LOCUS       MR211986                9192 bp    DNA     circular  SYN 
DEFINITION  Synj2 (Myc-DDK-tagged) - Mouse synaptojanin 2 (Synj2), transcript variant 2
ACCESSION   NM_001113352
VERSION     NM_001113352.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5347..5438 
                     /label="Myc-DDK"  
     misc_feature    5468..6053 
                     /label="PolyA signal"  
     misc_feature    5525..5544 
                     /label="Restriction Enzyme"  
     misc_feature    6329..7001 
                     /label="ColE1"  
     misc_feature    7431..8225 
                     /label="Kan/Neo"  
     misc_feature    8260..8562 
                     /label="SV40 ori"  
     misc_feature    8648..9125 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5322..5329 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5322 
                     /label="Synj2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTAAGC AAAGGGCTGC GGCTGCTGGC GCGGCTGGAC CCCACTGGCC 
     1081 CGAGCAGCGT GCTGTTGGAG GCGCGGGGCC GCGGCGACTG CCTGCTCTTC GAGGCCGGCG 
     1141 CAGTGGCCAC GCTGGCTCCG GAAGAAAAGG AAGTCATTAA AGGACTGTAT AGCAAGCTGA 
     1201 CGGATGCCTA TGGCTGCCTC GGGGAGCTGA GGTTACAATC CGGTGGCGTC CCCTTGAGCT 
     1261 TTCTGGTGTT GGTGACAGGC TGCATGTCAG TGGGCAGAAT TCCAGATGCA GAGATCTACA 
     1321 AAATCACTGC CACTGAGTTG TACCCCCTGC AGGAAGAGGC CAAGGAAGAG GACCGCCTGC 
     1381 CCACCTTAAA GAAAATCCTG AGCTCAGGGG TGTTCTATTT CGCATGGCCC AATGATGGCG 
     1441 CCTGCTTCGA TCTGACCATC AGGGCTCAGA AACAGGGTGA TGACGGCTCT GAATGGGGGA 
     1501 CCTCTTTCTT CTGGAACCAG CTATTGCATG TGCCTCTGCG GCAGCACCAG GTGAACTGTC 
     1561 ATAACTGGTT GCTGAAAGTC ATCTGTGGGG TGGTGACCAT CCGCACAGTA TATGCCTCCC 
     1621 ACAAGCAGGC CAAGGCCTGT CTCATCTCTC GCATCAGCTG TGAACGCGCA GGTGCTCGCT 
     1681 TCCTCACCCG TGGTGTGAAC GATGATGGCC ACGTGTCCAA CTTTGTGGAG ACAGAGCAGA 
     1741 CGATTTACAT GGATGATGGA GTATCGTCCT TTGTCCAGAT CCGAGGCTCC GTTCCGCTGT 
     1801 TCTGGGAGCA ACCAGGACTT CAGGTTGGCT CCCATCATCT GAGACTGCAC AGAGGCCTAG 
     1861 AGGCCAACGC TCCTGCTTTT GAAAGGCACA TGGTGCTTCT GAAGGAGCAA TACGGTAAGC 
     1921 AGGTGGTTGT GAACCTGCTG GGTAGCAGAG GCGGTGAAGA GGTGCTCAAC AGAGCCTTCA 
     1981 AGAAGTTGCT CTGGGCTTCT TGCCACGCGG GTGACACACC TATGATAAAT TTTGACTTCC 
     2041 ATCAGTTTGC CAAAGGTAGG AAGCTAGAGA AATTGGAGAA CCTGTTGAGA CCTCAGTTAC 
     2101 AGCTACACTG GGAAGACTTC GGCGTGTTTG CGAAGGGCGA GAATGTAAGT CCACGGTTTC 
     2161 AGAAAGGCAC TCTGCGGATG AACTGTCTCG ACTGTCTGGA TAGAACCAAC ACTGTGCAGT 
     2221 GCTTCATTGC TCTTGAGGTC CTTCATCTGC AGCTTGAGAG CTTGGGGCTA AATTCAAAGC 
     2281 CCATCATTGA CCGTTTTGTG GAGTCCTTCA AAGCCATGTG GTCTCTGAAT GGGCACAGCC 
     2341 TGAGCAAGGT GTTCACAGGG AGCAGGGCCC TGGAAGGAAA GGCCAAGGTG GGAAAGCTGA 
     2401 AGGATGGGGC CCGATCCATG TCTCGCACCA TCCAGTCCAA CTTCTTCGAC GGGGTGAAGC 
     2461 AGGAGGCCAT CAAGCTACTG CTAGTCGGAG ATGTCTACAA TGAAGAGTCT ACAGACAAAG 
     2521 GACGGATGCT GCTGGACAAC ACGGCCCTTC TGGCGACCCC CAGGATCTTG AAGGCCATGA 
     2581 CAGAACGCCA GTCGGAATTC ACGAATTTCA AGCGCATCCA GATTGCTGTG GGGACCTGGA 
     2641 ATGTGAACGG AGGAAAGCAA TTCCGTAGCA ATCTCCTGGG GACGGCTGAG CTCACGGACT 
     2701 GGCTCCTAGA TGCTCCTCAG CTGTCAGGAG CAGTGGACTC CCAGGATGAT GGCAGTCCTG 
     2761 CTGACGTATT TGCCATCGGG TTTGAGGAGA TGGTGGAACT GAGTGCGGGA AATATTGTCA 
     2821 ATGCCAGCAC CACCAACAGG AAGATGTGGG GCGAGCAGCT TCAGAAAGCC ATCTCCCGCT 
     2881 CCCATCGGTA CATCCTCTTG ACCTCCGCAC AGCTGGTGGG CGTCTGTCTT TACATCTTTG 
