LOCUS       MR211990                9207 bp    DNA     circular  SYN 
DEFINITION  Cd109 (Myc-DDK-tagged) - Mouse CD109 antigen (Cd109)
ACCESSION   NM_153098
VERSION     NM_153098.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5364..5455 
                     /label="Myc-DDK"  
     misc_feature    5485..6070 
                     /label="PolyA signal"  
     misc_feature    5542..5561 
                     /label="Restriction Enzyme"  
     misc_feature    6346..7018 
                     /label="ColE1"  
     misc_feature    7448..8242 
                     /label="Kan/Neo"  
     misc_feature    8277..8579 
                     /label="SV40 ori"  
     misc_feature    8665..9142 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5346..5352 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5346 
                     /label="Cd109"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCAGTCGG AGGCTCCTGA GCGCGGCCCA CCTTCTCTGC TTGTGCGCGG 
     1081 TCGCGCTGGC CGCCCCAGGG TCCCGCTTTC TGGTGACAGC CCCAGGGATC ATCAGACCCG 
     1141 GAGCAAATGT GACTATTGGG GTGGATCTCC TGGAAAATAG CCCCCCACAG GTCCTTGTAA 
     1201 AGGCTCAGGT GTTCAAGATA GCTTCCAACA AATCGAGATC CATCCTGGAA GCAGAAGGAG 
     1261 TCTTTCACAG AGGCCATTTC AAGACTCTCG TTCTTCCGGC ACTACCTCTG AGCAGTGCAG 
     1321 ATAAGATTTA TGAGCTACAC ATAAACGGAC AATCAGAGAA TGAGATCGTA TTCTCCAACA 
     1381 GGACACGTTT AACATTTGAG AGCAAGAGTA TATCTGTCCT CATTCAGACA GACAAGGCAT 
     1441 TCTACAAGCC GAAGCAGGAA GTGAAGTTTC GGGTGCTCAC ACTCTGCTCA GATCTCAAGC 
     1501 CTTATAGGAC ATCCGTGGAC ATTTTCATTA AGGATCCGAA GTCTAATGTG ATCCAACAGT 
     1561 GGTTTTCTCA GAAAGGTGAT CTTGGAGTCG TTTCCAAAAC TTTTCAACTA TCCTCTAATC 
     1621 CAATATTTGG TGACTGGTCC ATTCAGGTTC AAGTGAATGA TCAGCAATAT TACCAGTCAT 
     1681 TTCAGGTCCT AGAATATGTG TTACCAAAGT TCGAAGTCAC CGTGCAGACC CCGCTGTATT 
     1741 GTTCCTTGAA GTCTAAACAG TTAAATGGCT CCGTCATAGC AAAGTATACA TACGGGAAGC 
     1801 CAGTGAAGGG AAGTCTGTCA CTTACATTTT TACCTTTGTC TTTTTGGGGA AAGAAGAAGA 
     1861 ATATCACAAA AAGTTTTGAG ATAAATGGAT TTGCAAACTT CTCTTTTGAT AATTATGAGA 
     1921 TGAAAAAGGT AATGAATTTG AAACCGCTAA CGGATGTCTC GGAAGGGAGT TATGAGAATG 
     1981 TGGACCCGTC CTTTCCTGGG CCGGCAGAGA TTATAGCTAC TGTGACGGAA TCACTCACAG 
     2041 GTATCTCAAG AATGGCAAGC ACCAATGTGT TTTTCAAGCA ACATGATTAC ATCATTGAGA 
     2101 TTTTTGATTA TACGACTGTC TTGAAACCAT CTCTCAACTT CACAGCCACT GTGAAGGTCA 
     2161 GCCGCTCTGA TGGCAATCAA CTAACTCCTG AAGAAATAGA AAATGATTTG GTCACAGTAG 
     2221 TGACCCAGCG GAAAAATAAC CACCCTGAGA GTCAGAGAGA TCAGGAAATG GATTATATCC 
     2281 AGACCGTAAA TTATACTATC CCCCAAAATG GAATCATTAA GATTGAATTC CCAGTCATGT 
     2341 CCATTTCCGG CGAGCTACAA CTGAAGGCTT ATTTTCTTGA TGGTACAAGT TCTGTGACAG 
     2401 TCCACAGTAT GTTCACCTCT CCTAGTAAGA CGTACATCCA GCTTAAAACC AGAGATGAAT 
     2461 ATATAAAGGT GGGGTCACCT TTTGATTTGA TGGTTAGTGG CAACAGACAA TTCAAGGACT 
     2521 TAAGCTATAT GGTAATATCA AAGGGACAGT TGGTGGCTGC AGGCAAACAA AGTTCAAGAA 
     2581 CTTTCTCTTT AACACCAGAG GCTTCCTGGG CTCCAAAGGC CTGTATAATC GCATATTATA 
     2641 TTGCAGAGGA TGGGGAAATT ATAAATGATA TTCTAAAAAT CCCCGTTCAG CTTGTTTTTG 
     2701 AAAATAAGGT AAAGCTGTTT TGGAGTAAAC CTACAGTCAA GCCATCTGAT AAGGTCTCTC 
     2761 TCAGGATCTC TGCGACACAG TCTGACTCCC TGGTGGGGAT TGTAGCTGTT GACAAAAGTG 
     2821 TAACATTGAT GGAAAACTCC AACAGTATTA CAATGGAAAC CATGGTCCAT GAACTGGAAC 
     2881 TTTATAACAC AGAATATTAT CTAGGCATGT TCATGAACTC CTTCGCTGTA TTTCAGGAAT 
     2941 GTGGTCTCTG GGTGTTGACA GATGCAACCC TTATAAGAGA TAGCATTGAT GAAGTCTACG 
