAGAAAG TTTAAAAGCT GGATCATCAT 
     3001 GGCAGTGTGA CACATGTCTC CTCCAGAACA AAGTCACAGA CAATAAGTGT ATAGCCTGCC 
     3061 AAGCAGCAAA ACTGCCCCTG AAAGAAACTG CTAAACAGAC TGGGACTGGG ACACCAAGCA 
     3121 AAAGTGACAA ACCAGCCTCT ACATCAGGGA CAGGCTTTGG AGATAAATTT AAACCAGCAA 
     3181 TAGGAACTTG GGATTGTGAT ACCTGTTTAG TGCAAAATAA GCCCGAAGCA GTAAAATGTG 
     3241 TAGCCTGTGA GACACCTAAG CCTGGCACTG GTGTGAAGCG AGCCCTTACA TTGACAGTGG 
     3301 CTTCAGAAAG TCCTGTGACT GCTTCCTCTT CTACCACTGT GACAACTGGT ACACTAGGAT 
     3361 TTGGAGATAA ATTCAAAAGG CCTGTGGGCT CTTGGGAGTG TCCAGTCTGC TGTGTTTGTA 
     3421 ATAAGGCAGA AGACAACAGA TGTGTTTCCT GTACATCTGA GAAACCAGGT TTGGTATCTG 
     3481 CCTCAAGCAG CAGCCCTGCC CCTGTCTCTC TGTCTTCTGG AGGCTGTTTA GGATTGGACA 
     3541 AGTTTAAAAA GCCCGAGGGA AGCTGGGACT GTGAAGTGTG CCTAGTGCAA AATAAAGCAG 
     3601 ACTCTGCCAA GTGCATAGCT TGTGAAAGTG CAAAGCCAGG CACAAAGTCT GAGTTTAAAG 
     3661 GCTTCGGGAC ATCTTCATCT TTAAATCCAG CACCCTCAGC CTTCAAATTT GGTATTCCAT 
     3721 CGTCCTCTTC TGGGCTTTCT CAAACTTTGA CAAGCACTGG AAATTTTAAA TTTGGAGATC 
     3781 AGGGAGGATT CAAATTAGGC ACTTCATCTG ACTCTGGGTC TACAAACACC ATGAATACAA 
     3841 ACTTTAAATT TTCTAAACCA ACTGGAGACT TTAAATTTGG AGTGTTATCT GATTCTAAAC 
     3901 CTGAAGAAGT TAAAAACGAC AACAAGAATG ATAATTTTCA GTTTGGATCT TCTTCTGGCT 
     3961 TAACCAACCC AGCTTCTTCA GCTCCGTTTC AGTTTGGGGT ATCTACTCTT GGGCAACAAG 
     4021 AAAAGAAAGA GGAGCTGCCT AAATCTTCAC CTGCAGGCTT TAGCTTTGGT GCAGGTGTTA 
     4081 ATAACCCCCC TAATGCTGCT ATTGACACCA CAGCGACCTC AGAGAACAAG AGCGGCTTCA 
     4141 ACTTTGGAAC CCTAGACACA AAGAGTGTCT CAGTGACTCC TTTCACATAC AAGACAACAG 
     4201 AAGCAAAGAA AGAAGACGCA CCTGCTACCA AGGGTGGGTT CACATTTGGT AAAGTGGGCT 
     4261 CCTCTTCTTT GCCATCTTCC TCAATGTTTG TTTTGGGAAG GACAGAGGAG AAACAGCAAG 
     4321 AACCTGTTAC TTCTACTTCT CTGGTGTTTG GGAAGAAAGC CGACAGTGAG GAGCCAAAGT 
     4381 GTCAGCCAGT ATTTTCCTTT GGGAATTCAG AGCAAACCAA AGATGAGAGC TCCAAGCCGA 
     4441 CATTCAGCTT CAGTGTGGCA AAACCATCTG GGAAGGAGTC TGAGCAGCTA GCCAAGGCTA 
     4501 CTTTTGCATT TGGAAATCAA ACCAATACAA CAACTGATCA AGGTGCAGCA AAGCCAGTTT 
     4561 TTAGCTTCTT GAACAGCAGT TCCTCTAGTT CAAGTGCACC AGCCACTTCA TCTAGTGGTG 
     4621 GCATATTTGG TAGTTCCACC TCTTCCTCCA ACCCACCTGT GGCAGCCTTT GTGTTTGGAC 
     4681 AGGCCAGCAA TCCTGTCAGC AGCTCTGCCT TCGGTAACGC TGCTGAATCC AGTACATCTC 
     4741 AGTCTTTGCT ATTTCCTCAA GAGAGCGAGC CAGCCACCAC GTCCAGCACT GCCCCAGCTG 
     4801 CCTCTCCCTT TGTATTTGGG ACAGGAGCCA GCAGTAATAG TGTGTCTTCT GGGTTCACTT 
     4861 TTGGGGCTAC AACCACGTCA AGCTCTTCAG GATCCTCCTT TGTATTTGGC ACTGGACATT 
     4921 CAGCACCATC TGCCAGTCCA GCATTTGGTG CTAACCAGAC TCCAACATTT GGACAAAGTC 
     4981 AAGGTGCCAG CCAGCCTAAC CCGCCAAGCT TTGGCTCTAT CTCGTCTTCA ACAGCATTAT 
     5041 TTTCTGCTGG TTCTCAGCCT GTACCACCAC CTATATTTGG GACAGTGTCA AGCAGCAGCC 
     5101 AACCTCCTGT GTTTGGACAG CAGCCTAGTC AGTCTGCATT TGGCTCTGGG ACAGCTAATG 
     5161 CCAGTTCGGT TTTCCAATTT GGTAGCAGCA CTACGAATTT CAACTTCACA AACAATAATC 
     5221 CATCAGGAGT GTTCACATTT GGTGCAAGTC CCAGCACGCC TGCAGCCTCA GCCCAGCCCT 
     5281 CAGGTTCAGG GGTCTTTTCA TTCAGCCAGT CTCCAGCATC ATTTACAGTG GGGTCAAATG 
     5341 GGAAAAATAT GTTCTCTTCT TCCGGAACTT CAGTTTCTGG TCGCAAGATA AAGACTGCTG 
     5401 TTAGACGCAA GAAAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5461 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5521 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5581 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5641 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5701 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5761 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5821 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5881 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5941 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6001 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6061 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6121 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6181 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6241 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6301 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6361 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6421 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6481 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6541 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6601 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6661 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6721 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6781 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6841 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6901 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6961 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7021 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7081 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7141 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7201 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7261 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7321 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7381 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7441 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7501 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7561 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7621 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7681 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7741 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7801 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7861 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7921 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7981 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8041 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8101 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8161 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8221 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8281 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8341 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8401 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8461 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8521 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8581 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8641 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8701 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8761 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8821 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8881 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8941 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9001 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9061 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9121 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9181 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9241 ATTTAACAAA AATTTAACGC GAATTTT
//