LOCUS       MR211974                9114 bp    DNA     circular  SYN 
DEFINITION  Mlh3 (Myc-DDK-tagged) - Mouse mutL homolog 3 (E coli) (Mlh3)
ACCESSION   NM_175337
VERSION     NM_175337.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5271..5362 
                     /label="Myc-DDK"  
     misc_feature    5392..5977 
                     /label="PolyA signal"  
     misc_feature    5449..5468 
                     /label="Restriction Enzyme"  
     misc_feature    6253..6925 
                     /label="ColE1"  
     misc_feature    7355..8149 
                     /label="Kan/Neo"  
     misc_feature    8184..8486 
                     /label="SV40 ori"  
     misc_feature    8572..9049 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5253..5259 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5253 
                     /label="Mlh3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATCAGGTGT CTATCAGATG ACGTAAAAAC CAAGTTGCGT TCCGGTTTAG 
     1081 CCATAAGCTC CTTGGGCCAG TGTGTTGAAG AACTTACCCT TAACAGTATT GATGCTGAAG 
     1141 CAACATGTGT GGCCATCAGA GTGAATATGG AAACCTTCCA AGTTCAAGTG ATAGACAATG 
     1201 GACTTGGGAT GGCGGGGGAC GATGTAGAGA AGGTGGGAAA CCGGTATTTT ACTAGTAAAT 
     1261 GCCACTCAGT GCGGGACTTG GAGAACCCAG CATTTTATGG CTTCCGAGGA GAGGCCTTGG 
     1321 CAAGTATAGC CGACATGGCT GGTGCTGTGG AGATTTCATC CAAGAAAAAC ACAACACTGA 
     1381 AAACCTTTGT GAAAATGTTT CAGAATGGAA AAGCCCTTGC CACCCATGAG GCTGATTTGA 
     1441 CCAGACCAAG TGTGGGGACT ACAGTAACGG TCTATAACCT GTTTTACCAG TTTCCTGTGC 
     1501 GGAGGAAAAG CATGGATCCT AGACTAGAGT TTGAGAAAGT TCGGCAGAGG GTAGAAGCTC 
     1561 TCTCACTTAT GCACCCCTCC ATTTCTTTCT CTTTGAGGAA CGATGTATCT GGATCCATGG 
     1621 TTCTTCAGCT CCCTAAAACC AAAGACATAT GCTCTCGATT CTGTCAAATT TACGGATTGG 
     1681 GCAAGTCCCA AAAGTTAAGA GAAATACGTT TTAAATACAA GGAATTTGAG TTCAGTGGCT 
     1741 ACATCAGCTC TGAAGCACAC TACAATAAGA ATATGCAGTT TTTGTTTGTG AACAGAAGAC 
     1801 TAGTTTTAAG AACAAAGTTG CATAAACTTA TTGACTTTTT ATTAAGAAAA GAAAGCATTA 
     1861 TATGCAGGCC AAAGAATGGC TCTGCCAGTA GGCAAATGAA TTCAAGTCCT CGACACCGTT 
     1921 CTGCCTCAGA ACTCCACGGG ATATATGTAA TCAATGTGCA GTGCCCTTTC TGTGAGTATG 
     1981 ATGTCTGCAT AGAGCCAGCC AAAACTCTGA TTGAGTTTCA GAGCTGGGAT ACCGTGTTGA 
     2041 TTTGTATTCA GGAAGGAGTA AAAAGGTTTT TAAAGCAAGA AAAATTATTT GTAGAATTAT 
     2101 CAGGTGAAGA TATTAAGGAA TTTAATGAAG ATAATGGTTT TAGTTTGTTT GGCACGACTC 
     2161 TTCAGACACA TGTGTCTACT CATGAGAAGT GTGACCAGAG CAGTTTCCGG GAAGCGTGTA 
     2221 ATAAAATTCT GGATTCCTAT GAAATGTTTA ATTTGCAGTC AAAAGCTGTG AAAAGAATAG 
     2281 CTACTCTAGA AAATAAAACC AGACAAAACC CTGGTGATTC AGAAACTATC AGAAAAAAGA 
     2341 CAGTGGGCTC ATTGTACACA GATGCATCGG ATGGCCCGTG CTATAGTAAA TCGGTAGAGT 
     2401 CTGTTTTACA GGACAGCAAC AACAGTGCTT ACTTAGAACC GAGGGTGTCA GAAGAAGAGG 
     2461 TAGCCAAAAC ATCACACTCC GGAGAAAATG AGAAATGGAA AAAATCTTTT TTGGAAAATA 
     2521 AGACTTCAGG AAGGATACAT GAAACCAGTC CAAAAATGTT TTCAAGCCCC ATCCAAATGC 
     2581 ATCACCTCCT TGAGGAGAGA GAGGCAGATC TGGAAATGCA GACAATAAGT AGTACTGTTA 
     2641 ATGTCATGGC TGCCAACATT CCCCAAAATA ATGACATTCC GAGTCAACTG GAGAAATGGA 
     2701 AAGATGCTCC TGAAGTGGGG TGCCAACCTC TGCCTTTTGA GACAACCTTA TTAAGGGTAC 
     2761 GGGGTACTCA GAGGAAGAAG GAAAGAAGGA AAAAGGAGCC CAGTAGTCGT GGAAGAGTAA 
     2821 ATGTTTTTAG TTATGGACAA GTTAAATTAT GCTCCACTGG CTTTATAACT CATGTAGTAC 
     2881 AAAGTGAGCA CGCTAAATCA ACTGAAACAG AACATTCATT TAAAAATTAT GCTCGACCTG 