     3001 ATACTGAAGA GTATTCTGAA CGCTTTGCAG AGGAAAATGA GGCAAACCTG GTTGATTTTG 
     3061 AAGATGCTTC TTCAGTTAAC AATGTACATG TCAGGAAGAA TTTTCCAGAA ACATGGATTT 
     3121 GGCTAGACGC CTACATGGGA TCTAAGATTT ATGAAGAATT TGAAGTTACT GTACCTGACT 
     3181 CCATCACTTC TTGGGTGGCT TCGGCTTTTG TCATCTCAGA AGACCTGGGT TTTGGGTTAA 
     3241 CAACCGTTCC AGCAGAGCTG CAAGCCTTCC AACCGTTTTT CCTTTTCCTG AATCTTCCGT 
     3301 ACTCTGTTAT CAGAGGTGAA GAGTTTGCTT TGGAAGTATC CATCGTCAAT TATTTGAAAG 
     3361 ATACAATTAA GGTTGTGATA CTCATTGAGG AAAGTGACAG CTTTGATATT TTAATGACTT 
     3421 CAAATGACAC CAATGGCACC ATATACCGGA AGACTGTTCA AGTTCCCAGG GATAATGGGG 
     3481 TCACTCTTGT TTTTCCCATC AAGCCCACGC ACTTGGGAGA GATTCCCATC ACAGTGACGG 
     3541 CTGCCTCACC CACTGCCTCT GATGCTGTCA CCCAGACAAT TGTAGTAAAG CCTGAAGGGA 
     3601 TAGAAAAGTC GTATTCGAAA TCTGTCTTAT TGGATCTGAC AGATAGCAAC GTAGAAAGTA 
     3661 AGCAGCAATC TATGAGATTC TCCTTTCCTC CTGATACGGT CATTGGCAGT GAAAGAGTTC 
     3721 AGATCACAGC AATTGGAGAT ATCCTTGGTT CCTCCATCAA TGGCTTATCT TCACTGATCC 
     3781 GGATGCCTTA TGGATGTGGT GAACAGAACA TGATATATTT TGCTCCAAAT ATTTACATTT 
     3841 TGGATTATCT GACTAAACAG AAACAGCTGA CAGTTAATTT AAAAGAAAAG GCCCTTTCAT 
     3901 ATATGAGGCA AGGTTACCAA AGGGAGCTTC TCTATCAGAG GGAAGATGGC TCCTTCAGTG 
     3961 CTTTTGGGGA CATTGACTCT TCTGGGAGCA CTTGGCTGTC AGCATTTGTT TTAAGATGCT 
     4021 TTCTGGAAGC TGATTACTAT ATAGATATTG ATCAGGATGT GTTACACAGA ACATATACTT 
     4081 GGCTTAATGC ACATAAGAAA TTCAATGGTG AATTTTGGGA GCCAGGAAGA GTGATTCACA 
     4141 GTGAACTTCA AGGTGGCACC AAAAGCCCAG TAACACTTAC GGCCTATATT GTGACTTCTG 
     4201 TCCTGGGATA CAAAAAGTAT CAGCCTAATA TCGATGTACA AGACTCAATC AAGTTTTTGG 
     4261 AATTTGAATT CAGCAGAGGA ATTTCGGACA ATTATACCTT AGCAATTATA TCCTATGCCC 
     4321 TGTCCACAGT CGGGAGCCCT AAAGCAGAGG AGGCTTTGAA CTTGCTGATG CAGCGATCAG 
     4381 AAAAGGAAGG AGACACACAG TTCTGGTTGT CATCAGGACC TGCGCTCTCT GGGTCCTGGC 
     4441 AGCCGCGCTC CGTGGACATC GAAATCGCTG CTTATGCACT GCTGGCGCAC ACTCTGCACC 
     4501 ATGTGTCTGA GGGAATCCCG GTTATGAGGT GGCTCATCCA GCAAAGAAAC AGCCTGGGAG 
     4561 GTTTTGTATC CACTCAGGAT ACTGTTGTGG CCCTGAAGGC ATTATCTGAA TTTTCAGCCC 
     4621 TTGTGCACAA GGAAAATACA GATATCCAAC TGACCGTCAC AGGGCCCGGC ATCCCCAGAT 
     4681 CCATACACTT CCGAATTGAC TCCCAGAACC TCTTTCTCCT TCACCAGGAA GAGCTTCATG 
     4741 CGCTAGATCC CATCACCGTT AATGTTTCTG CGCATGGCTC GGGATTTGCT ATTTGCCAGC 
     4801 TTAATGTTGA CTATAACGTG AAAGGTTCAG GTTCTTCTAA AAGACGGAGA TCTACCGAAA 
     4861 ATCAAGAAGT TTTTGATTTA GACGTCATTG TGAATAATGA GGACGACATT AGTCACCTGA 
     4921 ATCTGAATGT GTGCACAAGT CACTTGGGTT CAGAGAGGAC AGGCATGGTC CTTATGGAAG 
     4981 TGAACCTTCT CAGTGGCTTT AGTGCATCTT CAGATTCAAT TCCTCTGAGT GAGACCCTGA 
     5041 AGAAAGTGGA ATATGATAAC GGGAAACTCA ACCTTTATTT AGATTCTGTA AATGAATCCC 
     5101 AGTTTTGTGT TAATATTCCC ACTGTGAGAG ACTACAAAGT TTCAAATATT CGAGATGGTT 
     5161 CAGTATCTGT AATGGACTAC TATGAACCAA GGAGACAGGC AGTGCGAAGC TACAACACCC 
     5221 AGGTGAAGCT GTCCTCATGT TACCTCAGTC CAGACACCAA CTGCAAGTCA CACACGGACG 
     5281 GAGCCACGGA CTCCCTTCGA CGTTCTTCGA GCCTTCTCGT CTTTTGCTCC GTCCTTCTGT 
     5341 ACTTTGTGCA ACATACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5401 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5461 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5521 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5581 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5641 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5701 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5761 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5821 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5881 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5941 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6001 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6061 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6121 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6181 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6241 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6301 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6361 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6421 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6481 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6541 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6601 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6661 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6721 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6781 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6841 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6901 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6961 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7021 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7081 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7141 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7201 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7261 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7321 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7381 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7441 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7501 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7561 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7621 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7681 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7741 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7801 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7861 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7921 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7981 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8041 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8101 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8161 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8221 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8281 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8341 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8401 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8461 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8521 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8581 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8641 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8701 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8761 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8821 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8881 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8941 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9001 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9061 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9121 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9181 ATTTAACAAA AATTTAACGC GAATTTT
//