     2941 TACGTCCGTA CCACGTCCCG TTCATCAGAG ACGTGGCCAT CGACACCGTG AAGACCGGCA 
     3001 TGGGGGGAAA GGCGGGGAAT AAGGGTGCTG TGGGCATCCG CTTCCAGCTC CACAGCACCA 
     3061 GTTTCTGCTT CGTCTGTAGC CACCTGACGG CTGGGCAGTC TCAGGTGAAG GAGAGGAATG 
     3121 AAGACTACCG GGAGATCACG CACAAACTCT CCTTCCCTTC GGGGAGAAAC ATATTTTCAC 
     3181 ATGATTACGT GTTTTGGTGT GGCGATTTCA ACTACCGTAT TGATCTTACT TACGAAGAAG 
     3241 TCTTCTATTT TGTTAAACGC CAAGACTGGA AGAAACTTAT GGAATTCGAT CAGTTACAGT 
     3301 TACAGAAATC AAGTGGAAAA ATTTTTAAAG ACTTTCATGA AGGAGCCGTT AACTTCGGAC 
     3361 CCACCTACAA GTATGACGTT GGATCAGCTG CCTACGACAC AAGTGACAAG TGCCGTACCC 
     3421 CAGCCTGGAC AGACAGGGTG CTGTGGTGGA GGAAGAAGCA TCCATATGAT AAAACAGCTG 
     3481 GTGAACTCAA CCTTCTAGAC AGCGATCTAG ACGGCGATCC CCAAATCAGA CACACCTGGT 
     3541 CTCCAGGCAC TCTGAAATAC TACGGCCGTG CAGAGCTGCA GGCGTCTGAT CACAGACCTG 
     3601 TGCTGGCCAT TGTGGAGGTG GAGGTTCAAG AGGTGGATGT AGGAGCCCGG GAGAGGGTCT 
     3661 TCCAGGAAGT GTCCTCTGTC CAAGGCCCGC TGGATGCCAC CGTTGTTGTA AACCTCCAGT 
     3721 CTCCAACCCT AGAAGAGAAA AATGAATTTC CAGAGGACCT GCGCACAGAA CTTATGCAGA 
     3781 CTTTGGGGAA TTATGGGACG ATTATTCTAG TCAGGATCAA CCAAGGGCAG ATGCTGGTGA 
     3841 CGTTTGCAGA CAGCCACTCG GCTCTCAGTG TGCTGGATGT GGATGGTATG AAGGTGAAAG 
     3901 GCAGGGCCGT GAAGATTCGA CCAAAGACCA AAGATTGGCT AGAAGGCCTG AGAGAGGAGC 
     3961 TTCTCCGGAA GCGAGACAGC ATGGCCCCTG TGTCTCCCAC CGCCAACTCC TGCTTGTTGG 
     4021 AGGAGAACTT TGACTTCTCG AGTCTGGACT ATGAGTCCGA AGGGGATGTT CTTGAAGAGG 
     4081 ATGAAGACTA TTTAGTGGAT GGGTTTGGCC AGCCTGTAGT CTCAGACAGT GAGCTCGGTG 
     4141 GAGACAACTC TTCCGACACC ATGAGCTCCT TGACACCCGC CAGCAAGTCT CCCGCCCTGG 
     4201 CTAAAAAGAA GCAACATCCA ACATACAAAG CTGGCTTAAT GGTGAAGAAG TCAGCCTCAG 
     4261 ACGCGTCCAT CTCTTCTGGC ACTCATGGAC AATATTCCAT CTTGCAGACA GCGAAACTTC 
     4321 TCCCAGGAGC ACCCCAGCAA CCACCCAAGG CTAGAACTGG AATAAGTAAA CCTTACAACG 
     4381 TCAAACAGAT CAAAACCACC AACGCTCAGG AGGCAGAAGC AGCTATCCGG TGTCTCCTGG 
     4441 AAGCTAGCGG AGGGGTCCCG GAATCAGCCC CAGGTGCCAT ACCCCTGAGA AACCAAGGGT 
     4501 CTTCTAAGCC AGAGGCCACC CTGGGGCCCC CAGCCCTGCC CCGCCGGCCT GCTCCAAGGG 
     4561 TTCCCACTAT GAAGAAACCA ACTTTGAGGA GGACAGGAAA GCCCATGTTA CCAGAAGAAA 
     4621 ACTTTGAGCC ACAGCCTGTC CATTTTACAA TGGCTTCCCA GGAAATGAAC CTTGAGACCC 
     4681 CTCCTCCAAT AACAGCTACT CCCATCCCGC CTGTTCCCAA ACCAAGAACA CTTCAGCCTG 
     4741 GGAAAGGTGT TGAGGGGAGG CCAAGCAGCG GTAAGCCAGA ACCAGATGAA GCTCCTTCCG 
     4801 TGACAGGCAC TGTGGAGTCA CCACCTCCAG AGGCCCAGGA GGCCCCGTCT CTGGCTCCCA 
     4861 AGGTGCCTCC GAGGAGGAAG AAGTCTGCAC CAGCGGCCTT CCACCTGCAG GTCCTGCAGA 
     4921 ACAACAGCCA AGTTCTCCAG GGCCTCACGT GCTCCAGCAG TTCTCCACCC TCACTGAAGC 
     4981 CCGACACCCA CCCGCTCTGT CTACAAGTGG CACTTGGCAC TTCATCTGCC AGAAGCCCTG 
     5041 AAACCCATGG CCCCAGAGTG ACAGAGCCCG AGGCGGCCTC TTTTCATGGC AACTATCCAG 
     5101 ATCCCTTCTG GAGCCTTCTC CACCACCCTA AGCTGTTGAA CAATACCTGG CTGTCCAAGA 
     5161 GCTCCGAGCC TTTAGACGTG GGGTCCAGGA ACCCTGAGAG GACACACACA GAGCCGGCAC 
     5221 AGGTCAATGC ATCGTTGGCT GAGAGGGGGC TTCCACCAGA CCATGGGGGA AAAGACTTAA 
     5281 GTCACTGGGT GACAGCTAGT AACAAAGACA AGAGGACAAC ATTAGGTGTT AGCGGACCGA 
     5341 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5401 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5461 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5521 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5581 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5641 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5701 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5761 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5821 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5881 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5941 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     6001 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6061 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6121 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6181 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6241 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6301 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6361 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6421 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6481 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6541 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6601 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6661 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6721 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6781 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6841 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6901 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6961 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     7021 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7081 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7141 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7201 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7261 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7321 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7381 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7441 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7501 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7561 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7621 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7681 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7741 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7801 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7861 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7921 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7981 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     8041 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8101 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8161 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8221 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8281 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8341 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8401 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8461 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8521 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8581 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8641 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8701 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8761 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8821 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8881 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8941 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     9001 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9061 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9121 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9181 AACGCGAATT TT
//