LOCUS       MR211975                9114 bp    DNA     circular  SYN 
DEFINITION  Eea1 (Myc-DDK-tagged) - Mouse early endosome antigen 1 (Eea1)
ACCESSION   NM_001001932
VERSION     NM_001001932.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5271..5362 
                     /label="Myc-DDK"  
     misc_feature    5392..5977 
                     /label="PolyA signal"  
     misc_feature    5449..5468 
                     /label="Restriction Enzyme"  
     misc_feature    6253..6925 
                     /label="ColE1"  
     misc_feature    7355..8149 
                     /label="Kan/Neo"  
     misc_feature    8184..8486 
                     /label="SV40 ori"  
     misc_feature    8572..9049 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5253..5259 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5253 
                     /label="Eea1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTTCGAAGG ATCTTGCAGA GGACTCCTGG GCGAGTTGGC TCTCAAGGTT 
     1081 CTGATTTAGA TTCATCAGCA ACTCCTATAA ATACGGTGGA TGTCAATAAT GAAAGTTCTT 
     1141 CAGAGGGTTT CATATGTCCC CAGTGTATGA AATCCCTTGG CTCTGCTGAT GAGCTTTTCA 
     1201 AGCACTATCA GGCCGTTCAT GATGCTGGTA ATGACTCGGG TCATGGTGGA GAGGCCGGTC 
     1261 TTGCTTTGAC ACGAGATGAC ATAACACTAC TCAGACAAGA GGTCCAAGAC CTACAGGCCT 
     1321 CACTTAAGGA AGAAAAATGG TACTCAGAAG AATTAAAGAA AGAATTAGAA AAATATCAAG 
     1381 GGCTACAGCA GCAAGAGGCT AAATCTGATG GATTGGTGAC TGATTCGTCA GCAGAGCTAC 
     1441 AGGCTTTAGA GCAACAATTA GAAGAAGCAC AAACAGAGAA TTTTAATATT AAGCAAATGA 
     1501 AAGATCTGTT TGAACAGAAA GCAGCCCAAC TTGCTACTGA AATTGCAGAT ATAAAGTCAA 
     1561 AATATGATGA AGAAAAGAGT CTCCGAGCAG CTGCTGAACA AAAAGTGACA CATTTGACAG 
     1621 AAGACTTAAA CAAACAGACA ACTGTAATTC AAGATCTGAA AACTGAACTG CTTCAGAGAC 
     1681 CTGGTATAGA AGATGTTGCT GTACTAAAGA AAGAACTGGT CCAAGTTCAA ACACTAATGG 
     1741 ATAACATGAC TTTGGAACGT GAGAGAGAAT CTGAAAAACT TAAAGATGAA TGCAAAAAGT 
     1801 TACAATCAGA GCATGCTCAT TTGGAGGCTA CAATAAACCA GCTAAGGAGT GAACTTGCCA 
     1861 AAGGCCCCCA AGAAGTTGCA GTATATGTGC AGGAAATACA AAAACTTAAA GGTTCAATTA 
     1921 ATGAATTAAC ACAAAAAAAC CAGAACTTGA CTGAAAAATT GCAGAAGAAA GACCTAGACT 
     1981 ACACCCACTT AGAGGAGAAG CACAATGAAG AGTCAGCAAG TCGGAAGACT CTCCAGGCAT 
     2041 CCCTCCACCA GCGGGACCTG GATTGCCAGC AGCTTCAGGC AAGGCTAACT GCATCTGAAA 
     2101 GCTCACTCCA GAGGGCCCAG GGAGAACTCA GTGAAAAGGC AGAGGCTGCT CAGAAGCTCA 
     2161 GAGAAGAACT GCGGGAAGTA GAGAGCACGC GCCAGCATCT CAAGGTGGAG GTGAAGCAGC 
     2221 TGCAGCAGCA GCGGGAAGAG AAGGAACAGC ACGGGCTGCA GCTGCAGGGG GAAGTCAGCC 
     2281 AGTTACACTG CAAACTTCTG GAGACCGAGC GCCAACTGGG GGAAGCTCAC GGCAGGCTGA 
     2341 AGGAACAGCG GCAGCTTTCC AGCGAGAAGC TGATGGAAAA GGAGCAGCAG GTGGCTGACC 
     2401 TACAGCTCAA GCTCTCTCGT CTGGAGGAGC AGTTGAAGGA AAAGGTAACA AATTCCACTG 
     2461 AATTACAACA TCAGTTAGAA AAATCAAAGC AACAGCATCA AGAACAGCAA GCTCTACAGC 
     2521 AAAGTGCTAC AGCCAAACTC CGAGAAGCTC AGAATGATTT GGAGCAAGTG TTACGTCAGA 
     2581 TTGGAGATAA GGACCAAAAG ATCCAGAACC TTGAAGCCTT GCTGCAGAAG GGTAAAGAAA 
     2641 GCGTTTCCTT ACTAGAAAAG GAAAGAGAAG ATCTTTATGC AAAAATCCAG GCCGGTGAAG 
     2701 GAGAGACTGC TGTTCTTAAC CAGTTACAAG AGAAAAATCA TGCACTGCAG CAGCAGCTAA 
     2761 CTCAACTGAC GGAGAAGCTG AAGAATCAGT CGGAAAGCCA TAAACAAGCC GAGGAGAACT 
     2821 TGCATGACCA GGTACAGGAA CAGAAGGCAC ATCTTCGAGC TGCACAAGAC CGTGTTCTTT 
     2881 CTCTGGAAAC CAGTGTCAGT GAACTAAGCA GTCAGTTAAA TGAAAGCAAG GAGAAGGTCT 