     2941 GTCCTGTAAG TGCCCAAGAA ACATTTGGAA AAAGAACACA CCATGCAATT GAGACTCCAG 
     3001 ACAGCAGTGA TTTAACAAGC ACTTTAAGTA AAGAATCCAG TCAACCGCCC AACAAAAGGT 
     3061 TTTGCAGAAC AAATACAGGT TACGGGACAG AGAACAAACC TGTAGCAACA GATGACAACT 
     3121 TGGCTCTTTT TCAGGAAAGC TGTAAAGAAT CACACACAGA TCGCCTTTTG CCTGATGCAT 
     3181 CCTCCTTCCC ATGGTGTAGA TATGTTTCCG ATGGTTGTAG GAAAATAGAT AAAAGGGGTT 
     3241 CCTTCAAACA GGTAGTCCGT AGGAAGCTAA GCTTGCGTTC ACAAGTAGGG TCTTTAGAGA 
     3301 AGTTTAAGAG GCAGTATGGG AAGGTCAGCA GTTCCCTAGA TACAGAAAAG GATAATAACA 
     3361 CTGAAGTCAG GACTCATCTT GATCCTCAAA ATGAACCCGA TGTTCTTCTG AAAGACAAGA 
     3421 GCCACTTAGA TATGTCTGAT GGTTGTGAGA TCACTACTGT GGAGCACAGT GAGACTTGTC 
     3481 AACCATTAAG TCCCATCCTG TACCCAGAAA AAATTTTATT TTCCAAAGAA GATCGCTTAG 
     3541 AACAGATGCC TCATTTGAGA GAAAGTCCTA TAACTCTGGA AGAATTATCT CACTGTAACA 
     3601 GAAAAGCTGA TGTTGAGAAG TCCGCTGCAT CACTGGCTTC TAAATTATCC AAACTAAAGG 
     3661 ATTCTGAAAA AGAGATGCAA ACCGTGGGGA TGACAGGTCA TACTAGTGAA CTTCCAGATT 
     3721 CAAATCCCAG TTGGAAAGAT AATAGCCAGT GCACTAGGTT AGACTTGGAT TTTTGTGAAT 
     3781 TATTAAAAAA CAAACTTGAA AAAATAGAGA GTGATATGCT TCCAATGGCA GATTCTGCCA 
     3841 CAGAGGATGG TCCCATCAAT AAAAACAGTG AACTACATCC TAACAATACA ACGGATGACA 
     3901 CAGAGAAACC AGAAACTCCT TTGCTGTTCC CCTGTAATGA TTCTAAAATC AGCAGAGATT 
     3961 CAGATGTTCT TATCAGAACT TCAGAACAAC CTACAGGAAA CCCTGACTCT GTCGGTAAAG 
     4021 TGATAATGAG TCAGGTAGAG GATGGCATTG GCAGCCAAGG TGGAGTCTGT CCCCAGGGTG 
     4081 ATGAATCTAA GGCAAGATCT TGTTCCAAAA ATGAACCAAA CGCACACTGT ATGGATTGGC 
     4141 AGCAGCATTT TGATGTAACC CTGGGAAGAA TGGTTTACAT CAACAGAATG ACAGGACTTA 
     4201 GCACATTCGT TGCTCCAACT GACGACCTTC ATACTGCTTG TACTAAAGAT CTGACAACTG 
     4261 TGGCTGTGGA TGTCCTGCTT GGGAATGATG CTGTGGATGC TGCTGCTGCT GCTGTCAGTG 
     4321 AACCCCTTCA GTCTCTGTTT TCAGAATGGA GCAATCCAGT GTTTGCTCGA TACCCAGAGG 
     4381 TTGCTGTTGA TGTCAGCAGT GGCCAGGCTG AGAGCTTAGC CGTTAAAATT CACAACGTCC 
     4441 TGTATCCCTA TCGCTTCACC AAAGAGATGA TTCACTCAGT GAAGGTTCTC CAGCAAGTGG 
     4501 ATAACAAGTT TATTGCCTGC TTAATGAGCA CGAGGATGGA TGAGGATGGC CGAACAGGTG 
     4561 GAAACCTGTT AGTCCTGGTG GACCAGCATG CTGCCCATGA ACGCATTCGT TTGGAGCAGC 
     4621 TTATTACTGA TTCCTATGAG AAACAAGATC CACAAAGCGC TGGCCGGAAG AAATTATTGT 
     4681 CTTCCACAAT AATCCCTCCA CTGGCAATCA CCGTGTCAGA GGAACAAAGG AGACTCTTAC 
     4741 GGTCTTACCA CAAACATTTA GAAGATCTGG GGCTTGAGTT GCTCTTTCCA GATGCTAGTG 
     4801 ATTCTCTGAT CCTGGTGGGA AAAGTGCCGC TCTGCTTTGT AGAGAGAGAA GCTAGTGAGC 
     4861 TTCGAAGAGG ACGCTCTACT GTGACTAAGA GTATTGTGGA GGAATTAATT CGAGAACAAG 
     4921 TGGAGCTGCT CCAGACCACA GGAGGTATCC AAGGGACACT GCCACTGACT GTCCAGAAGG 
     4981 TGTTGGCCTC CCAGGCCTGC CATGGGGCTA TTAAGTTTAA TGATCGTCTG AGCCTAGAAG 
     5041 AGAGCTGCCG CCTCATCGAA GCTCTGTCCT TGTCCCAGCT GCCATTTCAG TGTGCTCATG 
     5101 GGAGACCCTC AATGCTGCCC TTAGCTGACC TGGACCACTT GGAGCAGGAA AAACAGGTTA 
     5161 AACCCAACCT TGCTAAACTT CGCAAAATGG TTCGTGCCTG GCATCTCTTT GGAAAAACAG 
     5221 AGCAGAACCT GCAGCAGCCT ATACGTCCTT GTGAGCCTCC AACGCGTACG CGGCCGCTCG 
     5281 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5341 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5401 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5461 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5521 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5581 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5641 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5701 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5761 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5821 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5881 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5941 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6001 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6061 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6121 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6181 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6241 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6301 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6361 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6421 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6481 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6541 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6601 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6661 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6721 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6781 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6841 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6901 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6961 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7021 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7081 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7141 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7201 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7261 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7321 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7381 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7441 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7501 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7561 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7621 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7681 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7741 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7801 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7861 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7921 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7981 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8041 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8101 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8161 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8221 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8281 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8341 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8401 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8461 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8521 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8581 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8641 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8701 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8761 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8821 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8881 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8941 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9001 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9061 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//