     2941 CGCAGCTTGA CATACAGATT AAAGCCAAAA CTGAATTATT ACTATCAGCA GAAGCAGCAA 
     3001 AAGCTGCTCA GAGAGCTGAT CTTCAGAATC ACTTGGACAC AGCTCAACAT GCGTTACAGG 
     3061 ATAAACAACA GGAGTTAAAT AAGGTCAGTG TGCAGTTGGA TCAGCTCACT GCAAAGTTTC 
     3121 AGGAGAAGCA GGAGCATTGC ATTCAGCTGG AAAGCCACCT TAAGGATCAC AAGGAGAAGC 
     3181 ACCTCTCTTT AGAACAAAAA GTTGAAGACT TAGAAGGCCA CATTAAGAAA CTAGAAGCTG 
     3241 ATGCTCTTGA AGTTAAAGCC AGCAAGGAAC AGGCTTTGCA GAGTCTACAG CAGCAGAGGC 
     3301 AGCTGAGCAC AGACCTGGAG TTGAGAAATG CAGAGCTGAG TAGAGAGCTC CAGGAGCAGG 
     3361 AGGAAGTAGT ATCTTGTACA AAACTGGATC TACAGAATAA ATCTGAAATC CTTGAAAATA 
     3421 TCAAACAAAC CCTCACCAAG AAAGAAGAAG AAAATGTCGT CTTGAAACAA GAATTTGAAA 
     3481 AGTTAAGTCA AGATTCAAAG ACACAGCACA AGGAGCTGGG GGACAGGATG CAAGCTGCGG 
     3541 TCACAGAGCT GACAGCAGTG AAGGCACAGA AGGACGCACT CCTGGCCGAG CTTTCTACAA 
     3601 CAAAAGAAAA ACTGTCAAAA GTTTCGGATT CTTTAAAAAA CTCCAAAAGT GAATTTGAGA 
     3661 AAGAAAATCA GAAAGGAAAA GCTGCTGTAT TAGATTTGGA AAAAGCTTGT AAAGAATTAA 
     3721 AGCATCAGCT TCAAGTACAG GCAGAGAGTG CCCTGAAGGA GCAGGAGGAC CTGAAAAAGT 
     3781 CACTCGAGAA AGAGAAAGAG ACTTCTCAGC AGTTGAAGAT AGAACTCAAT TCAGTCAAGG 
     3841 GAGAAGTCTC ACAGGCTCAA AATACATTAA AGCAAAAGGA GAAAGACGAA CAACAGCTTC 
     3901 AGGGGACCAT AAATCAGCTA AAGCAATCAG CTGAACAAAA GAAAAAACAA ATTGAAGCGC 
     3961 TTCAAGGAGA GGTTAAAAAT GCTGTTTCAC AAAAGACAGT GCTGGAGAAT AAGCTGCAGC 
     4021 AGCAGTCATC ACAGGCAGCC CAGGAGCTTG CAGCAGAGAA AGGAAAACTG TCAGCGCTGC 
     4081 AGAGCAACTA CGAGAAGTGC CAGGCGGACC TGAAACAGCT GCAGTCCGAC CTCTATGGCA 
     4141 AAGAGTCTGA ACTTCTAGCC ACGAGGCAAG ACCTTAAGTC TGTAGAAGAG AAGCTCACTC 
     4201 TGGCACAAGA AGACTTGATT TCAAACAGAA ATCAAATTGG AAACCAAAAT AAATCAATTC 
     4261 AAGAATTGCA AGCCGCCAAA GCTAGTTTGG AGCAGGACTC AGCAAAGAAA GAGGCGCTGC 
     4321 TAAAGGAGCA GAGTAAGGCA CTTGAGGATG CTCAGAGAGA AAAGTCAGTG AAGGAAAAAG 
     4381 AACTAGTAGC TGAAAAGTCT AAATTGGCAG AGATGGAGGA AATTAAATGT AGACAAGAAA 
     4441 AAGAAATCAC AAAACTCAAT GAGGAGCTCA AGTCGCACAA ACAAGAAAGC ATAAAGGAAA 
     4501 TAACAAATCT CAAGGATGCC AAGCAACTTT TGATTCAACA GAAATTAGAA CTTCAAGGCA 
     4561 GAGTGGACTC ACTGAAGGCA GCTCTTGAGC AGGAGAAGGA GAGTCAGCAG CTGATGCGAG 
     4621 AGCAGGTGAA GAAGGAGGAA GAGAAGCGGA AGGAAGAGTT TTCTGAGAAG GAAGCGAAGC 
     4681 TGCATTCTGA AATAAAAGAA AAGGAAGCGG GAATGAAGAA ACACGAAGAA AACGAGGCTA 
     4741 AGCTTACTAT GCAGGTCACA ACATTAAATG AAAACTTGGG TACTGTGAAG AAGGAGTGGC 
     4801 AGTCTAGTCA ACGGAGAGTC AGTGAGCTTG AGAAGCAAAC GGATGACTTA CGGGGAGAAA 
     4861 TTGCTGTCTT AGAAGCAACT GTTCAGAATA ATCAAGACGA AAGAAGAGCA CTCCTAGAGA 
     4921 GGTGTCTTAA AGGCGAAGGT GAAATAGAAA AGCTTCAGAC CAAAGCCCTA GAGCTGCAGA 
     4981 GAAAGCTGGA TAACACAACT GCGGCCGTGC AGGAGCTGGG CAGGGAGAAC CAGTCTCTCC 
     5041 AGATCAAACA CACACAAGCA TTGAACAGAA AGTGGGCGGA AGACAACGAA GTGCAGAACT 
     5101 GCATGTCCTG TGGGAAGTGC TTCTCCGTCA CCGTGCGGCG GCATCACTGC CGACAGTGTG 
     5161 GGAATATCTT CTGTGCCGAG TGCTCAACCA AAAATGCCTT AACTCCTTCT TCGAAGAAGC 
     5221 CTGTTCGTGT CTGTGATGCA TGCTTCAATG ATTTGCAAGG AACGCGTACG CGGCCGCTCG 
     5281 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5341 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5401 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5461 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5521 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5581 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5641 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5701 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5761 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5821 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5881 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5941 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6001 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6061 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6121 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6181 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6241 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6301 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6361 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6421 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6481 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6541 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6601 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6661 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6721 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6781 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6841 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6901 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6961 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7021 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7081 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7141 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7201 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7261 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7321 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7381 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7441 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7501 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7561 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7621 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7681 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7741 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7801 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7861 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7921 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7981 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8041 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8101 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8161 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8221 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8281 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8341 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8401 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8461 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8521 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8581 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8641 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8701 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8761 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8821 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8881 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8941 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9001 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9061 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//